LOCUS       sequence-362670-       10434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon usage 
            anCas(PDCA) with an N-terminal NLS, C-terminal bi-partite NLS, and 
            P2A-eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille 
            LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S,
            Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias 
            A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu 
            L, Perez-Jimenez R
  TITLE     Evolution of CRISPR-associated endonucleases as inferred from 
            resurrected proteins.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y.
            Epub 2023 Jan 2.
  PUBMED    36593295
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01265-y"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "77-90"
COMMENT     Sequence Label: pCMV-T7-NLS(SV40)-anCas(PDCA)-BPNLS(SV40)-P2A-...
FEATURES             Location/Qualifiers
     source          1..10434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(149..168)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        340..719
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        720..923
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     874..894
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     968..987
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        968..986
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1037..1057
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5213..5233
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5252..5308
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5309..5311,5312..5314,5315..6028)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5309..5311,5312..5314,5315..6025)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5615..5634)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5962..5983
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6062..6082
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6130..6147)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6136..6360
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6406..6834
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6493..6512)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6703..6724
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6843..6863)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6848..7177
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7028..7163
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7090..7109
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7244..8038
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7298..7317)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7908..7927
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8212..8333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8249..8268)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8303..8322
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8382..8398)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8382..8398)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8395..8417)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8406..8422
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8430..8436,8437..8454,8455..8460))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8475..8496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8613..8630)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8784..9372)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8864..8883)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9543..10334,10335..10403))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10166..10185
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10404..10434,1..74))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
       61 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
      121 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
      181 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
      241 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
      301 ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt
      361 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
      421 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      481 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      541 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      601 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      661 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      721 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      781 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      841 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      901 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
      961 tcgaaattaa tacgactcac tatagggaga cccaagctgg ctagcgttta aacttaagct
     1021 tggtaccgcc accatgccca agaaaaagcg gaaggtgccc aagatggaca agaaatactc
     1081 tatcggtctg gatatcggca ccaacagcgt gggctgggcc gtgatcacag acgattacaa
     1141 agtgcctagc aaaaagttca aggtgctggg aaacaccgat agacacagca tcaagaagaa
     1201 tttgatcggc gccctgctgt tcgatagcgg cgaaaccgcc gaagccacca gactgaagag
     1261 gaccgcccgg agaagataca ccagaagaaa gaaccgaatt tgttacctgc aggagatctt
     1321 ttctaatgag atggccaagg tggacgactc ttttttccac agactggaag agtccttcct
     1381 ggtggaggag gacaagaagc acgagcggca ccccatcttc ggaaacatcg tggacgaggt
     1441 ggcctatcac gagaagtatc ccaccatcta ccacctgaga aagaagctgg ccgactccac
     1501 cgacaaagca gacctgagac tgatctatct ggccctggct cacatgatca aattccgcgg
     1561 ccacttcctg atcgagggag atctcaaccc tgataacagc gatgtggaca agctgttcat
     1621 ccagctggtg cagacctaca accagctgtt cgaggagaat cccatcaacg cctctggagt
     1681 ggacgccaag gccatcctga gcgccagact gagcaagtcc cggagactgg agaacctgat
     1741 cgcccagctg cctggcgaga agaaaaacgg cctgtttgga aatctgatcg cactgtccct
     1801 gggactgaca cctaatttca agagcaactt cgacctggcc gaggatgcta aactgcagct
     1861 gagcaaagac acctacgacg atgacctgga caacctgctg gcccaaatcg gcgaccagta
     1921 cgccgacctg ttcctggccg ccaagaacct gagcgacgcc attctgctca gcgatatcct
     1981 gagagtgaac agcgagatca caaaggcacc tctgagcgct agcatgatca agcgatacga
     2041 tgagcaccac caggacctga ccctgctcaa ggctctggtg cggcagcagc tgccagaaaa
     2101 atacaaggag atctttttcg accagagcaa gaatggatac gccggctaca tcgacggcgg
     2161 cgcctctcag gaggagttct acaagttcat taagcctatc ctggagaaga tggacggcac
     2221 agaggaactc ctggccaaac tcaaccggga ggacctcctg agaaaacagc ggacattcga
     2281 caatggcagc atcccccacc agatccacct gggcgagctg cacgccatcc tgaggaggca
     2341 ggaggacttc tacccattcc tgaaggacaa cagagagaag atcgagaaga tcctgacctt
     2401 cagaattcct tactacgtgg gacctctggc cagaggcaac tccagattcg cctggatgac
     2461 ccggaagagc gaggaaacca tcaccccttg gaacttcgag gaggtggtgg ataagggcgc
     2521 ttctgcccaa agcttcatcg aaagaatgac aaacttcgac aagaacctgc caaacgaaaa
     2581 ggtgctgcct aaacactctc tgctgtacga gtactttacc gtgtacaacg agctgaccaa
     2641 ggtgaagtac gttacagagg gcatgagaaa acctgccttc ctaagcggcg agcagaaaaa
     2701 ggctatcgtg gatctgctgt ttaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     2761 ggactacttc aagaagatcg agtgctttga ctctgtggaa atatctggcg tggaggaccg
     2821 gttcaacgca agcctgggaa cataccacga cctgctgaag atcatcaaag acaaggattt
     2881 cctggacaat gaagaaaacg aggatatcct ggaagatatt gtgctgacac tgacactgtt
     2941 cgaggacaga gagatgatcg aagaaagact gaaaacgtac gcccatctgt tcgatgataa
     3001 agtgatgaag cagctgaaaa gacggcacta caccggctgg ggcagactgt cccggaagct
     3061 gatcaacggc atccgtgaca agcagagcgg caagacaatc ctggactttc tcaagagcga
     3121 cggcttcgcc aaccggaact ttatgcagct gatccacgac gactccctga cattcaagga
     3181 ggatatccaa aaagcccagg tgtccggcca gggcgattcc ctccatgagc agatagctaa
     3241 cctggctggc agccccgcca tcaagaaagg catcctccag accgtcaagg tggtggatga
     3301 gctggtgaag gtgatgggca gacataagcc cgagaatatc gttattgaga tggctagaga
     3361 aaaccagaca acccagaaag gccagaagaa cagcagagag cggatgaaaa gaatcgagga
     3421 aggcatcaag gaactgggca gccagatcct caaggaacac cctgtggaaa acacccagct
     3481 acaaaatgag aagctgtacc tgtattacct gcaaaacggc cgggacatgt acgtggacca
     3541 ggagctcgac atcaaccggc tgagcgatta tgacgtggac cacatcgtgc cccagtcatt
     3601 catcaaagat gacagcatcg acaacaaggt gctgacccgg agcgacaaga atagaggcaa
     3661 gtctgacaat gtgcccagcg aagaagtggt gaaaaagatg aagaattatt ggcggcagct
     3721 gctgaacgcc aagctgatta cacagcggaa gttcgataac ctgacaaagg ccgagagagg
     3781 aggcctgtct gaactggaca aggccggctt catcaagcgg cagctggtgg agaccagaca
     3841 gatcacaaag cacgtggctc aaatcctgga cagcagaatg aataccaagt acgacgaaaa
     3901 cgacaagcta atccgggagg tgaaggtgat caccctgaag tcaaagctgg tttccgactt
     3961 cagaaaggat ttccagttct acaaggtgcg ggaaatcaac aactaccacc acgcccacga
     4021 cgcctatctg aacgctgtcg tcggaaccgc cctcatcaag aagtacccca agctggaatc
     4081 tgagttcgtg tacggcgatt acaaggttta cgacgtgcgc aagatgatcg ccaagagtga
     4141 acaagaaatc ggaaaagcga cagccaagag attcttctac agcaatatca tgaacttctt
     4201 caagaccgag atcaccttag ccaatggaga aatcagaaag cgccccctga tcgaaactaa
     4261 cggcgagaca ggcgagatcg tctgggacaa aggcagagat tttgccacag tcagaaaggt
     4321 gctgagcatg ccacaagtga atatcgtgaa gaagaccgag gtgcagaccg gcggatttag
     4381 caaggaaagc atcctgccta agagaaacag cgataagctg attgcccgga aaaaggactg
     4441 ggatcctaaa aaatacgggg gctttgattc ccctaccgtg gcctatagcg tgctggtggt
     4501 ggccaaggtc gaaaagggca aggccaagaa gctgaaatcc gtgaaagaat tggttggaat
     4561 taccatcatg gaacgcagca gcttcgagaa gaaccctata gacttcctgg aagctaaagg
     4621 gtacaaggac gtacagaaag acctaatcat caagctgcca aagtacagcc tgttcgagct
     4681 tgagaacggc agaagacgga tgctggccag cgccggtgag ttgcagaaag gtaacgaaat
     4741 ggtgctgcct gccaaactgg tgacattcct ttaccatgcc agccacatcg agaagagcaa
     4801 gggctctcct gagaacaacg agcaaaagca ggcctacatc gagcaacaca agcacgacct
     4861 ggacgagatc ctggaataca tcagcgaatt cagcaagaga tacatcctgg ccgataagaa
     4921 tctatctaag gtgaaatcac tgtttaacaa gcacgaggat agcagcatca gcgagctggc
     4981 aagcagcatc atcaacctgt tcaccctgac aagcctgggc gcccctgctg ctttcaagtt
     5041 cctggatacc accatcgaca gaaaaagata caccagcacc aaggaagtgc tggatgccac
     5101 cctgatccac cagtcaataa caggcctgta cgagacacgg atcgacctct ctcagctggg
     5161 cggagatcac tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa
     5221 gaagaggaaa gtcggaggcg gtggaagcgg cgcaacaaac ttctctctgc tgaaacaagc
     5281 cggagatgtc gaagagaatc ctggaccgat ggtgagcaag ggcgaggagc tgttcaccgg
     5341 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     5401 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     5461 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     5521 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     5581 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     5641 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     5701 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     5761 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     5821 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     5881 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     5941 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     6001 tctcggcatg gacgagctgt acaagtgaca tcatcatcac cacgcctctg gaacaggcgg
     6061 acctaaaaag aagaggaagg tttaagcggc cgctcgagtc tagagggccc gtttaaaccc
     6121 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6181 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6241 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6301 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6361 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6421 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6481 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6541 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6601 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6661 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6721 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6781 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6841 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6901 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6961 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     7021 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7081 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     7141 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     7201 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     7261 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     7321 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     7381 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
     7441 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     7501 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     7561 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     7621 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     7681 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     7741 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
     7801 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     7861 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     7921 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     7981 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     8041 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     8101 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     8161 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt
     8221 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8281 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8341 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg
     8401 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8461 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8521 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     8581 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     8641 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     8701 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     8761 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     8821 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     8881 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     8941 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     9001 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     9061 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     9121 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     9181 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     9241 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     9301 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     9361 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     9421 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     9481 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     9541 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     9601 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     9661 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     9721 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     9781 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     9841 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     9901 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     9961 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
    10021 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
    10081 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
    10141 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
    10201 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
    10261 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
    10321 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
    10381 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catt
//
