LOCUS       sequence-362593-       10735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To manipulate ligand spacing..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Kern N, Dong R, Douglas SM, Vale RD, Morrissey MA
  TITLE     Tight nanoscale clustering of Fcgamma receptors using DNA origami 
            promotes phagocytosis.
  JOURNAL   eLife. 2021 Jun 3;10. pii: 68311. doi: 10.7554/eLife.68311.
  PUBMED    34080973
REFERENCE   2  (bases 1 to 10735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2021-06-3"; volume: "10. pii: 68311. doi"; pages: " 
            10.7554/eLife.68311"
COMMENT     Sequence Label: NK88
FEATURES             Location/Qualifiers
     source          1..10735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    4..237
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             422..466
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    751..868
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1021..1428
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1539..1565
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1596..2138
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="DKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVEV
                     PAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKV
                     VKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLAV
                     KEWLLAHEGHRLGKPGLG"
     CDS             join(2520..2522,2523..2525,2526..3239)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2520..2522,2523..2525,2526..3236)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2565..2586)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2826..2845)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3173..3194
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3300..3888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3353..3373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3771..3782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3891..3907)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3892..3908)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3998..4231
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4256..4274)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4257..4274)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4564..4583)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4797..4816)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4916..4938
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4976..4994)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5062..5166
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5167..5235,5236..6027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5385..5404)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6198..6786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6687..6706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6940..6957
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7027..7047)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7032..7361
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7212..7347
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8495..8560
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8690..8710
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9135..9269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9172..9191)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9226..9245
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9437..10070
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10117..10242
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
       61 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
      121 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
      181 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
      241 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
      301 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
      361 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
      421 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
      481 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
      541 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
      601 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
      661 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
      721 gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
      781 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
      841 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
      901 ttggatcgat aagcttgata tcgaattcct gcagccccga taaaataaaa gattttattt
      961 agtctccaga aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctgca
     1021 gtaacgccat tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca
     1081 agggcgggta catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt
     1141 tcggccccgg cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc
     1201 caagaacaga tggtccccag atatggccca accctcagca gtttcttaag acccatcaga
     1261 tgtttccagg ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc
     1321 agcctgcttc tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
     1381 aacccctcac tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag
     1441 ctctcgagaa ttctcacgcg gccgccacca tgcagtcggg cactcactgg agagttctgg
     1501 gcctctgcct cttatcagtt ggcgtttggg ggcaagatta cccatacgac gtcccagact
     1561 acgctctgcc cgagaccggc ggcggcggcg gcggcgacaa agactgcgaa atgaagcgca
     1621 ccaccctgga tagccctctg ggcaagctgg aactgtctgg gtgcgaacag ggcctgcacc
     1681 gtatcatctt cctgggcaaa ggaacatctg ccgccgacgc cgtggaagtg cctgccccag
     1741 ccgccgtgct gggcggacca gagccactga tgcaggccac cgcctggctc aacgcctact
     1801 ttcaccagcc tgaggccatc gaggagttcc ctgtgccagc cctgcaccac ccagtgttcc
     1861 agcaggagag ctttacccgc caggtgctgt ggaaactgct gaaagtggtg aagttcggag
     1921 aggtcatcag ctacagccac ctggccgccc tggccggcaa tcccgccgcc accgccgccg
     1981 tgaaaaccgc cctgagcgga aatcccgtgc ccattctgat cccctgccac cgggtggtgc
     2041 agggcgacct ggacgtgggg ggctacgagg gcgggctcgc cgtgaaagag tggctgctgg
     2101 cccacgaggg ccacagactg ggcaagcctg ggctgggtgg cggaagcgga ggtagtggtg
     2161 gaagcggtcc ccagtcatca gctcctgtct ggtttcacat cctgttttat ctgtcagtgg
     2221 gaataatgtt ttcgttgaac acggttctct atgtgaaaat acacaggctg cagagagaga
     2281 agaaatacaa cttagaagtc cctttggttt ctgagcaggg aaagaaagca aattcctttc
     2341 agcaagttag aagcgatggc gtgtatgaag aagtaacagc cactgcgagc cagaccacac
     2401 caaaagaagc gcccgatgga cctcgaagct cagtgggtga ctgtggaccc gagcagcctg
     2461 aaccccttcc tcccagtgac agtactgggg cacaaacttc ccaaagtgga tccggaagta
     2521 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2581 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2641 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     2701 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     2761 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     2821 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     2881 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     2941 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3001 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3061 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3121 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3181 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa
     3241 gcggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata
     3301 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3361 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3421 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3481 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3541 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3601 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3661 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3721 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3781 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3841 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     3901 gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta caaggcagct
     3961 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     4021 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     4081 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     4141 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     4201 cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat
     4261 agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt
     4321 ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat
     4381 catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg
     4441 gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc
     4501 agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg
     4561 gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga
     4621 gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca
     4681 tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg
     4741 gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca
     4801 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4861 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4921 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4981 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     5041 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     5101 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     5161 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     5221 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     5281 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5341 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5401 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5461 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5521 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5581 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5641 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5701 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5761 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5821 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5881 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5941 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     6001 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     6061 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6121 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6181 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6241 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6301 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     6361 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6421 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6481 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6541 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6601 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6661 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6721 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6781 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6841 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6901 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6961 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     7021 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     7081 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     7141 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     7201 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     7261 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     7321 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag
     7381 gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
     7441 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
     7501 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
     7561 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
     7621 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
     7681 taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt
     7741 ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg
     7801 gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc
     7861 gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc
     7921 tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata
     7981 tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc
     8041 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
     8101 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
     8161 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
     8221 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
     8281 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
     8341 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
     8401 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
     8461 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
     8521 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
     8581 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
     8641 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
     8701 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
     8761 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
     8821 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
     8881 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
     8941 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
     9001 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     9061 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     9121 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9181 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9241 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     9301 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     9361 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     9421 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     9481 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     9541 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     9601 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     9661 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     9721 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     9781 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     9841 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     9901 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9961 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
    10021 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
    10081 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
    10141 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
    10201 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
    10261 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
    10321 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
    10381 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
    10441 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
    10501 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
    10561 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
    10621 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
    10681 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtggg
//
