LOCUS       sequence-362677-       10383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV and T7 promoter expression plasmid for human codon usage 
            anCas(FCA) with an N-terminal NLS, C-terminal bi-partite NLS, and 
            P2A-eGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Alonso-Lerma B, Jabalera Y, Samperio S, Morin M, Fernandez A, Hille 
            LT, Silverstein RA, Quesada-Ganuza A, Reifs A, Fernandez-Penalver S,
            Benitez Y, Soletto L, Gavira JA, Diaz A, Vranken W, Sanchez-Mejias 
            A, Guell M, Mojica FJM, Kleinstiver BP, Moreno-Pelayo MA, Montoliu 
            L, Perez-Jimenez R
  TITLE     Evolution of CRISPR-associated endonucleases as inferred from 
            resurrected proteins.
  JOURNAL   Nat Microbiol. 2023 Jan;8(1):77-90. doi: 10.1038/s41564-022-01265-y.
            Epub 2023 Jan 2.
  PUBMED    36593295
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-022-01265-y"; journalName: "Nat Microbiol"; date: 
            "2023-01"; volume: "8"; issue: "1"; pages: "77-90"
COMMENT     Sequence Label: pCMV-T7-NLS(SV40)-anCas(FCA)-BPNLS(SV40)-P2A-E...
FEATURES             Location/Qualifiers
     source          1..10383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(175..194)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        366..745
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        746..949
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     900..920
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     994..1013
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        994..1012
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1035..1055
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5127..5147
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5166..5222
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(5223..5225,5226..5228,5229..5942)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5223..5225,5226..5228,5229..5939)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5529..5548)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5876..5897
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5976..5996
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(6105..6122)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6111..6335
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6381..6809
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6468..6487)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6678..6699
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6818..6838)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6823..7152
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7003..7138
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7065..7084
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7219..8013
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7273..7292)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7883..7902
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8187..8308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8224..8243)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8278..8297
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8357..8373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8357..8373)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8370..8392)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8381..8397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8405..8411,8412..8429,8430..8435))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8450..8471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8588..8605)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8759..9347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8839..8858)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9518..10309,10310..10378))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10141..10160
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(10379..10383,1..100))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
       61 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
      121 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
      181 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
      241 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
      301 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
      361 gcgttgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      421 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      481 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      541 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      601 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      661 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      721 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
      781 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      841 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
      901 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
      961 tagagaaccc actgcttact ggcttatcga aattaatacg actcactata gggagaccca
     1021 agctggctag catgcccaag aaaaagcgga aggtgcccaa gatgaagaag gactacagca
     1081 tcggcctgga catcggcacc aattctgttg gctgggccgt gaccgacgac aactacaacc
     1141 tcgtgcggaa gaaaatgaag gtgctgggca acaccgacaa gaagtccatc ggaaaggccc
     1201 tgtggggcgt cagactgttt gatgccgctg agacagccga ggaacggcgg atgcacagaa
     1261 ccaccagaag gcggtacacc cggcggagac agagaatcga tctgctgcaa gagatcttcc
     1321 aagaggaaat cagcaaggtc gaccccagct tctttatccg gctgaacgag agcagactgc
     1381 accccgagga caagaccgac gatagacacc ctctgttcgg cgacaacgag acagacaagg
     1441 actaccacaa gcagtacccc accatctacc acctgagaaa gcacctgatg gaaagcgacg
     1501 agaagcacga catccggctg gtgtatctgg ccctgcacca catcatcaag taccggggcc
     1561 acttcctgat cgagggcgac ctgaatagcg agaacaccga cgtggaagaa ctgttcaagc
     1621 agctggtcca ggtgttcaac gacaccttcg aggaagaaca cctgagcgag gaagccatcg
     1681 acatcgagga aatcctgacg gataagaagt cccggtccac cagagccaaa gaggtggtca
     1741 agctgttcgg cagcgagaag aagcaggcct gtatcagcgc cctgatcaag ctgatcgtgg
     1801 gcctgaaggg caacctgaag aaggtgttcg gggacgccga ggacaccagc atccacttca
     1861 gcaaggacaa ctacgaagag gacctggaag ccattcggga catcatcccc gacgagtacg
     1921 ccgatctgtt cgaggccatc aaagccctgt acgacgccat cgtgctgtct ggcattctcg
     1981 gcggcagcac ctctaacacc aaggccaaag tgtccgccag catgatcgcc agatacgagc
     2041 agcaccagaa agacctgaag cagctgaaac agttcgtgaa agagcatctg cccgagaagt
     2101 acaacgagat cttcagcgac aacaccaaga acggctacag cgcctacatc gaaggcggca
     2161 ccagccaaga ggacttctac aagtatctga agaagatcct gaaagagctg gaagaggccg
     2221 agtacctgct ggaagaaatc gagaacgaga acttcctgcc taagcagcgg acctccgaca
     2281 acggcgtgat cccttatcag atccacctcg aggaactgcg ggccatcctg aagaaccagg
     2341 gcaagtacta cccattcctc aaagagaacg ccgagaagat cgagtctatc ctgaccttca
     2401 gaatccccta ctacgtggga ccactggcca gaggcaatgg cagattcgcc tggatgatca
     2461 gaaagaagga cggcaagatc accccttgga acttcgacga ggtggtggac aaagagaagt
     2521 ccgccgagga attcatcgag cggatgacca acaactgcac ctacctgcct ggcgagaacg
     2581 tgctgccaaa gaacagcctg ctgtacgaga agttcaccgt gctgaatgag ctgaacaacg
     2641 tgcggctgac caccgataag ggcaagacca gaagattcag cgccgagcag aagcaagaga
     2701 ttttcgacga cctgttccgg aagaaccgga aagtgaccaa gaagaagctc gaggactacc
     2761 tgaagcgcga gtacgaagag ttcgactccg ccgacatctc cggcatcgat ggcgagttta
     2821 agagcagcct gggcagctac cacgacttct gcaagatcgt ggtcaagggc aacagcctgg
     2881 acgaggaaga ttacaaggat atcatcgaag agatcattaa gtggctgacc gtgttcgagg
     2941 accggaagat gctgcggaga aggctggaaa agtactccga gatcctgacc gaggaacaga
     3001 tcaagaagat ctgcaagcgg cactacaccg gctggggcag actgtctaga aagctgctga
     3061 ccggcatccg ggacaaagaa accggcaaga gcatcatcga cgtgctgcgg gaaaccgaca
     3121 gcagcaaccg gaacttcatg cagctgctga gcgacgagga cctgagcttc aaagaagaga
     3181 tcgagcaggc caacgccgag gccgaaggcg aaaatctgca cgagatcgtc gaggatctgc
     3241 ctggctctcc cgccatcaag agaggaattc tgcaggccct gaagattgtg gatgagatcg
     3301 tgaaagtgat ggggcacgag cccaagaaca tcttcgtgga aatggcccgc gagaaccaga
     3361 aaaccggcag aggcagacgc tccaccaaga aaagactgaa gcgcctgcaa gaggccctca
     3421 agaacctggg ctccaacctg ctcaaagagc tgccccggga cgataacgag ctgcggaacg
     3481 atagactgtt cctgtactac acccagatgg gcaagtgcat gtacaccggc gagcccatcg
     3541 atctggacga cctgagcaac tacgacatcg atcacatcat tccccagagc ttcatcaagg
     3601 acgactccct ggacaaccgg gtgctcgtgt ccagagagga aaacgcccgc aagaccgata
     3661 acttcccatc tcctgagatc cggcggaaga tgaagtcctt ctggcaaatg ctgctgaagg
     3721 ccggactgat ctctaagaag aagtttgaca gactgaccag agccgacaga ggcgacttca
     3781 ccgatgatga gctggccggc tttattgccc ggcagctggt ggaaacccgg cagatcacaa
     3841 aacacgtggc cacactgctg aagcagagat accccaccga gaaggatgag gaagataaga
     3901 ccatccggaa cgccaagatt gtgtccgtga aggccaatct ggtgtccgag ttcagacagg
     3961 acttcggcct gtacaagtgc cgggaagtga acgactacca tcacgcccac gacgcctacc
     4021 tgaatgccgt tgtgggaaac gccctgctga agaagtaccc tcagctggcc gccgagttta
     4081 tcaagggcga ctacagaaag aacaacgccc gggaagagaa caaggccaat gccaagatgc
     4141 acttttacag caacatcatg aacagcttca cctccgacgt gaagatcgcc gacgagacag
     4201 gcgagatcgt gtgggacaaa gagaaagata tcgccaccgt gcggaaagtc atgaactacc
     4261 accaggtgct gatcacccgc aaggtggaag aggaaaaagg cggcttcttc gaccagacca
     4321 tcctgagcaa gggcaatagc aagaagctga tcccgctgaa gaaaaatctg gaccctgaga
     4381 agtacggcgg ctacaactct cctaccgtgg cctactctgt gctggtcgag tacgatatcg
     4441 agaagggcaa aaagaaaaaa ctcaagaccg ttaagcagct cgtgggcatc cccatcagag
     4501 agagagccaa gctggaaaaa aaccccatca agtatctcga gaaaaagggc tatcagaacc
     4561 ctaaggtgga cctgctgatc aagatcccca agaactccct gttcgagctg gacggcggca
     4621 ggcgaagaat actggccgct gcaaaagagc tgaaaaacgc caatcagctg gtgctccccg
     4681 ccgaagagta taccctgctg gataaggtgg ccaaaatcat taagaagaac aacagcgaga
     4741 gcatcgagta cgtcgaggaa cacctgtctg agttcgatga gctgctggaa agcctgatcg
     4801 actacagccc taagctggct ctgcaggaca agaatctgga aaagatcaaa gaggccttcg
     4861 aacagctgaa cctggccgat aagaaagaag tggccaaaga gattatcaac ctgctgcact
     4921 gcaccgccac caccgctaat gccgctctga agtttctcgg aggctccaag aacagaatgc
     4981 gctacacctc catcaaagaa ctgctgaacg ccagcctgat ccaccagtct atcaccggcc
     5041 tgtatgagac acggatcgac ctgggaaagc tgggcgaaga tcactctggc ggctcaaaaa
     5101 gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtcgga ggcggtggaa
     5161 gcggcgcaac aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac
     5221 cgatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5281 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5341 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5401 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5461 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5521 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5581 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     5641 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     5701 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     5761 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     5821 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     5881 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     5941 gacatcatca tcaccacgcc tctggaacag gcggacctaa aaagaagagg aaggtttaag
     6001 gtaccgagct cggatccact agtccagtgt ggtggaattc tgcagatatc cagcacagtg
     6061 gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc
     6121 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     6181 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     6241 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     6301 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     6361 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     6421 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     6481 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     6541 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     6601 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     6661 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     6721 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     6781 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     6841 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
     6901 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6961 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     7021 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     7081 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     7141 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca
     7201 ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct
     7261 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     7321 gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     7381 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     7441 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     7501 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     7561 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     7621 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     7681 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     7741 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     7801 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     7861 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     7921 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     7981 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     8041 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     8101 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     8161 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     8221 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8281 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     8341 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     8401 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     8461 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     8521 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     8581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     8641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     8701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     8761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     8821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     8881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     8941 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     9001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     9061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     9121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     9181 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     9241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     9301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     9361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     9421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     9481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     9541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     9601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     9661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     9721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     9781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     9841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     9901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     9961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    10021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    10081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    10141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    10201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    10261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    10321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    10381 tct
//
