LOCUS       sequence-362580-        5519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Legionella pneumophila SidK (1-278) - mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Maxson ME, Abbas YM, Wu JZ, Plumb JD, Grinstein S, Rubinstein JL
  TITLE     Detection and quantification of the vacuolar H+ATPase using the 
            Legionella effector protein SidK.
  JOURNAL   J Cell Biol. 2022 Mar 7;221(3). pii: 212963. doi: 
            10.1083/jcb.202107174. Epub 2022 Jan 13.
  PUBMED    35024770
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2022-03-7"; volume: "221(3). pii: 212963. doi"; 
            pages: " 10.1083/jcb.202107174. Epub 2022 Jan 13"
COMMENT     Sequence Label: pYMA1_SidK_mCH
FEATURES             Location/Qualifiers
     source          1..5519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             547..1254
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(692..710)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     960..979
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    1376..1497
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1413..1432)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1467..1486
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(1504..1959)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1641..1662)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1853..1872
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        1986..2090
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2092..2449
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2092..2283
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2115..2135)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      2300..2435
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2484..3278
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             2484..3278
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2538..2557)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3148..3167
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(3466..3485)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    3510..3557
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3886..4474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4375..4394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        4649..4952
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4953..5156
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5107..5127
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ccctttaaaa tgaaattaga cctgattgag tctcaatatg agaaaatgaa gcagcatgtg
       61 aatttatgga aaagtgatta ccatgtaaaa ctaaatcaac tgaatcaatt aacagattac
      121 ctcaaaaatg cagcaccaac tcctaaaaat aattttctaa gagcaatgac ctctgtattg
      181 cagatgcaga tagcccaata tgggattaca gaagataatg aaggcatcaa ccagttgttt
      241 aaactgggat tgcatttgct cgcaatggct aacgaaaaaa tagatgaaca atatcacctg
      301 tttaaagggt atgtaaaaga tcagcctgaa gaatctcctt ttgaaggtat tttgcctgcg
      361 gaagaccaga aaattttagt taaaactatg atagattatg caatgccgaa gctttcaagc
      421 aaggttttac aagataaatt atcggcacta tccagttctg atgtattaac aaaaacatta
      481 cttgatagca tcgacagaat tgttaaagaa aacgaaaaat taaatgcttt aagcaaaggg
      541 gatccgatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
      601 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
      661 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
      721 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
      781 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
      841 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
      901 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
      961 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     1021 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     1081 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     1141 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     1201 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggcggcc
     1261 gcgactctag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa
     1321 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt
     1381 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     1441 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg
     1501 cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     1561 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     1621 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     1681 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     1741 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     1801 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     1861 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     1921 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa
     1981 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     2041 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg
     2101 aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     2161 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     2221 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     2281 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     2341 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     2401 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag
     2461 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     2521 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     2581 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     2641 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga
     2701 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     2761 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     2821 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     2881 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     2941 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     3001 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     3061 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     3121 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     3181 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     3241 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     3301 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     3361 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     3421 ggatctcatg ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga
     3481 gacaataccg gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg
     3541 tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac
     3601 cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc
     3661 cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat
     3721 agcctcaggt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     3781 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     3841 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     3901 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     3961 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4021 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4081 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4141 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4201 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4261 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     4321 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     4381 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     4441 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     4501 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     4561 tgtggataac cgtattaccg ccatgcatta gttattaata gtaatcaatt acggggtcat
     4621 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     4681 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     4741 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     4801 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     4861 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     4921 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     4981 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     5041 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     5101 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctggtt
     5161 tagtgaaccg tcagatccgc tagcgctacc ggactcagat ctcgagctca agcttcgaat
     5221 tcatgtcttt tatcaaggta ggtataaaaa tgggtggctt aactagcgag caatatcata
     5281 gtcaagtagt cggtaaaatc ggatatatcg ccagatgcat gcaaactata gatcctgaaa
     5341 ataatttgaa aaaaataagg gaagattacc aagatgtatt gatatgggca gagaaaaatt
     5401 atcgttttga ggaaatattg gaagccagca aatctggaaa atgccctaat gatctggatg
     5461 cattaagtcg acgatcgcta atactccaag aattgctgcg gcttgtttca tcaattagt
//
