LOCUS       sequence-362590-       10627 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To test bead engulfment of macrophages..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10627)
  AUTHORS   Kern N, Dong R, Douglas SM, Vale RD, Morrissey MA
  TITLE     Tight nanoscale clustering of Fcgamma receptors using DNA origami 
            promotes phagocytosis.
  JOURNAL   eLife. 2021 Jun 3;10. pii: 68311. doi: 10.7554/eLife.68311.
  PUBMED    34080973
REFERENCE   2  (bases 1 to 10627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-06-3"; volume: "10. pii: 68311. doi"; pages: " 
            10.7554/eLife.68311"
COMMENT     Sequence Label: NK73
FEATURES             Location/Qualifiers
     source          1..10627
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    28..261
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             446..490
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    775..892
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1045..1452
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1563..1589
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1620..2162
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             join(2436..2438,2439..2441,2442..3155)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2436..2438,2439..2441,2442..3152)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2481..2502)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2742..2761)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3089..3110
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3216..3804
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3269..3289)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3687..3698)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3807..3823)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3808..3824)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3914..4147
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4172..4190)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4173..4190)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4480..4499)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4713..4732)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4832..4854
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4892..4910)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4978..5082
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5083..5151,5152..5943)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5301..5320)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6114..6702
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6603..6622
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6856..6873
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6943..6963)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6948..7277
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7128..7263
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8411..8476
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8606..8626
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9051..9185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9088..9107)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9142..9161
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9353..9986
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10033..10158
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
       61 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
      121 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
      181 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
      241 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
      301 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
      361 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
      421 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
      481 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
      541 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
      601 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
      661 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
      721 tcgattagtg aacggatctc gacggtatcg ccaaatggca gtattcatcc acaattttaa
      781 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
      841 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
      901 ttacagggac agcagagatc cagtttggat cgataagctt gatatcgaat tcctgcagcc
      961 ccgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
     1021 tgtaggtttg gcaagctagc tgcagtaacg ccattttgca aggcatggaa aaataccaaa
     1081 ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac gttgggccaa
     1141 acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca gatggtcacc
     1201 gcagtttcgg ccccggcccg aggccaagaa cagatggtcc ccagatatgg cccaaccctc
     1261 agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg aaatgaccct
     1321 gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc gcttctgctt
     1381 cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct ccgacagact
     1441 gagtcgcccg ggggggatct ggagctctcg agaattctca cgcggccgcc accatgcagt
     1501 cgggcactca ctggagagtt ctgggcctct gcctcttatc agttggcgtt tgggggcaag
     1561 attacccata cgacgtccca gactacgctc tgcccgagac cggcggcggc ggcggcggcg
     1621 acaaagactg cgaaatgaag cgcaccaccc tggatagccc tctgggcaag ctggaactgt
     1681 ctgggtgcga acagggcctg caccgtatca tcttcctggg caaaggaaca tctgccgccg
     1741 acgccgtgga agtgcctgcc ccagccgccg tgctgggcgg accagagcca ctgatgcagg
     1801 ccaccgcctg gctcaacgcc tactttcacc agcctgaggc catcgaggag ttccctgtgc
     1861 cagccctgca ccacccagtg ttccagcagg agagctttac ccgccaggtg ctgtggaaac
     1921 tgctgaaagt ggtgaagttc ggagaggtca tcagctacag ccacctggcc gccctggccg
     1981 gcaatcccgc cgccaccgcc gccgtgaaaa ccgccctgag cggaaatccc gtgcccattc
     2041 tgatcccctg ccaccgggtg gtgcagggcg acctggacgt ggggggctac gagggcgggc
     2101 tcgccgtgaa agagtggctg ctggcccacg agggccacag actgggcaag cctgggctgg
     2161 gtggcggaag cggaggtagt ggtggaagcc ccccagacca cattccttgg attacagctg
     2221 tacttccaac agttattata tgtgtgatgg ttttctgtct aattctatgg aaatggaaga
     2281 agaagaagcg gcctcggcga ctcaagatcc aggtccgaaa ggcagctata gccagccgtg
     2341 agaaagcaga tgctgtctac acgggcctga acacccggag ccaggagaca tatgagactc
     2401 tgaagcatga gaaaccaccc cagggatccg gaagtatggt gagcaagggc gaggagctgt
     2461 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     2521 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     2581 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     2641 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2701 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2761 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2821 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2881 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     2941 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     3001 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     3061 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     3121 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcgactct agagtcgacc
     3181 tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt
     3241 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3301 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3361 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3421 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3481 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3541 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3601 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     3661 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3721 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3781 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat taattcgagc
     3841 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     3901 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     3961 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4021 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4081 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4141 tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta
     4201 tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta
     4261 attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca
     4321 gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa
     4381 tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg
     4441 tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg
     4501 atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg
     4561 tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg
     4621 cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc
     4681 ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     4741 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     4801 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     4861 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     4921 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4981 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     5041 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     5101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     5161 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     5221 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     5281 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5341 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5401 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5461 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5521 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5581 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5641 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5701 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5761 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5821 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5881 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5941 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6001 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     6061 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     6121 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     6181 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     6241 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6301 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6361 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6421 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6481 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6541 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6601 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6661 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6721 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6781 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6841 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6901 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag
     6961 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     7021 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7081 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     7141 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7201 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7261 gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata
     7321 ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata
     7381 ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact
     7441 ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc
     7501 tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa
     7561 tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg
     7621 aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc
     7681 gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg
     7741 acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact
     7801 ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata
     7861 tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa
     7921 tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca
     7981 ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac
     8041 ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc
     8101 cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct
     8161 cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa
     8221 atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta
     8281 tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt
     8341 tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta
     8401 aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat
     8461 tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc
     8521 ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac
     8581 tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga
     8641 ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc
     8701 ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga
     8761 aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt
     8821 tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac
     8881 ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac
     8941 tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     9001 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     9061 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     9121 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     9181 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta
     9241 ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat
     9301 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg
     9361 ctaattcact cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc
     9421 tacttccctg attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt
     9481 ggatggtgct acaagctagt accagttgag ccagataagg tagaagaggc caataaagga
     9541 gagaacacca gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa
     9601 gtgttagagt ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat
     9661 ccggagtact tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac
     9721 tttccaggga ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata
     9781 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     9841 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     9901 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     9961 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
    10021 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
    10081 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
    10141 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
    10201 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
    10261 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
    10321 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
    10381 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
    10441 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
    10501 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
    10561 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
    10621 gagtggt
//
