LOCUS       sequence-362672-       10434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon usage 
            anCas(PCA) with an N-terminal NLS, C-terminal bi-partite NLS, and 
            P2A-eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10434)
  AUTHORS   Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille 
            LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S,
            Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias 
            A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu 
            L, Perez-Jimenez R
  TITLE     Evolution of CRISPR-associated endonucleases as inferred from 
            resurrected proteins.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y.
            Epub 2023 Jan 2.
  PUBMED    36593295
REFERENCE   2  (bases 1 to 10434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01265-y"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "77-90"
COMMENT     Sequence Label: pCMV-T7-NLS(SV40)-anCas(PCA)-BPNLS(SV40)-P2A-E...
FEATURES             Location/Qualifiers
     source          1..10434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(157..176)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        348..727
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        728..931
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     882..902
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     976..995
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        976..994
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1045..1065
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5221..5241
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5260..5316
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5317..5319,5320..5322,5323..6036)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5317..5319,5320..5322,5323..6033)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5623..5642)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5970..5991
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6070..6090
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6138..6155)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6144..6368
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6414..6842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6501..6520)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6711..6732
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6851..6871)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6856..7185
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7036..7171
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7098..7117
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7252..8046
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7306..7325)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7916..7935
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8220..8341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8257..8276)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8311..8330
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8390..8406)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8390..8406)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8403..8425)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8414..8430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8438..8444,8445..8462,8463..8468))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8483..8504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8621..8638)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8792..9380)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8872..8891)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9551..10342,10343..10411))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10174..10193
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10412..10434,1..82))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
       61 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
      121 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
      181 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
      241 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
      301 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
      361 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      421 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      481 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      541 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      601 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      661 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      721 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      781 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      841 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      901 tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac ccactgctta
      961 ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct agcgtttaaa
     1021 cttaagcttg gtaccgccac catgcccaag aaaaagcgga aggtgcccaa gatgaagaaa
     1081 ccatacagca tcggcctgga catcggcacc aacagcgtgg gctgggccgt gatcacagat
     1141 gattacaagg tgcccgctaa gaagatgaag gtgctgggca acaccgacag acaaagtatt
     1201 aagaaaaacc tgatcggagc cctgctgttt gacagcggag agacagctga ggccaccaga
     1261 ctgaaacgga cagctcgtag aaggtatacc agaagaaaga acagaatctg ttacctgcag
     1321 gagatcttta gcgaagagat ggccaaggtg gacgacagct tctttcaccg cctggaagaa
     1381 tctttcctgg tgcccgagga taagaagtac gatagacacc ctatcttcgg caatctggcg
     1441 gatgaagtgg cctaccacga aaactacccc accatctacc acctgagaaa gaagctggcc
     1501 gactctacag agaaagccga cctgagactg atctacctgg cccttgccca tatcatcaag
     1561 ttccggggcc acttcctgat cgagggcgac cttaacgccg aaaacaccga cgtgcagaaa
     1621 ctgttccacc agctggtgga tacctacaac cagctgttcg aggaggacca gctggatact
     1681 gagaccatcg acgcaaaggc catcctgacc gctaagatta gcaagagcag acgcctggaa
     1741 aacctgatca gccaaatccc tggacagaaa aaaaacggcc tgtttggcaa cctgatcgcc
     1801 ctgagcctgg gcctgacccc taatttcaag tctaacttcg acctgtccga ggatgccaaa
     1861 ctgcagctgt ccaaggacac ctacgaggag gatctggaca atctgcttgc tcagatcggc
     1921 gatcagtacg ccgacctatt tctcgccgcc aagaacctga gcgatgccat tctgctctcc
     1981 gacatcttga cagtcaatga cgagagcaca aaagcccctc tgtctgcttc catgatcaag
     2041 cggtacgaag aacaccagca ggacctggcc ctgctgaaac agctggtgaa ggaacagctg
     2101 cccgagaaat acaaagagat cttctctgac aaatctaaga acggatacgc cggatatatc
     2161 gatggcaaga cctcccagga ggagttctac aagtacatca aacctatcct gtccaagctc
     2221 gatggcgccg aggaattcct tgccaagatc gatagagaag attttctgcg caagcagaga
     2281 acctttgaca acggctccat cccccaccag atccacctcg aagagctgca tgcgatcctg
     2341 agaagacagg aggaatacta cccgttcctg aaggacaatc aagagaagat cgaaaaaata
     2401 ttgaccttcc gaatcccata ctacgtggga cctctggcca gaggaaatag cagattcgcc
     2461 tggctgacca gaaaatctga tgaggccatc acaccctgga actttgagga ggtcgtggat
     2521 aaagaagcct ccgcccaggc gttcatcgag cgtatgacaa acttcgatac gtacctgccc
     2581 aacgaaaagg tgcttccaaa acattctctg ctgtacgaga ccttcacagt gtacaacgag
     2641 ttgacaaaag tgaagtacgt gaccgagggc atgaccaagc ctcaatttct cagcgcggag
     2701 cagaagcaag ccatcgtgga cctgctgttc aagaagaacc ggaaggtgac agtgaaacag
     2761 cttaaggaag actatttcaa gaagatcgag tgcttcgact ccgtggacat cacaggagtt
     2821 gaagacaggt tcaacgccag cctgggcaca taccacgatc tgctgaagat catcaaggac
     2881 aaggacttcc tggacaacga ggagaatgaa gatatcctgg aagatatcgt actgacactg
     2941 acactgttcg aggatagaga gatgatcgaa aagagactgg ccaagtacgc ggacctgttc
     3001 gacaagaagg tgctgaagaa actgaagaga cggcactaca ccggatgggg cagactgtcc
     3061 cggaaactga tcaatggaat cagagataag cagagcggca agaccatcct ggattttctg
     3121 aaggccgatg gattcgccaa tagaaacttt atgcagctga ttaacgacga ttccctgagc
     3181 tttaaggagg aaatcgagaa ggcccaagtg atcggccaga ccgactccct gcacgaggtg
     3241 gtggccgact tagctggcag ccccgccatc aagaagggca tcctgcagac aatcaagatc
     3301 gtggacgagc tggtgaaggt tatgggccgg cacaaccccg aaaatatcgt catcgaaatg
     3361 gccagagaga accagaccac cgcccaaggc atcaagaata gtagacagcg gatgaagcgg
     3421 ctggaggagg tgctgaagaa actgggcagc aacatcctga aggagcaccc cgtggacaac
     3481 acccagctgc agaacgacag gctgtacctg tactacctgc aaaatggcaa ggacatgtac
     3541 accggccagg agctggacat tgacaacctc agccagtacg acattgacca catcatccct
     3601 cagagtttca tcaaggacaa cagcatcgac aacaaggtcc tcaccagcag cgaggaaaac
     3661 agaggcaaga gcgacaatgt gcctagcatc gaggtggtgc ggaagatgaa gagctactgg
     3721 cagaaactgc tgaacgccgg cctgatttct cagcgaaagt tcgataatct gaccaaggca
     3781 gagcggggtg gactgactga gagcgacaaa gctggcttca tcaagcggca gctggtcgag
     3841 acaagacaga tcaccaagca cgtggcccaa atcctggaca gcagattcaa cactgagcgg
     3901 gacgaaaacg acaaacctat cagaaacgtg aagatcatca ccctgaagtc taagctggtg
     3961 tcggacttca gaaaagactt cggcctgtac aaggttagag agattaacga ttaccaccac
     4021 gcccacgacg cctacctgaa cgccgtggtc ggaacagccc tgctgaagaa gtaccctaag
     4081 ctggagcctg agttcgtgta tggcgactac aaaaaatatg atctgagaaa gctgatcgcc
     4141 aagagcgaca aggagagagg caaggccaca gcaaagatgt tcttctacag caacatcatg
     4201 aacttcttca agacagaagt gaagctggct aatggcacaa tcatcaagag acctgtgatc
     4261 gagaccaacg aggaaaccgg cgagatcgtg tgggacaagg aaaaggattt cgccaccgtg
     4321 cggaaggtgc tgagctaccc ccaggtgaac atcgtcaaga agaccgaggt gcagaccggc
     4381 ggcttcagca aggaaagcat cctccctaag ggcaacagcg acaagctaat cccaagaaag
     4441 aacaactggg accctaagaa gtacggcgga ttcgactctc ctaccgtggc ctatagcgtg
     4501 ctggttgtgg ccaaggtgga aaagggcaag gccaagaagc tgaagaccgt gaaagaactg
     4561 gtgggcatca ccatcatgga aagaagcgcc ttcgaaaaga atcctattgc ctttctcgaa
     4621 gctaagggct accaggacat ccaggaggac ctgataatca aactgcctaa atacagcctg
     4681 ttcgagctgg agaacggccg gagacggctc ctggctagcg ctaaagagct acagaagggc
     4741 aacgagatgg tgctgcctgc ccacctggtg accttcctgt accatgccag ccggatcgat
     4801 aaaagcacgg gctctagcga aaacttagag caaaagcagg agtacgtgga acagcacaag
     4861 cacgagttcg atgagatcct ggactacatc attgactttt ccgagagata catcctggcc
     4921 gataagaacc tggagaagat caaaagcctg tataaccaga acgacgactc cgacatcaac
     4981 gagttggcct cttctttcat caatctgttc accttcaccg ccctgggcgc ccctgctgcc
     5041 tttaagttct tcgacgctac aatcgataga aagcggtata ccagcaccaa ggaggtgctg
     5101 aatgctacac tgatccacca gagcatcacc ggactgtacg agactagaat cgacctttcc
     5161 cagttaggcg gcgatcactc tggcggctca aaaagaaccg ccgacggcag cgaattcgag
     5221 cccaagaaga agaggaaagt cggaggcggt ggaagcggcg caacaaactt ctctctgctg
     5281 aaacaagccg gagatgtcga agagaatcct ggaccgatgg tgagcaaggg cgaggagctg
     5341 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5401 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     5461 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     5521 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5581 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5641 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5701 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     5761 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     5821 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     5881 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     5941 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     6001 gggatcactc tcggcatgga cgagctgtac aagtgacatc atcatcacca cgcctctgga
     6061 acaggcggac ctaaaaagaa gaggaaggtt taagcggccg ctcgagtcta gagggcccgt
     6121 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     6181 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     6241 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     6301 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     6361 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     6421 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6481 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6541 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6601 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6661 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6721 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6781 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6841 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     6901 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     6961 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     7021 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     7081 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
     7141 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
     7201 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
     7261 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     7321 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     7381 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
     7441 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     7501 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     7561 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     7621 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     7681 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     7741 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
     7801 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     7861 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     7921 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     7981 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     8041 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     8101 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     8161 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     8221 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     8281 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     8341 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
     8401 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     8461 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     8521 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     8581 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     8641 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     8701 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     8761 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     8821 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     8881 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     8941 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     9001 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     9061 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     9121 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     9181 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     9241 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     9301 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     9361 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     9421 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     9481 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     9541 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     9601 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     9661 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     9721 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     9781 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     9841 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     9901 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     9961 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    10021 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    10081 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    10141 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    10201 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    10261 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    10321 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    10381 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tatt
//
