LOCUS       sequence-362956-        7640 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To drive hM3D fused with Myc tag under the control of mouse oxytocin
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Inada K, Hagihara M, Tsujimoto K, Abe T, Konno A, Hirai H, Kiyonari 
            H, Miyamichi K
  TITLE     Plasticity of neural connections underlying oxytocin-mediated 
            parental behaviors of male mice.
  JOURNAL   Neuron. 2022 Apr 12. pii: S0896-6273(22)00304-X. doi: 
            10.1016/j.neuron.2022.03.033.
  PUBMED    35443152
REFERENCE   2  (bases 1 to 7640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2022 Apr 12. pii: S0896-6273(22)00304-X. doi: 
            10.1016/j.neuron.2022.03.033."
COMMENT     Sequence Label: pAAV-OTp-hM3Dq-Myc
FEATURES             Location/Qualifiers
     source          1..7640
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             4545..4574
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4581..4610
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4617..4646
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4682..4803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4693..4712)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4747..4766
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4903..5043
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4903..5032
                     /label=AAV2 ITR
     rep_origin      5118..5573
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5205..5224)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5415..5436
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5590..5609)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5709..5731
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     promoter        5855..5959
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5960..6028,6029..6820)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6178..6197)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6991..7579
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7480..7499
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtagatga gctggtgagc atgtgaagac atgctggcct
      181 aacaggcgcg ttctgcacat attttatgac atgctaattt atgtgcatgg attaccttag
      241 gtgagatcac tatgccctca gaacatgcag gctgtctctc tggggccact ttgggggtca
      301 aactcttggc cttctatgcc cagaatgcca ataggcaatt gtgggtgaga aatgaactga
      361 tttgtagtac ggttccaatc aacctcacca cccacttagc gttgataact cacaccagtg
      421 actcaggaga gctggggaac agggcttgcc ctgctggccc cagccccttg aggagaaggt
      481 tgagcataag gacagagcct aggggctttt caattctctc caggggcaga gactgcatgt
      541 ggtgagctgg tctctgggac actggctgtt acttcttcct ggatgttgca ggacactttc
      601 taagagagcc tgagatgaag tttagaacat gggactggga cccacccctg tggaagagaa
      661 ggggtacggg agcagagcca gtatgcagat ctcagctaac ctaaagccca aagctgaagt
      721 gcagtcaggg gtacccagcc tttgtaccaa aatggaccaa ctgctatatg tagatagctg
      781 ggaaggccag ctagctttct gcagctaaga ccacactccg gcagctgagg acaagtgttt
      841 tcttaaacag gagcctaggt acctggcatg gtggtacacc cagcacgtgg caggcaggtg
      901 gatcactgtg agttcaagcc cagcctggtc tacacagaca gctccaggcc aaccaagagc
      961 ctatctcaaa aaaaaaaaaa aaaaaaaaac aaacggaagt ctagacaatg cttctctttt
     1021 cctttttaaa attgttttag atctatttat tttattttac gtgtctgaat gatctgcctg
     1081 catgtgagta atgcactatt cgtgtgccgg ctgctaggtc agaataagtc cctggctcca
     1141 ctggaattgt tgtaagtggt tgtgagccac cctgtgggtg ctgggaatca aacctgggtc
     1201 ttctgcaaga gtaactctca gccccaagat agcaagatat ttaacatgag gcccagtgga
     1261 tgatcttcag gtcattgagg gcaagccctt gaagtgagta gtgagacctt catctcctct
     1321 ggagaggttt ttgatttctc agccataaaa tgaaggggtt gagtcttccg tgtcttcctg
     1381 ctatgctttg tctgtctgtc tttcttttgt tttggttgat tttggtttta gtttgaagcc
     1441 ctggctgtcc tggaactaac agaaattctc ctgcctctgt ctcctgagtt ctgggattaa
     1501 aggtgtgcac ccccagcacc ctctcttgat atgtatttca ttttaagggg ctggagagat
     1561 gattagcctg cacactgctc tttcagagaa ccaaattttg tcctgggcac ccacataggc
     1621 ctacccatga tcccctttaa tgacagctcc tggggatcca gcaccctctt ctggtcgcca
     1681 aggaaacctg cgtgcacaaa tgtacacaca caaaattaaa attgaaatgc aaaaactgtt
     1741 cccaaaatgt actgctacca tcatgcgggg gacttgcccc acccaacgtc gctcacacac
     1801 taggcaagta ctctgctact gggctgcatg taacaccctt cccatgcaga cctatgcaga
     1861 cctgcagccc aaacctgaaa tgtacccaga gctgcccaat tgattgcacc aaaatgggcc
     1921 aaccattata tgtggccacc tgagaagggt atgaccttgg cacaaatggc ttgctgtagc
     1981 tgaggccacc tgttggccac actccagcag ctgatggcca ctgttttctc aaacaggagt
     2041 ctaggcacct agtgtggtag tggataacta aactcagcat ttgggagaca gaagcagatg
     2101 gagcgctgtg agttcaaggc cagcctggtc tacacagcag gttctaatac agagttttaa
     2161 atactagtgt aattttcctt ttgctgtaat ttttctttct ttttatttta atttggtctg
     2221 ttaactgtgt tttggttttg atctctactg agactttgtt ttacgggcta gttagaagag
     2281 ctgaggtgca ttcagagatt gaacaagacg ccatcttttt ccccatctaa gtttcaggtc
     2341 catgtaaggg ctccctcact cacttgccct atcctgcctt attctgagat attggatatc
     2401 tgtgaaaaac agctcctggc tagggcgcac ctccaacccc tcccaagtct ctctagcctc
     2461 ttgtagccta ggccacccct tccaggctgc ttctcttttg agttccaggt cattagcaga
     2521 gacgatgacc ttgaccctag cccagaccct gcaaatgaag ggcctgcctc taaacagcgt
     2581 ggaacaattt gacccaagag accttctgtg accagtcatg ctgtcaccct ctttagacag
     2641 tgctccacca tggcagtgcc cagacataaa aaggtcggtc tgggccggag aaaccatcac
     2701 ctacagcgga tctcagactg agcaccatcg ccgaattcgc caccatgacc ttgcacaata
     2761 acagtacaac ctcgcctttg tttccaaaca tcagctcctc ctggatacac agcccctccg
     2821 atgcagggct gcccccggga accgtcactc atttcggcag ctacaatgtt tctcgagcag
     2881 ctggcaattt ctcctctcca gacggtacca ccgatgaccc tctgggaggt cataccgtct
     2941 ggcaagtggt cttcatcgct ttcttaacgg gcatcctggc cttggtgacc atcatcggca
     3001 acatcctggt aattgtgtca tttaaggtca acaagcagct gaagacggtc aacaactact
     3061 tcctcttaag cctggcctgt gccgatctga ttatcggggt catttcaatg aatctgttta
     3121 cgacctacat catcatgaat cgatgggcct tagggaactt ggcctgtgac ctctggcttg
     3181 ccattgactg cgtagccagc aatgcctctg ttatgaatct tctggtcatc agctttgaca
     3241 gatacttttc catcacgagg ccgctcacgt accgagccaa acgaacaaca aagagagccg
     3301 gtgtgatgat cggtctggct tgggtcatct cctttgtcct ttgggctcct gccatcttgt
     3361 tctggcaata ctttgttgga aagagaactg tgcctccggg agagtgcttc attcagttcc
     3421 tcagtgagcc caccattact tttggcacag ccatcgctgg tttttatatg cctgtcacca
     3481 ttatgactat tttatactgg aggatctata aggaaactga aaagcgtacc aaagagcttg
     3541 ctggcctgca agcctctggg acagaggcag agacagaaaa ctttgtccac cccacgggca
     3601 gttctcgaag ctgcagcagt tacgaacttc aacagcaaag catgaaacgc tccaacagga
     3661 ggaagtatgg ccgctgccac ttctggttca caaccaagag ctggaaaccc agctccgagc
     3721 agatggacca agaccacagc agcagtgaca gttggaacaa caatgatgct gctgcctccc
     3781 tggagaactc cgcctcctcc gacgaggagg acattggctc cgagacgaga gccatctact
     3841 ccatcgtgct caagcttccg ggtcacagca ccatcctcaa ctccaccaag ttaccctcat
     3901 cggacaacct gcaggtgcct gaggaggagc tggggatggt ggacttggag aggaaagccg
     3961 acaagctgca ggcccagaag agcgtggacg atggaggcag ttttccaaaa agcttctcca
     4021 agcttcccat ccagctagag tcagccgtgg acacagctaa gacttctgac gtcaactcct
     4081 cagtgggtaa gagcacggcc actctacctc tgtccttcaa ggaagccact ctggccaaga
     4141 ggtttgctct gaagaccaga agtcagatca ctaagcggaa aaggatgtcc ctggtcaagg
     4201 agaagaaagc ggcccagacc ctcagtgcga tcttgcttgc cttcatcatc acttggaccc
     4261 catacaacat catggttctg gtgaacacct tttgtgacag ctgcataccc aaaacctttt
     4321 ggaatctggg ctactggctg tgctacatca acagcaccgt gaaccccgtg tgctatgctc
     4381 tgtgcaacaa aacattcaga accactttca agatgctgct gctgtgccag tgtgacaaaa
     4441 aaaagaggcg caagcagcag taccagcaga gacagtcggt catttttcac aagcgcgcac
     4501 ccgagcaggc cttgaaggat cccccggtcg ccaccagatc cttagagcag aaactcatta
     4561 gcgaggagga cctgaacagc gaacagaaac tcatttccga agaggatctc aactccgagc
     4621 agaagctgat cagcgaggag gacctgagat cctgagtcga cgccgcttcg agcagacatg
     4681 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     4741 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     4801 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     4861 ttttaaagca agtaaaacct ctacaaatgt ggtagcggcc gcaggaaccc ctagtgatgg
     4921 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
     4981 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
     5041 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
     5101 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     5161 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
     5221 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     5281 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     5341 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     5401 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
     5461 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
     5521 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
     5581 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     5641 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     5701 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     5761 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     5821 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     5881 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     5941 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6001 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6061 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6121 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6181 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6241 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6301 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6361 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6421 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6481 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6541 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6601 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6661 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6721 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     6781 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     6841 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6901 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6961 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7021 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7081 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7141 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7201 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7261 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7321 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7381 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7441 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7501 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7561 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7621 ctggcctttt gctcacatgt
//
