LOCUS       sequence-362922-        9023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmUPF2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9023)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-lambdaN-HA-DmUPF2-V5His6_D
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(972..990)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1058..1162
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1163..1231,1232..2023)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1381..1400)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2194..2782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2683..2702
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    3383..3517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3420..3439)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3474..3493
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3550..3567
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3590..3607)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3617..3658)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             complement(7286..7312)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(7337..7402)
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     promoter        complement(join(7422..9023,1..908))
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
ORIGIN
        1 cgccccgccc cacccttcct tccgctgccc cgcacccctt tccttccact accgttttgc
       61 cgcatttggt attgccatct cgcttttttg gctttacttt gctttttgaa taagccaaga
      121 aactgaaatc aaagcggaca gtccgaaaat tgaatattcc aaaaaacaac aattgcacat
      181 gcactgcgtt cggcaaaaga cgcagaaatt caagcctttt ggtctggcat catcttaaaa
      241 tatcatcagt atcatcattg ccattataaa tacataagaa aatgagaata cattaaaggt
      301 atatacatac aatctttgtc atagtcaaca tattttgcaa caaaattatc tatagataac
      361 atgtttttgt agaaaattcc actaactgat tttcgtataa atacaaatta ttctcattct
      421 tcaaaatact cgccgacgtc atcgcgaaat tcctcagcat tcttcgacaa atgaagagag
      481 acagcgagtg acacaatctt atcttgccga cgagtctttg aaaatggccc cgggtaacgg
      541 tgaaaaacat atagccaaga atggagcgat cgccaggcga tggaaattgg cttcaaattg
      601 ctccgaatat ggccagttgg tcagttggca ttggattgtg gagagatact agatcagctc
      661 gttctccttc tcctccgcat tctcctactc cgcccgaaga cgcttctttg atcgcttctt
      721 tctcgaatgt gtacatattc attatgtatt tggtgtttgg cgacgatcag ccagctttaa
      781 tggccataaa acgtgtcttg gtttgtgggc cacataacag gcagcgagat ctggaaaaat
      841 gatgtgacag tggaaatgag aagtaggtgc atctgcaaag atttgattca gagttgatgc
      901 cattcatgat ttttttttag aagtatcatt tgtgttttta gaatatagaa ttaattcttg
      961 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     1021 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     1081 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     1141 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     1201 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     1261 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     1321 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     1381 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     1441 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     1501 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     1561 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     1621 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1681 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     1741 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1801 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1861 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1921 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1981 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     2041 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     2101 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     2161 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     2221 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     2281 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     2341 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     2401 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     2461 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     2521 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2581 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2641 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2701 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     2761 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     2821 ttgctggcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg
     2881 caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca
     2941 gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat
     3001 aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag
     3061 ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa
     3121 gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat
     3181 aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct
     3241 gactgggttg aaggctctca agggcatcgg tcgactgatc ataatcagcc ataccacatt
     3301 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     3361 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     3421 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3481 gtccaaactc atcaatgtat cttatcatgt ctggatcctc gatcgaggct gatcagcgag
     3541 ctcaggcctt agaaggcaca gtcgaggctg atcagcgggt ttaaactcaa tggtgatggt
     3601 gatgatgacc ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt
     3661 cgaaccgcgg gccctctaga ctcgagcggc cgccggttga ttcgcggcaa tccttggccc
     3721 attgcagaaa ctcatccaac gcgctgacgg ccaaattgcg agaaagagcc ttcacacgca
     3781 gattacgatc ggtggtaatg agcaccaatt ccgtttgaat gaaacacttg ccatctgtgc
     3841 attgttcggt ttttgccgtt ttcgagacag ccattgccgt tgccagaatc ttgtcgtcat
     3901 tggacaaata ttcctcctca actagggcaa acaccgatgc attgacaaac gagccctttg
     3961 tggtagcaca tttcacattg cctttggccg acttgatgaa ctctaacgac tttttggccc
     4021 gcgacgacac ctcgtcaaag tggtggatgc gctgcgtctg cttcgaactg cggtacgagt
     4081 ccaacttaac gcccttggag aggccatcca gttccttaac cactgtcagt ggtataataa
     4141 gtgtgtagcg tttaaactcc gttgacagtt tttcaaagtc ttcaaggcag tcgataaagc
     4201 agttggtatc cggtagaaga tagcgcggtc gcacctcgat gtatatgcgg ccgccactgt
     4261 cccgcagctc ctcctccaaa gtctccacct cgttttgcag ccacagcttg cccatgagct
     4321 cacacttctt cgacttggcc tccgagcgcc cttcgtcggc ccccgagctg gactgcaaat
     4381 tcagtttctc gatgacactg tttgtggttg tggaggcgga ggttccgccg gcgacagagg
     4441 ttcccgtggc actggttata acagcagccg ccttctcggc attgcgattc cttaggtaac
     4501 ccttgcgtgg catcttgcgg cactcctcca cggtgtcctt ttcctcgtta ctggtgtagc
     4561 tcttgcggac gatgccgttc ttgcgatcgg agtagcgacg tgtgcgtcct tcgttccgct
     4621 ttgttcggtt ggtcttcgga gcggtacgac gctcgcggcg acgtgatccg cctcccttct
     4681 tctcctcgcg ctgtttcttc ttttgggtct cggtgaaacg acgcgtcttg acgcagaatc
     4741 tgtagttaaa cttcttctgc ttgcaaatga aaaagccaaa gtcgaccatt ttgaaaatac
     4801 ggagaatgga ctcttcctgg ggagtgacac gctcccggat aatggtctcc cagtccagtg
     4861 taatctgggt gctctggaag atgtcaaagt tcttgaaagc aaagtcctcg gtgagcggaa
     4921 cgttggcgcg cacatcgaat aactcgacca gagaatcacg cacgttggca gtggtcagaa
     4981 ggtcgcgctc gaagtaaacc ttcctggtga agatgtcgat gttgcagtag ttgagaagct
     5041 tcatgatctt gtgcacaaac tccggattcg agtggtagca gccgatgaga atctctgggt
     5101 tgattcgcag ccagtcaaaa agcattttca gggctctaat ggtcggttca ttgtgcagac
     5161 agtcaatgac tatgttggga ttgatcttgg tgtacttgtt gcgataaacc agcttctttg
     5221 gtggctctga aaagctgacg ggcggaggtg ccaccggctt gctttgagca tcctctgcgc
     5281 tcttaaagtt cagctgcagt acctcaaagc tgcgcagcaa ctcctcagag tccgcctcct
     5341 gtgagttgga tctgcgctct acttcgttgg gatacacgat tttctcctcc tcaataacaa
     5401 cgtcttcgtt gtcggagtac accacgccac cgactcctcc tccgccgccg ccgccagctt
     5461 tgcgctcctt tttgctgtaa ttagctgctg catagcgctc ttcgccgcat ggctccccag
     5521 gatcagctgc atcaatgtcg ctgaaatcgg tgtcaaagtt agaacttaga tctgtgtaat
     5581 cctcctcgtc gctgtaaaag tctgtgtcca gttcgctttc gctgtcatag caggagctgt
     5641 cgtccgactg ggcgatcttg cggcgtcggc ggcgtagacg cattccccgt cgcttgctct
     5701 tgcaggagag cggggtctta aggtcggact ccttggcctc gtcactggag cgcgtggaaa
     5761 cggcctcacc gatcttgtcg ccgtcagcag cctcctgggt ctttgcctcc tcgccagagt
     5821 gctggctgtc ccgcgaatcc gaactgcaat cctttagaga gtgcgggcgt ggcatcaact
     5881 tcttgtcctt gcgctcacat tcgcctactg cagctagcca gttcttgtac tcattccggg
     5941 acttgcgcac cacagtatcg aactcctgaa agcgttcctc gtactccgcc tggaagcgct
     6001 cgttgtcttg ggcgcgagcc aggattgtct gctcgatgct gacgatgcag gcgtccatca
     6061 gatcggaaca ggcagcaacc agaaaggcgt gcagcgagta aacctttggt gagttgatca
     6121 tcttaagctt ggacagacag aagaacagaa tggagacaat cttgaagatg cttggctccg
     6181 gaacgcgcag ctgctgggag cacaggatct tgcgtagatc aatcagcaca cagtggcaca
     6241 gagcgttaaa gtccggcact tccttgtcga agaaaaatat gtccacaatc agatagaaac
     6301 gggcaaagaa gtcctcgatg ttgaagtcta gtttttctgg atccatacgc tctaggtatt
     6361 cggtgtgctt ggcgaacagc ttgttaagat tattttcgct tagttcgaat ggtatgatgc
     6421 acacaaggga gtacaagtag tggtaagtgg agtccaggtc atgattctgt ccgtagtgca
     6481 gtgtacccaa ctggttctgg gccatgccaa tggccggatt aagcttgaag gcctccagat
     6541 aatacttggc cacctgctcc ttgcttatgc ttagtttgct gtcgatgcga aagtctagaa
     6601 agtagcgatg aagatcgccc agactgagca gcgatgagtg tatggtgtcg agtgcaaagg
     6661 acacggcctc cagactcttg tccaccgcgt gggtgcccga gtgcgatgac tccatggtgt
     6721 ctccgatcat gtcgcttatg tagccatcac tgattatgga gaaatcaatc aaatacttta
     6781 gatccagatc atagatctct tccatgcgtg ccgacaatcg tttgtagttg aagatgccag
     6841 cgcagaggaa tcgctccagc ttttgcatgt tctcttgggc cgtggccgca tcctggcact
     6901 gcttgtgcca gttcttcttc acgaaggcaa tgaactcgta gtatccacgt cgccacatca
     6961 cctcgcgcac cttcttgccc accgaatggt agtccttgaa gatgatctgc ttgcacagct
     7021 gcactaggag cgagcgtttc tcttcgattt cgtgctggaa cagctgtccc accgactgca
     7081 cattcctctt ggcatcgtcc agttgcttgg aggcggtgta gagggatctg taaagatcct
     7141 ttacctcctg gctgaggatg caggttccat tgttgggctc caagccgcga cagcgattcg
     7201 tgccgccgtc tatggcgcac tcgctggagg cgttcgatga tccgcccgag ctgaatgtca
     7261 cctccatgga attcaagctt ggaggagcgt aatctggaac atcgtatggg taagccatat
     7321 gtggaggaga tccaccgttt gcagctttcc attgagcttg tttctcagcg cgacgctcac
     7381 gtcgtcgtgt ttgtgcgtcc atgttggtac cccgatccgg ggtctctgga ttagacgact
     7441 gctggctgat ggagcggctt tgtgtcggga ggagtatcca cacagcacaa gaactcaaac
     7501 ggtagtgata tgaaaactgg tcccgcagcc cgttttataa agcccgctta gcgctaaaac
     7561 cttatcagcc gcaatgctct ccgctctcaa gtcgcgttca aaacttttac catatatggg
     7621 tatgaattgt tcgccgattg ggttttcatt tacggagaat ttcctccgca actgggtgtt
     7681 cgtgcttgtg tgtgtcttgt atatcgccat gaagagtgta cttccatggg tcctatcagt
     7741 tgcggcatgt gattcaattt gggggttact catattcgat acttcagctg gttatcttta
     7801 tgctgtgatg gtcacggtgc ttttacacat tttttcccca actactcatt gtatgccaaa
     7861 acatacatat gtacatgtgt ttatagtggg tttattagta atgtccagcg atattaaaac
     7921 attacagtta aataatattc atattagctt acgtttactg ctatctgcac ttcaaattgg
     7981 ttattccagg gacattttgt gcaacacaca tcttctagta gggcattcac tagtcgtaga
     8041 aaacacacct aacttaagcc ggttttaaca ataacggcga aacagctgcc agacgagaga
     8101 aaagtacaca gcaagaagca tttctataat gatgccgtcc taaatatgca gcatttttgc
     8161 tcaaattgat catttcgttg catcgctcag gtggtatagt atttggctat tgaatgtgat
     8221 tgctatctta atagttttga atagtaatag tgcaaatatt cattcataaa aaataggtaa
     8281 tcgatttttt cagtgcagtc tatgcgtttg cgcaacagag acgaagagag agacgcctca
     8341 cacacaagca catgcggtct aagctgctct ccctcttttt ctctctctct ctggcttgcg
     8401 gcagttttcc agaggctgtg actcagtaag catttcttgt tgttacccta acgataagcg
     8461 tccgattcct aattctcttt caaataattt gttttatttt tttttttgtg tgttttgaag
     8521 tcaaacagct tcataaacaa tattaaatat cgcaattgtt attgcaaatg cattcgtatc
     8581 aggcgccttg atgtttttcg ccaaaacttc gtctgctctt tgtcagcgta attgcgtttg
     8641 ccggtcgtgc cgtcttgctc gcacgcggct tgcaaacaaa aagtgaaaga gggaaagcca
     8701 cctgtgttgt tgcttttgtt gctatcgttg gctgcacaca catggcaata agtaaccata
     8761 ttgtagactt atttatttta gcgttttcgt tgactaatct ttgtttaaat aattgcctat
     8821 ttcgagccaa aatgacgcat aaaaggaaac ggcaacatgc ggtgcgttga gcatgcgtaa
     8881 gcgagatggc gctagagaga gcgcagcata aaaagaaaaa aacaaccggc gagtggcgag
     8941 acacgaataa tacacacacg cacacatggt caaaggagtg gctccaacgt cggtgacgtc
     9001 gcgcccagcc acagaccccg cca
//
