LOCUS       sequence-362880-        9640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline-dependent expression of human cGAS gene in mammalian 
            cells by retroviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9640)
  AUTHORS   Mosallanejad K, Kennedy SN, Bahleda KM, Slavik KM, Zhou W, Govande 
            AA, Hancks DC, Kranzusch PJ, Kagan JC
  TITLE     Species-specific self-DNA detection mechanisms by mammalian cyclic 
            GMP-AMP synthases.
  JOURNAL   Sci Immunol. 2023 Jan 20;8(79):eabp9765. doi: 
            10.1126/sciimmunol.abp9765. Epub 2023 Jan 20.
  PUBMED    36662885
REFERENCE   2  (bases 1 to 9640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Immunol."; date: "2023-01-20"; volume: "8(79):eabp9765. doi"; pages:
            " 10.1126/sciimmunol.abp9765. Epub 2023 Jan 20"
COMMENT     Sequence Label: pTetOne-FLAG-human cGASdeltaN-HA
FEATURES             Location/Qualifiers
     source          1..9640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(13..29)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13..29)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(26..48)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(61..67,68..85,86..91))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(244..261)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(415..1003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(495..514)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1174..1965,1966..2034))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1797..1816
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2035..2139)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2207..2225
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2264..2286)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2386..2405
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2599..2621
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2613..2630
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2614..2630
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2653..2774
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3087..3107)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3790..4193
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4211..4957)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(4976..5486)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5039..5057)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        5503..5870
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    5511..5529
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5547..5565
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5583..5601
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5619..5637
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5655..5673
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5691..5709
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5727..5745
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      5877..5886
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5886..5909
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             6999..7025
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    7209..7343
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7491..7507)
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     primer_bind     complement(7845..7861)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(7877..7899)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     misc_feature    8310..8667
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     enhancer        9098..9477
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
       61 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
      121 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
      181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
      241 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
      301 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      361 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      421 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      481 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      541 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      601 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      661 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      721 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      781 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      841 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      901 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      961 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     1021 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     1081 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     1141 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1201 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1261 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1321 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1381 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1441 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1501 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1561 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1621 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1681 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1741 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1801 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1861 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1921 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1981 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     2041 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     2101 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     2161 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2221 gaggcccttt cgtcatcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc
     2281 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     2341 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     2401 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     2461 accgcatcag gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2521 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     2581 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattaattc
     2641 gacgcgccat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2701 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     2761 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     2821 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     2881 ctctagagtc ggtgggcctc gggggcgggt gcggggtcgg cggggccgcc cgggtcggct
     2941 tcggtcggag ccatggggtc gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg
     3001 gcgtcaggca ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac
     3061 gtcggcggtg acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt
     3121 ctccaggaag gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc
     3181 gctgcccaga cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc
     3241 gggctccttg ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg
     3301 ggaaccgctc aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac
     3361 gctctccggc gtggtccaga ccgccaccgc ggtggcttaa ctatgcggca tacgcgtgaa
     3421 agacccccgc tgacgggtag tcaatcactc agaggagacc ctcccaagga acagcgagac
     3481 cacaagtcgg atgcaactgc aagagggttt attggataca cgggtacccg ggcgactcag
     3541 tcaatcggag gactggcgcc ccgagtgagg ggttgtgggc tcttttgttg atgtctggga
     3601 gcagaagcgc gcgaacagaa gcgagaagct agcttgccaa acctacaggt ggggtctttc
     3661 attcccccct ttttctggag actaaataaa atcttttatt ttatctatgg ctcgtactct
     3721 ataggcttca gctggtgata ttgttgagtc aaaactagag cctggaccac tgatatctcc
     3781 gcaggaatcg acgtcagctt ccccgacaac accacggaat tatcagtgcc cagcaaccta
     3841 gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ctgccgtggc gatcgggagg
     3901 gggaaagcga aagtcccaga aaggagttga caggtggtgg caatgcccca gccagtgggg
     3961 gttgcgtcag caaacacaga gcacaccacg ccacgttgac ggacaacggg ccacaactcc
     4021 tcataaagag acagcaacca ggatttatac aaggaggaga aaacgaaagc cgtacgggaa
     4081 gcaatagcat gatacagagg cattaaagca gcatatccac acagcgtaaa aggagcaaca
     4141 tagttaagaa tatcagtcaa tctttcacaa attttgtaat ccagaggttg attaacagga
     4201 tcttacttag ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca
     4261 gcatatcaag gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt
     4321 caagggcgtc ggtcggcccg ccgctttcgc actttagctg tttctccagg ccacatatga
     4381 ttagttccag gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg
     4441 cttgtttcag aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta
     4501 cttgatgctc ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag
     4561 cgtacagtgc gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca
     4621 gggtttcgta ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat
     4681 gtgagaggag agcacagcgg tatgacttgg cgttgttccg cagaaagtct tgccatgact
     4741 cgccttccag ggggcaggag tgggtatgat gcctgtccag catctcgatt ggcagggcat
     4801 cgagcagggc ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct
     4861 tttgagcgag tttccttgtc gtcaggcctt cgataccgac tccattgagt aattccagag
     4921 cagagtttat gactttgctc ttgtccagtc tagacatggt aattcgatga tccccctggg
     4981 gagagaggtc ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct
     5041 tgcagaatgc ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg
     5101 cctcccgcac cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat
     5161 tggccacagc ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga
     5221 gggtactagt gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg
     5281 aaccttcccg acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg
     5341 cgaagatccg ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc
     5401 cgctgcgttt cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc
     5461 cttggaaaag gcgcaacccc aaccccgtgg aattatcacc tcgagtttac tccctatcag
     5521 tgatagagaa cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt
     5581 actccctatc agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg
     5641 tatgaccagt ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag
     5701 tgatagagaa cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttgc
     5761 ttatgtaaac cagggcgcct ataaaagagt gctgattttt tgagtaaact tcaattccac
     5821 aacacttttg tcttatacca actttccgta ccacttccta ccctcgtaaa gaattcgcca
     5881 ccatggatta caaggatgac gacgataagc ctggggcctc gaagctccgg gcggttttgg
     5941 agaagttgaa gctcagccgc gatgatatct ccacggcggc ggggatggtg aaaggggttg
     6001 tggaccacct gctgctcaga ctgaagtgcg actccgcgtt cagaggcgtc gggctgctga
     6061 acaccgggag ctactatgag cacgtgaaga tttctgcacc taatgaattt gatgtcatgt
     6121 ttaaactgga agtccccaga attcaactag aagaatattc caacactcgt gcatattact
     6181 ttgtgaaatt taaaagaaat ccgaaagaaa atcctctgag tcagttttta gaaggtgaaa
     6241 tattatcagc ttctaagatg ctgtcaaagt ttaggaaaat cattaaggaa gaaattaacg
     6301 acattaaaga tacagatgtc atcatgaaga ggaaaagagg agggagccct gctgtaacac
     6361 ttcttattag tgaaaaaata tctgtggata taaccctggc tttggaatca aaaagtagct
     6421 ggcctgctag cacccaagaa ggcctgcgca ttcaaaactg gctttcagca aaagttagga
     6481 agcaactacg actaaagcca ttttaccttg tacccaagca tgcaaaggaa ggaaatggtt
     6541 tccaagaaga aacatggcgg ctatccttct ctcacatcga aaaggaaatt ttgaacaatc
     6601 atggaaaatc taaaacgtgc tgtgaaaaca aagaagagaa atgttgcagg aaagattgtt
     6661 taaaactaat gaaatacctt ttagaacagc tgaaagaaag gtttaaagac aaaaaacatc
     6721 tggataaatt ctcttcttat catgtgaaaa ctgccttctt tcacgtatgt acccagaacc
     6781 ctcaagacag tcagtgggac cgcaaagacc tgggcctctg ctttgataac tgcgtgacat
     6841 actttcttca gtgcctcagg acagaaaaac ttgagaatta ttttattcct gaattcaatc
     6901 tattctctag caacttaatt gacaaaagaa gtaaggaatt tctgacaaag caaattgaat
     6961 atgaaagaaa caatgagttt ccagtttttg atgaatttta cccatacgat gttccagatt
     7021 acgcttgagg atccagacca cctcccctgc gagctaagct ggacagccaa tgacgggtaa
     7081 gagagtgaca tttttcacta acctaagaca ggagggccgt cagagctact gcctaatcca
     7141 aagacgggta aaagtgataa aaatgtatca ctccaaccta agacaggcgc agcttccgag
     7201 ggatttgaga tccagacatg ataagataca ttgatgagtt tggacaaacc aaaactagaa
     7261 tgcagtgaaa aaaatgcctt atttgtgaaa tttgtgatgc tattgcctta tttgtaacca
     7321 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     7381 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     7441 attatgatct agaggatggt ccacccccgg ggtcggcagc cttcacgtgg gcggcgtgta
     7501 tccaagctgc gatgccgtct actttgaggg cggtgggggt ggtcagcagg actgtgtaag
     7561 gtcctttcca gcgaggttct aggttcttag tctggtgtcg gcggacccac actgtgtcgc
     7621 cgactcggta agggtgaggt accaccggtc ggtccagttg ttcttggtag gctgccgcca
     7681 gaggtctcca gacttcgtgc tggactaagt agagagcctg taagtgagct tggagagagg
     7741 ggctgttagt aactcttgtc atgtcagggt cagggaagtt tacaaggggc gggggtgccc
     7801 catataggat ctcatatggc catatggggg cgcctagaga aggagtgagg gctggataaa
     7861 gggaggatcg aggcggggtc gaacgaggag gttcaagggg gagagacggg gcggatggag
     7921 gaagaggagg cggaggctta gggtgtacaa agggcttgac ccagggaggg gggtcaaaag
     7981 ccaaggcttc ccaggtcacg atgtacccca cctggtctgg gtgtccatgc gggccaggtg
     8041 aaaagacctt gatcttaacc tgggtgatga ggtctcggtt aaaggtgccg tctcgcggcc
     8101 atccgacgtt aaaggttggc cattctgcag agcagaaggt aacccaacgt ctcttcttga
     8161 catctaccga ctggttgtga gcgatccgct cgacatcttt ccagtgacct aaggtcaaac
     8221 ttaagggagt ggtaacaggc tagcccgggc cctcattttc agacaaatac agaaacacag
     8281 tcagacagag acaacacaga acgatgctgc agcagacaag acgcgcggcg cggcttcggt
     8341 cccaaaccga aagcaaaaat tcagacggag gcgggaactg ttttaggttc tcgtctccta
     8401 ccagaaccac atatccctcc tctaaggggg gtgcaccaaa gagtcctaaa cgatcgggat
     8461 tttaggactc aggtcgggcc acaaaaacgg cccccgaagt ccctgggacg tctcccaggg
     8521 ttgcggccgg gtgttccgaa ctcgtcagtt ccaccacggg tccgccagat acagagctag
     8581 ttagctaact agtaccgacg caggcgcata aaatcagtca tagacactag acaatcggac
     8641 agacacagat aagttgctgg ccagcttacc tcccggtggt gggtcggtgg tccctgggca
     8701 ggggtctccc gatcccggac gagcccccaa atgaaagacc cccgctgacg ggtagtcaat
     8761 cactcagagg agaccctccc aaggaacagc gagaccacaa gtcggatgca actgcaagag
     8821 ggtttattgg atacacgggt acccgggcga ctcagtcaat cggaggactg gcgccccgag
     8881 tgaggggttg tgggctcttt tatagagctc ggggagcaga agcgctctag atgcccattt
     8941 gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc ccgttgattt tggtgccaaa
     9001 acaaactccc attgacgtca atggggtgga gacttggaaa tccccgtgag tcaaaccgct
     9061 atccacgccc attgatgtac tgccaaaacc gcatcaccat ggtaatagcg atgactaata
     9121 cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca taatgccagg
     9181 cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga tacacttgat
     9241 gtactgccaa gtgggcagtt tagcgtaaat actccaccca ttgacgtcaa tggaaagtcc
     9301 ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg gggtcgttgg
     9361 gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc atatatgggc
     9421 tatgaactaa tgaccccgta attgattact attaataact agtcaataat caatgtcaac
     9481 atggcggtaa tgttggacat gagccaatat aaatgtacat attatgatat ggatacaacg
     9541 tatgcaatgg gccaagctcc taggcgcata gaacaagtca aatgctttat atatttgagt
     9601 ttgggatgta ttaggaaact cctaagagca aagctagctt
//
