LOCUS       sequence-362938-        9169 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmStaufen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Behm-Ansmant I, Rehwinkel J, Doerks T, Stark A, Bork P, Izaurralde E
  TITLE     mRNA degradation by miRNAs and GW182 requires both CCR4:NOT 
            deadenylase and DCP1:DCP2 decapping complexes.
  JOURNAL   Genes Dev. 2006 Jul 15. 20(14):1885-98.
  PUBMED    16815998
REFERENCE   2  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2006 Jul 15. 20(14):1885-98."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmStaufen-V5His6_C
FEATURES             Location/Qualifiers
     source          1..9169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2585..2587,2588..2590,2591..3301)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2585..2587,2588..2590,2591..3301)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2630..2651)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2891..2910)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3238..3259
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3332..3358
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     RBS             5944..5952
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             6475..6516
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6526..6543
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6566..6583)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6616..6750
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6640..6659)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6694..6713
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7351..7939)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7431..7450)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8110..8901,8902..8970))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8733..8752
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8971..9075)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9143..9161
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 catcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2641 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2701 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2761 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2821 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2881 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2941 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3001 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3061 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3121 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3181 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3241 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3301 gccaagcttg aattctgcag atatcatggc ttacccatac gatgttccag attacgctat
     3361 gcagcacaac gttcatgccg cccggccagc gccccacatc cgagctgcgc accatcacag
     3421 ccacagccat gcccacatgc atctgcatcc cggcatggag cagcacctgg gccccagtct
     3481 tcagcagcaa cagcagccgc caccgccgcc ccagcagcct ccacatcgcg acctccacgc
     3541 acgtctgaat catcaccatc tccacgctca gcagcagcag cagcaacaga cgtcgtcgaa
     3601 ccaagccgcc gccgtcgctg cggctggggc tgcctatcac cacggcaaca tcaacagcaa
     3661 cagcggcagc aacatctcca gcaacagcaa ccagatgcag aagatccgcc aacagcacca
     3721 gcacttgagt agcagcaacg gccttcttgg caatcaacct cctggtccgc cgccacaggc
     3781 cttcaatcca ttggcgggca atccggcagc tctcgcctac aatcagcttc ctccgcatcc
     3841 gccgcaccac atggccgccc atctgggcag ctatgccgct ccaccgcctc actattacat
     3901 gagccaggcg aagcccgcga aatataatca ctatggcagc aatgcaaaca gcaacagcgg
     3961 cagcaacaac agcaacagca actatgcccc caaggccata ctgcagaata cgtataggaa
     4021 tcagaaggtg gtggttccac cagtggtgca ggaagtaacc ccggtgccgg aaccgccagt
     4081 gaccaccaat aatgccacca ccaacagcac cagcaacagc acagtcatag ctagcgagcc
     4141 tgtaacccaa gaggatactt ctcagaagcc ggagactaga caggagccag cttcagcgga
     4201 cgatcatgtc agcacgggga atatcgatgc tacgggagca ctgtccaacg aggacacgag
     4261 cagttcagga cgcgggggca aggacaagac cccaatgtgc ctggtcaatg agttggccag
     4321 gtacaacaag atcacgcatc aatatcggtt gactgaagaa cgaggacctg cccactgcaa
     4381 gaccttcacg gtgacactga tgctgggcga tgaggagtac tcggcggatg gttttaagat
     4441 taagaaagcg caacatttgg cggctagtaa ggctatcgag gaaaccatgt acaagcatcc
     4501 gccgccgaag attcgccgca gcgaagaagg cggtcccatg cgcacacaca ttacgccgac
     4561 ggtggaattg aatgccctgg ccatgaagtt gggacagcgc accttctacc tgctggatcc
     4621 cactcagatt cctcccacgg attccatagt gccgccggag tttgccggcg gtcatttact
     4681 aactgcacca ggaccaggga tgccgcaacc acctccacca cctgcttacg ctctgcgcca
     4741 gcggcttggc aatggatttg tgcccattcc atcgcagccg atgcatccgc atttctttca
     4801 tggacctggt cagcgaccgt ttcctccgaa gtttccatca cgctttgcgc tgccaccgcc
     4861 tctcggagct catgtacatc atggaccaaa tggcccattt ccatccgtgc ctacgccacc
     4921 cagcaaaatc accctgtttg tgggcaagca gaagtttgtg ggcatcggac gcacactgca
     4981 gcaagcgaaa catgacgctg cggcgagagc gctgcaggtt ctcaagacac aggccatatc
     5041 tgcctctgag gaggctctcg aggattcgat ggatgagggt gacaagaagt cgcccatctc
     5101 gcaggttcac gagatcggaa ttaagcgcaa catgaccgtt cattttaagg tgttgcgcga
     5161 ggaggggccg gcgcacatga agaactttat aacagcctgc attgtgggat ctattgtcac
     5221 cgaaggagaa ggaaatggca aaaaagtgtc caagaagcgt gctgcggaaa agatgctcgt
     5281 cgagttgcaa aagctgccgc cccttacgcc caccaagcag acgccattga agcggattaa
     5341 ggttaagaca ccaggcaaaa gtggtgcagc tgcccgagaa ggttcggttg tgtcgggcac
     5401 tgatggtact atgcaaacgg gaaagccgga gagacgtaaa cgcctcaatc cgccgaagga
     5461 caagctgata gacatggacg atgcggataa tcccattaca aagctaattc aactgcagca
     5521 gacgcgtaag gaaaaggaac ccattttcga gctgatagcc aaaaacggca atgaaaccgc
     5581 tcgacgacgg gaattcgtta tggaggtctc cgccagcggg agcacagccc gtggaacagg
     5641 caacagcaag aaattggcaa agcgaaatgc tgcacaggct ctatttgaac tgctggaagc
     5701 tgttcaagtc acaccaacca acgaaacaca gtcatcggag gaatgctgca cttcggcaac
     5761 aatgtccgcc gttacagcgc ccgcagtgga ggcaactgcg gaaggcaaag tgcccatggt
     5821 agcaacgcca gtgggtccca tgccaggcat tttaatactg cgccagaaca agaaaccagc
     5881 aaagaagagg gatcaaattg taattgtcaa gtctaacgtg gaatccaaag aggaggaggc
     5941 caataaggag gtggcagttg cagctgagga gaatagcaac aacagtgcaa actctggcga
     6001 cagcagcaac agcagcagcg gcgattcgca agccacagag gcggctagtg agagtgcact
     6061 gaatacctca accggcagca atacgagtgg tgtgagtagc aacagcagca atgttggagc
     6121 caacacggat ggtaacaacc atgccgagag taaaaacaac acggagagta gcagcaacag
     6181 tacgagtaac acacaaagcg ctggagtgca catgaaggag cagctcttgt accttagtaa
     6241 actgctggac tttgaggtca acttctcgga ctatccgaaa ggcaatcaca acgagttcct
     6301 gaccatcgtg acattgtcca cacatccgcc gcagatttgc catggcgttg gcaagagctc
     6361 cgaggagtcg cagaatgatg ctgcaagcaa tgccttgaaa atcctcagca agctggggct
     6421 caacaatgcc atgaaataag cggccgctcg agtctagagg gcccgcggtt cgaaggtaag
     6481 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
     6541 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctaaggcctg agctcgctga
     6601 tcagcctcga tcgaggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     6661 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     6721 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     6781 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     6841 atggctgatt atgatcagtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     6901 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     6961 actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg
     7021 gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca
     7081 agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg
     7141 catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc
     7201 cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
     7261 gctgtccagg caggtagatg acgaccatca gggacagctt caaggccagc aaaaggccag
     7321 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     7381 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     7441 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     7501 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     7561 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     7621 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     7681 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     7741 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     7801 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7861 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7921 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7981 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8041 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8101 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8161 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8221 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8281 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     8341 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     8401 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     8461 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     8521 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     8581 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     8641 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     8701 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     8761 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8821 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8881 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8941 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9001 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9061 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9121 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgt
//
