LOCUS       sequence-362947-        9860 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Buganim Y, Itskovich E, Hu YC, Cheng AW, Ganz K, Sarkar S, Fu D, 
            Welstead GG, Page DC, Jaenisch R
  TITLE     Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like 
            Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2012 Sep 7;11(3):373-86.
  PUBMED    22958931
REFERENCE   2  (bases 1 to 9860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2012-09-7"; volume: "11(3)"; pages: "373-86"
COMMENT     Sequence Label: FUW-TetO-Gata4
FEATURES             Location/Qualifiers
     source          1..9860
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        133..512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        514..712
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             730..910
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    957..1082
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1575..1808
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1993..2037
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2335..2452
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2509..2527
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2551..2569
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2593..2611
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2635..2653
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2677..2695
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2719..2737
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2770..2808
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2805..2829
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4447..5035
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4500..4520)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4918..4929)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5038..5054)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5039..5055)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5560..5740
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5766..5783)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5772..5996
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6042..6470
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6129..6148)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6339..6360
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6479..6499)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6484..6813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6664..6799
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6726..6745
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6861..6908
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6927..7301
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7431..7552
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7468..7487)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7522..7541
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7601..7617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7601..7617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7614..7636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7625..7641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7649..7655,7656..7673,7674..7679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7694..7715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7832..7849)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8003..8591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8083..8102)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8762..9553,9554..9622))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9385..9404
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9623..9727)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9802..9821)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
       61 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
      121 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
      181 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
      241 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
      301 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
      361 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
      421 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
      481 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
      541 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
      601 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
      661 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt
      721 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
      781 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
      841 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
      901 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
      961 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     1021 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     1081 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     1141 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     1201 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     1261 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     1321 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     1381 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     1441 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     1501 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     1561 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     1621 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     1681 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     1741 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     1801 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     1861 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     1921 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     1981 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     2041 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     2101 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     2161 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     2221 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     2281 ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa
     2341 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     2401 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     2461 ttacagggac agcagagatc cagtttggtt agatctcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2701 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agctcggtac
     2761 ccgggtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
     2821 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac
     2881 cgatccagcc tccgcggccc cgaattcgcc ctttttgggt ttttgttttg ttttgttttg
     2941 ttttgatttt tggtgacagt tccgcacacc cgcattctag ttcttgtctg cctcgtgctc
     3001 agagcttggg gcgatgtacc aaagcctggc catggccgcc aaccacggcc ccccgcccgg
     3061 cgcctacgaa gcaggtggcc ctggcgcctt catgcacagc gcgggcgccg cgtcctcgcc
     3121 cgtctacgtg cccactccgc gggtgccgtc ctctgtgctg ggcctgtcct acctgcaggg
     3181 cggtggcagt gccgctgcag ctggaaccac ctcgggtggc agctccgggg ccggcccgtc
     3241 gggtgcaggg cctgggaccc agcagggtag ccctggctgg agccaagctg gagccgaggg
     3301 agccgcctac accccgccgc ccgtgtcccc gcgcttctct ttcccgggga ctactgggtc
     3361 cctggcggcc gctgccgccg ctgccgcagc ccgggaagct gcagcctacg gcagtggcgg
     3421 cggggcggcg ggcgctggtc tggctggccg agagcagtac gggcgtccgg gcttcgccgg
     3481 ctcctactcc agcccctacc cagcctacat ggccgacgtg ggagcatcct gggccgcagc
     3541 cgctgccgcc tctgccggcc ccttcgacag cccagtcctg cacagcctgc ctggacgggc
     3601 caaccctgga agacacccca atctcgtaga tatgtttgat gacttctcag aaggcagaga
     3661 gtgtgtcaat tgtggggcca tgtccacccc actctggagg cgagatggga cgggacacta
     3721 cctgtgcaat gcctgtggcc tctatcacaa gatgaacggc atcaaccggc ccctcattaa
     3781 gcctcagcgc cgcctgtccg cttcccgccg ggtaggcctc tcctgtgcca actgccagac
     3841 taccaccacc acgctgtggc gtcgtaatgc cgagggtgag cctgtatgta atgcctgcgg
     3901 cctctacatg aagctccatg gggttcccag gcctcttgca atgcggaagg aggggattca
     3961 aaccagaaaa cggaagccca agaacctgaa taaatctaag acgccagcag gtcctgctgg
     4021 tgagaccctc cctccctcca gtggtgcctc cagcggtaac tccagcaatg ccactagcag
     4081 cagcagcagc agtgaagaga tgcgccccat caagacagag cccgggctgt catctcacta
     4141 tgggcacagc agctccatgt cccagacatt cagtactgtg tccggccacg ggccctccat
     4201 ccatccagtg ctgtctgctc tgaagctgtc cccacaaggc tatgcatctc ctgtcactca
     4261 gacatcgcag gccagctcca agcaggactc ttggaacagc ctggtcctgg ctgacagtca
     4321 tggggacata atcaccgcgt aatcagcgcc cccccttccc tcttcaaatt cctgctcgga
     4381 cttgggacgt gggggccagc aaagtaaaag gctgggaagg gcgaattcga tatcaagctt
     4441 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4501 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4561 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4621 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4681 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4741 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4801 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
     4861 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4921 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4981 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga
     5041 taccgtcgac ctcgagacct agaaaaacat ggagcaatca caagtagcaa tacagcagct
     5101 accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg ttttccagtc
     5161 acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5221 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatatcctt
     5281 gatctgtgga tctaccacac acaaggctac ttccctgatt ggcagaacta cacaccaggg
     5341 ccagggatca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcaa
     5401 gagaaggtag aagaagccaa tgaaggagag aacacccgct tgttacaccc tgtgagcctg
     5461 catgggatgg atgacccgga gagagaagta ttagagtgga ggtttgacag ccgcctagca
     5521 tttcatcaca tggcccgaga gctgcatccg gactgtactg ggtctctctg gttagaccag
     5581 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     5641 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     5701 tccctcagac ccttttagtc agtgtggaaa atctctagca gggcccgttt aaacccgctg
     5761 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     5821 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     5881 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     5941 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     6001 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     6061 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6121 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6181 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6241 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6301 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6361 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6421 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     6481 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6541 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     6601 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     6661 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6721 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     6781 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     6841 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     6901 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     6961 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     7021 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     7081 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     7141 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     7201 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     7261 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     7321 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     7381 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     7441 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     7501 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     7561 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     7621 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     7681 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     7741 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     7801 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     7861 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     7921 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7981 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     8041 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     8101 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     8161 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     8221 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     8281 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     8341 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     8401 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     8461 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     8521 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     8581 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8641 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8701 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8761 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8821 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8881 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8941 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     9001 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     9061 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     9121 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     9181 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     9241 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     9301 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     9361 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     9421 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     9481 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     9541 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9601 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     9661 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     9721 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatctcccga
     9781 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     9841 gctccctgct tgtgtgttgg
//
