LOCUS       sequence-362671-       10389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon usage 
            anCas(BCA) with an N-terminal NLS, C-terminal bi-partite NLS, and 
            P2A-eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10389)
  AUTHORS   Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille 
            LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S,
            Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias 
            A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu 
            L, Perez-Jimenez R
  TITLE     Evolution of CRISPR-associated endonucleases as inferred from 
            resurrected proteins.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y.
            Epub 2023 Jan 2.
  PUBMED    36593295
REFERENCE   2  (bases 1 to 10389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01265-y"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "77-90"
COMMENT     Sequence Label: pCMV-T7-NLS(SV40)-anCas(BCA)-BPNLS(SV40)-P2A-E...
FEATURES             Location/Qualifiers
     source          1..10389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..34
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             169..963
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(223..242)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     833..852
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1137..1258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1174..1193)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1228..1247
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1307..1323)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1307..1323)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1320..1342)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1331..1347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1355..1361,1362..1379,1380..1385))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1400..1421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1538..1555)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1709..2297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1789..1808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2468..3259,3260..3328))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3091..3110
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3329..3433)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3508..3527)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3699..4078
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4079..4282
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4233..4253
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4327..4346
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4327..4345
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4374..4394
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8466..8486
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8505..8561
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(8562..8564,8565..8567,8568..9281)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8562..8564,8565..8567,8568..9278)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8868..8887)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9215..9236
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             9315..9335
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(9444..9461)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9450..9674
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9720..10148
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9807..9826)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10017..10038
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10157..10177)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10162..10389,1..102)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(10342..10389,1..88)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
       61 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
      121 atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa
      181 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg
      241 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc
      301 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca
      361 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc
      421 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca
      481 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat
      541 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca
      601 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg
      661 ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc
      721 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct
      781 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct
      841 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac
      901 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc
      961 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag
     1021 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     1081 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     1141 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     1201 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     1261 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     1321 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     1381 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1441 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1501 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1561 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1621 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1681 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1741 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1801 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1861 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1921 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1981 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     2041 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     2101 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     2161 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     2221 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     2281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     2341 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     2401 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2461 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2521 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2581 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2641 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2701 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2761 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2821 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2881 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2941 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     3001 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     3061 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     3121 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     3181 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     3241 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     3301 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     3361 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     3421 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     3481 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     3541 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     3601 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     3661 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     3721 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     3781 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     3841 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     3901 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     3961 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     4021 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     4081 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     4141 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     4201 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     4261 ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt actggcttat
     4321 cgaaattaat acgactcact atagggagac ccaagctggc tagcgccacc atgcccaaga
     4381 aaaagcggaa ggtgcccaag atgaagaagg actattctat cggactggac atcggcacaa
     4441 acagcgtggg ctgggccgtg atcacagacg actacaaggt cgtgcgcaag aaaatgaagg
     4501 tgctgggcaa caccgataaa aagagcatca agaaaaacct gtggggagtg agactgttcg
     4561 actctggcga aaccgccgag gatacaagac tgaagcgaac caccagacgg agatacacca
     4621 gaagaagaaa ccgcatctgc tacctgcagg aaatcttcca ggaagagatg aacaaggtcg
     4681 acgacagctt cttccaccgc ctggatgaaa gcttcctggt gcccgaggat aaaaagtacg
     4741 acagacaccc tatctttggc aacctggaag aagaagtggc ctaccatgag caatacccta
     4801 ccatctacca cctgcggaag cacctcgccg attctagtga gaaggccgat ctgagactgg
     4861 tttacctggc cctggcccat atcatcaaat acagaggcca cttcctgatc gaaggcgacc
     4921 tgaacaccga gaacactgac gtggaagagc tgttcaaaca gttcgtgcag gtgtacaacc
     4981 agaccttcga ggaacaacac ctgtcggatg agaccatcga cgtggaggaa atcctgacag
     5041 aaaaagtgtc caagagcaga cgggccgaga acgtgctgaa gctatttcca aatgagaaga
     5101 aaaacggcct gtttggccag ctgatcaagc tgatcgttgg cctgcagggc aacttcaaga
     5161 aagtgttcga cctcagcgag gacgccaagc tccaattttc taaggatacc tatgaggagg
     5221 acctggaaaa ccttctggcc atgatcgggg acgagtacgc cgacctgttc ctggccgcca
     5281 aaaacctgta cgacgctatc ctgctttctg gaatcctgac cacaaccgat aataacacca
     5341 aggccaagct gagcgccagc atgatcaagc ggtacgagga gcaccagaag gacctggcac
     5401 aactgaagca gttcatcaaa gagcacctgc cagacaagta caatgaaatc ttcagcgact
     5461 ccagcaaaaa cggctatgcc ggctacatcg agggcaaaac cagccaggag gatttctaca
     5521 agtacctgaa gaagatcctg tctaagattg atggcgctga atacttcctg gagaaaattg
     5581 aacaggaaaa ttttctgagg aagcaaagaa cctttgacaa cggcgtgatc ccgcaccaga
     5641 tccacctgga ggagctgaga gccatcctga ggcgtcaggg caagtactac ccattcctca
     5701 aagagaacca ggagaagata gagcagatcc tgacatttag aatcccctac tacgtgggcc
     5761 cactggctag aggaaatagc cggttcgcct ggctgacacg gaagtctgac gagcccatca
     5821 caccttggaa cttcgacgaa gtcgtggaca aggagaagtc cgccgaggcc ttcatcgaga
     5881 gaatgaccaa ctacgacacc tacctgccta acgaaaaggt actgcccaaa cacagcctgc
     5941 tgtacgagaa gttcaccgtg ttcaacgaat tgacaaaggt tcggtacgtg acagaccggg
     6001 gcaagaccca gaacttctcc gccgagcaga agcaggagat cttcgacgac ctgttcaaga
     6061 agaaccggaa ggtcacaaag aaaaagctgg agaactatct gaagaaagaa tacgagtact
     6121 tcgacagccc tgatatcacc ggaattgagg acgagttcaa cgccagcctg ggcacctatc
     6181 atgatctgct gaagatcctg aagtctaagg acttcctgga cgatgaggaa aacgaggaaa
     6241 tcctggagga tatcgtgaag atcctgaccg tgttcgagga cagaaagatg atcagaaagc
     6301 ggctggagaa atacagcgac atcctcaccg aggaacagtt gaagaagctg gagcggcggc
     6361 actacacagg ctggggcaga ttgagcagaa agcttatcaa cggcatccgc gacaaacaga
     6421 gcggcaagac aattttagat tacctgatcg acgacgacag cagtaaccgg aacttcatgc
     6481 agctgatcaa cgatgacaat ttgagcttta aggaggaaat cgagaaggcc caggtgatcg
     6541 gagagaccga gaacctgcac gagatagtgc aagacctgcc tggcagccct gctatcaaga
     6601 agggcatcct gcagtctctg aagattgtgg acgagctcgt gaaggtgatg gggcacgaac
     6661 ctaagaacat cgtggtcgaa atggccagag agaaccagac gaccagcagg ggcagacgga
     6721 acagcaagca gcggctgaag agactggagg aggctcttaa gaacctgggc agcaacctgc
     6781 tgaaagaaca ccccgtggac aatcagcagc tgcagaacga cagactgtac ctatactacc
     6841 tgcaaaatgg caaggatatg tacacaggac aggagctgga catcgataat ctgtccaact
     6901 acgacatcga tcacatcatc ccccagagtt tcatcaagga taatagcatc gataaccgag
     6961 tgctggtgag cagcgaggaa aatagaggca agtctgataa tgtgccttct aaggaagttg
     7021 tgagaaagat gaaatctttt tggcagaaac tgctgaatgc tggcctgatc agccagagaa
     7081 aattcgacaa cctgacaaag gctgagagag gcggcctgac cgaagacgac aaagctggat
     7141 tcatcaagag acagctggtg gaaacaagac agatcaccaa gcacgtggcc aacatcctag
     7201 acagccggtt caacacagag aaggacgagg aggacaacac catcagaaac gtgaagatta
     7261 tcaccctgaa gtccaacctg gtgtcccagt tcagaaagga tttcggcctg tacaaggttc
     7321 gggaaattaa cgactaccac cacgcccacg acgcctacct gaatgccgtg gtgggcaccg
     7381 ccctgctgaa gaagtatcct caactggagc ctgagttcgt gtacggcgac taccggaaga
     7441 acaacgccag agaggaaaac aaggccacag ccaagaagca cttctacagc aatatcatga
     7501 acttcttcgc ctctgaagtg aagattgccg acgaaaccgg cgagatcgtg tgggacaaag
     7561 aaaaggacat agctaccgtg cggaaggtgc tgtcctacca ccaggtgaac atcgtgaaga
     7621 aagtggaggt gcagaaggga ggattctcca aggaaaccat cctccccaag ggcaacagca
     7681 acaagctcat ccccagaaag aacaactggg accctaagaa gtacggcggc ttcgacagcc
     7741 ccaccgtggc ttatagcgtg ctggtgacct acgatatcga gaagggaaag aaaaagaagc
     7801 tgaagaccgt gaaagagctg gtcggcatca ccatcatgga gcggagcgcc tttgaaaaga
     7861 accccatcgc ctacctggaa aaaaaaggct accagaatcc tcaggaggac gtgctgatca
     7921 aactgcctaa gtactccctg ttcgagctgg aaaacggcag acgtcggatg ctggccagcg
     7981 ctaaggaact ccaaaagggc aatcagatgg tgcttcctgc ccatctggtg accctgctgt
     8041 accacgccaa acggatcgat aagagcaaca attctgagag cctggaatac gtggaagaac
     8101 acagaaatga attcgacgag ctgctggact atatcattga tttcagcgag aagtatatcc
     8161 tggctgataa gaacctggaa aagatcaaga agctttacga gcagaacaac gaggccgaca
     8221 tcaaggagct ggccaagagc tttatcaacc tgctgacctt caccgccatg ggcgcccctg
     8281 ccgctttcaa gttcttcggc gaaacaatcg acagaaaaag atacacaagc atcaaggaac
     8341 tgctcaacgc caccctcatc caccagagca tcacaggtct gtacgagaca agaatcgacc
     8401 tgggcaagct gggagaggat cactctggcg gctcaaaaag aaccgccgac ggcagcgaat
     8461 tcgagcccaa gaagaagagg aaagtcggag gcggtggaag cggcgcaaca aacttctctc
     8521 tgctgaaaca agccggagat gtcgaagaga atcctggacc gatggtgagc aagggcgagg
     8581 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     8641 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     8701 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     8761 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     8821 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     8881 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     8941 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     9001 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     9061 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     9121 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     9181 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     9241 ccgccgggat cactctcggc atggacgagc tgtacaagtg acatcatcat caccacgcct
     9301 ctggaacagg cggacctaaa aagaagagga aggtttaagg taccgagctc ggatccacta
     9361 gtccagtgtg gtggaattct gcagatatcc agcacagtgg cggccgctcg agtctagagg
     9421 gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     9481 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     9541 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     9601 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     9661 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     9721 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     9781 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     9841 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     9901 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     9961 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
    10021 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
    10081 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
    10141 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
    10201 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
    10261 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
    10321 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    10381 ccccatggc
//
