LOCUS       sequence-36256-e        3992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3992)
  AUTHORS   Schowalter RM, Pastrana DV, Buck CB
  TITLE     Glycosaminoglycans and sialylated glycans sequentially facilitate 
            merkel cell polyomavirus infectious entry.
  JOURNAL   PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28.
  PUBMED    21829355
REFERENCE   2  (bases 1 to 3992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2011-07"; volume: "7(7)"; pages: "e1002161. Epub 
            2011 Jul 28"
COMMENT     Sequence Label: pMtB
FEATURES             Location/Qualifiers
     source          1..3992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          449..1396
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     misc_feature    2028..2472
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        2506..2552
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        2544..2562
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    3024..3145
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3035..3054)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3089..3108
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      3333..3921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3822..3841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggctga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagttc cgccagagcg cgcgagggcc tccagcggcc gcccctcccc cacagcaggg
      301 gcggggtccc gcgcccaccg gaaggagcgg gctcggggcg ggcggcgctg attggccggg
      361 gcgggcctga cgccgacgcg gctataagag accacaagcg acccgcaggg ccagacgttc
      421 ttcgccgaag cttgccgtca gaacgcaggt gaggggcggg tgtggcttcc gcgggccgcc
      481 gagctggagg tcctgctccg agcgggccgg gccccgctgt cgtcggcggg gattagctgc
      541 gagcattccc gcttcgagtt gcgggcggcg cgggaggcag agtgcgaggc ctagcggcaa
      601 ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt ggtgtccgcg ccgccgccgc
      661 gtgctactcc ggccgcactc tggtcttttt tttttttgtt gttgttgccc tgctgccttc
      721 gattgccgtt cagcaatagg ggctaacaaa gggagggtgc ggggcttgct cgcccggagc
      781 ccggagaggt catggttggg gaggaatgga gggacaggag tggcggctgg ggcccgcccg
      841 ccttcggagc acatgtccga cgccacctgg atggggcgag gcctggggtt tttcccgaag
      901 caaccaggct ggggttagcg tgccgaggcc atgtggcccc agcacccggc acgatctggc
      961 ttggcggcgc cgcgttgccc tgcctcccta actagggtga ggccatcccg tccggcacca
     1021 gttgcgtgcg tggaaagatg gccgctcccg ggccctgttg caaggagctc aaaatggagg
     1081 acgcggcagc ccggtggagc gggcgggtga gtcacccaca caaaggaaga gggcctggtc
     1141 cctcaccggc tgctgcttcc tgtgaccccg tggtcctatc ggccgcaata gtcacctcgg
     1201 gcttttgagc acggctagtc gcggcggggg gaggggatgt aatggcgttg gagtttgttc
     1261 acatttggtg ggtggagact agtcaggcca gcctggcgct ggaagtcatt tttggaattt
     1321 gtccccttga gttttgagcg gagctaattc tcgggcttct tagcggttca aaggtatctt
     1381 ttaaaccctt ttttaggtgt tgtgaaaacc accgctaatt caaagcaacc ggtgatatcg
     1441 gatccacgcg taagatggat ttagtcctaa ataggaaaga aagagaggct ctctgcaagc
     1501 ttttagagat tgctcctaat tgttatggca acatccctct gatgaaagct gctttcaaaa
     1561 gaagctgctt aaagcatcac cctgataaag ggggaaatcc tgttataatg atggaattga
     1621 acaccctttg gagcaaattc cagcaaaata tccacaagct cagaagtgac ttctctatgt
     1681 ttgatgaggt cagtacaaaa tttccttggg aagaatatgg aactttaaag gattatatgc
     1741 aaagtggata taatgctaga ttttgcagag gtcctgggtg catgcttaag caacttagag
     1801 attctaagtg cgcttgtatt agctgtaagt tgtctcgcca gcattgtagt ctaaaaactt
     1861 taaagcaaaa aaactgtctg acgtggggag agtgtttttg ctatcagtgc tttattcttt
     1921 ggtttggatt tcctcctact tgggaaagtt ttgactggtg gcaaaaaact ttagaagaaa
     1981 ctgactactg cttactgcat ctgcaccttt tctgagtcga ccctaggagc aggtttcccc
     2041 aatgacacaa aacgtgcaac ttgaaactcc gcctggtctt tccaggtcta gaggggtaac
     2101 actttgtact gcgtttggct ccacgctcga tccactggcg agtgttagta acagcactgt
     2161 tgcttcgtag cggagcatga cggccgtggg aactcctcct tggtaacaag gacccacggg
     2221 gccaaaagcc acgcccacac gggcccgtca tgtgtgcaac cccagcacgg cgactttact
     2281 gcgaaaccca ctttaaagtg acattgaaac tggtacccac acactggtga caggctaagg
     2341 atgcccttca ggtaccccga ggtaacacgc gacactcggg atctgagaag gggactgggg
     2401 cttctataaa agcgctcggt ttaaaaagct tctatgcctg aataggtgac cggaggtcgg
     2461 cacctttcct ttgcaattac tgaccctatg aatacaactg actgtttgac aattaatcat
     2521 cggcatagta tatcggcata gtataatacg actcactata ggagggccac catgaagacc
     2581 ttcaacatct ctcagcagga tctggagctg gtggaggtcg ccactgagaa gatcaccatg
     2641 ctctatgagg acaacaagca ccatgtcggg gcggccatca ggaccaagac tggggagatc
     2701 atctctgctg tccacattga ggcctacatt ggcagggtca ctgtctgtgc tgaagccatt
     2761 gccattgggt ctgctgtgag caacgggcag aaggactttg acaccattgt ggctgtcagg
     2821 cacccctact ctgatgaggt ggacagatcc atcagggtgg tcagcccctg tggcatgtgc
     2881 agagagctca tctctgacta tgctcctgac tgctttgtgc tcattgagat gaatggcaag
     2941 ctggtcaaaa ccaccattga ggaactcatc cccctcaagt acaccaggaa ctaaacctga
     3001 attcgctagc tggccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     3061 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     3121 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
     3181 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatgga
     3241 aatgttaatt aactagccat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3301 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3361 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3421 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3481 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3541 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3601 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3661 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3721 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3781 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3841 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3901 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3961 tgctggcctt ttgctcacat gttcttaatt aa
//
