LOCUS       sequence-363015-        8464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of spCas9, gRNA targeting the end of mouse Nefl gene and 
            donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression 
            and click chemistry labeling of endogenous NFL..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8464)
  AUTHORS   Arsic A, Hagemann C, Stajkovic N, Schubert T, Nikic-Spiegel I
  TITLE     Minimal genetically encoded tags for fluorescent protein labeling in
            living neurons.
  JOURNAL   Nat Commun. 2022 Jan 14;13(1):314. doi: 10.1038/s41467-022-27956-y.
  PUBMED    35031604
REFERENCE   2  (bases 1 to 8464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2022-01-14"; volume: "13(1):314. doi"; pages: " 
            10.1038/s41467-022-27956-y"
COMMENT     Sequence Label: pORANGE_NFL linker(A6TAG)-3xFLAG KI
FEATURES             Location/Qualifiers
     source          1..8464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(340..405)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_RNA        complement(542..617)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(638..886)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(696..715)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(866..886)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     rep_origin      complement(948..1536)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1028..1047)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1707..2498,2499..2567))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2330..2349
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2568..2672)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2740..2758
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2796..2818)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2918..2937
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2954..3409)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3091..3112)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3303..3322
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3484..3624
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3495..3624
                     /label=AAV2 ITR
     polyA_signal    3633..3840
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3829..3846
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3886..3906)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(3910..8010)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(3910..8010)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(8020..8040)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8044..8070)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          8091..8318
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
ORIGIN
        1 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
       61 acaatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
      121 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
      181 atggaaagtc cctattggcg ttactattga cgtcaatggg cgggggtcgt tgggcggtca
      241 gccaggcggg ccatttaccg taagttatgt aacgggtacc tctggacgcg tcctaggacg
      301 cgtcctagga tccgagtgct ggagaggagc aggtggttac ttgtcatcgt catccttgta
      361 atcgatgtca tgatctttat aatcaccgtc atggtctttg tagtcctagc ttccagcgct
      421 accgagtgct ggagaggagc aggtggaagc ttctagagcc atttgtctgc agaattggcg
      481 cacgcgctaa aaacggacta gccttatttt aacttgctat ttctagctct aaaacaaaaa
      541 agcaccgact cggtgccact ttttcaagtt gataacggac tagccttatt ttaacttgct
      601 atttctagct ctaaaaccct gctcctctcc agcactcggt gtttcgtcct ttccacaaga
      661 tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga tagtccattt
      721 taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta cgtcacgtat
      781 tttgtactaa tatctttgtg tttacagtca aattaattcc aattatctct ctaacagcct
      841 tgtatcgtat atgcaaatat gaaggaatca tgggaaatag gccctcacat gtgagcaaaa
      901 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      961 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1021 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1081 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1141 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1201 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1261 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1321 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1381 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1441 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1501 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1561 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1621 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1681 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1741 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1801 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcggct tccacgctca
     1861 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1921 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1981 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2041 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2101 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2161 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2221 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2281 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2341 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2401 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     2461 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     2521 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     2581 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2641 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2701 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     2761 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     2821 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     2881 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     2941 agagtgcacc ataaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     3001 taaatcagct cattttttaa ccaatagacc gaaatcggca aaatccctta taaatcaaaa
     3061 gaatagcccg agatagagtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     3121 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     3181 gaaccatcac ccaaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     3241 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     3301 gaagggaaga aagcgaaagg agcgggcgct aaggcgctgg caagtgtagc ggtcacgctg
     3361 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgta ctatggttgc
     3421 tttgacgtat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     3481 gcccctgcag gcagctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc
     3541 gtcgggcgac ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg
     3601 ccaactccat cactaggggt tcctgcggcc gctccccagc atgcctgcta ttctcttccc
     3661 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     3721 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     3781 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     3841 tcgaggctga tcagcgagct ctagttagaa ttcttagctg gcctccacct ttctcttctt
     3901 cttggggctg tcgcctccca gctgagacag gtcgatccgt gtctcgtaca ggccggtgat
     3961 gctctggtgg atcagggtgg cgtccagcac ctctttggtg ctggtgtacc tcttccggtc
     4021 gatggtggtg tcaaagtact tgaaggcggc aggggctccc agattggtca gggtaaacag
     4081 gtggatgata ttctcggcct gctctctgat gggcttatcc cggtgcttgt tgtaggcgga
     4141 cagcactttg tccagattag cgtcggccag gatcactctc ttggagaact cgctgatctg
     4201 ctcgatgatc tcgtccaggt agtgcttgtg ctgttccaca aacagctgtt tctgctcatt
     4261 atcctcgggg gagcccttca gcttctcata gtggctggcc aggtacagga agttcacata
     4321 tttggagggc agggccagtt cgtttccctt ctgcagttcg ccggcagagg ccagcattct
     4381 cttccggccg ttttccagct cgaacaggga gtacttaggc agcttgatga tcaggtcctt
     4441 tttcacttct ttgtagccct tggcttccag aaagtcgatg ggattcttct cgaagctgct
     4501 tctttccatg atggtgatcc ccagcagctc tttcacactc ttcagtttct tggacttgcc
     4561 cttttccact ttggccacca ccagcacaga ataggccacg gtggggctgt cgaagccgcc
     4621 gtacttctta gggtcccagt ccttctttct ggcgatcagc ttatcgctgt tcctcttggg
     4681 caggatagac tctttgctga agccgcctgt ctgcacctcg gtctttttca cgatattcac
     4741 ttggggcatg ctcagcactt tccgcacggt ggcaaaatcc cggcccttat cccacacgat
     4801 ctccccggtt tcgccgtttg tctcgatcag aggccgcttc cggatctcgc cgttggccag
     4861 ggtaatctcg gtcttgaaaa agttcatgat gttgctgtag aagaagtact tggcggtagc
     4921 cttgccgatt tcctgctcgc tcttggcgat catcttccgc acgtcgtaca ccttgtagtc
     4981 gccgtacacg aactcgcttt ccagcttagg gtactttttg atcagggcgg ttcccacgac
     5041 ggcgttcagg taggcgtcgt gggcgtggtg gtagttgttg atctcgcgca ctttgtaaaa
     5101 ctggaaatcc ttccggaaat cggacaccag cttggacttc agggtgatca ctttcacttc
     5161 ccggatcagc ttgtcattct cgtcgtactt agtgttcatc cgggagtcca ggatctgtgc
     5221 cacgtgcttt gtgatctgcc gggtttccac cagctgtctc ttgatgaagc cggccttatc
     5281 cagttcgctc aggccgcctc tctcggcctt ggtcagattg tcgaactttc tctgggtaat
     5341 cagcttggcg ttcagcagct gccgccagta gttcttcatc ttcttcacga cctcttcgga
     5401 gggcacgttg tcgctcttgc cccggttctt gtcgcttctg gtcagcacct tgttgtcgat
     5461 ggagtcgtcc ttcagaaagc tctgaggcac gatatggtcc acatcgtagt cggacagccg
     5521 gttgatgtcc agttcctggt ccacgtacat atcccgccca ttctgcaggt agtacaggta
     5581 cagcttctcg ttctgcagct gggtgttttc cacggggtgt tctttcagga tctggctgcc
     5641 cagctctttg atgccctctt cgatccgctt cattctctcg cggctgttct tctgtccctt
     5701 ctgggtggtc tggttctctc tggccatttc gatcacgatg ttctcgggct tgtgccggcc
     5761 catcactttc acgagctcgt ccaccacctt cactgtctgc aggatgccct tcttaatggc
     5821 ggggctgccg gccagattgg caatgtgctc gtgcaggcta tcgccctggc cggacacctg
     5881 ggctttctgg atgtcctctt taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt
     5941 tctgttggcg aagccgtcgg acttcaggaa atccaggatt gtcttgccgg actgcttgtc
     6001 ccggatgccg ttgatcagct tccggctcag cctgccccag ccggtgtatc tccgccgctt
     6061 cagctgcttc atcactttgt cgtcgaacag gtgggcatag gttttcagcc gttcctcgat
     6121 catctctctg tcctcaaaca gtgtcagggt cagcacgata tcttccagaa tgtcctcgtt
     6181 ttcctcattg tccaggaagt ccttgtcctt gataattttc agcagatcgt ggtatgtgcc
     6241 cagggaggcg ttgaaccgat cttccacgcc ggagatttcc acggagtcga agcactcgat
     6301 tttcttgaag tagtcctctt tcagctgctt cacggtcact ttccggttgg tcttgaacag
     6361 caggtccacg atggcctttt tctgctcgcc gctcaggaag gcgggctttc tcattccctc
     6421 ggtcacgtat ttcactttgg tcagctcgtt atacacggtg aagtactcgt acagcaggct
     6481 gtgcttgggc agcaccttct cgttgggcag gttcttatcg aagttggtca tccgctcgat
     6541 gaagctctgg gcggaagcgc ccttgtccac cacttcctcg aagttccagg gggtgatggt
     6601 ttcctcgctc tttctggtca tccaggcgaa tctgctgttt cccctggcca gagggcccac
     6661 gtagtagggg atgcggaagg tcaggatctt ctcgatcttt tcccggttgt ccttcaggaa
     6721 tgggtaaaaa tcttcctgcc gccgcagaat ggcgtgcagc tctcccaggt ggatctggtg
     6781 ggggatgctg ccgttgtcga aggtccgctg cttccgcagc aggtcctctc tgttcagctt
     6841 cacgagcagt tcctcggtgc cgtccatctt ttccaggatg ggcttgatga acttgtagaa
     6901 ctcttcctgg ctggctccgc cgtcaatgta gccggcgtag ccgttcttgc tctggtcgaa
     6961 gaaaatctct ttgtacttct caggcagctg ctgccgcacg agagctttca gcagggtcag
     7021 gtcctggtgg tgctcgtcgt atctcttgat catagaggcg ctcagggggg ccttggtgat
     7081 ctcggtgttc actctcagga tgtcgctcag caggatggcg tcggacaggt tcttggcggc
     7141 cagaaacagg tcggcgtact ggtcgccgat ctgggccagc aggttgtcca ggtcgtcgtc
     7201 gtaggtgtcc ttgctcagct gcagtttggc atcctcggcc aggtcgaagt tgctcttgaa
     7261 gttgggggtc aggcccaggc tcagggcaat caggtttccg aacaggccat tcttcttctc
     7321 gccgggcagc tgggcgatca gattttccag ccgtctgctc ttgctcagtc tggcagacag
     7381 gatggccttg gcgtccacgc cgctggcgtt gatggggttt tcctcgaaca gctggttgta
     7441 ggtctgcacc agctggatga acagcttgtc cacgtcgctg ttgtcggggt tcaggtcgcc
     7501 ctcgatcagg aagtggcccc ggaacttgat catgtgggcc agggccagat agatcagccg
     7561 caggtcggcc ttgtcggtgc tgtccaccag tttctttctc aggtggtaga tggtggggta
     7621 cttctcgtgg taggccacct cgtccacgat gttgccgaag atggggtgcc gctcgtgctt
     7681 cttatcctct tccaccagga aggactcttc cagtctgtgg aagaagctgt cgtccacctt
     7741 ggccatctcg ttgctgaaga tctcttgcag atagcagatc cggttcttcc gtctggtgta
     7801 tcttcttctg gcggttctct tcagccgggt ggcctcggct gtttcgccgc tgtcgaacag
     7861 cagggctccg atcaggttct tcttgatgct gtgccggtcg gtgttgccca gcaccttgaa
     7921 tttcttgctg ggcaccttgt actcgtcggt gatcacggcc cagcccacag agttggtgcc
     7981 gatgtccagg ccgatgctgt acttcttgtc ggacgcttcg accttgcgct ttttcttcgg
     8041 cgaagcgtaa tctggaacat cgtatgggta catggtggca ccggtccaac ctgaaaaaaa
     8101 gtgatttcag gcaggtgctc caggtaatta aacattaata ccccaccaac caaccatccc
     8161 ttaaaccctt acctcttgct cagctaatta cagcccggag gagaagggcc gtcccgcccg
     8221 ctcacctgtg ggagtaacgc ggtcagtcag agccggggcg ggcggcgcga ggcggcggcg
     8281 gagcggggca cggggcgaag gcagcgcgca gcgactcccg cccgccgcgc gcttcgcttt
     8341 ttatagggcc gccgccgccg ccgcctcgcc ataaaaggaa actttcggag cgcgccgctc
     8401 tgattggctg ccgccgcacc tctccgcctc gccccgcccc gcccctcgcc ccgccccgcc
     8461 ccgc
//
