LOCUS       sequence-362591-       11208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To measure Syk intensity in response to ligand clustering using DNA 
            origami.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11208)
  AUTHORS   Kern N, Dong R, Douglas SM, Vale RD, Morrissey MA
  TITLE     Tight nanoscale clustering of Fcgamma receptors using DNA origami 
            promotes phagocytosis.
  JOURNAL   eLife. 2021 Jun 3;10. pii: 68311. doi: 10.7554/eLife.68311.
  PUBMED    34080973
REFERENCE   2  (bases 1 to 11208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-06-3"; volume: "10. pii: 68311. doi"; pages: " 
            10.7554/eLife.68311"
COMMENT     Sequence Label: NK83
FEATURES             Location/Qualifiers
     source          1..11208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(23..39)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(24..40)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             130..363
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(388..406)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(389..406)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(696..715)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(929..948)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1048..1070
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1108..1126)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1194..1298
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1299..1367,1368..2159)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1517..1536)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2330..2918
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2819..2838
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3072..3089
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(3159..3179)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3164..3493
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3344..3479
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          4627..4692
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4822..4842
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5267..5401
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5304..5323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5358..5377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             5569..6202
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6249..6374
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6871..7104
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7289..7333
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7618..7735
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7791..9002
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     8839..8858
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      9049..9058
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9066..9773
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      9321..9330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(10640..11208,1..20)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10693..10713)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11111..11122)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat tcgagctcgg
       61 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
      121 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
      181 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
      241 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
      301 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
      361 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
      421 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
      481 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
      541 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
      601 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
      661 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
      721 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
      781 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
      841 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
      901 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
      961 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     1021 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     1081 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     1141 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     1201 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     1261 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     1321 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     1381 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     1441 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1501 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1561 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1621 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1681 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1741 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1801 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1861 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1921 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1981 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     2041 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     2101 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     2161 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     2221 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     2281 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     2341 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     2401 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     2461 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2521 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2581 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2641 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2701 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2761 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2821 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2881 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2941 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     3001 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     3061 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     3121 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     3181 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3241 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3301 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3361 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     3421 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     3481 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
     3541 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
     3601 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
     3661 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
     3721 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
     3781 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
     3841 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
     3901 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
     3961 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
     4021 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
     4081 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
     4141 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
     4201 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
     4261 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
     4321 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
     4381 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
     4441 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
     4501 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
     4561 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
     4621 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
     4681 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
     4741 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
     4801 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
     4861 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
     4921 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
     4981 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
     5041 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
     5101 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
     5161 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     5221 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     5281 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5341 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     5401 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
     5461 cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc
     5521 attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg gaagggctaa
     5581 ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
     5641 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat
     5701 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga
     5761 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt
     5821 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg
     5881 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc
     5941 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag
     6001 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     6061 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     6121 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     6181 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     6241 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     6301 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     6361 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     6421 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     6481 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     6541 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     6601 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     6661 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc
     6721 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     6781 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     6841 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     6901 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     6961 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     7021 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     7081 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     7141 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     7201 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     7261 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     7321 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     7381 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     7441 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     7501 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     7561 cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca tccacaattt
     7621 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     7681 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     7741 ttattacagg gacagcagag atccagtttg gatcgatgaa ttcgatatcg ggcctccgcg
     7801 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     7861 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     7921 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     7981 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     8041 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     8101 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     8161 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     8221 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     8281 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     8341 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     8401 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     8461 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     8521 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     8581 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     8641 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     8701 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     8761 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     8821 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     8881 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     8941 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     9001 acgaagcttg ggctgcaggt cgactctaga gcatccccgg gtaccggtgc caccatggaa
     9061 cgcgtatggt gagcaagggc gaggaggata acatggcctc tctcccagcg acacatgagt
     9121 tacacatctt tggctccatc aacggtgtgg actttgacat ggtgggtcag ggcaccggca
     9181 atccaaatga tggttatgag gagttaaacc tgaagtccac caagggtgac ctccagttct
     9241 ccccctggat tctggtccct catatcgggt atggcttcca tcagtacctg ccctaccctg
     9301 acgggatgtc gcctttccag gccgccatgg tagatggctc cggataccaa gtccatcgca
     9361 caatgcagtt tgaagatggt gcctccctta ctgttaacta ccgctacacc tacgagggaa
     9421 gccacatcaa aggagaggcc caggtgaagg ggactggttt ccctgctgac ggtcctgtga
     9481 tgaccaactc gctgaccgct gcggactggt gcaggtcgaa gaagacttac cccaacgaca
     9541 aaaccatcat cagtaccttt aagtggagtt acaccactgg aaatggcaag cgctaccgga
     9601 gcactgcgcg gaccacctac acctttgcca agccaatggc ggctaactat ctgaagaacc
     9661 agccgatgta cgtgttccgt aagacggagc tcaagcactc caagaccgag ctcaacttca
     9721 aggagtggca aaaggccttt accgatgtga tgggcatgga cgagctgtac aagggaggcg
     9781 gatccggagg cggaggtgcg ggaagtgctg tggacagcgc caaccacctg acctactttt
     9841 ttggcaacat cacccgggaa gaggctgaag actacctggt ccagggaggc atgaccgatg
     9901 ggctctacct gctacgccag agccgcaatt acctgggtgg ttttgctttg tcggtggctc
     9961 acaacaggaa ggcacaccac tacaccatcg agagggaact taatggcacc tacgccatct
    10021 ccgggggcag ggcccatgcc agcccagcag acctctgcca ttaccactcc caggaacctg
    10081 atggccttat ctgcctcctt aagaagccct tcaaccggcc cccgggagta cagcccaaga
    10141 ccggaccctt tgaggacctg aaggagaacc tcatcaggga atatgtgaaa cagacctgga
    10201 accttcaggg ccaggctctg gagcaagcca tcatcagcca gaagccccag ctggagaagc
    10261 tgatcgccac cacggcccat gagaagatgc cctggttcca tggcaacatc tccagagatg
    10321 aatcagagca gacggtcctc atagggtcaa agaccaatgg aaaattcctg atcagggcca
    10381 gagacaacag cggctcctat gctctgtgcc tgctgcacga agggaaagta ttgcactacc
    10441 gcattgacag ggacaagacc gggaagctct ccattcctga ggggaagaag tttgacaccc
    10501 tctggcagct agtggaacat tactcttaca agccagatgg gctactaaga gtcctcacgg
    10561 taccatgcca aaagatttaa gcggccgcga ctctagagtc gacctgcagg catgcaagct
    10621 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
    10681 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
    10741 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
    10801 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
    10861 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
    10921 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    10981 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
    11041 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    11101 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
    11161 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctc
//
