LOCUS       sequence-362804-       11891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual expression of GI-VP16 and GAL4DBD-GID-SmBiT-FKF1 in the 
            nucleus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11891)
  AUTHORS   Erdenee E, Ting AY
  TITLE     A Dual-Purpose Real-Time Indicator and Transcriptional Integrator 
            for Calcium Detection in Living Cells.
  JOURNAL   ACS Synth Biol. 2022 Mar 18;11(3):1086-1095. doi: 
            10.1021/acssynbio.1c00597. Epub 2022 Mar 7.
  PUBMED    35254056
REFERENCE   2  (bases 1 to 11891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.1c00597"; journalName: "ACS Synth Biol"; date: 
            "2022-03-18- 18"; volume: "11"; issue: "3"; pages: "1086-1095"
COMMENT     Sequence Label: NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1
FEATURES             Location/Qualifiers
     source          1..11891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..64)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        236..615
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..819
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     770..790
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     864..883
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        864..882
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      902..911
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             920..940
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             941..964
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4520..4630
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     CDS             4637..4678
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4703..5255
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4847..4864)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5074..5093
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     regulatory      5264..5273
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5282..5302
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5303..5740
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             7400..7426
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(7445..7463)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(7446..7463)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7483..7500)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7489..7713
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7759..8187
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7846..7865)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8056..8077
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8196..8216)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8201..8530
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8381..8516
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8443..8462
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             8597..9391
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8651..8670)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9261..9280
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9565..9686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9602..9621)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9656..9675
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9735..9751)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9735..9751)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9748..9770)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9759..9775
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9783..9789,9790..9807,9808..9813))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9828..9849
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9966..9983)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10137..10725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10217..10236)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10896..11687,11688..11756))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11519..11538
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11757..11861)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat
       61 gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc
      121 gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg
      181 cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat
      241 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
      301 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      361 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
      421 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      481 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      541 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      601 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
      661 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
      721 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
      781 ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc
      841 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
      901 cgccaccatg ggcgcgccgc ccaagaagaa gaggaaggtg gactacaagg acgatgacga
      961 taaaggggct agttcatctt catctgagag atggatcgat ggtcttcagt tctcttcctt
     1021 gttatggcct ccgccacgag atcctcaaca acataaggat caagtcgttg cttatgttga
     1081 atattttggt caatttacat cagagcaatt cccagatgac attgctgagt tggtccggca
     1141 tcagtatcca tcaaccgaga agcgactttt ggacgatgtg ctggcgatgt ttgtccttca
     1201 tcatccggag catggtcatg cagtcattct tccaatcatt tcatgtctta ttgatggctc
     1261 gttggtgtac agcaaggaag ctcatccgtt tgcctctttc atatctttag tttgcccaag
     1321 tagtgagaat gactattcgg agcaatgggc tttggcatgt ggagaaatcc ttcgcatttt
     1381 gactcattac aaccgtccca tttataaaac tgagcagcaa aatggagata cagagagaaa
     1441 ttgtctgagc aaagctacaa ctagtggttc tccgacttca gagcctaagg ctggatcacc
     1501 aacacagcat gaaaggaaac ctttaaggcc tttgtctcca tggatcagtg atatactact
     1561 tgctgctcct cttggtataa gaagtgacta tttccgatgg tgtagtggtg taatgggtaa
     1621 atatgctgct ggagagctca agccgccaac cattgcttct cgaggatctg gtaaacatcc
     1681 tcaactgatg ccttcaaccc caagatgggc tgttgctaat ggagctggtg tcatactgag
     1741 tgtttgtgat gatgaagttg ctcgatatga gactgctacg ctgacagcgg tcgctgtccc
     1801 tgcacttctt cttcctccgc caacgacatc cttagatgag catctagttg ctggccttcc
     1861 agctcttgaa ccatatgcac gtttgtttca tagatactat gccattgcaa ctccaagtgc
     1921 tacgcagaga cttcttcttg gactcttaga agcaccaccg tcgtgggctc cagatgcact
     1981 tgatgctgct gtacagcttg tggaactcct tcgagctgct gaagattatg catctggtgt
     2041 aaggctaccc aggaactgga tgcatttgca cttcttgcgg gctataggaa ttgctatgtc
     2101 tatgagggca ggtgttgctg ctgatgctgc agccgctttg cttttccgca tactctcaca
     2161 gccggcactg ctttttcctc cgctaagtca agttgaggga gtagaaattc agcacgcgcc
     2221 tattggtggc tacagttcaa attacagaaa acagatagaa gttcctgcag cagaagcaac
     2281 cattgaagcc actgcccaag gaattgcctc aatgctttgt gctcatggtc ctgaagttga
     2341 gtggagaatt tgcactatat gggaagctgc ttatggtttg atccctttaa attcttcggc
     2401 ggttgatctt cccgaaatca tagttgctac cccactgcaa cctcctatct tgtcatggaa
     2461 tttatacatt ccactcctca aagtacttga atatcttcca cgggggagtc cttcggaagc
     2521 atgcttgatg aaaatatttg ttgccactgt ggaaacaata ctcagtagaa cttttccgcc
     2581 tgaatcttcc agggaactaa ccagaaaagc tagatcgagt tttaccacaa gatcagcgac
     2641 caaaaatctt gctatgtctg agcttcgtgc tatggtccat gctctctttt tagaatcatg
     2701 cgctggtgtg gaattagctt cacgcctact ttttgttgtg ttgactgtat gtgttagcca
     2761 tgaagcacag tctagtggta gcaagagacc gagaagtgaa tatgctagta ctactgaaaa
     2821 tattgaggcg aatcaacctg tatctaacaa tcaaactgct aaccgtaaaa gtaggaatgt
     2881 caagggacag ggacctgtgg cagcatttga ttcatacgtt cttgctgctg tttgtgctct
     2941 tgcctgtgag gttcagctgt atcctatgat ctctggtggg gggaactttt ccaattctgc
     3001 cgtggctgga actattacaa agcctgtaaa gataaatggg tcatctaaag agtatggagc
     3061 tgggattgac tcggcaatta gtcatacgcg ccgaattttg gcaatcctag aggcactctt
     3121 ttcattaaaa ccatcttctg tggggactcc atggagttac agttctagtg agatagttgc
     3181 tgcggccatg gttgcagctc atatttccga actgttcaga cgttcaaagg ccttgacgca
     3241 tgcattgtct gggttgatga gatgtaagtg ggataaggaa attcataaaa gagcatcatc
     3301 attatataac ctcatagatg ttcacagcaa agttgttgcc tccattgttg acaaagctga
     3361 acccttggaa gcctacctta agaatacacc ggttcagaag gattctgtga cctgtttaaa
     3421 ctggaaacaa gagaacacat gtgcaagcac cacatgcttt gatacagcgg tgacatccgc
     3481 ctcaaggact gaaatgaatc caagaggaaa ccataagtat gctagacatt cagatgaagg
     3541 ctcaggaaga ccctcagaga agggtatcaa agatttcctc ttggatgctt ctgatctagc
     3601 gaatttcctc acagctgata gactcgcagg gttctattgt ggtacacaaa agcttttgag
     3661 gtcagtgctt gcagagaaac cggagctgtc tttctccgtt gtttcactgt tatggcacaa
     3721 actgattgct gctcctgaaa tccagcccac cgcagaaagc acctctgcgc aacaaggatg
     3781 gagacaggtt gttgatgcgc tatgcaatgt cgtatctgca acgccagcga aagcagcagc
     3841 agcagttgtc cttcaggctg aaagggagtt gcagccttgg atcgccaaag atgatgaaga
     3901 aggccaaaaa atgtggaaaa tcaaccaacg gatagtcaaa gtgttggtgg aactcatgcg
     3961 caatcatgac aggcctgagt cactggtgat tctcgcaagt gcatcagatc ttcttctgcg
     4021 ggcaactgat ggaatgcttg ttgatggaga agcttgtaca ttacctcaac ttgagctact
     4081 tgaagccacg gcaagagcaa tacagccggt gctagcttgg gggccatctg gactagcagt
     4141 ggtcgacggt ttatccaatc tattgaagtg tcgtctacca gcaacaatac ggtgcctttc
     4201 acacccaagt gcacacgtac gtgccttaag cacgtcagta ctacgtgata tcatgaacca
     4261 aagctccata cccatcaaag taactccaaa actgccaaca acagagaaga acggaatgaa
     4321 tagtccgtcc tatcgattct tcaacgccgc ctcaatagac tggaaagccg atatccaaaa
     4381 ctgtttaaac tgggaagctc acagcttgct ctccacaact atgcctactc agtttctcga
     4441 cactgcggct cgggaactcg gctgtactat atccttgtcc caagcggccg caagtgctgg
     4501 tgaaagcggc gggccggccg acgcccttga cgattttgac ttagacatgc tcccagccga
     4561 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     4621 tgacatgctc cccgggggca agcccatccc caaccccctg ctgggcctgg acagcacctt
     4681 aattaactag cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt
     4741 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg
     4801 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga
     4861 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa
     4921 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc
     4981 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac
     5041 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag
     5101 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc
     5161 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg
     5221 gacgtggttt tcctttgaaa aacacgatga taatatggcc acagccacca tgggcgcgcc
     5281 gcccaagaag aagaggaagg tgaagctact gtcttctatc gaacaagcat gcgatatttg
     5341 ccgacttaaa aagctcaagt gctccaaaga aaaaccgaag tgcgccaagt gtctgaagaa
     5401 caactgggag tgtcgctact ctcccaaaac caaaaggtct ccgctgacta gggcacatct
     5461 gacagaagtg gaatcaaggc tagaaagact ggaacagcta tttctactga tttttcctcg
     5521 agaagacctt gacatgattt tgaaaatgga ttctttacag gatataaaag cattgttaac
     5581 aggattattt gtacaagata atgtgaataa agatgccgtc acagatagat tggcttcagt
     5641 ggagactgat atgcctctaa cattgagaca gcatagaata agtgcgacat catcatcgga
     5701 agagagtagt aacaaaggtc aaagacagtt gactgtatcg ccgggcgacc cactagtcca
     5761 gtgtggtgga attgctgcga gcgatgaagt taatcttatt gagagcagaa cagtggttcc
     5821 tctcaataca tgggttttaa tatccaactt caaagtagcc tacaatatcc ttcgtcgccc
     5881 tgatggaacc tttaaccgac acttagctga gtatctagac cgtaaagtca ctgcaaacgc
     5941 caatccggtt gatggggttt tctcgttcga tgtcttgatt gatcgcagga tcaatcttct
     6001 aagcagagtc tatagaccag cttatgcaga tcaagagcaa cctcctagta ttttagatct
     6061 cgagaagcct gttgatggcg acattgtccc tgttatattg ttcttccatg gaggtagctt
     6121 tgctcattct tctgcaaaca gtgccatcta cgatactctt tgtcgcaggc ttgttggttt
     6181 gtgcaagtgt gttgttgtct ctgtgaatta tcggcgtgca ccagagaatc catacccttg
     6241 tgcttatgat gatggttgga ttgctcttaa ttgggttaac tcgagatctt ggcttaaatc
     6301 caagaaagac tcaaaggtcc atattttctt ggctggtgat agctctggag gtaacatcgc
     6361 gcataatgtg gctttaagag cgggtgaatc gggaatcgat gttttgggga acattctgct
     6421 gaatcctatg tttggtggga atgagagaac ggagtctgag aaaagtttgg atgggaaata
     6481 ctttgtgacg gttagagacc gcgattggta ctggaaagcg tttttacccg agggagaaga
     6541 tagagagcat ccagcgtgta atccgtttag cccgagaggg aaaagcttag aaggagtgag
     6601 tttccccaag agtcttgtgg ttgtcgcggg tttggatttg attagagatt ggcagttggc
     6661 atacgcggaa gggctcaaga aagcgggtca agaggttaag cttatgcatt tagagaaagc
     6721 aactgttggg ttttacctct tgcctaataa caatcatttc cataatgtta tggatgagat
     6781 ttcggcgttt gtaaacgcgg aatgtagcgg cagcgagact cccgggacct cagagtccgc
     6841 cacacccgaa agtgtcactg gttatagact ctttgaagag atactggcgg ccgcagcgag
     6901 agaacatgcg atcggagaag ccaccggaaa acgaaagaaa cgcggcagag ttgaagaagc
     6961 agaagaatac tgtaacgatg gaatcgaaga acaagtagag gatgagaagc ttccgttaga
     7021 ggttgggatg ttctattacc caatgactcc gccttcgttc attgtttccg atgctctgga
     7081 gccagatttt cctttgatct atgtcaacag agtcttcgaa gtcttcactg gctatcgtgc
     7141 cgatgaagtt cttggtcgta actgtcgatt cctacagtac agagatcctc gagctcaaag
     7201 gcgtctccca ttggttgatc ctgtggttgt atctgagatt aggagatgtc ttgaagaagg
     7261 tattgaattc caaggagagc ttcttaattt cagaaaagat ggtactcctt tggttaacag
     7321 actacggctt gctccaatac gtgacgatga tggaaccatt acacacgtaa ttgggataca
     7381 ggtcttctct gaaacggctt acccatacga tgttccagat tacgcttagt ctagagggcc
     7441 ctattctata gtgtcaccta aatgctagag ctcgctgatc agcctcgact gtgccttcta
     7501 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     7561 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     7621 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     7681 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     7741 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7801 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7861 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7921 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7981 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     8041 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     8101 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     8161 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     8221 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     8281 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8341 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     8401 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     8461 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     8521 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     8581 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     8641 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     8701 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     8761 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     8821 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     8881 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     8941 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     9001 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     9061 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     9121 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     9181 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     9241 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     9301 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     9361 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     9421 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     9481 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     9541 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     9601 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9661 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     9721 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     9781 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     9841 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     9901 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     9961 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10021 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10081 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10141 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10201 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10261 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10321 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10381 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10441 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10501 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    10561 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    10621 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    10681 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    10741 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    10801 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    10861 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    10921 cctatctcag cgatctgtct atttcgttca tccatagttg cctgacttcc cgtcgtgtag
    10981 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11041 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11101 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11161 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11221 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11281 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11341 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11401 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11461 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
    11521 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
    11581 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
    11641 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
    11701 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
    11761 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
    11821 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
    11881 ccacctgacg t
//
