LOCUS       sequence-362941-        9038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmSmaug.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Eulalio A, Behm-Ansmant I, Schweizer D, Izaurralde E
  TITLE     P-body formation is a consequence, not the cause, of RNA-mediated 
            gene silencing.
  JOURNAL   Mol Cell Biol. 2007 Jun . 27(11):3970-81.
  PUBMED    17403906
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Jun . 27(11):3970-81."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmSmaug-V5His6_C
FEATURES             Location/Qualifiers
     source          1..9038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2585..2587,2588..2590,2591..3301)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2585..2587,2588..2590,2591..3301)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2630..2651)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2891..2910)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3238..3259
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6344..6385
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6395..6412
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6435..6452)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6485..6619
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6509..6528)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6563..6582
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7220..7808)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7300..7319)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7979..8770,8771..8839))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8602..8621
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8840..8944)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9012..9030
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 catcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2641 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2701 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2761 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2821 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2881 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2941 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3001 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3061 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3121 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3181 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3241 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3301 gccaagcttg aattctgcag atatcatgaa gtacgcaact ggaactgaca acgcgatgac
     3361 ctccggcatc agcggccaaa ccaataactc taacagtgca tccaacgaga tgcagccaac
     3421 cacatcaacg cctactgcag cccacaagga ggccacatca acggccacga cgacggcgac
     3481 atacgccaat ggcaatccca attctaacgc caatcccagc cagagccagc cgtccaatgc
     3541 gctgttttgc gagcaggtga ccacagtgac aaatctcttc gagaagtgga acgactgcga
     3601 gcgaaccgtc gtgatgtacg cgctgctcaa gcggctgcgc tacccgagcc tcaagtttct
     3661 acagtattct atcgacagca acctgaccca gaacctgggc acctcgcaga ccaatctgag
     3721 cagcgtggtt attgacatca atgccaacaa tccggtgtac ctgcagaatc tactcaacgc
     3781 ctacaagacg ttccagccat gtgacctgct cgacgccatg tcctcctcgt cgtccgacaa
     3841 ggactcgatg ccatgttacg gcagtgactt ccagatcacc acatcagcgc agtgtgacga
     3901 gcgaaagctg tatgcccgca aggaggacat tctgcatgaa gtactgaaca tgctgcctct
     3961 gctgaagccg ggcaatgagg aggccaagct tatctacctg accctcatac cagttgccgt
     4021 caaggacacc atgcagcaaa ttgtgcccac ggagttggtg cagcagatct tctcgtacct
     4081 actcatccat ccagctatca ccagcgagga cagacgttcg ctcaacattt ggctgcgtca
     4141 cttggaggat catatccaag cggctgcggc gggcctgaca aatcgcagtt acttcctgca
     4201 gccctcgccg caactggtcg ctggtggtag ctcaacaggc agtggtagct gttcctcttc
     4261 ggcgaccagc tcttcgacag catcctgttc gtcggtggcc tcatcctcgt tgtgccccgc
     4321 aagcggcagc cgttcctcac gcaccaacga ctggcaaacg atcgccccgc ccagcaagca
     4381 attgcaaaac aaattggccg gtgattggcg tggcaatgga ggcggcagct ccagcgggtc
     4441 gatcaatcca ctctgtgata atcttaatgg tattaccctg aacgaattag cttctagtca
     4501 gaacagtctc ggcctgtcgc tggagggcag ctcttccctg gtcaacggtg tggtcgctgg
     4561 tgcaggctct atgttgggaa tcgctggtgg cgacgatcat gacacgtcat ttagcaagaa
     4621 tggcacggag attctcgatt ttgacccggt gacggcggac atgggtgagg cctgtagtct
     4681 ggccagcagt agcctttgtg gacgtaacgg tggaaatcct gttgaggatc gatcgcaacc
     4741 tccgccgaat cttcagcagc aattgcttca gcctcctccc tatgcctcga tccttatggg
     4801 aaacgtgggc gatcagtttg gagagatcaa ccggtggagc ctggacagca agattgcagc
     4861 cctgaagacg cggcgctcta acagcctcac cacgcaaacg atctctagct gttccagctc
     4921 gtctaactca tcggtgatca cggtcaatga caactgctcc aattcaacgg agaatctggc
     4981 acagtttgcc aacaagcccc gtagtttttc cttatcgatt gagcatcagc gcggcgcctt
     5041 gatgaacagc ggttccgata ctcgtctgga tgaattcaag cccaattata ttaagttcca
     5101 cacgcgcaac gtgggcatgt cgggaatcgg actctggcta aaatccttgc gtctgcacaa
     5161 gtacatcgag ctgttcaaga acatgacgta cgaggaaatg ctcttgatca ccgaggactt
     5221 tctgcagagt gttggcgtca ccaagggcgc ctcccacaag cttgcccttt gcattgataa
     5281 gctgaaggaa cgggccaata ttcttaaccg ggtagaacaa gaactgctat cgggtcaaat
     5341 ggagctgagc acggcggttg aggagctaac aaatattgtg ctgacaccaa tgaagccact
     5401 ggagtctcca ggaccaccgg aggaaaacat tggtctgcgt tttcttaagg tcattgacat
     5461 tgtgaccaat acgttgcagc aggatccgta tgctgtccaa gacgacgaga ctttgggtgt
     5521 gcttatgtgg attctagacc gttctatcca caacgaggca tttatgaatc atgccagtca
     5581 actcaaggac ctgaagttta agttgtccaa gatgaagatc tccatggttc ccaaaatgca
     5641 tcacgttaag ccagccggcg tcgggccaaa caatgggaac attaacaagc caagatggaa
     5701 tggtaagact cgcaaatgcg atactaaaaa tggcagcaac gataggatta acaaccgcaa
     5761 aaactccaac gacatgctaa acttttcgct aaattgcctg ccacacccgc tgccccatca
     5821 ttcgcagcag gctccgccgc ctctgccaca gtttgactac aacggctatg gcggcggtcc
     5881 atcccatcag ccgcagtaca agagctcctc gtacccaagc ttcatgggta atcctcagca
     5941 gcagccacca ccgccgccgt ctagtaagtc gcatcatcat ccccagcaga tgcaacagat
     6001 gctgcagcaa cacaaccact ttccagcgct gcctcagcag acgccaccac aatcgcatcg
     6061 ccggtcgctg aataatctta ttctggtggc cggaggtccg cagcaaccgc agcaattgat
     6121 ctttaagccc ggtcagggag tgctcaccaa caacggatct aacgacaacc tggtattgga
     6181 acgcaatcag cagtcgcagc agcagagaaa acttagcgga ggagtgtcca gtgcggagca
     6241 acaaccaaag aagaccatgg ccgctgttgt gatggaaaat ttggccaaat ttgatcaaca
     6301 ttttacgcta ttccgctcga gtctagaggg cccgcggttc gaaggtaagc ctatccctaa
     6361 ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc attgagttta
     6421 aacccgctga tcagcctcga ctgtgccttc taaggcctga gctcgctgat cagcctcgat
     6481 cgaggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6541 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6601 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6661 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
     6721 tgatcagtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     6781 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     6841 aggtgccggc agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga
     6901 tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca
     6961 ctggtcccgc caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg
     7021 acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta
     7081 tgattcttct cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc
     7141 aggtagatga cgaccatcag ggacagcttc aaggccagca aaaggccagg aaccgtaaaa
     7201 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     7261 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     7321 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     7381 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     7441 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     7501 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     7561 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     7621 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7681 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7741 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7801 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7861 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7921 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7981 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     8041 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     8101 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     8161 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     8221 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     8281 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     8341 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     8401 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     8461 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     8521 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     8581 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca
     8641 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8701 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8761 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8821 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8881 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8941 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     9001 taacctataa aaataggcgt atcacgaggc cctttcgt
//
