LOCUS       sequence-362785-       11216 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11216)
  AUTHORS   Erdenee E, Ting AY
  TITLE     A Dual-Purpose Real-Time Indicator and Transcriptional Integrator 
            for Calcium Detection in Living Cells.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1086-1095. doi: 
            10.1021/acssynbio.1c00597. Epub 2022 Mar 7.
  PUBMED    35254056
REFERENCE   2  (bases 1 to 11216)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00597"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1086-1095"
COMMENT     Sequence Label: NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1
FEATURES             Location/Qualifiers
     source          1..11216
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(117..136)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        308..687
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        688..891
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     842..862
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     936..955
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        936..954
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      974..983
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             983..1006
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4562..4672
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     misc_feature    4703..5255
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4847..4864)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5074..5093
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5264..5273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5282..5302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5303..5740
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5774..6283
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     CDS             6797..6823
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(6842..6860)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6843..6860)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6880..6897)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6886..7110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7156..7584
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7243..7262)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7453..7474
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7593..7613)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7598..7927
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7778..7913
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7840..7859
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7994..8788
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8048..8067)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8658..8677
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8962..9083
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8999..9018)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9053..9072
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9132..9148)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9132..9148)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9145..9167)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9156..9172
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9180..9186,9187..9204,9205..9210))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9225..9246
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9363..9380)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9534..10122)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9614..9633)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10293..11084,11085..11153))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10916..10935
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11154..11216,1..42))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
       61 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
      121 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
      181 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
      241 atgaagaatc tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat
      301 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      361 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      421 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      481 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      541 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      601 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      661 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
      721 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      781 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
      841 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
      901 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
      961 caagcttggt accgccacca tggattacaa ggacgatgac gataaagggg ctagttcatc
     1021 ttcatctgag agatggatcg atggtcttca gttctcttcc ttgttatggc ctccgccacg
     1081 agatcctcaa caacataagg atcaagtcgt tgcttatgtt gaatattttg gtcaatttac
     1141 atcagagcaa ttcccagatg acattgctga gttggtccgg catcagtatc catcaaccga
     1201 gaagcgactt ttggacgatg tgctggcgat gtttgtcctt catcatccgg agcatggtca
     1261 tgcagtcatt cttccaatca tttcatgtct tattgatggc tcgttggtgt acagcaagga
     1321 agctcatccg tttgcctctt tcatatcttt agtttgccca agtagtgaga atgactattc
     1381 ggagcaatgg gctttggcat gtggagaaat ccttcgcatt ttgactcatt acaaccgtcc
     1441 catttataaa actgagcagc aaaatggaga tacagagaga aattgtctga gcaaagctac
     1501 aactagtggt tctccgactt cagagcctaa ggctggatca ccaacacagc atgaaaggaa
     1561 acctttaagg cctttgtctc catggatcag tgatatacta cttgctgctc ctcttggtat
     1621 aagaagtgac tatttccgat ggtgtagtgg tgtaatgggt aaatatgctg ctggagagct
     1681 caagccgcca accattgctt ctcgaggatc tggtaaacat cctcaactga tgccttcaac
     1741 cccaagatgg gctgttgcta atggagctgg tgtcatactg agtgtttgtg atgatgaagt
     1801 tgctcgatat gagactgcta cgctgacagc ggtcgctgtc cctgcacttc ttcttcctcc
     1861 gccaacgaca tccttagatg agcatctagt tgctggcctt ccagctcttg aaccatatgc
     1921 acgtttgttt catagatact atgccattgc aactccaagt gctacgcaga gacttcttct
     1981 tggactctta gaagcaccac cgtcgtgggc tccagatgca cttgatgctg ctgtacagct
     2041 tgtggaactc cttcgagctg ctgaagatta tgcatctggt gtaaggctac ccaggaactg
     2101 gatgcatttg cacttcttgc gggctatagg aattgctatg tctatgaggg caggtgttgc
     2161 tgctgatgct gcagccgctt tgcttttccg catactctca cagccggcac tgctttttcc
     2221 tccgctaagt caagttgagg gagtagaaat tcagcacgcg cctattggtg gctacagttc
     2281 aaattacaga aaacagatag aagttcctgc agcagaagca accattgaag ccactgccca
     2341 aggaattgcc tcaatgcttt gtgctcatgg tcctgaagtt gagtggagaa tttgcactat
     2401 atgggaagct gcttatggtt tgatcccttt aaattcttcg gcggttgatc ttcccgaaat
     2461 catagttgct accccactgc aacctcctat cttgtcatgg aatttataca ttccactcct
     2521 caaagtactt gaatatcttc cacgggggag tccttcggaa gcatgcttga tgaaaatatt
     2581 tgttgccact gtggaaacaa tactcagtag aacttttccg cctgaatctt ccagggaact
     2641 aaccagaaaa gctagatcga gttttaccac aagatcagcg accaaaaatc ttgctatgtc
     2701 tgagcttcgt gctatggtcc atgctctctt tttagaatca tgcgctggtg tggaattagc
     2761 ttcacgccta ctttttgttg tgttgactgt atgtgttagc catgaagcac agtctagtgg
     2821 tagcaagaga ccgagaagtg aatatgctag tactactgaa aatattgagg cgaatcaacc
     2881 tgtatctaac aatcaaactg ctaaccgtaa aagtaggaat gtcaagggac agggacctgt
     2941 ggcagcattt gattcatacg ttcttgctgc tgtttgtgct cttgcctgtg aggttcagct
     3001 gtatcctatg atctctggtg gggggaactt ttccaattct gccgtggctg gaactattac
     3061 aaagcctgta aagataaatg ggtcatctaa agagtatgga gctgggattg actcggcaat
     3121 tagtcatacg cgccgaattt tggcaatcct agaggcactc ttttcattaa aaccatcttc
     3181 tgtggggact ccatggagtt acagttctag tgagatagtt gctgcggcca tggttgcagc
     3241 tcatatttcc gaactgttca gacgttcaaa ggccttgacg catgcattgt ctgggttgat
     3301 gagatgtaag tgggataagg aaattcataa aagagcatca tcattatata acctcataga
     3361 tgttcacagc aaagttgttg cctccattgt tgacaaagct gaacccttgg aagcctacct
     3421 taagaataca ccggttcaga aggattctgt gacctgttta aactggaaac aagagaacac
     3481 atgtgcaagc accacatgct ttgatacagc ggtgacatcc gcctcaagga ctgaaatgaa
     3541 tccaagagga aaccataagt atgctagaca ttcagatgaa ggctcaggaa gaccctcaga
     3601 gaagggtatc aaagatttcc tcttggatgc ttctgatcta gcgaatttcc tcacagctga
     3661 tagactcgca gggttctatt gtggtacaca aaagcttttg aggtcagtgc ttgcagagaa
     3721 accggagctg tctttctccg ttgtttcact gttatggcac aaactgattg ctgctcctga
     3781 aatccagccc accgcagaaa gcacctctgc gcaacaagga tggagacagg ttgttgatgc
     3841 gctatgcaat gtcgtatctg caacgccagc gaaagcagca gcagcagttg tccttcaggc
     3901 tgaaagggag ttgcagcctt ggatcgccaa agatgatgaa gaaggccaaa aaatgtggaa
     3961 aatcaaccaa cggatagtca aagtgttggt ggaactcatg cgcaatcatg acaggcctga
     4021 gtcactggtg attctcgcaa gtgcatcaga tcttcttctg cgggcaactg atggaatgct
     4081 tgttgatgga gaagcttgta cattacctca acttgagcta cttgaagcca cggcaagagc
     4141 aatacagccg gtgctagctt gggggccatc tggactagca gtggtcgacg gtttatccaa
     4201 tctattgaag tgtcgtctac cagcaacaat acggtgcctt tcacacccaa gtgcacacgt
     4261 acgtgcctta agcacgtcag tactacgtga tatcatgaac caaagctcca tacccatcaa
     4321 agtaactcca aaactgccaa caacagagaa gaacggaatg aatagtccgt cctatcgatt
     4381 cttcaacgcc gcctcaatag actggaaagc cgatatccaa aactgtttaa actgggaagc
     4441 tcacagcttg ctctccacaa ctatgcctac tcagtttctc gacactgcgg ctcgggaact
     4501 cggctgtact atatccttgt cccaagcggc cgcaagtgct ggtgaaagcg gcgggccggc
     4561 cgacgccctt gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga
     4621 ccttgatatg ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggtt
     4681 aattaactag cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     4741 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4801 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4861 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4921 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     4981 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5041 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     5101 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     5161 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     5221 gacgtggttt tcctttgaaa aacacgatga taatatggcc acagccacca tgggcgcgcc
     5281 gcccaagaag aagaggaagg tgaagctact gtcttctatc gaacaagcat gcgatatttg
     5341 ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa
     5401 caactgggag tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct
     5461 gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
     5521 agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
     5581 aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt
     5641 ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga
     5701 agagagtagt aacaaaggtc aaagacagtt gactgtatcg ccgggcgacc cactagtcca
     5761 gtgtggtgga attgtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg
     5821 ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg
     5881 ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga
     5941 catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa
     6001 aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg
     6061 cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga
     6121 aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa
     6181 caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat
     6241 caacggagtg accggctggc ggctgtgcga acgcattctg gcggcggccg cagcgagaga
     6301 acatgcgatc ggagaagcca ccggaaaacg aaagaaacgc ggcagagttg aagaagcaga
     6361 agaatactgt aacgatggaa tcgaagaaca agtagaggat gagaagcttc cgttagaggt
     6421 tgggatgttc tattacccaa tgactccgcc ttcgttcatt gtttccgatg ctctggagcc
     6481 agattttcct ttgatctatg tcaacagagt cttcgaagtc ttcactggct atcgtgccga
     6541 tgaagttctt ggtcgtaact gtcgattcct acagtacaga gatcctcgag ctcaaaggcg
     6601 tctcccattg gttgatcctg tggttgtatc tgagattagg agatgtcttg aagaaggtat
     6661 tgaattccaa ggagagcttc ttaatttcag aaaagatggt actcctttgg ttaacagact
     6721 acggcttgct ccaatacgtg acgatgatgg aaccattaca cacgtaattg ggatacaggt
     6781 cttctctgaa acggcttacc catacgatgt tccagattac gcttagtcta gagggcccta
     6841 ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt
     6901 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     6961 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     7021 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     7081 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     7141 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     7201 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     7261 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     7321 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     7381 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     7441 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     7501 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     7561 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     7621 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     7681 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7741 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     7801 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     7861 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     7921 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
     7981 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     8041 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     8101 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     8161 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     8221 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     8281 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     8341 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     8401 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     8461 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     8521 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     8581 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     8641 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     8701 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     8761 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     8821 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     8881 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     8941 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
     9001 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     9061 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
     9121 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     9181 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     9241 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9301 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     9361 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     9421 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     9481 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     9541 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     9601 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     9661 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     9721 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     9781 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     9841 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     9901 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     9961 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    10021 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    10081 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    10141 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    10201 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    10261 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    10321 atctcagcga tctgtctatt tcgttcatcc atagttgcct gacttcccgt cgtgtagata
    10381 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    10441 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    10501 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    10561 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    10621 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    10681 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    10741 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    10801 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    10861 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    10921 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    10981 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    11041 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    11101 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    11161 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacat
//
