LOCUS       sequence-36274-e        5047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Schowalter RM, Pastrana DV, Buck CB
  TITLE     Glycosaminoglycans and sialylated glycans sequentially facilitate 
            merkel cell polyomavirus infectious entry.
  JOURNAL   PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28.
  PUBMED    21829355
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2011-07"; volume: "7(7)"; pages: "e1002161. Epub 
            2011 Jul 28"
COMMENT     Sequence Label: ph2b
FEATURES             Location/Qualifiers
     source          1..5047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..28)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13..342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      193..328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      367..376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(373..375,376..378,379..1092)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(373..375,376..378,379..1089)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(418..439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(679..698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1026..1047
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1117..1238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1128..1147)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1182..1201
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1439..1650
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1663..1931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    1973..1997
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3092..3116)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3136..3708
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      3879..4467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4368..4387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4553..4600
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4592..4610
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
       61 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      241 tgactaattt tttttattta tacagaggcc gaggccgcct cggcctctga gctattccag
      301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttgggatcca
      361 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      841 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      901 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      961 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1021 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1081 ctgtacaagt aaagcggccg cttcgagcag acatgataag atacattgat gagtttggac
     1141 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1201 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1261 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1321 aatgtggtaa aatcgataag gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc
     1381 gcccttccca acagttgcgg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg
     1441 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     1501 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     1561 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     1621 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     1681 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     1741 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     1801 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     1861 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     1921 cgttacagat ccaagctgtg accggcgcct acgtaagtga tagcttgatc aaacaagttt
     1981 gtacaaaaaa gcaggctgcg gccgcctagg cttttgcata aatgggcgca gctctggcat
     2041 tgctcggaga tctggtcgca tctgtgtccg aagcagccgc tgcaactgga ttcagcgtcg
     2101 ccgagatcgc tgccggcgaa gcagctgccg caatcgaggt ccagatcgca agtctggcca
     2161 ctgtggaagg gatcacctct acaagcgaag ccatcgctgc catcggattg acccctcaaa
     2221 cctacgccgt gatcgctggc gcacctggcg caatcgctgg tttcgccgca ctgatccaga
     2281 ctgtcaccgg aatctcgagc ctcgcccagg tcggatatcg gttcttctcg gactgggacc
     2341 ataaggtcag taccgtgggg ttgtaccaac aaagcgggat ggccctcgag ctcttcaatc
     2401 ccgacgaata ctacgacatc ctgttccctg gcgtgaacac cttcgtgaac aacatccagt
     2461 atctggaccc ccgccactgg ggaccaagcc tgttcgccac aatcagtcaa gccctctggc
     2521 acgtcatccg cgacgacatc cccgccatca caagccagga gctccagcgc cgcaccgaaa
     2581 ggtttttccg cgatagtctc gcaagattcc tcgaggagac cacatggacc atcgtgaacg
     2641 cacccgtgaa tttctacaac tacatccaag actactactc caacctgagt cccatccgcc
     2701 caagcatggt ccgccaagtc gccgagcgcg agggcacaca agtgaacttc gggcacacat
     2761 atcgcatcga cgacgccgat tccatccagg aggtcacaca gcgcatggaa ctgcgcaaca
     2821 aggaaaacgt gcacagcggc gaattcatcg agaagaccat cgcacccggc ggcgccaacc
     2881 agcgcaccgc cccacaatgg atgctcccac tcttgctggg gctctatggc accgtgaccc
     2941 ctgccctgga ggcctacgag gacgggccaa atcagaaaaa gcgccgcgtc agtcgcgggt
     3001 caagtcagaa ggcaaagggc acaagggcct ccgcaaagac caccaacaag cgccgctccc
     3061 ggtcgagccg ctcatgagtg ctagcgggcc cacccagctt tcttgtacaa agtggttcga
     3121 tctagaatgg ctagcattat ccctaatacc tgccacccca ctcttaatca gtggtggaag
     3181 aacggtctca gaactgtttg tttcaattgg ccatttaagt ttagtagtaa aagactggtt
     3241 aatgataaca atgcatcgta aaaccttcag aaggaaagga gaatgttttg tggaccactt
     3301 tggttttctt ttttgcgtgt ggcagtttta agttattagt ttttaaaatc agtacttttt
     3361 aatggaaaca acttgaccaa aaatttgtca cagaattttg agacccatta aaaaagttaa
     3421 atgagaaacc tgtgtgttcc tttggtcaac accgagacat ttaggtgaaa gacatctaat
     3481 tctggtttta cgaatctgga aacttcttga aaatgtaatt cttgagttaa cacttctggg
     3541 tggagaatag ggttgttttc cccccacata attggaaggg gaaggaatat catttaaagc
     3601 tatgggaggg tttctttgat tacaacactg gagagaaatg cagcatgttg ctgattgcct
     3661 gtcactaaaa caggccaaaa actgagtcct tgggttgcat agaaagcttc atgttgctaa
     3721 accaatgtta agtgaatctt tggaaacaaa atgtttccaa attactggga tgtgcatgtt
     3781 gaaacgtggg ttaattaact agccatgacc aaaatccctt aacgtgagtt ttcgttccac
     3841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     3901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     3961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4021 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4501 gccttttgct ggccttttgc tcacatgttc ttaattaaat ttttcaaaag tagttgacaa
     4561 ttaatcatcg gcatagtata tcggcatagt ataatacgac tcactatagg agggccatca
     4621 tggccaagtt gaccagtgct gtcccagtgc tcacagccag ggatgtggct ggagctgttg
     4681 agttctggac tgacaggttg gggttctcca gagattttgt ggaggatgac tttgcaggtg
     4741 tggtcagaga tgatgtcacc ctgttcatct cagcagtcca ggaccaggtg gtgcctgaca
     4801 acaccctggc ttgggtgtgg gtgagaggac tggatgagct gtatgctgag tggagtgagg
     4861 tggtctccac caacttcagg gatgccagtg gccctgccat gacagagatt ggagagcagc
     4921 cctgggggag agagtttgcc ctgagagacc cagcaggcaa ctgtgtgcac tttgtggcag
     4981 aggagcagga ctgaggataa gaattgtaac aaaaaacccc gccccggcgg ggttttttgt
     5041 taattaa
//
