LOCUS       sequence-362948-        7208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Insect Expression of DmPABPC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7208)
  TITLE     Izaurralde lab plasmids
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pAc5.1B-DmPABPC1-V5His6_D
FEATURES             Location/Qualifiers
     source          1..7208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             4514..4555
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             4565..4582
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4605..4622)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4655..4789
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4679..4698)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4733..4752
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(5390..5978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5470..5489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6149..6940,6941..7009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6772..6791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7010..7114)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7182..7200
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatggct tctctatacg tcggtgatct cccacaggac gtcaacgaat cgggactttt
     2641 tgacaagttc tcgtctgcta gtccagtgct gtccattcgt gtctgccgcg atgtgattac
     2701 ccgtcgctcg ttgggctatg cctacgtcaa cttccagcag ccagccgatg ctgagcgtgc
     2761 tttggacacc atgaactttg acctggttcg caacaagccc attcgcatta tgtggtctca
     2821 gcgtgatccg tctcttcgtc gctccggtgt gggcaacgtg ttcatcaaga acttagatag
     2881 ggccatcgac aacaaggcaa tctacgacac tttctccgcc ttcggcaaca ttttaagctg
     2941 caaggtagcc accgatgaaa agggcaactc aaagggctat ggattcgtcc acttcgagac
     3001 cgaggaggcc gcaaacacgt ccatcgacaa agtcaatggc atgttgctta acggcaagaa
     3061 ggtctacgtg ggtaagttca tcccgcgcaa ggagcgcgag aaggagctgg gcgagaaggc
     3121 taagctcttc accaatgtat atgtgaagaa cttcactgag gatttcgacg atgaaaaact
     3181 gaaggaattc tttgagccct acggcaagat aaccagctac aaggtcatgt ccaaggagga
     3241 tggcaagagc aagggcttcg gattcgttgc attcgagaca acagaggctg ctgaggcggc
     3301 cgttcaggct ctcaatggca aggacatggg agagggcaag tccctgtacg tggctcgtgc
     3361 ccagaagaag gccgaacgcc agcaggagct gaagcgcaag ttcgaggaac tgaagcagaa
     3421 gcgtcacgag tcagtgttcg gcgttaactt gtacgttaag aacctggacg acacaatcga
     3481 tgacgatcgt ctgcgcatcg cgttctctcc atacggcaac atcacatcgg ccaaggtcat
     3541 gaccgatgag gagggtcgct ccaagggatt cggcttcgtg tgcttcaatg ccgcaagcga
     3601 ggctacctgc gccgtcaccg agctgaacgg tcgcgtcgtc ggcagtaagc cgttgtacgt
     3661 tgctttggcc caaaggaagg aggagcgcaa ggctcatctc gcctcgcagt acatgcgtca
     3721 catgaccggc atgcgtatgc agcagctggg acagatctac cagcccaacg cggctagcgg
     3781 cttctttgtg ccgacccttc cgtcaaatca gcgtttcttc ggttcccagg tggccactca
     3841 gatgagaaac acaccccgct gggtgcccca ggtgcgtccc ccagcagcca tacagggtgt
     3901 ccaggccgga gctgccgctg ctggtggctt ccagggcaca gccggtgcgg tgcccactca
     3961 gttccgttcg gctgctgctg gcgctcgcgg ggcacagcct caggtgcagg gcacacacgc
     4021 cgccgctgct gcggctaaca acatgcgcaa cactggagca cgcgctatta ctggccagca
     4081 aactgcagca cccaacatgc agatccccgg agcccaaatc gctggcggtg cccagcagcg
     4141 tacttccaac tataagtaca cctcaaacat gcgtaatcct ccagtgccac agttgcatca
     4201 gacccagcca attccacagc aattgcaagg aaagaattct gagaagctca ttgcctcgtt
     4261 gctggccaac gccaagccgc aggagcagaa acagatcctg ggcgagcgtc tgtatccgat
     4321 gattgaacat atgcacgcca atttggctgg taagatcacc ggcatgttgt tggagatcga
     4381 aaactctgag ctcttgcaca tgatcgagga tcaggaggcc cttaaggcca aggtggagga
     4441 ggctgtggcc gtgcttcagg tgcaccgcgt caccgagccc gccaactcga gtctagaggg
     4501 cccgcggttc gaaggtaagc ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac
     4561 cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc
     4621 taaggcctga gctcgctgat cagcctcgat cgaggatcca gacatgataa gatacattga
     4681 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
     4741 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
     4801 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     4861 aaacctctac aaatgtggta tggctgatta tgatcagtcg accgatgccc ttgagagcct
     4921 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     4981 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg
     5041 gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa
     5101 tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga
     5161 agcaggccat tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg
     5221 cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga
     5281 tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc
     5341 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5401 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5461 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5521 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5581 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5641 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5701 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5761 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5821 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5881 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5941 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6001 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6061 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6121 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6181 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     6241 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6301 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6361 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6421 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt
     6481 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6541 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6601 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6661 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6721 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
     6781 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6841 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6901 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6961 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     7021 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     7081 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     7141 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     7201 cctttcgt
//
