LOCUS       sequence-362770-        8849 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression vector encoding S240A-mutated p115 RhoGEF 
            tagged with mCherry..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8849)
  AUTHORS   Zhang JZ, Nguyen AH, Miyamoto S, Heller Brown J, McCulloch AD, Zhang
            J
  TITLE     Histamine-induced biphasic activation of RhoA allows for persistent 
            RhoA signaling.
  JOURNAL   PLoS Biol. 2020 Sep 3;18(9):e3000866. doi: 
            10.1371/journal.pbio.3000866. eCollection 2020 Sep.
  PUBMED    32881857
REFERENCE   2  (bases 1 to 8849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2020-09-3"; volume: "18(9):e3000866. doi"; pages: " 
            10.1371/journal.pbio.3000866. eCollection 2020 Sep"
COMMENT     Sequence Label: pcDNA3.1(+)-mCherry-p115(S240A)
FEATURES             Location/Qualifiers
     source          1..8849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        11..390
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        391..594
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     545..565
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     639..658
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        639..657
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      665..674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             671..1378
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(816..834)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1084..1103
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(4178..4196)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4179..4196)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4216..4233)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4222..4446
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4492..4920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4579..4598)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4789..4810
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4929..4949)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4934..5263
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5114..5249
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5176..5195
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5330..6124
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5384..5403)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5994..6013
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6298..6419
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6335..6354)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6389..6408
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6468..6484)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6468..6484)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6481..6503)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6492..6508
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6516..6522,6523..6540,6541..6546))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6561..6582
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6699..6716)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6870..7458)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6950..6969)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7629..8420,8421..8489))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8252..8271
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8490..8594)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8669..8688)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
       61 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      121 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      181 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      241 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      301 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      361 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
      421 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
      481 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
      541 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
      601 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
      661 acccgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
      721 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
      781 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
      841 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
      901 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
      961 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     1021 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     1081 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     1141 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     1201 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     1261 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     1321 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagat
     1381 ggaagacttc gcccgagggg cggcctcccc aggcccctcc cggcctggcc tggttcccgt
     1441 cagcatcatc ggggctgagg atgaggattt tgagaacgag ctggagacaa actcagaaga
     1501 gcaaaacagc cagttccaga gcctggagca ggtgaagcgg cgcccagccc acctcatggc
     1561 cctcctgcag cacgtggccc tgcagtttga gccaggaccc ctgctttgct gtctgcatgc
     1621 cgacatgctg ggctcactgg gccccaagga ggccaagaag gccttcctgg acttctacca
     1681 cagcttcctg gagaagacag cggttctccg ggtgccggtc cctcccaacg tcgcctttga
     1741 acttgaccgc actagggctg acctcatctc cgaggatgtc cagcggcggt tcgtgcagga
     1801 ggtggtgcaa agccagcagg tagccgtggg ccggcagctg gaggacttcc gttccaagcg
     1861 gctcatgggc atgacgccct gggagcagga gctggcccag ctggaggctt gggttgggcg
     1921 ggaccgagcc agctacgagg cccgggagcg gcacgtggcg gagcggctgc tcatgcacct
     1981 ggaggagatg caacatacca tctctaccga cgaagaaaag agtgctgccg tggtcaacgc
     2041 cattggcctg tacatgcgcc accttggggt gcggaccaag agtggagaca agaaggcagg
     2101 tagaaatttc ttccggaaaa aggtgatggg gaaccggcgg tcggacgagc ctgccaagac
     2161 caagaagggg ctgagcagca tcctggatgc cgcccgctgg aaccggggag agccccaggt
     2221 tccagatttt cgacacctca aagcagaggt tgatgccgag aagccaggtg ctacagaccg
     2281 gaagggaggc gtggggatgc cctctcggga ccggaatatc ggggctcctg ggcaggacac
     2341 ccctggagtc tctctgcacc ctctgtccct ggacagccca gaccgggaac caggtgctga
     2401 cgcccccctg gagctggggg actcatcccc gcagggccca atgagcctgg agtccttggc
     2461 gcccccagag agtaccgacg agggggccga aaccgagagc cccgagcctg gagatgaggg
     2521 ggagccgggg cggtcgggac tggagcttga accagaagag cctcccggct ggcgggaact
     2581 cgtcccccca gacaccctgc acagcctgcc caagagccag gtgaagcggc aggaggtcat
     2641 cagcgagctg ctggtgacag aggcggccca cgtgcgcatg ctgcgggtgc tgcacgacct
     2701 cttcttccag cccatggcag aatgcctgtt cttccccttg gaggagctgc agaacatctt
     2761 ccccagcctg gacgagctca tcgaggtgca ttccctgttc ctcgatcgcc tgatgaagcg
     2821 gaggcaggag agtggctacc tcatcgagga gatcggagac gtgctgctgg cccggtttga
     2881 tggtgctgag ggctcctggt tccagaaaat ctcctcccgc ttctgcagcc gccagtcatt
     2941 tgccttagag cagctcaaag ccaagcaacg caaggaccct cggttctgtg ccttcgtgca
     3001 ggaagctgag agccgcccgc ggtgccgccg cctgcagctg aaggacatga tccccacgga
     3061 gatgcagcgg ctgaccaagt accccctgct cctgcagagc atcgggcaga acacagaaga
     3121 gcccacagaa cgggagaaag tggagctggc agccgagtgc tgccgggaaa ttctacacca
     3181 cgtcaaccaa gccgtgcgtg acatggagga cctgctgagg ctcaaggact atcagcggcg
     3241 cctggacttg tcccaccttc ggcagagcag cgaccctatg ctgagcgagt tcaagaacct
     3301 ggacatcacc aagaagaaat tggtccacga gggcccactg acgtggcggg tgactaagga
     3361 caaggcagtg gaggtgcatg tgctgctgct ggacgacctg ctgctgctgc tccagcgcca
     3421 ggacgagcgg ctgctgctca agtcccatag ccggacactg acgcccacgc ccgatggcaa
     3481 gaccatgctg cggcccgtgc tgcggctcac ctccgccatg acccgcgagg tggccaccga
     3541 tcacaaagcc ttctacgtcc tttttacctg ggaccaggag gcccagatat acgagctggt
     3601 ggcacagact gtgtcggagc ggaaaaactg gtgtgctctc atcactgaga ctgccggatc
     3661 cctgaaagtc cctgcccctg cctctcgccc taagccccgg cccagcccga gcagcacccg
     3721 agaacccctc ctcagcagct ctgagaacgg caatggtggc cgagagacgt ctccagctga
     3781 tgcccggacc gagagaatcc tcagtgacct cctgcccttc tgcagaccag gccccgaggg
     3841 ccagctcgct gccacggccc ttcggaaagt gctgtccctg aagcagcttc tgtttccggc
     3901 ggaggaagac aatggggcgg ggcctcctcg agatggggat ggggtcccag ggggcggccc
     3961 cctgagccca gcacggaccc aggaaatcca ggagaacctg ctcagcttgg aggagaccat
     4021 gaagcagctg gaggagttgg aggaggaatt ttgccgcctg agacccctcc tgtctcagct
     4081 tggggggaac tctgtccccc agcctggctg cacttgagaa ttctgcagat atccagcaca
     4141 gtggcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
     4201 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     4261 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     4321 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     4381 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     4441 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
     4501 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4561 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4621 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4681 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4741 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4801 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4861 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4921 aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4981 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5041 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5101 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5161 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca
     5221 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg
     5281 tatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
     5341 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
     5401 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
     5461 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aggacgaggc
     5521 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
     5581 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
     5641 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
     5701 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
     5761 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
     5821 gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct
     5881 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
     5941 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
     6001 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
     6061 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
     6121 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga
     6181 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac
     6241 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccccaac
     6301 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     6361 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     6421 catgtctgta taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt
     6481 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     6541 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     6601 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     6661 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     6721 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6781 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6841 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6901 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6961 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     7021 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     7081 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     7141 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagatac
     7201 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     7261 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7321 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7381 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7441 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7501 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7561 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7621 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7681 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7741 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7801 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7861 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7921 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7981 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     8041 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     8101 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     8161 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     8221 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     8281 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8341 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8401 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8461 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8521 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8581 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat
     8641 ctcccgatcc cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     8701 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     8761 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     8821 ttgcgctgct tcgcgatgta cgggccaga
//
