LOCUS       sequence-362957-        7350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To drive mCherry under the control of mouse oxytocin promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Inada K, Hagihara M, Tsujimoto K, Abe T, Konno A, Hirai H, Kiyonari 
            H, Miyamichi K
  TITLE     Plasticity of neural connections underlying oxytocin-mediated 
            parental behaviors of male mice.
  JOURNAL   Neuron. 2022 Apr 12. pii: S0896-6273(22)00304-X. doi: 
            10.1016/j.neuron.2022.03.033.
  PUBMED    35443152
REFERENCE   2  (bases 1 to 7350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2022 Apr 12. pii: S0896-6273(22)00304-X. doi: 
            10.1016/j.neuron.2022.03.033."
COMMENT     Sequence Label: pAAV-OTp-mCherry
FEATURES             Location/Qualifiers
     source          1..7350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      2739..2748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2745..3455
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2890..2908)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3158..3177
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    3479..4067
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3532..3552)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3950..3961)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    4091..4567
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4290..4309)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   4613..4753
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4613..4742
                     /label=AAV2 ITR
     rep_origin      4828..5283
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4915..4934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5125..5146
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5300..5319)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5419..5441
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5479..5497)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5565..5669
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5670..5738,5739..6530)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5888..5907)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6701..7289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7190..7209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtagatga gctggtgagc atgtgaagac atgctggcct
      181 aacaggcgcg ttctgcacat attttatgac atgctaattt atgtgcatgg attaccttag
      241 gtgagatcac tatgccctca gaacatgcag gctgtctctc tggggccact ttgggggtca
      301 aactcttggc cttctatgcc cagaatgcca ataggcaatt gtgggtgaga aatgaactga
      361 tttgtagtac ggttccaatc aacctcacca cccacttagc gttgataact cacaccagtg
      421 actcaggaga gctggggaac agggcttgcc ctgctggccc cagccccttg aggagaaggt
      481 tgagcataag gacagagcct aggggctttt caattctctc caggggcaga gactgcatgt
      541 ggtgagctgg tctctgggac actggctgtt acttcttcct ggatgttgca ggacactttc
      601 taagagagcc tgagatgaag tttagaacat gggactggga cccacccctg tggaagagaa
      661 ggggtacggg agcagagcca gtatgcagat ctcagctaac ctaaagccca aagctgaagt
      721 gcagtcaggg gtacccagcc tttgtaccaa aatggaccaa ctgctatatg tagatagctg
      781 ggaaggccag ctagctttct gcagctaaga ccacactccg gcagctgagg acaagtgttt
      841 tcttaaacag gagcctaggt acctggcatg gtggtacacc cagcacgtgg caggcaggtg
      901 gatcactgtg agttcaagcc cagcctggtc tacacagaca gctccaggcc aaccaagagc
      961 ctatctcaaa aaaaaaaaaa aaaaaaaaac aaacggaagt ctagacaatg cttctctttt
     1021 cctttttaaa attgttttag atctatttat tttattttac gtgtctgaat gatctgcctg
     1081 catgtgagta atgcactatt cgtgtgccgg ctgctaggtc agaataagtc cctggctcca
     1141 ctggaattgt tgtaagtggt tgtgagccac cctgtgggtg ctgggaatca aacctgggtc
     1201 ttctgcaaga gtaactctca gccccaagat agcaagatat ttaacatgag gcccagtgga
     1261 tgatcttcag gtcattgagg gcaagccctt gaagtgagta gtgagacctt catctcctct
     1321 ggagaggttt ttgatttctc agccataaaa tgaaggggtt gagtcttccg tgtcttcctg
     1381 ctatgctttg tctgtctgtc tttcttttgt tttggttgat tttggtttta gtttgaagcc
     1441 ctggctgtcc tggaactaac agaaattctc ctgcctctgt ctcctgagtt ctgggattaa
     1501 aggtgtgcac ccccagcacc ctctcttgat atgtatttca ttttaagggg ctggagagat
     1561 gattagcctg cacactgctc tttcagagaa ccaaattttg tcctgggcac ccacataggc
     1621 ctacccatga tcccctttaa tgacagctcc tggggatcca gcaccctctt ctggtcgcca
     1681 aggaaacctg cgtgcacaaa tgtacacaca caaaattaaa attgaaatgc aaaaactgtt
     1741 cccaaaatgt actgctacca tcatgcgggg gacttgcccc acccaacgtc gctcacacac
     1801 taggcaagta ctctgctact gggctgcatg taacaccctt cccatgcaga cctatgcaga
     1861 cctgcagccc aaacctgaaa tgtacccaga gctgcccaat tgattgcacc aaaatgggcc
     1921 aaccattata tgtggccacc tgagaagggt atgaccttgg cacaaatggc ttgctgtagc
     1981 tgaggccacc tgttggccac actccagcag ctgatggcca ctgttttctc aaacaggagt
     2041 ctaggcacct agtgtggtag tggataacta aactcagcat ttgggagaca gaagcagatg
     2101 gagcgctgtg agttcaaggc cagcctggtc tacacagcag gttctaatac agagttttaa
     2161 atactagtgt aattttcctt ttgctgtaat ttttctttct ttttatttta atttggtctg
     2221 ttaactgtgt tttggttttg atctctactg agactttgtt ttacgggcta gttagaagag
     2281 ctgaggtgca ttcagagatt gaacaagacg ccatcttttt ccccatctaa gtttcaggtc
     2341 catgtaaggg ctccctcact cacttgccct atcctgcctt attctgagat attggatatc
     2401 tgtgaaaaac agctcctggc tagggcgcac ctccaacccc tcccaagtct ctctagcctc
     2461 ttgtagccta ggccacccct tccaggctgc ttctcttttg agttccaggt cattagcaga
     2521 gacgatgacc ttgaccctag cccagaccct gcaaatgaag ggcctgcctc taaacagcgt
     2581 ggaacaattt gacccaagag accttctgtg accagtcatg ctgtcaccct ctttagacag
     2641 tgctccacca tggcagtgcc cagacataaa aaggtcggtc tgggccggag aaaccatcac
     2701 ctacagcgga tctcagactg agcaccatcg ccgaattcgc caccatggtg agcaagggcg
     2761 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     2821 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     2881 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     2941 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     3001 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     3061 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     3121 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     3181 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     3241 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     3301 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     3361 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     3421 actccaccgg cggcatggac gagctgtaca agtaaaagct tatatgcggc cgatcgataa
     3481 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3541 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3601 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3661 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3721 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3781 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3841 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3901 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     3961 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     4021 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtag
     4081 atcgatctac gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt
     4141 tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga
     4201 ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt
     4261 tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc
     4321 acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc
     4381 tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg
     4441 gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat
     4501 ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc
     4561 tgtccttctg attttgtagg taaccacgtg cggaccgaac tagtgcggcc gcaggaaccc
     4621 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4681 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4741 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     4801 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     4861 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
     4921 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4981 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     5041 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     5101 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
     5161 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
     5221 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     5281 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5341 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5401 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5461 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5521 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5641 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5701 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5761 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5821 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5881 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5941 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6001 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6061 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6121 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6181 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6241 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6301 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6361 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6421 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6481 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6541 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6601 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6661 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6721 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6781 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6841 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6901 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6961 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7021 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7081 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7141 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7201 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7261 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7321 tggccttttg ctggcctttt gctcacatgt
//
