LOCUS       sequence-362555-       11067 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual fluorescent reporter construct with double HS4 insulator, 
            upstream TRE (Tetracycline Response Element) recruitment site, 
            phiC31 attB for integration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11067)
  AUTHORS   Lensch S, Herschl MH, Ludwig CH, Sinha J, Hinks MM, Mukund A, 
            Fujimori T, Bintu L
  TITLE     Dynamic spreading of chromatin-mediated gene silencing and 
            reactivation between neighboring genes in single cells.
  JOURNAL   Elife. 2022 Jun 9;11. pii: 75115. doi: 10.7554/eLife.75115.
  PUBMED    35678392
REFERENCE   2  (bases 1 to 11067)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-06-9"; volume: "11. pii: 75115. doi"; pages: " 
            10.7554/eLife.75115"
COMMENT     Sequence Label: 
            pMH004_phiC31_neo-HS4-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
FEATURES             Location/Qualifiers
     source          1..11067
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    16..85
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             351..1154
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(414..433)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1024..1043
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1186..1203)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1192..1416
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    2785..2803
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2821..2839
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2857..2875
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2892..2910
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2928..2946
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     intron          3191..4129
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4086..4106
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        4162..4180
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             4271..4648
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      4667..4676
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4673..4675,4676..4678,4679..5389)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4718..4739)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4979..4998)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5326..5347
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5440..5459)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5441..5459)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    5733..5854
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7196..7458
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     CDS             7472..7849
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      7862..7871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7868..8581
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYKS"
     primer_bind     complement(8013..8031)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8281..8300
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    8697..8778
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        8821..8884
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8885..8953,8954..9745)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9103..9122)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9916..10504
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10405..10424
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    10688..10822
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    10925..11046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
       61 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
      121 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc
      181 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
      241 cgtcagcggg ttctcgacgg tcacggcggg catgtcgacg gtatcgataa gcttacgcgt
      301 gctagcgcat gcagatctac tagtcggccg gataaagata tcaactcgag atgggatcgg
      361 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      421 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
      481 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
      541 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
      601 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
      661 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
      721 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
      781 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
      841 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
      901 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
      961 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1021 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1081 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1141 acgagttctt ctgaggggat caattctcta gagctcgctg atcagcctcg actgtgcctt
     1201 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     1261 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     1321 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     1381 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     1441 gggggctagc aaagcctagg aagacgtaaa ttgtaagcgt gagatccgtt tcgataagct
     1501 ctagagggac agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag
     1561 ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc gcatccccga
     1621 gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct
     1681 gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg gaaaaagctt
     1741 taggctgaaa gagagattta gaatgacaga atcatagaac ggcctgggtt gcaaaggagc
     1801 acagtgctca tccagatcca accccctgct atgtgcaggg tcatcaacca gcagcccagg
     1861 ctgcccagag ccacatccag cctggccttg aatgcctgca gggatggggc atccacagcc
     1921 tccttgggca acctgttcag tgcgtcacca ccctctgggg gaaaaactgc ctcctcatat
     1981 ccaacccaaa cctcccctgt ctcagtgtaa agccattccc ccttgtccta tcaaggggga
     2041 gtttgctgtg acattgttgg tctggggtga cacatgtttg ccaattcagt gcatcacgga
     2101 gaggcagatc ttggggataa ggaagtgcag gacagcatgg acgtgggaca tgcaggtgtt
     2161 gagggctctg ggacactctc caagtcacag cgttcagaac agccttaagg ataagaagat
     2221 aggatagaag gacaaagagc aagttaaaac ccagcatgga gaggagcaca aaaaggccac
     2281 agacactgct ggtccctgtg tctgagcctg catgtttgat ggtgtctgga tgcaagcaga
     2341 aggggtggaa gagcttgcct ggagagatac agctgggtca gtaggactgg gacaggcagc
     2401 tggagaattg ccatgtagat gttcatacaa tcgtcaaatc atgaaggctg gaaaagccct
     2461 ccaagatccc caagaccaac cccaacccac ccaccgtgcc cactggccat gtccctcagt
     2521 gccacatccc cacagttctt catcacctcc agggacggtg acccccccac ctccgtgggc
     2581 agctgtgcca ctgcagcacc gctctttgga gaaggtaaat cttgctaaat ccagcccgac
     2641 cctcccctgg cacaacgtaa ggccattatc tctcatccaa ctccaggacg gagtcagtga
     2701 ggatggggct ctagcggggg gatcaaacgg ccggcctcta gagtcgagat gacattgatt
     2761 attgagtata cgcgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
     2881 tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2941 tagagaacgt atgtcgaggt aggcgtgtac ggtgggaatg catgagcgca catcgcccac
     3001 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     3061 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     3121 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     3181 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     3241 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     3301 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     3361 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     3421 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     3481 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     3541 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     3601 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     3661 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     3721 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     3781 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     3841 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     3901 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     3961 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     4021 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     4081 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagc
     4141 tagcgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tggagctcca
     4201 ccgcggtggc ggccgctcta gcccgggcgg atcccccggg ctgcaggaat tcgatatcaa
     4261 gcttgccacc atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa
     4321 ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag
     4381 ctattccatc tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc
     4441 caaggccatg ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga
     4501 ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac
     4561 ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa
     4621 ggccatcacc aagtacacca gcgctaagga tccccgggta ccggtcgcca ccatggtgag
     4681 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     4741 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     4801 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4861 caccttcggc tacggcctga tgtgcttcgc ccgctacccc gaccacatga agcagcacga
     4921 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     4981 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5041 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5101 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5161 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5221 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5281 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5341 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aggtcgacct
     5401 cgaggggggg cccggtacca ggtaagtgta cccaattcgc cctatagtga gtcgtattac
     5461 aattcactcg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gagatccaat
     5521 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattcaca
     5581 gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc
     5641 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     5701 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     5761 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5821 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgtctcagta
     5881 aattgtaagc gtgagatccg tttcgataag ctctagaggg acagcccccc cccaaagccc
     5941 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     6001 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     6061 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     6121 cagacacctg gggggatacg gggaaaaagc tttaggctga aagagagatt tagaatgaca
     6181 gaatcataga acggcctggg ttgcaaagga gcacagtgct catccagatc caaccccctg
     6241 ctatgtgcag ggtcatcaac cagcagccca ggctgcccag agccacatcc agcctggcct
     6301 tgaatgcctg cagggatggg gcatccacag cctccttggg caacctgttc agtgcgtcac
     6361 caccctctgg gggaaaaact gcctcctcat atccaaccca aacctcccct gtctcagtgt
     6421 aaagccattc ccccttgtcc tatcaagggg gagtttgctg tgacattgtt ggtctggggt
     6481 gacacatgtt tgccaattca gtgcatcacg gagaggcaga tcttggggat aaggaagtgc
     6541 aggacagcat ggacgtggga catgcaggtg ttgagggctc tgggacactc tccaagtcac
     6601 agcgttcaga acagccttaa ggataagaag ataggataga aggacaaaga gcaagttaaa
     6661 acccagcatg gagaggagca caaaaaggcc acagacactg ctggtccctg tgtctgagcc
     6721 tgcatgtttg atggtgtctg gatgcaagca gaaggggtgg aagagcttgc ctggagagat
     6781 acagctgggt cagtaggact gggacaggca gctggagaat tgccatgtag atgttcatac
     6841 aatcgtcaaa tcatgaaggc tggaaaagcc ctccaagatc cccaagacca accccaaccc
     6901 acccaccgtg cccactggcc atgtccctca gtgccacatc cccacagttc ttcatcacct
     6961 ccagggacgg tgaccccccc acctccgtgg gcagctgtgc cactgcagca ccgctctttg
     7021 gagaaggtaa atcttgctaa atccagcccg accctcccct ggcacaacgt aaggccatta
     7081 tctctcatcc aactccagga cggagtcagt gaggatgggg ctctagcggg gggatcaaac
     7141 ggccggcctc tagagtcgag atgacattga ttattgacct agggacgtcc atatgaatgt
     7201 agtcttatgc aatacacttg tagtcttgca acatggtaac gatgagttag caacatgcct
     7261 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     7321 ccttattagg aaggcaacag acaggtctga catggattgg acgaaccact gaattgcgca
     7381 ttgcagagat aattgtattt aagtgcctag ctcgatacaa taaacgccat ttgaccattc
     7441 accacattgg tgtgcaccct gcagatctag aatgccagag ccagcgaagt ctgctcccgc
     7501 cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg
     7561 caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca
     7621 ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat
     7681 tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat
     7741 cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca
     7801 cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg atccaccggt
     7861 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     7921 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     7981 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     8041 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     8101 gaagcacccc gccgacattc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     8161 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     8221 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     8281 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     8341 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     8401 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     8461 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     8521 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtctta
     8581 agcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     8641 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     8701 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8761 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatctcata
     8821 tgtgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8881 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8941 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9001 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9061 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9121 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9181 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9241 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9301 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9361 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9421 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9481 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9541 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9601 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9661 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9721 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9781 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9841 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9901 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9961 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10021 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10081 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10141 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10201 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10261 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10321 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10381 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10441 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10501 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10561 catgttcttt cctgcggtac cgatcataat cagccatacc acatttgtag aggttttact
    10621 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    10681 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10741 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10801 tgtatcttat catgtctgga tcataatcag ccataccaca tttgtagagg ttttacttgc
    10861 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    10921 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    10981 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11041 atcttatcat gtctgctcga ggtcgac
//
