LOCUS       sequence-362799-        8085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Ponzio TA, Fields RL, Rashid OM, Salinas YD, Lubelski D, Gainer H
  TITLE     Cell-Type Specific Expression of the Vasopressin Gene Analyzed by 
            AAV Mediated Gene Delivery of Promoter Deletion Constructs into the 
            Rat SON In Vivo.
  JOURNAL   PLoS One. 2012;7(11):e48860. doi: 10.1371/journal.pone.0048860. Epub
            2012 Nov 14.
  PUBMED    23155418
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(11):e48860. doi"; pages: " 
            10.1371/journal.pone.0048860. Epub 2012 Nov 14"
COMMENT     Sequence Label: p2.0VPI.EGFP
FEATURES             Location/Qualifiers
     source          1..8085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     mobile_element  198..422
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(489..1022)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1211..1216,1217..1233,1234..1239))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     repeat_region   1579..1708
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      3895..3904
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3901..3903,3904..3906,3907..4620)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3901..3903,3904..3906,3907..4617)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3946..3967)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4207..4226)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4554..4575
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     repeat_region   4955..5095
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4955..5084
                     /label=AAV2 ITR
     polyA_signal    5262..5396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5299..5318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5353..5372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  5425..5590
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      5774..6228
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5861..5880)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6070..6091
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6254..6358
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6359..6427,6428..7219)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6577..6596)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7390..7978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7879..7898
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
       61 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      121 acaccgcata gaccagccgc gtaacctggc aaaatcggtt acggttgagt aataaatgga
      181 tgccctgcgt aagcgggtgt gggcggacaa taaagtctta aactgaacaa aatagatcta
      241 aactatgaca ataaagtctt aaactagaca gaatagttgt aaactgaaat cagtccagtt
      301 atgctgtgaa aaagcatact ggacttttgt tatggctaaa gcaaactctt cattttctga
      361 agtgcaaatt gcccgtcgta ttaaagaggg gcgtggccaa gggcatggta aagactatat
      421 tcgcggcgtt gtgacaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga
      481 acgaattgtt aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg
      541 cccaactttg tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc
      601 acataagcac caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg
      661 ccctgcctcc ggtgctcgcc ggagactgcg agatcataga tatagatctc actacgcggc
      721 tgctcaaact tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag
      781 gcagcaagcg cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc
      841 tgatgttggg agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc
      901 cgcatggatt tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag
      961 ccacctaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac
     1021 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
     1081 tgcccgaggc atagactgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     1141 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     1201 cttgcattac agtttacgaa ccgaacaggc ttatgtcaac tgggttcgtg ccttcatccg
     1261 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc
     1321 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt
     1381 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag
     1441 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
     1501 cggttttctg gaaggcgagc atcgtttgtt cgcccaggac tctagctata gttctagtgg
     1561 ttggctacag cttgcatgcc tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc
     1621 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
     1681 gtggccaact ccatcactag gggttccttg tagttaatga ttaacccgcc atgctactta
     1741 tctacgtagc catgctctag agatcagaca atagtctgcc ccaacgctca tcttcagtcc
     1801 attagtggaa aaaaggtttc ttctgaccaa ggaagctgtt tcctctggaa aacaatcact
     1861 taacaaggac attcataaca caaagccgct gtcagctaac atcaccatga cgcaagcact
     1921 tcccttgggg ttcatagtaa gtgactcagg gctcacagcc ctgcagagtg cttgcccttc
     1981 actactttat gctggagtta agtggggagt cagacacccc agatgacctg cccaagtcag
     2041 aaagcttcga ggcatagaag ccagcatgtc ctcggccttc ctacatagca gctgcctctt
     2101 atcctcaagt gccacccagt cattcctgaa aaggtctggc cgccactcct gaggatggac
     2161 gggcagcttg gctccctgtc cccactgtac ttgttgggaa gaggccaaac agtcctctgg
     2221 agctcttgaa gcatgctggt gtacccggga ggggtggcaa gagccgatgg ggatgggagg
     2281 gccctggcct atgcatggtc tagaagccgt gggctaggtg tgcataggtc aagcataggc
     2341 caactaatct gggccccaaa ccataaagtt tttctggtgc ctgaagtaaa ggagtctgta
     2401 aagcctagag cagaatgcgg tgatgcacgc ctgtaaccct agcactggag aggcagagac
     2461 aaaaagatga agagctcaaa gccagctttc agctgtgtag taagttcaag gccagcctgg
     2521 actaaactag actgccttag aaacaaacaa ctgacttaca gtctaaatca ggaattacac
     2581 cagctttctc agactggctc tgtttagctg ggtctcctcc catttcctgt cctagaataa
     2641 cacccacttc caatcctgcc ttagacctga gattttccca acctcagggc ctggggtctc
     2701 cccaaagctc tttcctcttt acggctgtgg gtctcaccaa ggactatttc tgagctctat
     2761 cctacaccct agcttctacc ctagaaggcc tggaactcac agaaactctc ctgcctctgc
     2821 ttcccaatgg ctgggactaa aggcacgcgc cacgactggc cttcttttta tgtttttaat
     2881 attgagacag tgtctcacca agttactcag tctggccttg aacttttgac ctttctactt
     2941 tagcttccca agtggctctg accacaggcc catgaagcca gatcctctgt gcaagctttg
     3001 caccttactg agcctggagt tgacattgcc accatagctt tcccatttgt gtccttagta
     3061 gaaacaatgg gcactattcc caagctctcc ccacccccgg ctctacaggg ttatgcatgg
     3121 gatagaaaca tcctgggtgc ccccgaaggc agccatgcct ggcacagggc agacctttca
     3181 ctgtgttcag ccttgactcc atgactgtgg ccgttagccc atgagactgc aagtgggaat
     3241 ttctttctaa agcccacctg atatgggtgc ttcctctcat cctataccac aactaacaaa
     3301 cctgcccacc ccttctggtg ctgaccctgc tccaaacgtg ccagcccttg ctggatgggg
     3361 cctgtggacc cctttagtct gctgagagca gctggaatat tcaactatga tttccaggtg
     3421 accctccagt cggctcacct cactgatcgc acagcaccaa tcactgtggc agtggctcct
     3481 gtcacacggt ggccggtgac agcctgatgg ctggctcccc tcctccacca ccctctgcac
     3541 tgacaggccc acgtgtgtcc ccagatgcct gaatcactgc tgacagcttg ggacctgtcg
     3601 gctgagggct cctggggagc cactggggag ggggttagca gccacgttgt cgcctcctag
     3661 ggaacacctg cagacataaa taggcagccc agcccgccaa ggcagcagag cctgagctgc
     3721 acacagtgcc cacctatgct cgccaggatg ctcaacacta cgctctccgc ttgtttcctg
     3781 agcctgctgg ccttctcctc cgcctgctac ttccagaact gcccaagagg cggcaagagg
     3841 gccatctctg acatggagct gagacaggta ccgcgggccc gggatccacc ggtcgccacc
     3901 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     3961 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4021 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4081 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4141 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4201 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4261 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4321 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     4381 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     4441 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4501 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4561 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     4621 agcggccgca actactgagc catcgccccc acgcctcgcc cctacagcat ggaaaataaa
     4681 cttttaaaaa ctgcaccctg gtgtctgtct ctatttctgg ggtggggaga agaggggagg
     4741 gagaggcgtt ggagtgggaa ctttctactc tgccctggct aacccccaca cccgaagtcg
     4801 tgcatagtcc acgccccctt ccccaccaga aggggcagaa cctgtaagtc ttatagaata
     4861 aaggaagcgc tagtggccac gtgggccgtg caccttaagc gtctagagca tggctacgta
     4921 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc
     4981 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     5041 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggtctga
     5101 gacaataacc ctgataaatg cttcaataat gtaagcttgt cgagaagtac tagaggatca
     5161 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     5221 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     5281 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5341 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatctgat
     5401 cactgatatc gcctaggaga tccgaaccag ataagtgaaa tctagttcca aactattttg
     5461 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
     5521 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
     5581 tccgcccaca gcggggcatt tttcttcctg ttatgttttt aatcaaacat cctgccaact
     5641 ccatgtgaca aaccgtcatc ttcggctact ttttctctgt cacagaatga aaatttttct
     5701 gtcatctctt cgttattaat gtttgtaatt gactgaatat caacgcttat ttgcagcctg
     5761 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     5821 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     5881 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     5941 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6001 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6061 ttcttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6121 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6181 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt
     6241 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6301 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     6361 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6421 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6481 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6541 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6601 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6661 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6721 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6781 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6841 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6901 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6961 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     7021 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     7081 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     7141 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     7201 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     7261 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7321 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7381 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7441 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7501 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7561 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7621 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7681 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7741 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7801 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7861 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7921 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7981 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     8041 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgc
//
