LOCUS       sequence-362680-       12790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12790)
  AUTHORS   Henry GL, Davis FP, Picard S, Eddy SR
  TITLE     Cell type-specific genomics of Drosophila neurons.
  JOURNAL   Nucleic Acids Res. 2012 Oct;40(19):9691-704. doi: 
            10.1093/nar/gks671. Epub 2012 Aug 1.
  PUBMED    22855560
REFERENCE   2  (bases 1 to 12790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gks671"; journalName: "Nucleic Acids Res"; date: 
            "2012-10"; volume: "40"; issue: "19"; pages: "9691-704"
COMMENT     Sequence Label: pMUH_unc84_2XGFP
FEATURES             Location/Qualifiers
     source          1..12790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        31..135
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(136..204,205..996)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(354..373)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1167..1755
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1656..1675
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    1842..1911
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     primer_bind     complement(2136..2154)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2606..2677,3079..3352,3427..4081,4143..4458,4662..4793,
                     4864..5478)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    6508..6602
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    6636..6730
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        6761..6999
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     6946..6967
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     RBS             9646..9654
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             join(10451..10453,10454..10456,10457..11167)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(11189..11191,11192..11194,11195..11905)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     intron          12073..12138
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12268..12288
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12560..12694
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12597..12616)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12651..12670
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12736..12754)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
       61 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
      121 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
      181 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      241 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      301 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      361 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      421 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
      481 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
      541 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
      601 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
      661 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
      721 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
      781 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
      841 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
      901 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
      961 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     1021 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     1081 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     1141 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     1201 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     1261 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     1321 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     1381 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     1441 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1501 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1561 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1621 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1681 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1741 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1801 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1861 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1921 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1981 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     2041 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     2101 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt
     2161 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta
     2221 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat
     2281 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc
     2341 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt
     2401 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa
     2461 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct
     2521 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca
     2581 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg
     2641 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat
     2701 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtct gcggcgtgag
     2761 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag
     2821 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag
     2881 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat
     2941 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga
     3001 tacttcgggg tactttttgt tcggggttag atgagcataa cgcttgtagt tgatatttga
     3061 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc
     3121 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt
     3181 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt
     3241 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta
     3301 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt
     3361 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa
     3421 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg
     3481 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat
     3541 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca
     3601 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc
     3661 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc
     3721 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg
     3781 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg
     3841 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct
     3901 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca
     3961 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga
     4021 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct
     4081 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc
     4141 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg
     4201 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat
     4261 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag
     4321 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc
     4381 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg
     4441 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat
     4501 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt
     4561 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact
     4621 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg
     4681 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca
     4741 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc
     4801 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt
     4861 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc
     4921 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca
     4981 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc
     5041 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta
     5101 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata
     5161 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa
     5221 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg
     5281 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg
     5341 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg
     5401 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg
     5461 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc
     5521 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     5581 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     5641 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     5701 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     5761 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     5821 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     5881 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     5941 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     6001 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     6061 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     6121 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     6181 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     6241 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     6301 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     6361 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     6421 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     6481 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
     6541 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     6601 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
     6661 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     6721 gtcctccgag cggagactct agcgctagcg acgtcgagcg ccggagtata aatagaggcg
     6781 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa
     6841 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt
     6901 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc
     6961 tgccaagaag taattattga atacaagaag agaactctga atagatctgc ggccgcggct
     7021 cgagggtacc tctagaatgg ctcccgcaac ggaagccgac aacaacttcg acacccatga
     7081 atggaaatcg gaattcgcat ccacacgctc tggacgcaat tctccaaaca tttttgcaaa
     7141 agttcgccgg aagcttctcc tgactccacc agttcgaaac gccagatcgc cacgtcttac
     7201 cgaagaagag ctggatgctt tgacaggcga cttaccatac gcaaccaact acacatacgc
     7261 atacagcaaa atctacgatc catccttgcc ggaccactgg gaagtgccaa accttggtgg
     7321 tactacttca ggatcactct ctgagcagga gcactggtca gcggccagtc tcagcagaca
     7381 gcttctctat atcctccgtt tccccgtcta ccttgttctt cacgtcatca cctacatttt
     7441 ggaagctttc taccacgtca tcaagatcac tagcttcacc atctgggact acctgttgta
     7501 tttggtgaaa ctcgcgaaaa ctcgttacta cgcctaccaa gatcatcgtc gccgtacagc
     7561 tctcattcgc aaccggcaag agccattctc cactaaggct gctcgttcta ttcgtcgatt
     7621 ctttgagatc cttgtctacg tcgtgcttac tccttacaga atgctcacaa gaagtaacaa
     7681 tggcgtggaa cagtaccagt accgttcgat caaggatcaa ttggaaaatg agagagctag
     7741 cagaatgacg acaagatccc aaacattgga aagaagccgc aagtttgatg gattatcgaa
     7801 atcaccagca cgccgagcag ctccagcctt tgtgaagact agtacaatta ccagaatcac
     7861 tgccaaggtg ctctcgagct ctccattcgg agaaggaacg tccgaaaata taaccccgac
     7921 tgttgtgact actagaacag tgaagcaacg ctcagttacc ccaagattcc gccaaacccg
     7981 tgccactcgt gaagctataa ctcgagcact cgatactccg gaactcgaaa tcgacacacc
     8041 actctccaca tatggacttc gaagccgagg actgagtcat ctgaatactc ctgaaccaac
     8101 ttttgacatt ggtcatgctg ctgcaacttc cacgcctttg ttcccacaag aaacttacaa
     8161 ttatcaatac gaagaagcga caggaaataa gattaaaact gcattcactt ggctaggtta
     8221 cttgatattg ttcccgttct ttgcggcacg acatgtatgg tatacgttct acgattatgg
     8281 aaagagtgcc tacatgaagc tgaccaatta tcagcaagcg ccaatggaga ctattcatgt
     8341 cagagatatc aacgaaccgg caccaagttc atcagatgtt catgatgctg ttggtgtttc
     8401 ttggagaatt cgaattgccg atttcttgag ctcattcgta gcaacaatcg ttgaagcgca
     8461 tcaagtggta tttgcaatgt tcaaaggagg aattgtcgag acagtttcct attttggagg
     8521 actatttgct ggtcctaccg ataagaaatc atcaaagttc tcgtggtgtc aaattctcgg
     8581 tctacttctg gctcttctct tcgccatctt tctccttgga ttcctgacat ctgacaacac
     8641 agcaataaga gttaaagaaa ttaccaaaga taagaatgca tctaagaagt cggaaggatc
     8701 cctcccagct gtgccaatct ggatttcagc tgcaaatcac gttaaacatt acacatggat
     8761 ggtgaaggaa tttgttgtag atattgcatt tgacacgtac aactatggaa agtcgacgat
     8821 tggtagactt ggcactactc cacgttatgc ttgggacctg attgcaagcg gatgtggcgc
     8881 tgttggaaat ggcttaaaat ctgtgctctc atcgagtttt cgattcatcg atttttgtgc
     8941 tggaaagcta ttttactatg gctcagatgg gttcttgtca gcaaacaagt ctatcggaac
     9001 ctttttcaac ggttgctacg agaccttgta caacggatgc acagcaattg ttggccatac
     9061 aaagagcttc atctacaatg cttcaaatgc tgtttacaac tttttctcaa ctatctttgc
     9121 cggtctctta aacttttcta cttcttccca aaactccatt ctttctcttc tcaagtcatt
     9181 tggcaccgga atcactaaca ttttttataa cttcatttat gcaccaatcg ctggagtgtt
     9241 caactttgct ggtgataact acatgtattt cttcaatgag gtagcggcag tctttggaaa
     9301 agtgtacaac tccgtggttt ccgtgctcaa aactgtaatt aactggattc tcttcctcat
     9361 tgcctaccca ttcagtttgt gcactcgtgc ttggattcgc atcagccaat atgctccaga
     9421 agatgttgtt caagtgattc caattccaca agctattacc ccaactccgg atgtggagcg
     9481 tattgttgaa gagccactga gaaaagtcac cgatgtggag gacgaagaac tagtgataat
     9541 tcccgccccc gcacctaaac ctatcccagt cccagcgcca actccggccc cagtaattat
     9601 ccatcagact aacgttgttg agactgttga caaagatgcc atcattaagg aggtaacgga
     9661 gaagcttcgc gccgagttgt ccgcccaatt ccagcaagag cttagcgcaa agtttgagca
     9721 aaactacaac acaattattg agcaactgaa aatggaaaac accaacattc aatatgataa
     9781 gaatcatttg gaagctatca tccgtcaaat gatctacgag tatgacacgg ataaaactgg
     9841 gaaagttgac tatgccctgg agagctcagg tggagctgtt gtgtcaacaa gatgctcgga
     9901 gacgtacaaa agctacacaa ggctggaaaa gttttgggat atcccaatct actatttcca
     9961 ttactctcca agagttgtca ttcagagaaa ttccaaatcc ctgtttcctg gggaatgctg
    10021 gtgcttcaaa gaatcccgtg gctacattgc tgtcgagctg tctcatttca ttgatgtttc
    10081 tagcatcagc tatgagcaca ttggatcaga agttgctcca gaagggaacc ggtcgagtgc
    10141 tccaaaggga gtcctcgttt gggcttacaa gcagattgac gacctgaact cgagagtttt
    10201 gattggcgac tacacttatg atcttgatgg cccgccactt caattcttcc ttgccaagca
    10261 caaacccgat tttcctgtca agtttgtgga gctcgaggtg acaagcaatt acggagctcc
    10321 gttcacatgt ctctaccgcc ttcgtgttca tggaaaagtt gttcaagttg gaggcgtggc
    10381 gcgcctcgcc gcagcctccg gcggcggagg ctctggcgga gggggctccc tcgcagcagc
    10441 ctctgaattc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
    10501 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg cgcggcgagg gcgagggcga
    10561 tgccaccaac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
    10621 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
    10681 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
    10741 caccatcagc ttcaaggacg acggcaccta caagacccgc gccgaggtga agttcgaggg
    10801 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
    10861 cctggggcac aagctggagt acaactttaa cagccacaac gtctatatca ccgccgacaa
    10921 gcagaagaac ggcatcaagg ccaacttcaa gatccgccac aacgtggagg acggcagcgt
    10981 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
    11041 cgacaaccac tacctgagca cccagtccgt gctgagcaaa gaccccaacg agaagcgcga
    11101 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
    11161 gtacaaggga ggcggaggtg caagatctat ggtgagcaag ggcgaggagc tgttcaccgg
    11221 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg
    11281 cggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac
    11341 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
    11401 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
    11461 aggctacgtc caggagcgca ccatcagctt caaggacgac ggcacctaca agacccgcgc
    11521 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
    11581 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactttaaca gccacaacgt
    11641 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
    11701 cgtggaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
    11761 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgtgc tgagcaaaga
    11821 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
    11881 tctcggcatg gacgagctgt acaagagatc tgaattctct gctgcggctc tgtccggcgg
    11941 cggagggtca ggaggcggcg gttccgccgc cgcactggcg cgcctctaat ctagaggatc
    12001 tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc tacagagatt
    12061 taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact actgattcta
    12121 attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca gtggtggaat
    12181 gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga tgatgaggct
    12241 actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga agaccccaag
    12301 gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa tagaactctt
    12361 gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa gaaaattatg
    12421 gaaaaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
    12481 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    12541 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    12601 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    12661 caaactcatc aatgtatctt atcatgtctg gatcgatctg gccggccgtt taaacgaatt
    12721 cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
    12781 tggtttctta
//
