LOCUS       sequence-36277-e        4683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Schowalter RM, Pastrana DV, Buck CB
  TITLE     Glycosaminoglycans and sialylated glycans sequentially facilitate 
            merkel cell polyomavirus infectious entry.
  JOURNAL   PLoS Pathog. 2011 Jul;7(7):e1002161. Epub 2011 Jul 28.
  PUBMED    21829355
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2011-07"; volume: "7(7)"; pages: "e1002161. Epub 
            2011 Jul 28"
COMMENT     Sequence Label: ph3b
FEATURES             Location/Qualifiers
     source          1..4683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(8..28)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13..342
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      193..328
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      367..376
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(373..375,376..378,379..1092)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(373..375,376..378,379..1089)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(418..439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(679..698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1026..1047
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1117..1238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1128..1147)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1182..1201
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1439..1650
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1663..1931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     protein_bind    complement(2728..2752)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    2772..3344
                     /label=EF-1-alpha poly(A) signal
                     /note="human EF-1-alpha polyadenylation signal"
     rep_origin      3515..4103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4004..4023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        4189..4236
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        4228..4246
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cccctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
       61 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
      181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
      241 tgactaattt tttttattta tacagaggcc gaggccgcct cggcctctga gctattccag
      301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttgggatcca
      361 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
      841 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
      901 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
      961 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1021 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1081 ctgtacaagt aaagcggccg cttcgagcag acatgataag atacattgat gagtttggac
     1141 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     1201 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     1261 ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca
     1321 aatgtggtaa aatcgataag gatccgggct ggcgtaatag cgaagaggcc cgcaccgatc
     1381 gcccttccca acagttgcgg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg
     1441 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     1501 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     1561 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     1621 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct
     1681 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg
     1741 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg
     1801 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc
     1861 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc
     1921 cgttacagat ccaagctgtg accggcgcct acatcggttc ttctcggact gggaccataa
     1981 ggtcagtacc gtggggttgt accaacaaag cgggatggcc ctcgagctct tcaatcccga
     2041 cgaatactac gacatcctgt tccctggcgt gaacaccttc gtgaacaaca tccagtatct
     2101 ggacccccgc cactggggac caagcctgtt cgccacaatc agtcaagccc tctggcacgt
     2161 catccgcgac gacatccccg ccatcacaag ccaggagctc cagcgccgca ccgaaaggtt
     2221 tttccgcgat agtctcgcaa gattcctcga ggagaccaca tggaccatcg tgaacgcacc
     2281 cgtgaatttc tacaactaca tccaagacta ctactccaac ctgagtccca tccgcccaag
     2341 catggtccgc caagtcgccg agcgcgaggg cacacaagtg aacttcgggc acacatatcg
     2401 catcgacgac gccgattcca tccaggaggt cacacagcgc atggaactgc gcaacaagga
     2461 aaacgtgcac agcggcgaat tcatcgagaa gaccatcgca cccggcggcg ccaaccagcg
     2521 caccgcccca caatggatgc tcccactctt gctggggctc tatggcaccg tgacccctgc
     2581 cctggaggcc tacgaggacg ggccaaatca gaaaaagcgc cgcgtcagtc gcgggtcaag
     2641 tcagaaggca aagggcacaa gggcctccgc aaagaccacc aacaagcgcc gctcccggtc
     2701 gagccgctca tgagtgctag cgggcccacc cagctttctt gtacaaagtg gttcgatcta
     2761 gaatggctag cattatccct aatacctgcc accccactct taatcagtgg tggaagaacg
     2821 gtctcagaac tgtttgtttc aattggccat ttaagtttag tagtaaaaga ctggttaatg
     2881 ataacaatgc atcgtaaaac cttcagaagg aaaggagaat gttttgtgga ccactttggt
     2941 tttctttttt gcgtgtggca gttttaagtt attagttttt aaaatcagta ctttttaatg
     3001 gaaacaactt gaccaaaaat ttgtcacaga attttgagac ccattaaaaa agttaaatga
     3061 gaaacctgtg tgttcctttg gtcaacaccg agacatttag gtgaaagaca tctaattctg
     3121 gttttacgaa tctggaaact tcttgaaaat gtaattcttg agttaacact tctgggtgga
     3181 gaatagggtt gttttccccc cacataattg gaaggggaag gaatatcatt taaagctatg
     3241 ggagggtttc tttgattaca acactggaga gaaatgcagc atgttgctga ttgcctgtca
     3301 ctaaaacagg ccaaaaactg agtccttggg ttgcatagaa agcttcatgt tgctaaacca
     3361 atgttaagtg aatctttgga aacaaaatgt ttccaaatta ctgggatgtg catgttgaaa
     3421 cgtgggttaa ttaactagcc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3481 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3541 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3601 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3661 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3721 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3781 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3841 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3901 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3961 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     4021 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4081 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4141 tttgctggcc ttttgctcac atgttcttaa ttaaattttt caaaagtagt tgacaattaa
     4201 tcatcggcat agtatatcgg catagtataa tacgactcac tataggaggg ccatcatggc
     4261 caagttgacc agtgctgtcc cagtgctcac agccagggat gtggctggag ctgttgagtt
     4321 ctggactgac aggttggggt tctccagaga ttttgtggag gatgactttg caggtgtggt
     4381 cagagatgat gtcaccctgt tcatctcagc agtccaggac caggtggtgc ctgacaacac
     4441 cctggcttgg gtgtgggtga gaggactgga tgagctgtat gctgagtgga gtgaggtggt
     4501 ctccaccaac ttcagggatg ccagtggccc tgccatgaca gagattggag agcagccctg
     4561 ggggagagag tttgccctga gagacccagc aggcaactgt gtgcactttg tggcagagga
     4621 gcaggactga ggataagaat tgtaacaaaa aaccccgccc cggcggggtt ttttgttaat
     4681 taa
//
