LOCUS       sequence-362699-        6752 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human KIF5A(∆exon27) in insect cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Nakano J, Chiba K, Niwa S
  TITLE     An ALS-associated KIF5A mutant forms oligomers and aggregates and 
            induces neuronal toxicity.
  JOURNAL   Genes Cells. 2022 Apr 17. doi: 10.1111/gtc.12936.
  PUBMED    35430760
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Cells. 2022 Apr 17. doi: 10.1111/gtc.12936."
COMMENT     Sequence Label: pSN526
FEATURES             Location/Qualifiers
     source          1..6752
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(395..400,401..417,418..423))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(494..527)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        604..695
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     620..637
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             join(4570..4593,4594..4629,4630..4653)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     polyA_signal    4802..4936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4839..4858)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4893..4912
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5057..5222
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5412..6000
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5901..5920
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  6135..6358
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(6425..6752,1..206))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca
       61 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat
      121 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc
      181 gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac
      241 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata
      301 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga
      361 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt
      421 caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag
      481 cagcgaagtc gccataactt cgtatagcat acattatacg aagttatctg taactataac
      541 ggtcctaagg tagcgagttt aaacactagt atcgattcgc gacctactcc ggaatattaa
      601 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
      661 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
      721 ccatcgggcg cgatggcgga gaccaacaac gaatgtagca tcaaggtgct ctgccgattc
      781 cggcccctga accaggctga gattctgcgg ggagacaagt tcatccccat tttccaaggg
      841 gacgacagcg tcgttattgg ggggaagcca tatgtttttg accgtgtatt ccccccaaac
      901 acgactcaag agcaagttta tcatgcatgt gccatgcaga ttgtcaaaga tgtccttgct
      961 ggctacaatg gcaccatttt tgcttatgga cagacatcct cagggaaaac acataccatg
     1021 gagggaaagc tgcacgaccc tcagctgatg ggaatcattc ctcgaattgc ccgagacatc
     1081 ttcaaccaca tctactccat ggatgagaac cttgagttcc acatcaaggt ttcttacttt
     1141 gaaatttacc tggacaaaat tcgtgacctt ctggatgtga ccaagacaaa tctgtccgtg
     1201 cacgaggaca agaaccgggt gccatttgtc aagggttgta ctgaacgctt tgtgtccagc
     1261 ccggaggaga ttctggatgt gattgatgaa gggaaatcaa atcgtcatgt ggctgtcacc
     1321 aacatgaatg aacacagctc tcggagccac agcatcttcc tcatcaacat caagcaggag
     1381 aacatggaaa cggagcagaa gctcagtggg aagctgtatc tggtggacct ggcagggagt
     1441 gagaaggtca gcaagactgg agcagaggga gccgtgctgg acgaggcaaa gaatatcaac
     1501 aagtcactgt cagctctggg caatgtgatc tccgcactgg ctgagggcac taaaagctat
     1561 gttccatatc gtgacagcaa aatgacaagg attctccagg actctctcgg gggaaactgc
     1621 cggacgacta tgttcatctg ttgctcacca tccagttata atgatgcaga gaccaagtcc
     1681 accctgatgt ttgggcagcg ggcaaagacc attaagaaca ctgcctcagt aaatttggag
     1741 ttgactgctg agcagtggaa gaagaaatat gagaaggaga aggagaagac aaaggcccag
     1801 aaggagacga ttgcgaagct ggaggctgag ctgagccggt ggcgcaatgg agagaatgtg
     1861 cctgagacag agcgcctggc tggggaggag gcagccctgg gagccgagct ctgtgaggag
     1921 acccctgtga atgacaactc atccatcgtg gtgcgcatcg cgcccgagga gcggcagaaa
     1981 tacgaggagg agatccgccg tctctataag cagcttgacg acaaggatga tgaaatcaac
     2041 caacaaagcc aactcataga gaagctcaag cagcaaatgc tggaccagga agagctgctg
     2101 gtgtccaccc gaggagacaa cgagaaggtc cagcgggagc tgagccacct gcaatcagag
     2161 aacgatgccg ctaaggatga ggtgaaggaa gtgctgcagg ccctggagga gctggctgtg
     2221 aactatgacc agaagtccca ggaggtggag gagaagagcc agcagaacca gcttctggtg
     2281 gatgagctgt ctcagaaggt ggccaccatg ctgtccctgg agtctgagtt gcagcggcta
     2341 caggaggtca gtggacacca gcgaaaacga attgctgagg tgctgaacgg gctgatgaag
     2401 gatctgagcg agttcagtgt cattgtgggc aacggggaga ttaagctgcc agtggagatc
     2461 agtggggcca tcgaggagga gttcactgtg gcccgactct acatcagcaa aatcaaatca
     2521 gaagtcaagt ctgtggtcaa gcggtgccgg cagctggaga acctccaggt ggagtgtcac
     2581 cgcaagatgg aagtgaccgg gcgggagctc tcatcctgcc agctcctcat ctctcagcat
     2641 gaggccaaga tccgctcgct tacggaatac atgcagagcg tggagctaaa gaagcggcac
     2701 ctggaagagt cctatgactc cttgagcgat gagctggcca agctccaggc ccaggaaact
     2761 gtgcatgaag tggccctgaa ggacaaggag cctgacactc aggatgcaga tgaagtgaag
     2821 aaggctctgg agctgcagat ggagagtcac cgggaggccc atcaccggca gctggcccgg
     2881 ctccgggacg agatcaacga gaagcagaag accattgatg agctcaaaga cctaaatcag
     2941 aagctccagt tagagctaga gaagcttcag gctgactacg agaagctgaa gagcgaagaa
     3001 cacgagaaga gcaccaagct gcaggagctg acatttctgt acgagcgaca tgagcagtcc
     3061 aagcaggacc tcaagggtct ggaggagaca gttgcccggg aactccagac cctccacaac
     3121 cttcgcaagc tgttcgttca agacgtcacg actcgagtca agaaaagtgc agaaatggag
     3181 cccgaagaca gtggggggat tcactcccaa aagcagaaga tttcctttct tgagaacaac
     3241 ctggaacagc ttacaaaggt tcacaaacag ctggtacgtg acaatgcaga tctgcgttgt
     3301 gagcttccta aattggaaaa acgacttagg gctacggctg agagagttaa ggccctggag
     3361 ggtgcactga aggaggccaa ggagggcgcc atgaaggaca agcgccggta ccagcaggag
     3421 gtggaccgca tcaaggaggc cgttcgctac aagagctcgg gcaaacgggg ccattctgcc
     3481 cagattgcca aacccgtccg gcctggccac tacccagcat cctcacccac caacccctat
     3541 ggcacccgga gccctgagtg catcagttac accaacagcc tcttccagaa ctaccagaat
     3601 ctctacctgc aggccacacc cagctccacc tcagatatgt actttgcaaa ctcctgtacc
     3661 agcagtggag ccacatcttc tggcggcccc ttggcttcct accagaaggc caacatggac
     3721 aatggagtga cctgccgtgt ggctatgagg ctgaggacca ggccaagctt ttccctctcc
     3781 accaagagac agcagccagc taatctccca cacccacggc tgcatacctg cactttcagt
     3841 ttcgggcttt cgaatatggt cagcaaaggt gaagcggtaa taaaggagtt catgcgcttc
     3901 aaggtgcaca tggaaggtag catgaatgga catgagtttg agattgaggg agaaggtgag
     3961 ggcagaccat acgaaggaac acagacggct aagctcaaag tgaccaaggg aggcccgttg
     4021 cctttctctt gggacattct tagtccccaa ttcatgtatg gaagtagagc cttcacgaaa
     4081 catccggcgg atatacccga ttattacaag cagtcctttc ccgagggatt caaatgggag
     4141 cgtgtaatga attttgagga tggaggcgca gtaacagtta cccaagacac ttcactggag
     4201 gatggtacac ttatttacaa agtcaaactt cgcggcacta attttccgcc tgacggtccc
     4261 gttatgcaga aaaaaacaat gggatgggaa gcctcgactg aacgtctgta ccctgaggac
     4321 ggcgtcctga aaggtgacat taaaatggct ctgcgcctca aagacggtgg acgttacctt
     4381 gctgacttta aaactactta caaagcaaaa aaaccggtac aaatgcccgg cgcgtacaac
     4441 gtggatcgca agttggacat tacttctcat aacgaggatt acaccgtcgt tgagcagtat
     4501 gagaggagcg aaggccgcca tagcactgga ggaatggatg aattgtataa actcgagggc
     4561 agtggcagtt ggtcgcatcc tcaattcgag aagggcggcg gcagtggcgg cggcagtggc
     4621 ggcagtgcct ggagtcatcc acaatttgag aagtaaagcc tgcagtctcg acaagcttgt
     4681 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4741 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     4801 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     4861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     4921 ttatcatgtc tggatctgat cactgcttga gcctagaaga tccggctgct aacaaagccc
     4981 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actatcataa cccctagggt
     5041 atacccatct aattggaacc agataagtga aatctagttc caaactattt tgtcattttt
     5101 aattttcgta ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct
     5161 aaataatcct taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca
     5221 caaccggttg acttgggtca actgtcagac caagtttact catatatact ttagattgat
     5281 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5341 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5401 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5461 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5521 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5581 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5641 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5701 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5761 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5821 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5881 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5941 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6001 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6061 ttctttcctg cgttatcccc tgattgactt gggtcgctct tcctgtggat gcgcagatgc
     6121 cctgcgtaag cgggtgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac
     6181 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg
     6241 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt
     6301 gcaaattgcc cgtcgtatta aagaggggcg tggccaaggg catgtaaaga ctatattcgc
     6361 ggcgttgtga caatttaccg aacaactccg cggccgggaa gccgatctcg gcttgaacga
     6421 attgttaggt ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca
     6481 actttgtata gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat
     6541 aagcaccaag cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct
     6601 gcctccggtg ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct
     6661 caaacttggg cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag
     6721 caagcgcgat gaatgtctta ctacggagca ag
//
