LOCUS       sequence-362563-        6766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression of mouse RAP1 delta COIL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Barry RM, Sacco O, Mameri A, Stojaspal M, Kartsonis W, Shah P, De 
            Ioannes P, Hofr C, Cote J, Sfeir A
  TITLE     Rap1 regulates TIP60 function during fate transition between 
            two-cell-like and pluripotent states.
  JOURNAL   Genes Dev. 2022 Feb 24. pii: gad.349039.121. doi: 
            10.1101/gad.349039.121.
  PUBMED    35210222
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2022 Feb 24. pii: gad.349039.121. doi: 10.1101/gad.349039.121."
COMMENT     Sequence Label: pLPC-MYC.Mm.RAP1.delta.COIL
FEATURES             Location/Qualifiers
     source          1..6766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(930..1721,1722..1790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1553..1572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1791..1895)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1926..2395
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2460..2659
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             2860..3276
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3206..3228
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3244..3260
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3294..3893
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3294..3313)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3790..3810
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(3982..4001)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4173..4552
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4553..4756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4707..4727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4801..4820
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4801..4819
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4851..4860
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4860..4889
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     LTR             6178..6647
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccgcaa atcaatctaa
      901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1921 gcagcctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     1981 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     2041 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
     2101 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     2161 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     2221 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
     2281 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     2341 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcatttgg
     2401 gggctcgtcc gggatcggga gacccctgcc cagggaccac cgacccacca ccgggaggca
     2461 agctggccag caacttatct gtgtctgtcc gattgtctag tgtctatgac tgattttatg
     2521 cgcctgcgtc ggtactagtt agctaactag ctctgtatct ggcggacccg tggtggaact
     2581 gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt tgggggccgt
     2641 ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc aggatatgtg
     2701 gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt tttgctttcg
     2761 gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt tctgtgttgt
     2821 ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt taccactccc
     2881 ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta
     2941 gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc
     3001 ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc
     3061 ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc
     3121 ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct
     3181 cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc
     3241 tccctttatc cagccctcac tccttctcta ggcgccggcc ggatcagctt accatgaccg
     3301 agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc
     3361 tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca
     3421 tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca
     3481 aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg
     3541 aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc
     3601 tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt
     3661 ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg
     3721 tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct
     3781 ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg
     3841 aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc
     3901 cccacgaccc gcagcgcccg accgaaagga gcgcacgacc ccatgcatcg gatctcccga
     3961 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     4021 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     4081 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     4141 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     4201 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     4261 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     4321 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     4381 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     4441 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     4501 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     4561 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     4621 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     4681 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     4741 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
     4801 taatacgact cactataggg agacccaagc ttggtaccga gctcggatca gccaccatgg
     4861 aacaaaagtt gatttctgaa gaagatttgt taagaaagag aggatccgcg gaggcgatgg
     4921 atctgggtaa agaccccaat gggcccactc actcctccac tctgttcgtg agagaagacg
     4981 gcagcgccat gtcgttttac gtgcggccca gctcggccaa gcgccggctg tcgacgctca
     5041 tcctgcacgg cggcggcacc gtgtgtcggg tgcaggaacc cggagccgtg cttctcgccc
     5101 agcccgggga ggcgctggcc gaggcttcgg gcgacttcat ctccacgcag tacatcctag
     5161 actgcgtgga tcgcaacgag aagctggacc tggaggccta tcggctgggc ctgacggagc
     5221 aggcgtccga tccgaagccc ggggcttcca ccgagggctc cacggaaccg gagccgcagc
     5281 ccctgaccgg gcgcatcgcc tacaccgacg cggaagatgt ggccatcctg acctacgtga
     5341 aggagaacgc ccgttcgccc agctcggtca caggcaatgc cttgtggaaa gcgatggaga
     5401 agagctcgct cacgcagcac tcctggcagt cgctcaagga ccgctacctc aagcacctac
     5461 ggggtcagga gcacaagtac ctgctcggga acgccccggt cagcccgtcc tcccagaagc
     5521 tcaaacggaa ggcggagcag gaccccgagg ccgcggatag cggagagcca cagaacaaga
     5581 gagcgccaga cttgctgcag gagagccctg actttgaaat acatataacg atgtgtgatg
     5641 gtgatccacc cacacccgag gaagactcag aaacacagcc agacgaggag gaagaagaac
     5701 caaaagtttc tacgcaagaa gtgggaactg ccattaaggt gatccggcag ctaatggaga
     5761 agttcaactt ggatctatca acagttacac aggccttgct gaagaacagt ggtgagctgg
     5821 aggccacgtc ctccttctta gagtcggggc ggagacccga cggttatccc atttggtgca
     5881 gacaagatga cttagatttg cagaaggacg atgacgacac gaaaaatgca ctggtcaaaa
     5941 agtttggagc tcagaacgtt gctcggagga ttgaattccg aaagaaataa ctcgagcatg
     6001 catctagagg gccgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     6061 aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga
     6121 aaaatacata actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg
     6181 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     6241 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     6301 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga
     6361 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     6421 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     6481 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     6541 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     6601 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta
     6661 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa
     6721 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgct
//
