LOCUS       sequence-362717-        1100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  knock out KEAP1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Koppula P, Lei G, Zhang Y, Yan Y, Mao C, Kondiparthi L, Shi J, Liu 
            X, Horbath A, Das M, Li W, Poyurovsky MV, Olszewski K, Gan B
  TITLE     A targetable CoQ-FSP1 axis drives ferroptosis- and 
            radiation-resistance in KEAP1 inactive lung cancers.
  JOURNAL   Nat Commun. 2022 Apr 22;13(1):2206. doi: 10.1038/s41467-022-29905-1.
  PUBMED    35459868
REFERENCE   2  (bases 1 to 1100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-04-22"; volume: "13(1):2206. doi"; pages: " 
            10.1038/s41467-022-29905-1"
COMMENT     Sequence Label: lentiCRISPR v2 sgKEAP1-2
FEATURES             Location/Qualifiers
     source          1..1100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     135..154
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     289..309
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    408..997
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(461..481)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(879..890)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cggaatacaa ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat
       61 tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat
      121 tatgttttaa aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg
      181 ctttatatat cttgtggaaa ggacgaaaca ccgcttgtgg gccatgaact ggggtggagg
      241 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
      301 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
      361 cctggtgcat gacccgcaag cccggtgcct gaacgcgtta agtcgacaat caacctctgg
      421 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
      481 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
      541 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
      601 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
      661 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
      721 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
      781 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
      841 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
      901 ttccttcccg cgggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
      961 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gacttagacc atgacttaca
     1021 ggcagctgta gatctagcca ctttttaaaa ggaaaggggg gactgcaggg ctgtccactc
     1081 ctacgagaca agatctgctt
//
