LOCUS       sequence-362945-       10124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmGe1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10124)
  AUTHORS   Eulalio A, Behm-Ansmant I, Schweizer D, Izaurralde E
  TITLE     P-body formation is a consequence, not the cause, of RNA-mediated 
            gene silencing.
  JOURNAL   Mol Cell Biol. 2007 Jun . 27(11):3970-81.
  PUBMED    17403906
REFERENCE   2  (bases 1 to 10124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2007 Jun . 27(11):3970-81."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmGe1-V5His6_A
FEATURES             Location/Qualifiers
     source          1..10124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..2510
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2530..2532,2533..2535,2536..3246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2575..2596)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2836..2855)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3183..3204
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      5102..5111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7375..7416
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7426..7443
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(7466..7483)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7516..7650
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7540..7559)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7594..7613
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(8251..8839)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8331..8350)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9010..9801,9802..9870))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9633..9652
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9871..9975)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10043..10061
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 catgaatggc atcaactctg aatcaaatct ttgcagatgc acctacttct catttccact
       61 gtcacatcat ttttccagat ctcgctgcct gttatgtggc ccacaaacca agacacgttt
      121 tatggccatt aaagctggct gatcgtcgcc aaacaccaaa tacataatga atatgtacac
      181 attcgagaaa gaagcgatca aagaagcgtc ttcgggcgga gtaggagaat gcggaggaga
      241 aggagaacga gctgatctag tatctctcca caatccaatg ccaactgacc aactggccat
      301 attcggagca atttgaagcc aatttccatc gcctggcgat cgctccattc ttggctatat
      361 gtttttcacc gttacccggg gccattttca aagactcgtc ggcaagataa gattgtgtca
      421 ctcgctgtct ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag
      481 tattttgaag aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac
      541 aaaaacatgt tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt
      601 atgtatatac ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac
      661 tgatgatatt ttaagatgat gccagaccaa aaggcttgaa tttctgcgtc ttttgccgaa
      721 cgcagtgcat gtgcaattgt tgttttttgg aatattcaat tttcggactg tccgctttga
      781 tttcagtttc ttggcttatt caaaaagcaa agtaaagcca aaaaagcgag atggcaatac
      841 caaatgcggc aaaacggtag tggaaggaaa ggggtgcggg gcagcggaag gaagggtggg
      901 gcggggcgtg gcggggtctg tggctgggcg cgacgtcacc gacgttggag ccactccttt
      961 gaccatgtgt gcgtgtgtgt attattcgtg tctcgccact cgccggttgt ttttttcttt
     1021 ttatgctgcg ctctctctag cgccatctcg cttacgcatg ctcaacgcac cgcatgttgc
     1081 cgtttccttt tatgcgtcat tttggctcga aataggcaat tatttaaaca aagattagtc
     1141 aacgaaaacg ctaaaataaa taagtctaca atatggttac ttattgccat gtgtgtgcag
     1201 ccaacgatag caacaaaagc aacaacacag gtggctttcc ctctttcact ttttgtttgc
     1261 aagccgcgtg cgagcaagac ggcacgaccg gcaaacgcaa ttacgctgac aaagagcaga
     1321 cgaagttttg gcgaaaaaca tcaaggcgcc tgatacgaat gcatttgcaa taacaattgc
     1381 gatatttaat attgtttatg aagctgtttg acttcaaaac acacaaaaaa aaaaataaaa
     1441 caaattattt gaaagagaat taggaatcgg acgcttatcg ttagggtaac aacaagaaat
     1501 gcttactgag tcacagcctc tggaaaactg ccgcaagcca gagagagaga gaaaaagagg
     1561 gagagcagct tagaccgcat gtgcttgtgt gtgaggcgtc tctctcttcg tctctgttgc
     1621 gcaaacgcat agactgcact gaaaaaatcg attacctatt ttttatgaat gaatatttgc
     1681 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
     1741 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
     1801 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
     1861 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgaatgcc ctactagaag
     1921 atgtgtgttg cacaaaatgt ccctggaata accaatttga agtgcagata gcagtaaacg
     1981 taagctaata tgaatattat ttaactgtaa tgttttaata tcgctggaca ttactaataa
     2041 acccactata aacacatgta catatgtatg ttttggcata caatgagtag ttggggaaaa
     2101 aatgtgtaaa agcaccgtga ccatcacagc ataaagataa ccagctgaag tatcgaatat
     2161 gagtaacccc caaattgaat cacatgccgc aactgatagg acccatggaa gtacactctt
     2221 catggcgata tacaagacac acacaagcac gaacacccag ttgcggagga aattctccgt
     2281 aaatgaaaac ccaatcggcg aacaattcat acccatatat ggtaaaagtt ttgaacgcga
     2341 cttgagagcg gagagcattg cggctgataa ggttttagcg ctaagcgggc tttataaaac
     2401 gggctgcggg accagttttc atatcactac cgtttgagtt cttgtgctgt gtggatactc
     2461 ctcccgacac aaagccgctc catcagccag cagtcgtcta atccagagac cccggatcgg
     2521 ggtaccatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2581 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2641 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2701 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     2761 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2821 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2881 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2941 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3001 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3061 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3121 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3181 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3241 tacaagccaa gcttgaattc tgcagatatc cagcacagtg gcggccgcat gttaatcgcg
     3301 ctcttcgcgc ttgcacactt gccaatcttt cgtaagacgc ctttgtcagc ctcctcctcc
     3361 ccgccgccgt ctgtccaccg gtcgccaagg tgtggcaaag caagtaccgc gtaccatcca
     3421 ccgccagcag acgcctgcat cagcattact gcttcagcca ttgacgcagc atccgggatg
     3481 agcaacagcg ccgaacagga caagccgatt gccgccaacg gcaatgcggc agagccccag
     3541 gaaatagaag tcatccattt ccaagcgcaa gagaagcagt gttgccgcgt tattagatcc
     3601 aataagacca aggtcctcac atccggcggc gttcacacac gcggttcctc caaggtcaag
     3661 ctaaagaaca tcgtcgacta caagtgggag cgcaagtact actatccggg tcacctggtc
     3721 gctgtccacc gcgatggcaa gcacctggcg tacgccatta atgtcaacaa caaagccacc
     3781 ggaatggagg gcatggtgcg cgtatgcaac atagccacca gtatgcgagc cttgatcaag
     3841 ggaatgagcg gcgaggtctt agatctgcag tttgcccata cggattgtga gcgtatatta
     3901 gctgtgatcg atgtcagttc gctgtttgtc tacaaagtcg atcaaatcga agggaacctg
     3961 ctgtgcaatc tagtgctaaa ggtggaggac cccatcgcca actatgtgcc ggagtacgat
     4021 atggtctcct ggtgtcccta tgtctgcagc agcagcgcca ccgttccaat caacgacgat
     4081 gacgatgaga atcagctgct catttggtcg cgaagctctc aatttcaatg ctttcaagtg
     4141 aagatgattg tctctgaaca tgggcgcggc aagatccaac cagcagccct agaatctgga
     4201 tatctgaaga ttgaggagga ctcgttgatc acgtgcgccg ctttgtcccc ggatggcacc
     4261 actgtggctg ctgcttgcgc cgatggcttg gttcgtttct accaaattta tttgttcgat
     4321 gtgcgcaacc atcgctgcct gcacgagtgg aagccacatg atggcaaaaa agtctgctct
     4381 ctcttttttc tcgacaatat caacaagcct gtggaagaat cctactggca gcacgtgatc
     4441 actacgagcg atgccaatac cgaaatcaag ctctggaact gctcgctatg gaaatgcctc
     4501 caaactatca atgtggtggc cagtccgtca tctttgcagc ccagaaactt tatcgcgggc
     4561 atagatcgca gtgcgaacta cttggtgttg tcttgcctgg actctctggc cgtgtatgta
     4621 atgcagattg gcagtactgg cggcgcagac tccgagaata ggagtagcga tagcgaagga
     4681 gaaggctgcg acacttccaa acgcattcag aatgttgccg aattcaagct gagttcgggg
     4741 attctctctt tttctattgt aaacgctagt atgcgtcgcg ttaagaactc catcgagagc
     4801 tactatccta ttgaggagcc cgatgacttc gatgacgatt ccaattccac cagtgccctt
     4861 gtgctgcaca tgtttgtggt gcaggcaaag agcctgcagg aatgtcaaat aatctaccag
     4921 ccatgtgtgg ctgaaaagac ggagcggagc agcctaaaca gcaagcgatc acagacgcca
     4981 gaagacaatc tgctaatcaa agaggagccg gaatccccca acagtggaac tgtgggtgct
     5041 gttcagctgg atgcattatt cgcaaaatcc gcaaagagag cttcaacggg cagttcatct
     5101 gccatggtgg ccgtggcggc agccgcggct gcggcacctt cggccatcct gcaggacgcc
     5161 actaaggagg cggccaagtc agagtccccg caactaagca gcgcgtatac gcagcaggtt
     5221 aatctcatga ctccagatgc cttcagtgca agcggaaccg cagcggcagc agctgtattt
     5281 gtgtctacca gcaccacaac ttcaatagga actgatagct ctaccaccac cagcggtcag
     5341 gatagatcca ttgattcagc ggttctgcaa actatacgaa tgctggccac ggtaacgtcc
     5401 aagacttccg aaaaccccaa tgccgaggta ctcctcaacc taatgaacaa caccctaatc
     5461 gaagatcgcg agcaacaaaa gctgaaggaa aagcttgacg ctcgcaagaa gttcattgca
     5521 attgatcgca atccagagcg gaatgtggct gagaacttgg ccagcggcag ttcgagtccg
     5581 agtcgagagg tgcaggagat aatggccaca caggacgatg ccgatgccta cgaagcagag
     5641 ctagagaatc tggacgacga tgatgacgat gaggaagaag agctggcaaa ttcatcaccg
     5701 ctcccggaag ctgttgacgg aacatggccc attgtcaagc tgtcatccca ctcagctgcg
     5761 ctccagaatg ctgcccagat catgtcccag gcggtgcaga acaccaacaa tggcaatgtg
     5821 ccgcctacgc taggtggagg gcacaataat aacacaagtg taggcagcaa tagcaacaac
     5881 aacacggcta caactcttag caccagcaac accagcagca gcaacaacgc gggcggaaca
     5941 tgcgttgatt caagtggaac tggagagcta aatgccaaaa tggaactact catagatcta
     6001 gtaaaagccc agtccaaaca aataaacaaa ttggagaacg aagtcaacaa gctacaaaag
     6061 cagcaagaag cggcggcggc cctgcatagc aaacaagaca cttcattgga gcctaaaaat
     6121 cttagtcagc tagcatataa aattgagatg caactgtcca agctaatgga gcagtatctc
     6181 aagcgatatg aaaacgagca taagaagaaa ctgacggaat tcttggcggc gcgtgagagc
     6241 caaaaccgag agctacgtga ctccgtgctg caggtactaa atcagtatgt catgaaccac
     6301 tttacggaca ttattggcaa cgttctcaac atggaactgc agcgccaact gcttcctcgc
     6361 gtgaatgcca acatggatca gctccaagcc caaatgcaag tggagattgt gcagaagctg
     6421 agtgtgtttg acaaaacggt gaaggagaat attgcccagg tgtgcaagag caagcaattt
     6481 ctggatactt ttggcaagtc cgtgttgatt ggtgctcaaa ccagcctaca aaccgccttc
     6541 attgaatcta tgagcagcac cttgattcca gcttatgaga agtcctcgca gaacatgttt
     6601 aaacaactgc atgacgcttt cagcgtgggc ataaaagact ttatggtcca gtttaacacc
     6661 tacctgcagc acatgccaca gccccaagcc gggagtggca atacggagga gataaacaac
     6721 aagttgtcca tgcttaagca attggtagag agcagcttgc acaagcaccg cactgaactc
     6781 acggacgcca tgctcgagac acaaagagaa gtgaagagct tagagatttt gctggcgcgt
     6841 caggtacagg aaacgatcag agcagagcta cgcaagcaca tggaagccca gaacgtggca
     6901 atgcgttcgc aggcagctac gcctgcacca ccttacgacc tcagggacag cattaagcag
     6961 cttttgatgg ctggacaaat caacaaggcc ttccatcagg ctctattggc caacgacttg
     7021 ggcctagtgg agttcactct ccgacacacg gacagcaacc aggcattcgc accagaggga
     7081 tgcagactgg agcaaaaggt tctactgtcg ctcattcagc aaatatccgc tgacatgacc
     7141 aatcacaatg agcttaagca gagatatctc aacgaggcac tgttggccat caatatggca
     7201 gatcccatta cccgcgagca cgctcctaaa gtgctcaccg agctgtatcg caattgtcag
     7261 cagttcatta agaacagccc caagaattcg cagttcagca acgtccgtct gctgatgaag
     7321 gcaatcataa cgtaccgcga tcagctaaaa agtctagagg gcccgcggtt cgaaggtaag
     7381 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
     7441 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctaaggcctg agctcgctga
     7501 tcagcctcga tcgaggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     7561 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     7621 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     7681 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     7741 atggctgatt atgatcagtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     7801 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     7861 actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg
     7921 gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca
     7981 agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg
     8041 catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc
     8101 cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat
     8161 gctgtccagg caggtagatg acgaccatca gggacagctt caaggccagc aaaaggccag
     8221 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8281 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8341 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8401 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8461 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8521 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8581 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8641 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8701 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8761 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8821 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8881 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8941 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     9001 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9061 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9121 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9181 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9241 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9301 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat
     9361 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9421 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9481 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9541 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9601 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt
     9661 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9721 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9781 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9841 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9901 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9961 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
    10021 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt
    10081 aattctatat tctaaaaaca caaatgatac ttctaaaaaa aaat
//
