LOCUS       sequence-36273-e        6433 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6433)
  AUTHORS   Schowalter RM, Pastrana DV, Buck CB
  TITLE     Glycosaminoglycans and sialylated glycans sequentially facilitate 
            merkel cell polyomavirus infectious entry.
  JOURNAL   PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28.
  PUBMED    21829355
REFERENCE   2  (bases 1 to 6433)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2011-07"; volume: "7(7)"; pages: "e1002161. Epub 
            2011 Jul 28"
COMMENT     Sequence Label: pwB3b
FEATURES             Location/Qualifiers
     source          1..6433
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(53..73)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        71..368
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      238..373
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    1168..1289
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1179..1198)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1233..1252
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1471..2649
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1702..2640
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2597..2617
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2673..2697
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2704..2713
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3806..3830)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3892..4480
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3945..3965)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4363..4374)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4522..5094
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      5265..5853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5754..5773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        5939..5986
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        5978..5996
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ccctgcaggg cctgaaataa cctctgaaag aggaacttgg ttaggtacct gtggaatgtg
       61 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      121 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
      181 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
      241 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
      301 atttatacag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
      361 ttttttggag gcctagatcg gttcttctcg gactgggacc ataaggtcag taccgtgggg
      421 ttgtaccaac aaagcgggat ggccctcgag ctcttcaatc ccgacgaata ctacgacatc
      481 ctgttccctg gcgtgaacac cttcgtgaac aacatccagt atctggaccc ccgccactgg
      541 ggaccaagcc tgttcgccac aatcagtcaa gccctctggc acgtcatccg cgacgacatc
      601 cccgccatca caagccagga gctccagcgc cgcaccgaaa ggtttttccg cgatagtctc
      661 gcaagattcc tcgaggagac cacatggacc atcgtgaacg cacccgtgaa tttctacaac
      721 tacatccaag actactactc caacctgagt cccatccgcc caagcatggt ccgccaagtc
      781 gccgagcgcg agggcacaca agtgaacttc gggcacacat atcgcatcga cgacgccgat
      841 tccatccagg aggtcacaca gcgcatggaa ctgcgcaaca aggaaaacgt gcacagcggc
      901 gaattcatcg agaagaccat cgcacccggc ggcgccaacc agcgcaccgc cccacaatgg
      961 atgctcccac tcttgctggg gctctatggc accgtgaccc ctgccctgga ggcctacgag
     1021 gacgggccaa atcagaaaaa gcgccgcgtc agtcgcgggt caagtcagaa ggcaaagggc
     1081 acaagggcct ccgcaaagac caccaacaag cgccgctccc ggtcgagccg ctcatgagtg
     1141 ctagcgggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
     1201 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
     1261 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
     1321 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
     1381 aaatcgataa ggatccgggc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     1441 aacagttgcg gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc
     1501 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     1561 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cccttttccc
     1621 gagggtgggg gagaaccgta tataagtgca gtagtcgctg tgaacgttct ttttcgcaac
     1681 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     1741 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     1801 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     1861 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     1921 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     1981 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     2041 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     2101 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     2161 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     2221 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     2281 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     2341 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     2401 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     2461 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     2521 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     2581 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     2641 gtgtcgtgag gaattctcta gagcttgatc aaacaagttt gtacaaaaaa gcaggcttcc
     2701 ggagccacca tggcacccac aaagcgcaag ggcgaatgcc ccggcgccgc cccaaagaaa
     2761 cccaaggagc cagtccaggt ccccaagttg ctgatcaagg gcggcgtgga ggtcctggag
     2821 gtcaagaccg gcgtggacgc catcaccgag gtggagtgtt tcttgaaccc cgagatgggc
     2881 gaccccgaca acgatctgcg cgggtacagc ttgcgcctga ccgccgagac cgcattcgat
     2941 tccgactcac ccgatcgcaa gatgttgcca tgctattcca ccgcccgcat cccactgccc
     3001 aacctcaacg aagacttgac atgcggcaac ctgctgatgt gggaagccgt caccgtgaag
     3061 accgaagtca tcggcatcac ctccatgttg aatctgcacg ccggaagcca aaaggtgcac
     3121 gagaacggcg gcgggaagcc catccagggg tcaaacttcc atttcttcgc cgtcggcggc
     3181 gatccactcg agatgcaagg cgtgttgatg aactatcgca ccaagtatcc cgagggcacc
     3241 gtgacaccca agaacccaac cgcccaaagt caggtcatga acaccgatca caaagcatat
     3301 ctcgataaga ataacgccta ccccgtcgag tgttggatcc ccgatccatc caagaacgag
     3361 aacacccgct acttcggcac ctataccggc ggcgagaacg tcccacccgt gttgcacgtg
     3421 acaaataccg ccacaaccgt cctgctcgac gagcaaggcg tcggcccact ctgcaaggca
     3481 gacagcctct acgtcagcgc cgccgacatc tgcggactgt tcaccaattc aagcggcacc
     3541 cagcaatggc gcgggttgcc ccgctacttc aaaatcaggc tccgcaagcg cagcgtgaag
     3601 aatccctatc caatcagttt cctgctgtcc gatttgatca atcgccgcac acaacgcgtc
     3661 gacggccagc ccatgtacgg catggaaagc caagtcgaag aagtgcgcgt cttcgacggg
     3721 accgagcagt tgcccggcga tcccgacatg atccgctaca tcgatcgcca aggccagctc
     3781 caaaccaaga tggtctgaga ctagtaccca gctttcttgt acaaagtggt tcgatctaga
     3841 atggctagtg gatcccccgg gctgcaggaa ttcgatatca agcttatcga taatcaacct
     3901 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3961 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4021 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4081 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4141 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4201 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4261 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4321 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4381 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4441 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcggcccact
     4501 gctccctaaa cctgagctag cattatccct aatacctgcc accccactct taatcagtgg
     4561 tggaagaacg gtctcagaac tgtttgtttc aattggccat ttaagtttag tagtaaaaga
     4621 ctggttaatg ataacaatgc atcgtaaaac cttcagaagg aaaggagaat gttttgtgga
     4681 ccactttggt tttctttttt gcgtgtggca gttttaagtt attagttttt aaaatcagta
     4741 ctttttaatg gaaacaactt gaccaaaaat ttgtcacaga attttgagac ccattaaaaa
     4801 agttaaatga gaaacctgtg tgttcctttg gtcaacaccg agacatttag gtgaaagaca
     4861 tctaattctg gttttacgaa tctggaaact tcttgaaaat gtaattcttg agttaacact
     4921 tctgggtgga gaatagggtt gttttccccc cacataattg gaaggggaag gaatatcatt
     4981 taaagctatg ggagggtttc tttgattaca acactggaga gaaatgcagc atgttgctga
     5041 ttgcctgtca ctaaaacagg ccaaaaactg agtccttggg ttgcatagaa agcttcatgt
     5101 tgctaaacca atgttaagtg aatctttgga aacaaaatgt ttccaaatta ctgggatgtg
     5161 catgttgaaa cgtgggttaa ttaactagcc atgaccaaaa tcccttaacg tgagttttcg
     5221 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5281 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5341 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5401 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5461 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5521 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5581 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5641 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5701 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5761 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5821 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5881 ttcctggcct tttgctggcc ttttgctcac atgttcttaa ttaaattttt caaaagtagt
     5941 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgactcac tataggaggg
     6001 ccatcatggc caagttgacc agtgctgtcc cagtgctcac agccagggat gtggctggag
     6061 ctgttgagtt ctggactgac aggttggggt tctccagaga ttttgtggag gatgactttg
     6121 caggtgtggt cagagatgat gtcaccctgt tcatctcagc agtccaggac caggtggtgc
     6181 ctgacaacac cctggcttgg gtgtgggtga gaggactgga tgagctgtat gctgagtgga
     6241 gtgaggtggt ctccaccaac ttcagggatg ccagtggccc tgccatgaca gagattggag
     6301 agcagccctg ggggagagag tttgccctga gagacccagc aggcaactgt gtgcactttg
     6361 tggcagagga gcaggactga ggataagaat tgtaacaaaa aaccccgccc cggcggggtt
     6421 ttttgttaat taa
//
