LOCUS       sequence-362946-        9454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmCup.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9454)
  AUTHORS   Tritschler F, Eulalio A, Helms S, Schmidt S, Coles M, Weichenrieder 
            O, Izaurralde E, Truffault V
  TITLE     Similar modes of interaction enable Trailer Hitch and EDC3 to 
            associate with DCP1 and Me31B in distinct protein complexes.
  JOURNAL   Mol Cell Biol. 2008 Nov . 28(21):6695-708.
  PUBMED    18765641
REFERENCE   2  (bases 1 to 9454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2008 Nov . 28(21):6695-708."
COMMENT     Sequence Label: pAc5.1B-EGFP-DmCup_C
FEATURES             Location/Qualifiers
     source          1..9454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             join(2585..2587,2588..2590,2591..3301)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2585..2587,2588..2590,2591..3301)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2891..2910)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3238..3259
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             6760..6801
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6811..6828
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6851..6868)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6901..7035
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6925..6944)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6979..6998
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7636..8224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7716..7735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8395..9186,9187..9255))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9018..9037
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9256..9360)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9428..9446
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtc agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 catcatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     2641 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     2701 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     2761 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     2821 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     2881 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2941 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3001 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3061 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3121 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3181 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3241 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3301 gccaagcttg aattctgcag atatcatgga ccctatgaaa gaggaggcga tcgaattgaa
     3361 tggccatgct atgcaaatgg ccgaagctga gcaagaaaat ggcgctggcg ccctgaaaat
     3421 tgccaccaat gcgggtgcaa ccgatcgtcc agcgcatcag cagcttccgc tgccagtcga
     3481 agatcagcag gatgaggtgc tgacccccgc tgagaagggc aagttcgagt accctccccc
     3541 tccaccgcca ccaactccag tccaggctcc gctagccacc aaggccaccg ctctgaatgc
     3601 cagtcaagag catgacgacg acgaggccaa ctccgagaag tgggaggatc cgtgcgctcc
     3661 gcccccgcct cctccgctgc cgactagcgc tttcctggcc accggactgg gctacctcaa
     3721 gctgcccgcc ttcaagctga aggacgcact ggagaaggcc atcaccaagt tggaggccaa
     3781 caagcggacg ctaaaggcca gccctgagtc ttctcgttcc atcaaaaaca agaatgtggt
     3841 ggccctggag atgttaccca ggcgctcaaa cccagaaacg atcggcgatg gatccatgtt
     3901 ggcatccaca tccacggctg ttatgctgca gaccaaaaag cccgccgtta ttgtggagat
     3961 ggagaggcgt tgtaagatca tcaatttact ggccaaacag aatcaaatcc tcgaatccat
     4021 ttcgggggag gccattccga tgcatggacc ctcgaagcat ctgcacgagg acgagggtct
     4081 gaccctgcaa gttctctcgg cccgagcatc cacaccgtac acacagccct ccagcatgtt
     4141 gtcctgcacg gccgttagtt gtgacctgga gcacgattcg ccccggaagc aagtggccag
     4201 caaggaggcc gtgccggagc agcagtctag tcaggtgcag caaaagcgtc cacccagcac
     4261 gggcattcac aagccaggaa gtctgcgagc acccaaggct gttcgtccta ctactgctcc
     4321 ggtggtcagc agcaagccgg tcaagagcta tacccgctcc cgcctgatgg acattcgcaa
     4381 cgggatgttc aatgccctga tgcacaggtc caaggagagc tttgtgatgc ctcgcatcgc
     4441 cacctgcgac gacattgagt tggagggaag gcttcgccgc atgaatattt ggcgcacctc
     4501 ggacggcaca agattccgga cccgctccac cacagctaac ttaaacatga acaacaacaa
     4561 caacaatgag tgcatgtctg ccttcttcaa aaacaaaaat aagccgaacc taatcagtga
     4621 cgagtccatc atccagagcc aaccgcccca gccgcaaacg gagttccaag atccggccat
     4681 tgttaaccaa agacgcattg gcagcggtcg cttaaaccat tccaagtggg gttacaacga
     4741 cgaagactac cactcgtacc acaatggcaa gtcgcagcat atggaggagg tcaactcgaa
     4801 gaacagcaaa aacatgacgg tgctgcagtt cttcgacaac ggcgagatca gcagtcagcc
     4861 tcaaagacgg ccgaacactc ctgtgatggg gatgtctatc aatcgatccg agaacgacac
     4921 cctgcactcg aacgagtcca gtgaagattt gagtcgcgcc aacgagaact atgtaaagcg
     4981 cgtaatgtct ggattcctgg ttgtatcgaa gcccaagtca cgagatgtcg aggatagaca
     5041 tcaccggcgg tacaggaacc agaacgagga gcctgagtgg tttagctgtg gacccacgtc
     5101 tagactggac actatagagt tgtgtgggtt cgatgaggac gaggagaaaa tgctaaagga
     5161 gggcaacaaa aaccacggat taggcgaaac agagcgagaa acgtcaaaac aaaagatgga
     5221 ccataagtac aaatggacgc atgcggagcc gatgggtcga agcaaataca tgcccaaaca
     5281 cgacactaac aataatcata acgtggagaa catgaataat gttatggcta ccgagcatca
     5341 gcaacaaaag gaagagaaac gcccaggcag tggccgttcg tttcagtttg ataagttcaa
     5401 tcaaagccaa cagaattacg agagtagcag ttatgttaat caccaacagc cgccgcaaac
     5461 gcagcctcag caaatgcagc agcaatcgaa cacaaacacg aacaactcta aattcatgtc
     5521 attctttgcc aatgagggga actcctcgtc ttcttcgctg aacgagtttt tcaagcaggc
     5581 cattaaccag ggtcatggca acaatccgga gcagcccaaa tcgttgggcc acatcggaca
     5641 gatgccttcg gtggatcagc tggaggccaa atggcgtcga aactcactga acaatgttgg
     5701 cgagactgcc aacaagcaga ctgataactt ccagaagcta attgggtctc tttcttcggc
     5761 taagccacag tcacaggcgg ttggctatga tgccatctcc aactttataa tgcagcaaca
     5821 gcagtaccag cagcagcaac agaagcagca cctcatcata cagcagcagc agcaacagac
     5881 cgcctttttg gcgagcctgc agctgaaggc gatcctcggc cgggccgaca cccaattgct
     5941 actgctccgt ctgactaaag gggaaatatc caagcacgga ttgctggtgc agttggccaa
     6001 cccacgcttg acggacatgg atcgcgaggc catcactgct gtattgcagt tcaccaacac
     6061 ccagcagcag cagcagcagc acaagcagca gctggacatg ctctcaagca cggtcatcgc
     6121 cagccagttg cagaatctcc acaacttggc cattgtccag cagactcttg cagccaggca
     6181 gcagccgcag cataatccac agacgcaggc gccgcaccaa ctgtcgcagg aggacttgca
     6241 agcgcatgcc aatgtgataa tgcgaaacgc cgtaatgaag cgaaaaattg aggagcagac
     6301 ctcgaagctt atcaatggcg gtgccaagca tcaggcacag cagcaatatc taaaccgtgg
     6361 ccagcaacgc caggctcgac cagatgccaa ctcgaatgcc ttgctccatg ccttgatctc
     6421 tggcggtggc aataaccacg cctctggtta tcctatgaaa ggacagccgc agcagcatca
     6481 ttccaatttg agattcggtg ataaccaaaa cttccaatct tttgagtcca accagccgca
     6541 cttcgccacg cagtacaagc agcaatacca acagtctcag cagcaacatc cccatcagca
     6601 gccccaacag ctgaattcgc ttcatcagaa caacgctgga gctgtcaata gcttcaataa
     6661 ggcccaaatg caagctcaat cagctatttc catgctgccc aacagcgggg atgagtttca
     6721 ttaagcggcc gctcgagtct agagggcccg cggttcgaag gtaagcctat ccctaaccct
     6781 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc
     6841 cgctgatcag cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag
     6901 gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     6961 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     7021 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     7081 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat
     7141 cagtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     7201 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     7261 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     7321 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     7381 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     7441 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     7501 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     7561 agatgacgac catcagggac agcttcaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7621 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7681 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7741 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7801 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7861 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7921 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7981 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     8041 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     8101 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8161 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8221 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8281 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8341 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8401 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8461 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8521 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8581 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8641 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8701 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8761 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8821 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8881 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8941 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     9001 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     9061 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9121 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9181 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9241 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9301 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9361 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     9421 ctataaaaat aggcgtatca cgaggccctt tcgt
//
