LOCUS       sequence-36288-e        1427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Wei JR, Krishnamoorthy V, Murphy K, Kim JH, Schnappinger D, Alber T,
            Sassetti CM, Rhee KY, Rubin EJ
  TITLE     Depletion of antibiotic targets has widely varying effects on 
            growth.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 8;108(10):4176-81. Epub 2011 Feb 
            22.
  PUBMED    21368134
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2011-03-8"; volume: "108(10)"; pages: 
            "4176-81. Epub 2011 Feb 22"
COMMENT     Sequence Label: pJW1
FEATURES             Location/Qualifiers
     source          1..1427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1..24
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      82..438
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     primer_bind     complement(472..488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(472..488)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(590..609)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             601..1410
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(668..687)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gactacaagg acgacgacga caaggccgcc aacgacgaga actactcgga gaactacgcc
       61 gacgcctcgt agctgcagcc atgtcagccg ttaagtgttc ctgtgtcact gaaaattgct
      121 ttgagaggct ctaagggctt ctcagtgcgt tacatccctg gcttgttgtc cacaaccgtt
      181 aaaccttaaa agctttaaaa gccttatata ttcttttttt tcttataaaa cttaaaacct
      241 tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg agagcttagt
      301 acgtgaaaca tgagagctta gtacgttagc catgagagct tagtacgtta gccatgaggg
      361 tttagttcgt taaacatgag agcttagtac gttaaacatg agagcttagt acgtgaaaca
      421 tgagagctta gtacgtacta tcaacaggtt gaactgctgg atccttacat ggtcatagct
      481 gtttcctggc agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa
      541 ataatatcat catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt
      601 atgagccata ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat
      661 ttatatgggt ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc
      721 ttgtatggga agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc
      781 aatgatgtta cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg
      841 accatcaagc attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc
      901 ggaaaaacag cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat
      961 gcgctggcag tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac
     1021 agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat
     1081 gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg
     1141 cataaacttt tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat
     1201 aaccttattt ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc
     1261 gcagaccgat accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca
     1321 ttacagaaac ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag
     1381 tttcatttga tgctcgatga gtttttctaa tcagaattca atctaga
//
