LOCUS       sequence-362925-        8428 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmBelle.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8428)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 8428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmBelle_B
FEATURES             Location/Qualifiers
     source          1..8428
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2575..2596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2836..2855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3183..3204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5679..5720
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5730..5747
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5770..5787)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5820..5954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5844..5863)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5898..5917
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(6555..7143)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6635..6654)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7314..8105,8106..8174))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7937..7956
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8175..8279)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8347..8365
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaagccaa gcttgatgag taatgctatt aaccaaaatg gcacaggtct agagcagcaa
     3301 gttgctggtc tggacttgaa tggcggctct gctgactaca gcggccccat aaccagcaag
     3361 acttccacta actcggtcac cggtggtgtg tatgtgcccc cgcaccttcg tggtggtggt
     3421 ggcaataaca acgcagcgga cgcagagagc cagggccaag gacagggcca gggtcagggc
     3481 ttcgactcca gatccgggaa tccgcgccag gagactaggg accctcaaca gtcgcgcgga
     3541 ggcggaggcg aataccgcag aggcggcggc ggtggtggtc gcggcttcaa tcgccagagc
     3601 ggcgactacg gttacggaag cggcggcgga ggacgacgtg gaggcggtgg acgtttcgag
     3661 gacaactaca acggtggtga attcgattcg cgtcgcggcg gtgactggaa tcgcagcggc
     3721 ggaggaggtc gaggatttgg acgcggacca tcgtaccgcg gcggtggcgg cggaagcggt
     3781 agcaacctca acgagcagac tgccgaggat ggccaggcac agcagcagca gcagccgcgc
     3841 aacgatcgtt ggcaggagcc agaacgtcct gccggattcg atggcagcga gggcggacag
     3901 tctgccggcg gcaacagaag ctacaacaat cgcggcgagc gcggaggcgg cggttacaac
     3961 agccgctgga aggagggcgg cggctcaaac gtcgactaca caaagctggg cgcccgagat
     4021 gagcgcctgg aggtggagct gttcggcgtg ggcaatacgg gaatcaattt tgacaaatac
     4081 gaggatatac ccgtggaggc gacgggccag aatgtgcccc cgaacatcac atcgttcgat
     4141 gatgtgcagc tgacggagat tatccgcaac aacgtggcct tagcacgtta tgacaaaccg
     4201 acaccggtgc agaagcacgc cattccgatc attatcaatg gccgggatct aatggcctgc
     4261 gcccagactg gatccggcaa gacggccgcc ttcctggtgc cgattctcaa ccagatgtac
     4321 gagctgggac atgtgccccc gccgcaaagc acccggcagt acagccggcg caagcagtat
     4381 ccgttgggtc tggtgctggc gccgacccgc gaactggcca cccagatctt tgaggaggcc
     4441 aagaagttcg cctatcgctc ccggatgcgc ccggcagtgc tgtacggcgg aaacaatact
     4501 agcgagcaga tgcgcgagct ggaccgcggt tgccatctga ttgtggccac acccggtcgc
     4561 ctagaggaca tgatcacacg cggaaaggtg ggactggaga acattcgatt ccttgtactg
     4621 gatgaggctg atcgtatgtt ggacatgggt ttcgagccgc agatccgtcg catcgtcgaa
     4681 cagctaaaca tgccgccaac cggacagcgc cagacgctta tgttctcggc cacattcccc
     4741 aagcagatcc aggagttagc cagcgatttc ctcagcaact acattttctt ggccgttggt
     4801 cgtgtgggct ccacatctga gaacattacg cagacaatcc tgtgggtcta tgaacccgac
     4861 aagcgctcgt atctgctgga tctgctctcg tctatccgcg atggccccga atacaccaaa
     4921 gacagcctaa cactgatctt tgtggagact aagaagggtg cagactcgct ggaggagttc
     4981 ctgtaccagt gcaatcatcc cgtcactagc atccacggtg atcgcacgca gaaggagcgc
     5041 gaggaggcac tgcgctgctt ccgatcgggc gactgcccca tcctggtggc cacagccgtg
     5101 gccgctcgtg gcctggacat tccgcacgtt aagcacgtta ttaactttga cctgccctcg
     5161 gatgtggagg agtatgtcca ccgtatcggg cgtaccggac gtatgggtaa tcttggtgtg
     5221 gccacctcct ttttcaacga aaagaaccgc aacatttgct ccgatctctt ggagctgctg
     5281 atcgaaacga agcaggagat ccccagtttc atggaggata tgagttcgga tcgcggacat
     5341 ggcggagcca agcgggctgg acgaggcggt ggtggacgtt atggtggcgg cttcggctcc
     5401 agggattacc gtcaaagctc tggcggcggc ggtggcggac gcagtggacc gccaccacgt
     5461 agcggtggtt ccggatcagg aggcggcggc ggcagttacc gcagcaatgg aaactcgtac
     5521 ggcggcaatt cgggcggcgg tggatactat ggcggcggcg ccggtggtgg ctcctatggc
     5581 ggctcctacg gcggcggcag tgcctcgcac tcttcgaacg cacccgactg gtgggctcaa
     5641 tgagcggccg ctcgagtcta gagggcccgc ggttcgaagg taagcctatc cctaaccctc
     5701 tcctcggtct cgattctacg cgtaccggtc atcatcacca tcaccattga gtttaaaccc
     5761 gctgatcagc ctcgactgtg ccttctaagg cctgagctcg ctgatcagcc tcgatcgagg
     5821 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     5881 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     5941 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     6001 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc
     6061 agtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
     6121 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
     6181 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     6241 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt
     6301 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg
     6361 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt
     6421 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta
     6481 gatgacgacc atcagggaca gcttcaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6541 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6601 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6661 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6721 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6781 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6841 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6901 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6961 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7021 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7081 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7141 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7201 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7261 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7321 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7381 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7441 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7501 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7561 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7621 gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7681 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7741 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7801 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7861 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7921 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7981 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8041 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8101 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8161 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8221 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8281 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     8341 tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattaattct atattctaaa
     8401 aacacaaatg atacttctaa aaaaaaat
//
