LOCUS       sequence-362592-       10597 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To minimize dimerization between FcRg transmembrane domains, as this
            cysteine forms a disulfide bridge between y chains. To manipulate 
            ligand spacing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10597)
  AUTHORS   Kern N, Dong R, Douglas SM, Vale RD, Morrissey MA
  TITLE     Tight nanoscale clustering of Fcgamma receptors using DNA origami 
            promotes phagocytosis.
  JOURNAL   eLife. 2021 Jun 3;10. pii: 68311. doi: 10.7554/eLife.68311.
  PUBMED    34080973
REFERENCE   2  (bases 1 to 10597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-06-3"; volume: "10. pii: 68311. doi"; pages: " 
            10.7554/eLife.68311"
COMMENT     Sequence Label: NK85
FEATURES             Location/Qualifiers
     source          1..10597
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1201..1266
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1396..1416
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1841..1975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1878..1897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1932..1951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2143..2776
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2823..2948
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3445..3678
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3863..3907
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4192..4309
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4462..4869
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             4980..5006
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5037..5579
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             join(5823..5825,5826..5828,5829..6542)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5823..5825,5826..5828,5829..6539)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5868..5889)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6129..6148)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6476..6497
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    6603..7191
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6656..6676)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7074..7085)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7194..7210)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7195..7211)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7301..7534
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7559..7577)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7560..7577)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7867..7886)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8100..8119)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8219..8241
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8279..8297)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8365..8469
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8470..8538,8539..9330)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8688..8707)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9501..10089
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9990..10009
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10243..10260
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10330..10350)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10335..10597,1..67)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10515..10597,1..53)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
       61 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
      121 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
      181 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
      241 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
      301 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
      361 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
      421 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
      481 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
      541 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
      601 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
      661 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
      721 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
      781 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
      841 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
      901 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
      961 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     1021 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     1081 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     1141 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     1201 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     1261 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     1321 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     1381 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     1441 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     1501 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     1561 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     1621 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     1681 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     1741 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     1801 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     1861 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     1921 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     1981 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     2041 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     2101 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     2161 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     2221 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     2281 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     2341 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     2401 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     2461 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     2521 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     2581 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2641 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2701 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2761 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     2821 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     2881 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     2941 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     3001 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     3061 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     3121 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     3181 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     3241 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     3301 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     3361 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     3421 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     3481 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     3541 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     3601 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     3661 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     3721 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     3781 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     3841 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     3901 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     3961 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     4021 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     4081 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     4141 attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag
     4201 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4261 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     4321 cagggacagc agagatccag tttggatcga taagcttgat atcgaattcc tgcagccccg
     4381 ataaaataaa agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt
     4441 aggtttggca agctagctgc agtaacgcca ttttgcaagg catggaaaaa taccaaacca
     4501 agaatagaga agttcagatc aagggcgggt acatgaaaat agctaacgtt gggccaaaca
     4561 ggatatctgc ggtgagcagt ttcggccccg gcccggggcc aagaacagat ggtcaccgca
     4621 gtttcggccc cggcccgagg ccaagaacag atggtcccca gatatggccc aaccctcagc
     4681 agtttcttaa gacccatcag atgtttccag gctcccccaa ggacctgaaa tgaccctgcg
     4741 ccttatttga attaaccaat cagcctgctt ctcgcttctg ttcgcgcgct tctgcttccc
     4801 gagctctata aaagagctca caacccctca ctcggcgcgc cagtcctccg acagactgag
     4861 tcgcccgggg gggatctgga gctctcgaga attctcacgc ggccgccacc atgcagtcgg
     4921 gcactcactg gagagttctg ggcctctgcc tcttatcagt tggcgtttgg gggcaagatt
     4981 acccatacga cgtcccagac tacgctctgc ccgagaccgg cggcggcggc ggcggcgaca
     5041 aagactgcga aatgaagcgc accaccctgg atagccctct gggcaagctg gaactgtctg
     5101 ggtgcgaaca gggcctgcac cgtatcatct tcctgggcaa aggaacatct gccgccgacg
     5161 ccgtggaagt gcctgcccca gccgccgtgc tgggcggacc agagccactg atgcaggcca
     5221 ccgcctggct caacgcctac tttcaccagc ctgaggccat cgaggagttc cctgtgccag
     5281 ccctgcacca cccagtgttc cagcaggaga gctttacccg ccaggtgctg tggaaactgc
     5341 tgaaagtggt gaagttcgga gaggtcatca gctacagcca cctggccgcc ctggccggca
     5401 atcccgccgc caccgccgcc gtgaaaaccg ccctgagcgg aaatcccgtg cccattctga
     5461 tcccctgcca ccgggtggtg cagggcgacc tggacgtggg gggctacgag ggcgggctcg
     5521 ccgtgaaaga gtggctgctg gcccacgagg gccacagact gggcaagcct gggctgggtg
     5581 gcggaagcgg aggtagtggt ggaagcctgg gagagccgca gctcgcttat atcctggatg
     5641 ctgtcctgtt tttgtatggt attgtcctta ccctactcta ctgtcgactc aagatccagg
     5701 tccgaaaggc agctatagcc agccgtgaga aagcagatgc tgtctacacg ggcctgaaca
     5761 cccggagcca ggagacatat gagactctga agcatgagaa accaccccag ggatccggaa
     5821 gtatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5881 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5941 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     6001 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     6061 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     6121 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     6181 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     6241 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     6301 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     6361 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     6421 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     6481 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     6541 aaagcggccg cgactctaga gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg
     6601 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6661 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6721 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6781 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6841 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6901 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6961 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     7021 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     7081 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     7141 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc
     7201 gtcgacctcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca
     7261 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     7321 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     7381 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7441 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7501 agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc
     7561 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
     7621 gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc
     7681 tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt
     7741 ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta
     7801 gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg
     7861 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca
     7921 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg
     7981 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac
     8041 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt
     8101 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
     8161 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
     8221 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
     8281 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     8341 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     8401 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     8461 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     8521 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     8581 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     8641 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     8701 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     8761 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     8821 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     8881 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8941 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     9001 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     9061 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     9121 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     9181 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     9241 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     9301 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     9361 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     9421 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     9481 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     9541 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     9601 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     9661 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     9721 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     9781 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     9841 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9901 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9961 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
    10021 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
    10081 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
    10141 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
    10201 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
    10261 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
    10321 tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
    10381 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
    10441 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
    10501 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
    10561 gctgactaat tttttttatt tatgcagagg ccgaggc
//
