LOCUS       sequence-363057-       12057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pAIDE containing a full-length CPSMV RNA2 cDNA modified to 
            accomodate an NbPDS fragment..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12057)
  AUTHORS   Zaulda FA, Yang SH, Han J, Mlotshwa S, Dorrance A, Qu F
  TITLE     A cowpea severe mosaic virus-based vector simplifies virus-induced 
            gene silencing and foreign protein expression in soybean.
  JOURNAL   Plant Methods. 2022 Oct 28;18(1):116. doi: 
            10.1186/s13007-022-00950-7.
  PUBMED    36307846
REFERENCE   2  (bases 1 to 12057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2022-10-28"; volume: "18(1):116. doi"; pages: " 
            10.1186/s13007-022-00950-7"
COMMENT     Sequence Label: FZ-NbPDS
FEATURES             Location/Qualifiers
     source          1..12057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4434..4450)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4434..4450)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4447..4469)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4458..4474
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4482..4488,4489..4506,4507..4512))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4527..4548
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4752..4776
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5201..5995
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6082..6670
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6571..6590
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6824..6841
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6856..6996
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6922..6941)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7082..7104
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7340..7534
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7600..8673)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9102..9731)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11032..11056
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     11244..11266
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11258..11275
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11259..11275
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11708..12052
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     11996..12016
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tattaaaatt tttctaggaa aattttaata caaaagcaat cagaggtttg tgccacctta
       61 aaacgcacac aagctttcgt accctctttt caagagtcaa tccgtactcg agataccttg
      121 aaagagatac attcttcctc tcttactttt ctttatttct ctttaaaact ctcttctttc
      181 tgattatcag attccaacaa tctcaggctt aacagagata ctggggacat accaagctgc
      241 attaaaatat tgatatgtca actttccgtt ataagtgcaa gcagttggat caagagatac
      301 agtggtggtt ttctggaaca ggcaaccgag cattttggaa atttgagaaa aaattggctg
      361 aattgcacga gtggtactgg agtttggcac tagacccatt tccctattct gggttctttt
      421 acaagtgctt ttacgaatta tttcaactgt gggtcaaact aggactaatt gtgcaagtaa
      481 gctaccttat tttgctcctt gactttttcg tctacaccat tccaaagaaa atggctagtc
      541 agatagaaac tacagtcgaa aaggttaaac aaagtggaat accagcagac atactgcgta
      601 aacgcgctgt ggattattgg aagaaaaaca atagccacaa ctcgcagatg caagatgtcc
      661 tacccaatgt ggatgaaatc tacgagggta tgcgagcaaa tattgcaaaa tatcttggtc
      721 gcagttccac tgtcacaagt attgccaagc tcggaaagtg caaggtgtat cgaaagaaaa
      781 atataccatt agccaacttg ccaagtttgc aaaccagctg tgtgcccatc acactgactg
      841 aggaaagtgt aggaaattct gactacacaa ctgaagaaac caattcagaa gtgaaatcat
      901 tacacgttgg agctattgaa atcgtgatga atagctttgc gagtagtgat tgtaacattc
      961 ttggtggttt cttactaatt gatacatgcc atacagatat caacaatgct attcgtagta
     1021 tttttgtggc accaatgaga ggaggtcgac caattaggat gatttctttt cccgacactc
     1081 tggtccagat tgagcctaac atgaataagc gttttcaatt gctgtgtaca acaagcaatg
     1141 gagacttcat gcaagggcga gatctggcga tgatgcatgt gaatgtgcta gctcatgctg
     1201 tcacgcacac atccacctat actcctacac cctactatga gaaaattctc tcacgagaaa
     1261 aaggatttat tgtggagtat ctgaatagga tgacttatgc ggttcataac caaaatcatc
     1321 ctactgaaaa agatttgctt gaaagtgatt tccaatttga ctttgaaggg caaccagtgc
     1381 ttaagagaat ctcatctacc aaagctattt tctctaaagg ttctagtttt cgatatatga
     1441 tttctggcaa aaaagagcac aagattgaca agccaaggct agaagaagat ggtagtaaga
     1501 gttacattga tggtttacaa gatacctttg acacgactca tgctactctg caatcaggtg
     1561 cagacctgtt taagaggaac ctagacgatg tctcgactat ctcagatacg atgcacgtgc
     1621 aaggtgtgcc tgatagggtg acagatgagg tgttcattgc catgtcaaag gcacttaact
     1681 tcataaaccc tgacgagctt tcgatgcagt gcattttgat tgctttgaac agatttcttc
     1741 aggagaaaca tggttcaaaa atggcctttt tagatggtaa ccctcctgag agactttgca
     1801 tgccgattgt ggaacatatt gagtcaaaag gtggccaagt cagactaaac tcacgaataa
     1861 aaaagatcga gctgaatgag gatggaagtg tcaaatgttt tatactgaat aatggcagtc
     1921 acgtgacgtt cgataccaca cacgcaaccc tacagtctgg ggctgatcta ttcaaacgaa
     1981 atttggatga tgtaagcacc atttcggaca ccatgcttgg ggccatgatt ggacaaacca
     2041 aggtggtgat tccaaaaaca ttagttgcag gtacagttct caaaagtgga ccgctttcag
     2101 atgtgatgca gcagggatca ttccgatcaa caatagcatt gcaaagaaca catataataa
     2161 ctggaaaaat acatgttgtt gcgatgcttg aaactgctgt aaatacagga ctgggattgg
     2221 ccatttgttt caatagtggc attcgaggaa aagcttctgc agacatctat gcaacgtgtt
     2281 cacaagatgc catgatctgg aatccatcat gcaccaaggt tatgcaatat gcatttaacc
     2341 ctaacccgtg ttctgatggc tggagcttgg cttttttgga gagaactggt taccattgtg
     2401 tggtcacatg cgtgacagga tggactggaa cgccattgca agacaccttt atgaccataa
     2461 actggcatat ttctagggaa gcatgtgtac caaaaatata taccattttt gatccagaac
     2521 cagatatgat gttaaataga tggatgggac gtgctatctt tcctcagcaa agtacgcagg
     2581 ttgtgaggag aatgccactt tccattggtg gtggtgcagg tgcaaagaat tcaattttga
     2641 tgaaccttcc aaatgcaata ctatcaatgt ggcgttactt caaggctgat cttgagtttg
     2701 aactcatcaa gatgtcctcc ccatatataa atgcgacaat agcatttttt gtggcctttg
     2761 gggatctctc tgatgataca gttaattttg aagcttttcc ccacaagtta attgtctttt
     2821 ctgacaagca agacaggacg accatatctt tctctaagga tgaatttttg atggcatggt
     2881 ccacacaggt taggcccgat actaaactca gtgaggatgg ttgcccctac ctgtatgcca
     2941 taacgcacaa tggtgttagt tcttctgtgg aaggagattt tattcttggt atcaagatgg
     3001 tcgggctcaa agctgtagaa aacataggag tgaatccagg aatcattgga tcgcgcttgt
     3061 taggggcagt tgcccaatct ggacagacac agcaggtttg gaataagatc tggcgcattg
     3121 gaactccacc gcaagccaca gatggtttgt tctcattttc tatagatctt cttggagttg
     3181 aactggttac tgatggccag gagggggcag tttctgtatt gagcagcagt ccagtagcaa
     3241 atttgctacg cacagcggct tggaagtgtg gaacgctgca tgttaaagtt gttatgactg
     3301 ggagagttac taccactcga gccaattggg catcacatac ccaaatgtca ctagtgaatt
     3361 ctgacaatgc tcagcattat gaagcacaga aatggagtgt ttctacacca catgcgtggg
     3421 aaaaggaatt ttctatagac atatgtggtc ccaacagggg ttttgagatg tggcgctcat
     3481 cttggagtaa tcaaaccaca tggatattag aatttacggt tgctggagct tctcagtccg
     3541 caatattcga aatattctat cggcttgata attcatggaa aagtgctgga aatgttttaa
     3601 tgccaccatt gttggttggg aatcccaggc ttgacatcaa aggtcgtgcc gctgcagctg
     3661 catagtacta tactttaact atttgcttta aataagcctc ataagtctat gaccccaatt
     3721 tggtatgctc tagcagaggt aagtttggtt tgcaatttct ttatttttgt tgtaaacatt
     3781 gtaattagcg tattgtgtgg tagtatatgt tatgctcttt tcacatttca tccatgtatt
     3841 cccaagtcac tttgactcta ggtaagtgga ttaagcctta cataccaggc cggtcttggg
     3901 cagcgactgt taaatatgtc caatcactga ggtatcatac gccatttagt tggttgtgtt
     3961 gatatcgtct aaacagttgg cgttaacgct tatgcataca ttcagatttt atgtgcttta
     4021 ttgcatgtac gaatgtacag catttgctgt tgtaacgggc tagtttagaa ttatttataa
     4081 aagtgtgcaa tgtagtaatt atgtgtgact ttaattgttc attgttcttc ttttaaaaaa
     4141 aaaaaaaaaa aaaaaaaaac tagtccgcaa aaatcaccag tctctctcta caaatctatc
     4201 tctctctatt tttctccaga ataatgtgtg agtagttccc agataaggga attagggttc
     4261 ttatagggtt tcgctcatgt gttgagcata taagaaaccc ttagtatgta tttgtatttg
     4321 taaaatactt ctatcaataa aatttctaat tcctaaaacc aaaatccagt gacctgcaga
     4381 aaggcgcgcc aaagaattct ttggctgacc atgattacga attcgtaatc atggtcatag
     4441 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     4501 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     4561 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     4621 cgcgcgggga gaggcggttt gcgtattggc tagagcagct tgccaatcca gatcccccga
     4681 attaattcgg cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta
     4741 agcgtcaatt tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac
     4801 cggcagctcg gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc
     4861 agcgggagag ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga
     4921 acggcaacta agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat
     4981 tgtaacgatg acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg
     5041 atactatgtt atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa
     5101 ggttttagaa tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg
     5161 gtatctttaa atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc
     5221 accggaattg aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc
     5281 tcctgctaag gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga
     5341 cagccggtat aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct
     5401 ggaaggaaag ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa
     5461 tctgctcatg agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag
     5521 ccctgaaaag attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat
     5581 atcggattgt ccctatacga atagcttaga cagccgctta gccgaattgg attacttact
     5641 gaataacgat ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga
     5701 tccgcgcgag ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc
     5761 ccacggcgac ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat
     5821 tgatcttggg agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc
     5881 gatcagggag gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat
     5941 caagcctgat tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct
     6001 agaatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6061 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6121 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6181 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6241 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6301 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6361 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6421 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6481 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6541 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6601 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6661 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6721 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6781 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     6841 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     6901 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     6961 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     7021 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7081 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt
     7141 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag
     7201 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga
     7261 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc
     7321 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt
     7381 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg
     7441 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta
     7501 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc
     7561 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc
     7621 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc
     7681 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa
     7741 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc
     7801 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc
     7861 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc
     7921 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt
     7981 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt
     8041 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc
     8101 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc
     8161 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat
     8221 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat
     8281 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc
     8341 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact
     8401 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt
     8461 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat
     8521 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg
     8581 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat
     8641 ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca
     8701 gtgccattgc agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct
     8761 ccacacatgg ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct
     8821 cctttagccg ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg
     8881 cgcgatgtat tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt
     8941 cagcttggtg tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc
     9001 tgccaggctg gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag
     9061 cgtgtttgtg cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta
     9121 atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga
     9181 acggttgtgc cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca
     9241 agaatgggca gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct
     9301 ttgatcgccc gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc
     9361 tgcttaacca gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc
     9421 ggaatcagca cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc
     9481 gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc
     9541 gggcggtcga tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg
     9601 gcactgccct ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg
     9661 cgggctagat gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg
     9721 ataaccttca tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag
     9781 cgaccgcatg acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct
     9841 cggtttcttc agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg
     9901 ccaggatttc atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg
     9961 ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct
    10021 ggtgcggttt catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc
    10081 ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc
    10141 ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc
    10201 ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg
    10261 ggtgagggta gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct
    10321 ccgggtgcgg tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa
    10381 caccatgcgg ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc
    10441 cgcgccggcc tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag
    10501 gcggtctagc ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc
    10561 cagctccggg cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc
    10621 ggccgcgtgc agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc
    10681 atagcccagc aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc
    10741 gcaagtattc tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag
    10801 ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg
    10861 cactgaacgt cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg
    10921 atcccgaggg gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt
    10981 tcacgccctt ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg
    11041 ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc
    11101 caagctcaag ctgctctagc attcgccatt caggctgcgc aactgttggg aagggcgatc
    11161 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    11221 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa
    11281 gcttgttagg cctaaacctg caggtcaaca tggtggagca cgacacactt gtctactcca
    11341 aaaatatcaa agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg
    11401 taatatccgg aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga
    11461 tagtggaaaa ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg
    11521 ttgaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
    11581 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gataacatgg
    11641 tggagcacga cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa
    11701 gggcaattga gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc
    11761 cagctatctg tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc
    11821 atcattgcga taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag
    11881 atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa
    11941 agcaagtgga ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc
    12001 cttcgcaaga cccttcctct atataaggaa gttcatttca tttggagagg accggtt
//
