LOCUS       sequence-362796-       10937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the 
            nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10937)
  AUTHORS   Erdenee E, Ting AY
  TITLE     A Dual-Purpose Real-Time Indicator and Transcriptional Integrator 
            for Calcium Detection in Living Cells.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1086-1095. doi: 
            10.1021/acssynbio.1c00597. Epub 2022 Mar 7.
  PUBMED    35254056
REFERENCE   2  (bases 1 to 10937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00597"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1086-1095"
COMMENT     Sequence Label: NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1
FEATURES             Location/Qualifiers
     source          1..10937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             919..939
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             940..963
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4501..4650
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             4657..4698
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4723..5275
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4867..4884)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5094..5113
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5284..5293
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5302..5322
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5323..5760
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             5794..5850
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     CDS             6445..6471
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(6490..6508)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6491..6508)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6528..6545)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6534..6758
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6804..7232
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6891..6910)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7101..7122
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7241..7261)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7246..7575
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7426..7561
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7488..7507
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             7642..8436
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7696..7715)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8306..8325
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    8610..8731
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8647..8666)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8701..8720
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8780..8796)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8780..8796)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8793..8815)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8804..8820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8828..8834,8835..8852,8853..8858))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8873..8894
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9011..9028)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9182..9770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9262..9281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9941..10732,10733..10801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10564..10583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10802..10906)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gccaccatgg gcgcgccgcc caagaagaag aggaaggtgg actacaagga cgatgacgat
      961 aaaggggcta gttcatcttc atctgagaga tggatcgatg gtcttcagtt ctcttccttg
     1021 ttatggcctc cgccacgaga tcctcaacaa cataaggatc aagtcgttgc ttatgttgaa
     1081 tattttggtc aatttacatc agagcaattc ccagatgaca ttgctgagtt ggtccggcat
     1141 cagtatccat caaccgagaa gcgacttttg gacgatgtgc tggcgatgtt tgtccttcat
     1201 catccggagc atggtcatgc agtcattctt ccaatcattt catgtcttat tgatggctcg
     1261 ttggtgtaca gcaaggaagc tcatccgttt gcctctttca tatctttagt ttgcccaagt
     1321 agtgagaatg actattcgga gcaatgggct ttggcatgtg gagaaatcct tcgcattttg
     1381 actcattaca accgtcccat ttataaaact gagcagcaaa atggagatac agagagaaat
     1441 tgtctgagca aagctacaac tagtggttct ccgacttcag agcctaaggc tggatcacca
     1501 acacagcatg aaaggaaacc tttaaggcct ttgtctccat ggatcagtga tatactactt
     1561 gctgctcctc ttggtataag aagtgactat ttccgatggt gtagtggtgt aatgggtaaa
     1621 tatgctgctg gagagctcaa gccgccaacc attgcttctc gaggatctgg taaacatcct
     1681 caactgatgc cttcaacccc aagatgggct gttgctaatg gagctggtgt catactgagt
     1741 gtttgtgatg atgaagttgc tcgatatgag actgctacgc tgacagcggt cgctgtccct
     1801 gcacttcttc ttcctccgcc aacgacatcc ttagatgagc atctagttgc tggccttcca
     1861 gctcttgaac catatgcacg tttgtttcat agatactatg ccattgcaac tccaagtgct
     1921 acgcagagac ttcttcttgg actcttagaa gcaccaccgt cgtgggctcc agatgcactt
     1981 gatgctgctg tacagcttgt ggaactcctt cgagctgctg aagattatgc atctggtgta
     2041 aggctaccca ggaactggat gcatttgcac ttcttgcggg ctataggaat tgctatgtct
     2101 atgagggcag gtgttgctgc tgatgctgca gccgctttgc ttttccgcat actctcacag
     2161 ccggcactgc tttttcctcc gctaagtcaa gttgagggag tagaaattca gcacgcgcct
     2221 attggtggct acagttcaaa ttacagaaaa cagatagaag ttcctgcagc agaagcaacc
     2281 attgaagcca ctgcccaagg aattgcctca atgctttgtg ctcatggtcc tgaagttgag
     2341 tggagaattt gcactatatg ggaagctgct tatggtttga tccctttaaa ttcttcggcg
     2401 gttgatcttc ccgaaatcat agttgctacc ccactgcaac ctcctatctt gtcatggaat
     2461 ttatacattc cactcctcaa agtacttgaa tatcttccac gggggagtcc ttcggaagca
     2521 tgcttgatga aaatatttgt tgccactgtg gaaacaatac tcagtagaac ttttccgcct
     2581 gaatcttcca gggaactaac cagaaaagct agatcgagtt ttaccacaag atcagcgacc
     2641 aaaaatcttg ctatgtctga gcttcgtgct atggtccatg ctctcttttt agaatcatgc
     2701 gctggtgtgg aattagcttc acgcctactt tttgttgtgt tgactgtatg tgttagccat
     2761 gaagcacagt ctagtggtag caagagaccg agaagtgaat atgctagtac tactgaaaat
     2821 attgaggcga atcaacctgt atctaacaat caaactgcta accgtaaaag taggaatgtc
     2881 aagggacagg gacctgtggc agcatttgat tcatacgttc ttgctgctgt ttgtgctctt
     2941 gcctgtgagg ttcagctgta tcctatgatc tctggtgggg ggaacttttc caattctgcc
     3001 gtggctggaa ctattacaaa gcctgtaaag ataaatgggt catctaaaga gtatggagct
     3061 gggattgact cggcaattag tcatacgcgc cgaattttgg caatcctaga ggcactcttt
     3121 tcattaaaac catcttctgt ggggactcca tggagttaca gttctagtga gatagttgct
     3181 gcggccatgg ttgcagctca tatttccgaa ctgttcagac gttcaaaggc cttgacgcat
     3241 gcattgtctg ggttgatgag atgtaagtgg gataaggaaa ttcataaaag agcatcatca
     3301 ttatataacc tcatagatgt tcacagcaaa gttgttgcct ccattgttga caaagctgaa
     3361 cccttggaag cctaccttaa gaatacaccg gttcagaagg attctgtgac ctgtttaaac
     3421 tggaaacaag agaacacatg tgcaagcacc acatgctttg atacagcggt gacatccgcc
     3481 tcaaggactg aaatgaatcc aagaggaaac cataagtatg ctagacattc agatgaaggc
     3541 tcaggaagac cctcagagaa gggtatcaaa gatttcctct tggatgcttc tgatctagcg
     3601 aatttcctca cagctgatag actcgcaggg ttctattgtg gtacacaaaa gcttttgagg
     3661 tcagtgcttg cagagaaacc ggagctgtct ttctccgttg tttcactgtt atggcacaaa
     3721 ctgattgctg ctcctgaaat ccagcccacc gcagaaagca cctctgcgca acaaggatgg
     3781 agacaggttg ttgatgcgct atgcaatgtc gtatctgcaa cgccagcgaa agcagcagca
     3841 gcagttgtcc ttcaggctga aagggagttg cagccttgga tcgccaaaga tgatgaagaa
     3901 ggccaaaaaa tgtggaaaat caaccaacgg atagtcaaag tgttggtgga actcatgcgc
     3961 aatcatgaca ggcctgagtc actggtgatt ctcgcaagtg catcagatct tcttctgcgg
     4021 gcaactgatg gaatgcttgt tgatggagaa gcttgtacat tacctcaact tgagctactt
     4081 gaagccacgg caagagcaat acagccggtg ctagcttggg ggccatctgg actagcagtg
     4141 gtcgacggtt tatccaatct attgaagtgt cgtctaccag caacaatacg gtgcctttca
     4201 cacccaagtg cacacgtacg tgccttaagc acgtcagtac tacgtgatat catgaaccaa
     4261 agctccatac ccatcaaagt aactccaaaa ctgccaacaa cagagaagaa cggaatgaat
     4321 agtccgtcct atcgattctt caacgccgcc tcaatagact ggaaagccga tatccaaaac
     4381 tgtttaaact gggaagctca cagcttgctc tccacaacta tgcctactca gtttctcgac
     4441 actgcggctc gggaactcgg ctgtactata tccttgtccc aagcggccgc aagtgctggt
     4501 gatgctttag acgattttga cttagatatg cttggttcag acgcgttaga cgacttcgac
     4561 ctagacatgt taggctcaga tgcattggac gacttcgatt tagatatgtt gggctccgat
     4621 gccctagatg actttgatct agatatgcta ggtagtggca agcccatccc caaccccctg
     4681 ctgggcctgg acagcacctt aattaactag cccccccccc taacgttact ggccgaagcc
     4741 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     4801 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     4861 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     4921 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     4981 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     5041 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     5101 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     5161 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     5221 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     5281 acagccacca tgggcgcgcc gcccaagaag aagaggaagg tgaagctact gtcttctatc
     5341 gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag
     5401 tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac caaaaggtct
     5461 ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact ggaacagcta
     5521 tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga ttctttacag
     5581 gatataaaag cattgttaac aggattattt gtacaagata atgtgaataa agatgccgtc
     5641 acagatagat tggcttcagt ggagactgat atgcctctaa cattgagaca gcatagaata
     5701 agtgcgacat catcatcgga agagagtagt aacaaaggtc aaagacagtt gactgtatcg
     5761 ccgggcgacc cactagtcca gtgtggtgga attcgtcgta agtggaataa gacaggtcac
     5821 gcagtcagag ctataggtcg gctgagctca agcggcagcg agactcccgg gacctcagag
     5881 tccgccacac ccgaaagtgt cactggttat agactctttg aagagatact ggcggccgca
     5941 gcgagagaac atgcgatcgg agaagccacc ggaaaacgaa agaaacgcgg cagagttgaa
     6001 gaagcagaag aatactgtaa cgatggaatc gaagaacaag tagaggatga gaagcttccg
     6061 ttagaggttg ggatgttcta ttacccaatg actccgcctt cgttcattgt ttccgatgct
     6121 ctggagccag attttccttt gatctatgtc aacagagtct tcgaagtctt cactggctat
     6181 cgtgccgatg aagttcttgg tcgtaactgt cgattcctac agtacagaga tcctcgagct
     6241 caaaggcgtc tcccattggt tgatcctgtg gttgtatctg agattaggag atgtcttgaa
     6301 gaaggtattg aattccaagg agagcttctt aatttcagaa aagatggtac tcctttggtt
     6361 aacagactac ggcttgctcc aatacgtgac gatgatggaa ccattacaca cgtaattggg
     6421 atacaggtct tctctgaaac ggcttaccca tacgatgttc cagattacgc ttagtctaga
     6481 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
     6541 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     6601 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     6661 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     6721 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     6781 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6841 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6901 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6961 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7021 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7081 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7141 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7201 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     7261 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7321 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7381 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7441 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7501 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7561 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcaagag
     7621 acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc
     7681 gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat
     7741 gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg
     7801 tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct ggccacgacg
     7861 ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta
     7921 ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta
     7981 tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc
     8041 gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc
     8101 gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg
     8161 ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg
     8221 ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt
     8281 gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc
     8341 ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc
     8401 atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga
     8461 ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg
     8521 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     8581 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     8641 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     8701 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     8761 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     8821 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     8881 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     8941 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     9001 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     9061 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     9121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     9181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     9241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     9301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     9361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     9421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     9481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     9661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga cttcccgtcg
    10021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
    10081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
    10141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
    10201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
    10261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
    10321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
    10381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
    10441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
    10501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
    10561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10921 aagtgccacc tgacgtc
//
