LOCUS       sequence-362712-         231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. coli expression vector containing N-terminal 6His-GB1-TEV 
            fusion..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 231)
  AUTHORS   Venkatesan M, Semper C, Skrivergaard S, Di Leo R, Mesa N, Rasmussen 
            MK, Young JF, Therkildsen M, Stogios PJ, Savchenko A
  TITLE     Recombinant production of growth factors for application in cell 
            culture.
  JOURNAL   iScience. 2022 Sep 3;25(10):105054. doi: 10.1016/j.isci.2022.105054.
            eCollection 2022 Oct 21.
  PUBMED    36157583
REFERENCE   2  (bases 1 to 231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2022-09-3"; volume: "25(10):105054. doi"; pages: " 
            10.1016/j.isci.2022.105054. eCollection 2022 Oct 21"
COMMENT     Sequence Label: pMCSGC-GB1
FEATURES             Location/Qualifiers
     source          1..231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..204
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="KLALNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDDA
                     TKTFTVTE"
     CDS             211..231
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccaagct tgctctgaac
       61 ggtaaaaccc tgaaaggtga aaccaccacc gaagctgttg acgctgctac cgcggaaaaa
      121 gttttcaaac agtacgctaa cgacaacggt gttgacggtg aatggaccta cgacgacgct
      181 accaaaacct tcacggtaac cgaaggtacc gagaacctgt acttccaatc c
//
