LOCUS       sequence-362935-        1033 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vitro transcription of heiCas9 mRNA - mammalian codon 
            optimized Cas9, N-terminal hei-tag (Myc-flexible 
            linker-optimizedNLS) and C-terminal oNLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Thumberger T, Tavhelidse-Suck T, Gutierrez-Triana JA, Cornean A, 
            Medert R, Welz B, Freichel M, Wittbrodt J
  TITLE     Boosting targeted genome editing using the hei-tag.
  JOURNAL   Elife. 2022 Mar 25;11. pii: 70558. doi: 10.7554/eLife.70558.
  PUBMED    35333175
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-03-25"; volume: "11. pii: 70558. doi"; pages: " 
            10.7554/eLife.70558"
COMMENT     Sequence Label: pCS2+ heiCas9
FEATURES             Location/Qualifiers
     source          1..1033
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..39
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 tttaccatgg agcagaagct gatcagcgag gaggacctgg gaggaagcgg accacctccc
       61 aagaggccca ggctggacct cgaggataaa aagtattcta ttggtttaga catcggcacc
      121 aacagcgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
      181 gtgctgggca acaccgacag acacagcatc aagaaaaacc tgatcggcgc cctgctcttc
      241 gactccggcg aaaccgccga ggccaccaga ctgaagagaa ccgccagacg gagatacacc
      301 agacggaaga atagaatctg ctacctgcag gagatcttca gcaacgagat ggccaaggtg
      361 gacgatagct tctttcacag actggaagag agcttcctgg tggaagagga caagaaacac
      421 gagagacacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
      481 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgagactg
      541 atctacctgg cactggccca catgatcaag ttcagaggcc acttcctgat cgagggcgac
      601 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
      661 cagctgttcg aagagaaccc tatcaacgcc agcggcgtgg acgccaaggc catcctgagc
      721 gccagactca gcaagagcag acggctggag aacctgatcg cccagctgcc cggcgagaag
      781 aaaaacggcc tgttcggcaa cctgatcgcc ctgagcctgg gcctgacccc caacttcaag
      841 agcaacttcg acctggccga ggacgccaag ctgcagctga gcaaggacac ctacgacgat
      901 gacctggaca acctcctggc ccagatcggc gaccagtacg ccgacctgtt cctcgcagcc
      961 aagaacctga gcgacgccat cctcctgagc gacatcctca gagtgaacac cgagatcacc
     1021 aaggctcccc tga
//
