LOCUS       sequence-362589-       11563 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To measure Syk intensity in response to ligand clustering using DNA 
            origami.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11563)
  AUTHORS   Kern N, Dong R, Douglas SM, Vale RD, Morrissey MA
  TITLE     Tight nanoscale clustering of Fcgamma receptors using DNA origami 
            promotes phagocytosis.
  JOURNAL   eLife. 2021 Jun 3;10. pii: 68311. doi: 10.7554/eLife.68311.
  PUBMED    34080973
REFERENCE   2  (bases 1 to 11563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-06-3"; volume: "10. pii: 68311. doi"; pages: " 
            10.7554/eLife.68311"
COMMENT     Sequence Label: NK66
FEATURES             Location/Qualifiers
     source          1..11563
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(34..50)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(35..51)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             141..374
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(399..417)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(400..417)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(707..726)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(940..959)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1059..1081
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1119..1137)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1205..1309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1310..1378,1379..2170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1528..1547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2341..2929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2830..2849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3083..3100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3170..3190)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3175..3504
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3355..3490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4638..4703
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4833..4853
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5278..5412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5315..5334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5369..5388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5580..6213
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6260..6385
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6882..7115
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7300..7344
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7629..7746
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7899..8306
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             10245..10946
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    join(11006..11563,1..31)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11059..11079)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11477..11488)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa
       61 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
      121 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
      181 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
      241 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
      301 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
      361 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
      421 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
      481 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
      541 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
      601 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
      661 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
      721 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
      781 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
      841 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
      901 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
      961 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     1021 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     1081 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     1141 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     1201 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     1261 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     1321 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     1381 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     1441 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     1501 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     1561 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     1621 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     1681 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     1741 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     1801 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     1861 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     1921 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     1981 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2041 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2101 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     2161 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     2221 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     2281 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     2341 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     2401 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     2461 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     2521 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     2581 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     2641 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     2701 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     2761 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     2821 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     2881 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     2941 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     3001 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     3061 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     3121 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     3181 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     3241 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     3301 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     3361 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3421 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     3481 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     3541 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     3601 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     3661 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     3721 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     3781 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     3841 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     3901 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     3961 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     4021 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     4081 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     4141 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     4201 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     4261 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     4321 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     4381 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     4441 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     4501 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     4561 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     4621 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     4681 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     4741 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     4801 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     4861 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     4921 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     4981 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     5041 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     5101 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     5161 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     5221 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     5281 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     5341 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     5401 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     5461 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     5521 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     5581 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     5641 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     5701 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     5761 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     5821 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     5881 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     5941 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     6001 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     6061 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6121 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6181 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     6241 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     6301 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     6361 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     6421 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     6481 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     6541 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     6601 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     6661 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     6721 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     6781 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     6841 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     6901 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     6961 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     7021 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     7081 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     7141 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     7201 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     7261 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     7321 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     7381 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     7441 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     7501 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     7561 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     7621 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     7681 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     7741 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     7801 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     7861 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     7921 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     7981 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     8041 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     8101 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     8161 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     8221 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     8281 tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt ctcacgcgta
     8341 tggcgggaag tgctgtggac agcgccaacc acctgaccta cttttttggc aacatcaccc
     8401 gggaagaggc tgaagactac ctggtccagg gaggcatgac cgatgggctc tacctgctac
     8461 gccagagccg caattacctg ggtggttttg ctttgtcggt ggctcacaac aggaaggcac
     8521 accactacac catcgagagg gaacttaatg gcacctacgc catctccggg ggcagggccc
     8581 atgccagccc agcagacctc tgccattacc actcccagga acctgatggc cttatctgcc
     8641 tccttaagaa gcccttcaac cggcccccgg gagtacagcc caagaccgga ccctttgagg
     8701 acctgaagga gaacctcatc agggaatatg tgaaacagac ctggaacctt cagggccagg
     8761 ctctggagca agccatcatc agccagaagc cccagctgga gaagctgatc gccaccacgg
     8821 cccatgagaa gatgccctgg ttccatggca acatctccag agatgaatca gagcagacgg
     8881 tcctcatagg gtcaaagacc aatggaaaat tcctgatcag ggccagagac aacagcggct
     8941 cctatgctct gtgcctgctg cacgaaggga aagtattgca ctaccgcatt gacagggaca
     9001 agaccgggaa gctctccatt cctgagggga agaagtttga caccctctgg cagctagtgg
     9061 aacattactc ttacaagcca gatgggctac taagagtcct cacggtacca tgccaaaaga
     9121 ttggtgcaca gatgggccac ccaggaagcc caaatgccca tcccgtgact tggtcaccgg
     9181 gtggaataat ctcaaggatc aaatcctact ccttcccaaa gcctggccac aaaaagcctg
     9241 ccccacccca agggagccgt ccagagagca ctgtgtcctt caacccctat gagccaacgg
     9301 gagggccctg gggcccagac agaggccttc agagagaagc cctgcccatg gacacagagg
     9361 tgtacgagag cccttatgct gaccctgaag agatccggcc caaagaggtc tacctggaca
     9421 ggagcctgct gaccctggag gacaatgaac tgggctccgg taacttcggg actgtgaaaa
     9481 agggatacta ccaaatgaaa aaagttgtga aaaccgtggc tgtgaaaatc ctgaagaacg
     9541 aggccaacga cccggctttg aaggacgagc tgctggcaga ggcgaacgtc atgcagcagc
     9601 tggacaaccc ctacattgtg cgcatgatcg gaatctgcga ggcggagtcc tggatgctgg
     9661 tgatggagat ggcggagctg gggccgctca acaagtacct gcagcagaac aggcacatta
     9721 aggataagaa catcatagag ctggttcacc aggtttccat ggggatgaag tatttggaag
     9781 agagcaactt tgtgcacaga gatctggctg cgcggaacgt gcttctggtc acacagcact
     9841 atgccaagat cagcgatttc ggtctttcca aagccctgcg tgctgatgaa aactactaca
     9901 aggcccagac ccacgggaag tggcccgtga agtggtacgc ccccgaatgc atcaactact
     9961 acaagttctc cagtaagagt gacgtctgga gcttcggagt cctgatgtgg gaagcgttct
    10021 cctatgggca gaagccctac agagggatga aagggagcga agtgaccgcc atgctggaga
    10081 aaggagagcg gatggggtgc cctgcaggat gcccgagaga gatgtacgac ctgatgaacc
    10141 tgtgctggac ttacgatgtg gagaacaggc caggattcac agctgtggaa ctgaggcttc
    10201 gcaattacta ctacgacgtg gttaacgcgg atccggtcgc caccatgagc gagctgatta
    10261 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
    10321 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
    10381 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
    10441 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
    10501 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
    10561 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
    10621 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
    10681 cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg
    10741 gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga
    10801 acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca
    10861 acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta
    10921 gcaaactggg gcacaagctt aattaagcgg ccgcgactct agagtcgacc tgcaggcatg
    10981 caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
    11041 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
    11101 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
    11161 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
    11221 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
    11281 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
    11341 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
    11401 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
    11461 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
    11521 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acg
//
