LOCUS       sequence-362706-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression vector containing N-terminal leaderless 
            DsbC-6His-TEV fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Venkatesan M, Semper C, Skrivergaard S, Di Leo R, Mesa N, Rasmussen 
            MK, Young JF, Therkildsen M, Stogios PJ, Savchenko A
  TITLE     Recombinant production of growth factors for application in cell 
            culture.
  JOURNAL   iScience. 2022 Sep 3;25(10):105054. doi: 10.1016/j.isci.2022.105054.
            eCollection 2022 Oct 21.
  PUBMED    36157583
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-09-3"; volume: "25(10):105054. doi"; pages: " 
            10.1016/j.isci.2022.105054. eCollection 2022 Oct 21"
COMMENT     Sequence Label: pMCSG53-DsbC
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    19..43
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             58..80
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             766..783
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
       61 gtttaacttt aagaaggaga tatacatatg atgaaagatg acgcggcaat tcaacaaacg
      121 ttagccaaaa tgggcatcaa aagcagcgat attcagcccg cgcctgtagc tggcatgaag
      181 acagttctga ctaacagcgg cgtgttgtac atcaccgatg atggtaaaca tatcattcag
      241 gggccaatgt atgacgttag tggcacggct ccggtcaatg tcaccaataa gatgctgtta
      301 aagcagttga atgcgcttga aaaagagatg atcgtttata aagcgccgca ggaaaaacac
      361 gtcatcaccg tgtttactga tattacctgt ggttactgcc acaaactgca tgagcaaatg
      421 gcagactaca acgcgctggg gatcaccgtg cgttatcttg ctttcccgcg ccaggggctg
      481 gacagcgatg cagagaaaga aatgaaagct atctggtgtg cgaaagataa aaacaaagcg
      541 tttgatgatg tgatggcagg taaaagcgtc gcaccagcca gttgcgacgt ggatattgcc
      601 gaccattacg cacttggcgt ccagcttggc gttagcggta ctccggcagt tgtgctgagc
      661 aatggcacac ttgttccggg ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa
      721 caccaaaaaa tgaccagcgg taaaggatca actagtggtt ctggtcacca tcatcatcat
      781 cattcttctg gtgta
//
