LOCUS       sequence-362577-        8636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Big-IN Landing Pad plasmid encoding pEF1a-driven 
            mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette 
            flanked by heterotypic LoxM/LoxP sites and BsaI homology arms 
            cloning sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8636)
  AUTHORS   Brosh R, Laurent JM, Ordonez R, Huang E, Hogan MS, Hitchcock AM, 
            Mitchell LA, Pinglay S, Cadley JA, Luther RD, Truong DM, Boeke JD, 
            Maurano MT
  TITLE     A versatile platform for locus-scale genome rewriting and 
            verification.
  JOURNAL   Proc Natl Acad Sci U S A. 2021 Mar 9;118(10). pii: 2023952118. doi: 
            10.1073/pnas.2023952118.
  PUBMED    33649239
REFERENCE   2  (bases 1 to 8636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2021-03-9"; volume: "118(10). pii: 
            2023952118. doi"; pages: " 10.1073/pnas.2023952118"
COMMENT     Sequence Label: pLP-PIGA (pLP140)
FEATURES             Location/Qualifiers
     source          1..8636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..34)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     promoter        61..1239
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          292..1230
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1187..1207
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1250..1945
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(1383..1401)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             1961..2017
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2024..3052
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             3065..4000
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             4019..4075
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4082..4678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4082..4101)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4578..4598
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4688..4744
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(6673..6706)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(6841..7632,7633..7701))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7464..7483
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7702..7806)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7909..8497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8398..8417
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataacttcgt ataggatact ttatacgaag ttatacgcgc ctgcagcaga cgtaagtcgg
       61 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
      121 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      181 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      241 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      301 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      361 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      421 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      481 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      541 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      601 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      661 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      721 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      781 agtctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      841 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      901 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      961 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgctgtccag gcacctcgat
     1021 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1081 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1141 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1201 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag agcccatgga tggtgagcaa
     1261 gggcgaggca gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa
     1321 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac
     1381 cgccaagctg aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc
     1441 tcagttcatg tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta
     1501 taagcagtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg
     1561 cgccgtgacc gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa
     1621 gctccgcggc accaacttcc ctcctgacgg ccccgtaatg cagaagaaaa caatgggctg
     1681 ggaagcgtcc accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat
     1741 ggccctgcgc ctgaaggacg gcggccgcta cctggcggac ttcaagacca cctacaaggc
     1801 caagaagccc gtgcagatgc ccggcgccta caacgttgac cgcaagttgg acatcacctc
     1861 ccacaacgag gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac
     1921 cggcggcatg gacgagctgt acaagggatc cggaagcggt gccaccaact ttagcttgct
     1981 taagcaggcg ggcgacgtgg aggagaatcc cgggccggaa ttcatggcca atttactgac
     2041 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
     2101 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
     2161 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
     2221 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
     2281 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg
     2341 accaagtgac agcaatgctg tttcactggt tatgcggcgc atccgaaaag aaaacgttga
     2401 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
     2461 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat
     2521 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
     2581 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
     2641 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc
     2701 cgtgtctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg
     2761 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga
     2821 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg
     2881 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
     2941 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
     3001 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc
     3061 atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc
     3121 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga
     3181 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc
     3241 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc
     3301 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttcttgaatg
     3361 tgcctggcta gagatcctga tgattggcct cgtctggcgc tctatggagc acccagtgaa
     3421 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat
     3481 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca
     3541 aggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt
     3601 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa
     3661 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc aacagcagca
     3721 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg
     3781 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct
     3841 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga
     3901 ggaaacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcagaa
     3961 gtattacatc acgggggagg cagagggttt ccctgccaca gcttctagag gctcaggcgc
     4021 caccaacttt agcctgctga agcaggcagg cgacgtcgag gagaaccccg gccctgtcga
     4081 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4141 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4201 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4261 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4321 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4381 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4441 gcccgcgtgg ttcctggcca ccgtcggcgt gtcgcccgac caccagggca agggtctggg
     4501 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4561 ggaaacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4621 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg
     4681 cagcggcgcc accaacttca gcctgctgaa gcaggccggc gacgtggagg agaaccccgg
     4741 gcccgcctgt agaggaggag ctgggaatgg ccaccgtgcc tcagctacac tctctcgggt
     4801 tagccctgga agtctttaca catgtagaac ccgtacccat aatatatgca tggtatctga
     4861 ctttttctac ccaaatatgg gaggcgtgga aagccacatt taccagctct ctcagtgcct
     4921 gattgaaaga gggcataagg ttataattgt cacccatgct tatggaaatc gaaaaggcat
     4981 ccgttacctc accagtggcc tcaaagtcta ttacttgcct ctgaaagtca tgtacaacca
     5041 gtctacagcc acgaccctct ttcacagtct gccattgctc aggtacatat ttgttcggga
     5101 gagagtcacg ataatccatt cacatagttc tttttctgct atggcccatg atgctctctt
     5161 ccacgccaag acaatggggc ttcagacagt gttcacggac cattcccttt ttggatttgc
     5221 tgatgtcagc tcggtgctta caaacaagct tctaaccgtg tctctttgtg atacaaacca
     5281 catcatttgt gtgtcttata ctagtaagga aaatactgta ctaagagcag cactgaatcc
     5341 tgaaatagtg tccgtcattc ctaatgctgt agatcctact gacttcactc cagacccatt
     5401 tagaaggcat gatagtataa ctattgttgt tgtcagcaga cttgtttaca gaaaagggat
     5461 cgatttgctt agtggtataa tacctgaact ctgtcagaaa tatccagatt taaatttcat
     5521 aattggagga gagggaccaa agagaatcat tttggaagaa gttcgggaaa gataccagct
     5581 gcatgacagg gtgcgtcttt tgggagcttt agaacacaag gatgttagaa atgtcttagt
     5641 tcaaggacat atttttctga atacctccct tactgaagca ttctgcatgg cgatcgtgga
     5701 agcagccagt tgtggtttac aggttgtaag taccagagtt ggtgggattc ctgaggtgct
     5761 tccagaaaac cttattattt tatgtgagcc ttcagtaaaa tctttgtgtg aaggattgga
     5821 aaaggctatt ttccaactga agtcagggac attgccagct ccagaaaaca tccataacat
     5881 agtaaagact ttctacacct ggaggaatgt tgcagaaaga actgaaaagg tatatgaccg
     5941 ggtatcagtg gaagctgtgt tgccaatgga caaacgactg gacagactta tttctcactg
     6001 cggcccagta acaggctaca tctttgcttt gttggcagtt ttcaacttcc tcttcctcat
     6061 tttcttgaga tggatgactc cagattctat cattgatgtt gcaatagatg ccactgggcc
     6121 acggggtgcc tggactaata actattctca cagtaaaaga gggggtgaga ataatgagat
     6181 atctgaaacc aggtaaggta cctgaggcca accttctatc agagttaaac ttttgacaag
     6241 ggaacaaatc tcaaactgat ccatcagtca tgtagctagc tgtagagctt gcaacttaat
     6301 agcagcagct gcccaatgcc atgtgaagta acaaactggt ttttggtttt tttttcccct
     6361 tcagttttaa tgttatgtgt aatgtattta aacccttatt taaataaaac ttgttttcag
     6421 aaatacctga cttgcagatt atttatatat agtcgatctt gtgcatataa aggaaccata
     6481 gaaatgggca gtggaggatt cgtccacacc cacagcttct caacgggcct gaatttggcc
     6541 aagaaaaacc tttatgagct tgtccctctc tggtcaactg gttttttgtt cgtttgtttt
     6601 ttgagacatt cattgcaata gtgtgttgga attttttgtg tctctcactc ggtgcgtcct
     6661 gcaggcgcgt tcataacttc gtatagcata cattatacga agttattatg cgagacctta
     6721 tacgcacggt ctcaatccgc ggccgcgatt atcaaaaagg atcttcacct agatcctttt
     6781 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     6841 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     6901 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     6961 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
     7021 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     7081 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     7141 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     7201 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     7261 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     7321 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     7381 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     7441 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     7501 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     7561 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     7621 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     7681 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     7741 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     7801 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     7861 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7921 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7981 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     8041 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     8101 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     8161 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     8221 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     8281 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     8341 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     8401 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     8461 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     8521 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     8581 ttctgtggat aaccgtgcgg ccgcccctcg agaccttata cgcacggtct caaacg
//
