LOCUS       sequence-36258-e        5947 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Schowalter RM, Pastrana DV, Buck CB
  TITLE     Glycosaminoglycans and sialylated glycans sequentially facilitate 
            merkel cell polyomavirus infectious entry.
  JOURNAL   PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28.
  PUBMED    21829355
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2011-07"; volume: "7(7)"; pages: "e1002161. Epub 
            2011 Jul 28"
COMMENT     Sequence Label: pADL*
FEATURES             Location/Qualifiers
     source          1..5947
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        51..242
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     intron          449..1396
                     /label=mEF-1-alpha intron
                     /note="intron upstream of the start codon of mouse 
                     EF-1-alpha"
     CDS             3925..3966
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3989..4433
                     /label=FMDV IRES
                     /note="internal ribosome entry site (IRES) of the 
                     foot-and-mouth disease virus"
     promoter        4467..4513
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4505..4523
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4533..4907
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4966..5100
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4990..5009)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5044..5063
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      5288..5876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5777..5796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcagggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt ctgaggctga
       61 aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccactagttc cgccagagcg cgcgagggcc tccagcggcc gcccctcccc cacagcaggg
      301 gcggggtccc gcgcccaccg gaaggagcgg gctcggggcg ggcggcgctg attggccggg
      361 gcgggcctga cgccgacgcg gctataagag accacaagcg acccgcaggg ccagacgttc
      421 ttcgccgaag cttgccgtca gaacgcaggt gaggggcggg tgtggcttcc gcgggccgcc
      481 gagctggagg tcctgctccg agcgggccgg gccccgctgt cgtcggcggg gattagctgc
      541 gagcattccc gcttcgagtt gcgggcggcg cgggaggcag agtgcgaggc ctagcggcaa
      601 ccccgtagcc tcgcctcgtg tccggcttga ggcctagcgt ggtgtccgcg ccgccgccgc
      661 gtgctactcc ggccgcactc tggtcttttt tttttttgtt gttgttgccc tgctgccttc
      721 gattgccgtt cagcaatagg ggctaacaaa gggagggtgc ggggcttgct cgcccggagc
      781 ccggagaggt catggttggg gaggaatgga gggacaggag tggcggctgg ggcccgcccg
      841 ccttcggagc acatgtccga cgccacctgg atggggcgag gcctggggtt tttcccgaag
      901 caaccaggct ggggttagcg tgccgaggcc atgtggcccc agcacccggc acgatctggc
      961 ttggcggcgc cgcgttgccc tgcctcccta actagggtga ggccatcccg tccggcacca
     1021 gttgcgtgcg tggaaagatg gccgctcccg ggccctgttg caaggagctc aaaatggagg
     1081 acgcggcagc ccggtggagc gggcgggtga gtcacccaca caaaggaaga gggcctggtc
     1141 cctcaccggc tgctgcttcc tgtgaccccg tggtcctatc ggccgcaata gtcacctcgg
     1201 gcttttgagc acggctagtc gcggcggggg gaggggatgt aatggcgttg gagtttgttc
     1261 acatttggtg ggtggagact agtcaggcca gcctggcgct ggaagtcatt tttggaattt
     1321 gtccccttga gttttgagcg gagctaattc tcgggcttct tagcggttca aaggtatctt
     1381 ttaaaccctt ttttaggtgt tgtgaaaacc accgctaatt caaagcaacc ggtgatatca
     1441 tggatttagt cctaaatagg aaagaaagag aggctctctg caagctttta gagattgctc
     1501 ctaattgtta tggcaacatc cctctgatga aagctgcttt caaaagaagc tgcttaaagc
     1561 atcaccctga taaaggggga aatcctgtta taatgatgga attgaacacc ctttggagca
     1621 aattccagca aaatatccac aagctcagaa gtgacttctc tatgtttgat gaggttgacg
     1681 aggcccctat atatgggacc actaaattca aagaatggtg gagatcagga ggattcagct
     1741 tcgggaaggc atatgaatat gggcccaatc cacacgggac caactcaaga tccagaaagc
     1801 cttcctccaa tgcatccagg ggagccccca gtggaagctc accaccccac agccagagct
     1861 cttcctctgg gtatgggtcc ttctcagcgt cccaggcttc agactcccag tccagaggac
     1921 ccgatatacc tcccgaacac catgaggagc ccacctcatc ctctggatcc agtagcagag
     1981 aggagaccac caattcagga agagaatcca gcacacccaa tggaaccagt gtacctagaa
     2041 attcttccag aacggatggc acctgggagg atctcttctg cgatgaatca ctttcctccc
     2101 ctgagcctcc ctcgtcctct gaggagcctg aggagccccc ctcctcaaga agctcgcccc
     2161 ggcagccccc gtcttcctct gccgaggagg cctcgtcatc tcagtttaca gatgaggaat
     2221 acagatcctc ctccttcacc accccgaaga cccctcctcc attctcaaga aagcgaaaat
     2281 ttggggggtc ccgaagctct gcaagctctg ctagttcagc aagttttaca agcactccac
     2341 caaagccaaa aaagaacaga gaaactcctg ttcctactga ttttcctatt gatctttctg
     2401 attatcttag ccatgctgtg tacagtaata aaacagtctc ctgttttgcc atttatacta
     2461 cttctgataa agctatagag ttatatgata agattgagaa atttaaagtt gattttaaaa
     2521 gcaggcatgc ctgtgaatta ggatgtattt tattgtttat aactttatca aagcatagag
     2581 tatctgctat taagaatttt tgctctacct tctgcactat aagcttttta atttgtaaag
     2641 gagtgaataa gatgcctgaa atgtataata atttatgcaa gcccccttac aaattactgc
     2701 aagagaataa gccactgcta aattatgaat ttcaagaaaa agaaaaagag gccagctgta
     2761 attggaattt agttgctgaa tttgcttgtg aatatgagct agacgaccac tttattatct
     2821 tagcccatta tctagacttt gcaaaaccat ttccttgcca aaagtgtgaa aacagatctc
     2881 gcctcaaacc tcacaaggct catgaggctc atcattctaa tgctaagcta ttttatgaat
     2941 ctaaatctca gaaaaccatt tgccaacaag ccgcagacac tgttctagcc aaaaggaggt
     3001 tagagatgct ggaaatgacc aggacagaaa tgctatgtaa gaagtttaag aagcacctag
     3061 agagattaag agatttagat acaatagatc tactgtatta tatgggtggt gtggcctggt
     3121 actgctgctt atttgaagag tttgaaaaga agctgcagaa aattattcaa ttattaacag
     3181 agaatatacc taagtataga aacatttggt ttaaagggcc tattaacagt ggaaaaacaa
     3241 gctttgctgc agccttaata gatttgctag aagggaaggc cttgaatata aactgtccat
     3301 ctgataagct gccttttgaa ctaggatgtg ctttggataa atttatggtt gtttttgagg
     3361 atgtgaaagg gcaaaatagc ctaaataaag atctgcaacc agggcaagga ataaataacc
     3421 ttgataactt aagagatcat ctagatggtg ctgtagctgt aagcttagag aagaagcatg
     3481 tgaataaaaa gcatcagatt tttcctcctt gtattgttac tgctaatgat tattttattc
     3541 ccaaaacatt aatagcaaga tttagttata ctttacactt cagcccaaaa gcaaatcttc
     3601 gcgattccct ggatcagaac atggaaataa gaaaaagaag aattcttcaa agtggaacca
     3661 ctttattgct ttgtcttatt tggtgcttgc ctgatacaac ctttaagcct tgcttacaag
     3721 aagaaattaa aaactggaag caaattttac agagtgaaat atcatatggt aaattttgtc
     3781 aaatgataga aaatgtagaa gctggtcagg accctctgct caatattctt attgaggaag
     3841 agggccctga ggaaactgaa gaaacccaag attctggtac tttttctcaa taatagctcg
     3901 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     3961 tctacgcgta tcgattgtcg accctaggag caggtttccc caatgacaca aaacgtgcaa
     4021 cttgaaactc cgcctggtct ttccaggtct agaggggtaa cactttgtac tgcgtttggc
     4081 tccacgctcg atccactggc gagtgttagt aacagcactg ttgcttcgta gcggagcatg
     4141 acggccgtgg gaactcctcc ttggtaacaa ggacccacgg ggccaaaagc cacgcccaca
     4201 cgggcccgtc atgtgtgcaa ccccagcacg gcgactttac tgcgaaaccc actttaaagt
     4261 gacattgaaa ctggtaccca cacactggtg acaggctaag gatgcccttc aggtaccccg
     4321 aggtaacacg cgacactcgg gatctgagaa ggggactggg gcttctataa aagcgctcgg
     4381 tttaaaaagc ttctatgcct gaataggtga ccggaggtcg gcacctttcc tttgcaatta
     4441 ctgaccctat gaatacaact gactgtttga caattaatca tcggcatagt atatcggcat
     4501 agtataatac gactcactat aggagggcca tcatggccaa gttgaccagt gccgttccgg
     4561 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     4621 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     4681 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     4741 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     4801 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     4861 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgaccg acgccgacca
     4921 acaccgccgg tccgacgcgg cccgacgggt ccgagggggg tcgacgatcc agacatgata
     4981 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     5041 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     5101 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     5161 taaagcaagt aaaacctcta caaatgtggt atggaaatgt taattaacta gccatgacca
     5221 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5281 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5341 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5401 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5461 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5521 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5581 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5641 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5701 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5761 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5821 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5881 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5941 taattaa
//
