LOCUS       sequence-362954-         570 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Genomic targeting of SNAP tag at H3f3b C-terminal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Xu X, Duan S, Hua X, Li Z, He R, Zhaang Z
  TITLE     Stable inheritance of H3.3-containing nucleosomes during mitotic 
            cell divisions.
  JOURNAL   Nat Commun. 2022 May 6;13(1):2514. doi: 10.1038/s41467-022-30298-4.
  PUBMED    35523900
REFERENCE   2  (bases 1 to 570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-05-6"; volume: "13(1):2514. doi"; pages: " 
            10.1038/s41467-022-30298-4"
COMMENT     Sequence Label: pBlueScript II SK-SNAP donor for H3f3b
FEATURES             Location/Qualifiers
     source          1..570
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..570
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for bacterial expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 ggaggaggat ccggaggtgg cagcatggac aaagattgcg aaatgaaacg taccaccctg
       61 gatagcccgc tgggcaaact ggaactgagc ggctgcgaac agggcctgca tgaaattaaa
      121 ctgctgggta aaggcaccag cgcggccgat gcggttgaag ttccggcccc ggccgccgtg
      181 ctgggtggtc cggaaccgct gatgcaggcg accgcgtggc tgaacgcgta ttttcatcag
      241 ccggaagcga ttgaagaatt tccggttccg gcgctgcatc atccggtgtt tcagcaggag
      301 agctttaccc gtcaggtgct gtggaaactg ctgaaagtgg ttaaatttgg cgaagtgatt
      361 agctatcagc agctggcggc cctggcgggt aatccggcgg ccaccgccgc cgttaaaacc
      421 gcgctgagcg gtaacccggt gccgattctg attccgtgcc atcgtgtggt tagctctagc
      481 ggtgcggttg gcggttatga aggtggtctg gcggtgaaag agtggctgct ggcccatgaa
      541 ggtcatcgtc tgggtaaacc gggtctggga
//
