LOCUS       sequence-36307-e        8719 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLP recombinase in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Nern A, Pfeiffer BD, Svoboda K, Rubin GM
  TITLE     Multiple new site-specific recombinases for use in manipulating 
            animal genomes.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Aug 9.
  PUBMED    21831835
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2011 Aug 9."
COMMENT     Sequence Label: pBPhsFlp1
FEATURES             Location/Qualifiers
     source          1..8719
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        301..539
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     486..507
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             595..1866
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     CDS             complement(join(3242..3856,3927..4058,4262..4577,4639..5293,
                     5368..5641,6043..6114))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     6566..6584
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    6809..6878
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(6965..7553)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7045..7064)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7724..8515,8516..8584))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8347..8366
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8585..8689)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcggccg gccagatctg aatcccaaaa caaactggtt attgtggtag
      121 gtcatttgtt tggcagaaag aaaactcgag aaatttctct ggccgttatt cgttattgtc
      181 tcttttcttt ttgggtctct ccctctctgc actaatgctc tctcactctg tcacacagta
      241 aacgacatac tgctctcgtt ggttcgagag agcgcgcctc gaatgttcgc gaaaagagcg
      301 ccggagtata aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag
      361 tgaacacgtc gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca
      421 atctgcagta aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat
      481 caactgcaac tactgaaatc tgccaagaag taattattga atacaagaag agaactctga
      541 atactttcaa caagttaccg agaaagaaga actcaggtac cgctagcaat caaaatgcca
      601 caatttgata tattatgtaa aacaccacct aaggtgcttg ttcgtcagtt tgtggaaagg
      661 tttgaaagac cttcaggtga gaaaatagca ttatgtgctg ctgaactaac ctatttatgt
      721 tggatgatta cacataacgg aacagcaatc aagagagcca cattcatgag ctataatact
      781 atcataagca attcgctgag tttcgatatt gtcaataaat cactccagtt taaatacaag
      841 acgcaaaaag caacaattct ggaagcctca ttaaagaaat tgattcctgc ttgggaattt
      901 acaattattc cttactatgg acaaaaacat caatctgata tcactgatat tgtaagtagt
      961 ttgcaattac agttcgaatc atcggaagaa gcagataagg gaaatagcca cagtaaaaaa
     1021 atgcttaaag cacttctaag tgagggtgaa agcatctggg agatcactga gaaaatacta
     1081 aattcgtttg agtatacttc gagatttaca aaaacaaaaa ctttatacca attcctcttc
     1141 ctagctactt tcatcaattg tggaagattc agcgatatta agaacgttga tccgaaatca
     1201 tttaaattag tccaaaataa gtatctggga gtaataatcc agtgtttagt gacagagaca
     1261 aagacaagcg ttagtaggca catatacttc tttagcgcaa ggggtaggat cgatccactt
     1321 gtatatttgg atgaattttt gaggaattct gaaccagtcc taaaacgagt aaataggacc
     1381 ggcaattctt caagcaataa acaggaatac caattattaa aagataactt agtcagatcg
     1441 tacaataaag ctttgaagaa aaatgcgcct tattcaatct ttgctataaa aaatggccca
     1501 aaatctcaca ttggaagaca tttgatgacc tcatttcttt caatgaaggg cctaacggag
     1561 ttgactaatg ttgtgggaaa ttggagcgat aagcgtgctt ctgccgtggc caggacaacg
     1621 tatactcatc agataacagc aatacctgat cactacttcg cactagtttc tcggtactat
     1681 gcatatgatc caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag
     1741 gagtggcagc atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca
     1801 tggaatggga taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc
     1861 atataagttt aaaccctagg gtcgactaaa gccaaataga aaattattca gttcctggct
     1921 taagttttta aaagtgatat tatttatttg gttgtaacca accaaaagaa tgtaaataac
     1981 taatacataa ttatgttagt tttaagttag caacaaattg attttagcta tattagctac
     2041 ttggttaata aatagaatat atttatttaa agataattgc gtttttattg tcagggagtg
     2101 agtttgctta aaaactcgtt tagatctgtc ctcgagaaat tatttattta aatgcgatgg
     2161 agagttggcg ccgaatcgaa aactttacgc gcttaaaagc acgagttggc atccctaacc
     2221 ggtgtttaaa cgcggccgct ggccacgggt gcgcatgatc gtgctcctgt cgttgaggac
     2281 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
     2341 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
     2401 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgccctgca ccattatgtt
     2461 ccggaggcgc gccctagttc cagtgaaatc caagcatttt ctaaattaaa tgtattctta
     2521 ttattatagt tgttattttt gatatatata aacaacacta ttatgcccac catttttttg
     2581 agatgcatct acacaaggaa caaacactgg atgtcacttt cagttcaaat tgtaacgcta
     2641 atcactccga acaggtcaca aaaaattacc ttaaaaagtc ataatattaa attagaataa
     2701 atatagctgt gagggaaata tatacaaata tattggagca aataaattgt acatacaaat
     2761 atttattact aatttctatt gagacgaaat gaaccactcg gaaccatttg agcgaaccga
     2821 atcgcgcgga actaacgaca gtcgctccaa ggtcgtcgaa caaaaggtga atgtgttgcg
     2881 gagagcgggt gggagacagc gaaagagcaa ctacgaaacg tggtgtggtg gaggtgaatt
     2941 atgaagaggg cgcgcgattt gaaaagtatg tatataaaaa atatatcccg gtgttttatg
     3001 tagcgataaa cgagtttttg atgtaaggta tgcaggtgtg taagtctttt ggttagaaga
     3061 caaatccaaa gtctacttgt ggggatgttc gaaggggaaa tacttgtatt ctataggtca
     3121 tatcttgttt ttattggcac aaatataatt acattagctt tttgaggggg caataaacag
     3181 taaacacgat ggtaataatg gtaaaaaaaa aaaacaagca gttatttcgg atatatgtcg
     3241 gctactcctt gcgtcgggcc cgaagtctta gagccagata tgcgagcacc cggaagctca
     3301 cgatgagaat ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg
     3361 tctccaggat gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt
     3421 cgcccggctc cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc
     3481 atgagaggta cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagc
     3541 cgccaaagag caggaatggt atgataaccg gcggacccac agacagcgcc atcgaggtcg
     3601 aggagctggc gcaggatatt agatatccga aggacgttga cacattggcc accagagtga
     3661 ccagcgccag gcagttgaag aagtgcagca ctccggcccg cagtccgatc atcggatagg
     3721 caatcgccgt gaagaccagt ggcactgtga gaaaaagcgg caattcggca atcgttttgc
     3781 ccagaaagta tgtgtcacag cgataaagtc gacttcgggc ctccctcata aaaactggca
     3841 gctctgaggt gaacacctaa atcgaatcga ttcattagaa agttagtaaa ttattgaaat
     3901 gcaaatgtat tctaaacatg acttacattt atcgtggcaa agacgttttg aaaggtcatg
     3961 ttggtcagga agaggaagat ggctccgttg atattcatca cacccacttg cgtgagttgt
     4021 tggcccaaaa agatgaggcc aatcaagatg gcaaccatct gcaaattaaa atgttactcg
     4081 catctcatta atattcgcga gttaaatgaa atttatttat cttctgcaaa actataaact
     4141 atacatctca ttgaaaaaaa ctaagaaggg tgtggaatca ggcaattcta tctaaaatct
     4201 agcgaatttg tttccaagaa ttgtaagcgt tatatcattt gtttccactg gaaccactca
     4261 ccgttgtctg aataagtcgc acttttacga ggagtggttc cttgagcacc gacagccagg
     4321 atcgccacag gaccgcccgg aactgcatga accaggtggc cttgtaggtg tacccattct
     4381 ccggctgctc cagtggcttc tccagatttt tggtggccaa caactgctcc atatcccggg
     4441 ctactttgct aatggcaaaa ttgtcgcata tcttggcgat ccgatcacgg gactcgatct
     4501 cccgtccggg cacaacggcc aacacctgta cgtaaaagtc cgccggattg tagttggtag
     4561 gacactgggc acccacgctg gataggagtt gagatgttat gtaatactag atacccttaa
     4621 taaacacatc gaactcacta ggaaaagaag tcgacggctt cgctgggagt gcccaagaaa
     4681 gctaccctgc cctcggccat cagaaggatc ttgtcaaaga gctcaaacag ctcggaagac
     4741 ggctgatgaa tggtcaggat gacggtcttg cccttctgcg acagcttctt cagcacctgg
     4801 acgacgctgt gggcggtaaa ggagtccagt ccggaggtgg gctcatcgca gatcagaagc
     4861 ggcggatcgg ttagagcctc ggaggcgaat gccagacgct tcctttctcc gccggacaga
     4921 cctttcaccc tgccgggcac accgatgatc gtgtgctgac atttgctgag cgaaagctcc
     4981 tggatcacct gatccacgcg ggccactcgc tgccgatagg tcagatgtcg tggcatccgc
     5041 accatggctt ggaaaatcag gtgttccctg gccgttaggg agccgataaa gaggtcatcc
     5101 tgctggacat aggcgcacct ggcctgcatc tccttggcgt ccacaggttg gccattgagc
     5161 agtcgcatcc cggatggcga tacttggatg ccctgcggcg atcgaaaggc aagggcattc
     5221 agcagggtcg tctttccggc accggaactg cccatcacgg ccaaaagttc gcccggatag
     5281 gccacgccgc aaactgagtt tcaaattggt aattggaccc tttattaaga tttcacacag
     5341 atcagccgac tgcgaataga aactcaccgt tcttgagcaa atgtttcctg ggcgccggta
     5401 tgtgtcgctc gttgcagaat agtccgcgtg tccggttgac cagctgccgc catccggagc
     5461 ccggctgatt gaccgcccca aagatgtcca tattgtgcca ggcataggtg aggttctcgg
     5521 ctagttggcc gctccctgaa ccggagtcct ccggcggact gggtggccgg agcgtgccgt
     5581 agtttttggc ctgcccgaag ccctggttaa tgcagctctg cgaagccgct ccgctgtcac
     5641 cctgcaatga taggggatct caaatatcaa ctactagcgt tatgctcatc taaccccgaa
     5701 caaaaagtac cccgaagtat cctacgaagt aggtttatac ttttatttat tttttgtgca
     5761 tctaggatca gcttaaaata tctggttgtt atattttttg taaaaaagaa tatagtcgaa
     5821 aatgaatgcc tttagatgtc ttgatcatga tatgatctca aaaattgtct tatatagcga
     5881 gaacagctac cagaataatc tgtttcgtgt cactatttgt ttgtgcaatt gcggtttggg
     5941 atttttgtgg gtcgcagttc tcacgccgca tacaatttga tgttgcaatc gcagttccta
     6001 tagatcaagt gaacttaaga tgtatgcaca tgtactactc acattgttca gatgctcggc
     6061 agatgggtgt ttgctgcctc cgcgaattaa tagctcctga tcctcttggc ccattgccgg
     6121 gatttttcac actttcccct gcttacccac ccaaaaccaa tcaccacccc aatcactcaa
     6181 aaaacaaaca aaaataagaa gcgagaggag ttttggcaca gcactttgtg tttaattgat
     6241 ggcgtaaacc gcttggagct tcgtcacgaa accgctgaca aaatgcaact gaaggcggac
     6301 attgacgcta cgtaacgcta caaacggtgg cgaaagagat agcggacgca gcggcgaaag
     6361 agacggcgat atttctgtgg acagagaagg aggcaaacag cgctgacttt gagtggaatg
     6421 tcattttgag tgagaggtaa tcgaaagaac ctggtacatc aaataccctt ggatcgaagt
     6481 aaatttaaaa ctgatcagat aagttcaatg atatccagtg cagtaaaaaa aaaaaatgtt
     6541 ttttttatct actttccgca aaaatgggtt ttattaactt acatacatac taggcgcgcc
     6601 catatgttcg gcttgtcgac atgcccgccg tgaccgtcga gaacccgctg acgctgcccc
     6661 gcgtatccgc acccgccgac gccgtcgcac gtcccgtgct caccgtgacc accgcgccca
     6721 gcggtttcga gggcgagggc ttcccggtgc gccgcgcgtt cgccgggatc aactaccgcc
     6781 acctcgaccc gttcatcatg atggaccaga tgggtgaggt ggagtacgcg cccggggagc
     6841 ccaagggcac gccctggcac ccgcaccgcg gcttcgagac cgtgacctac atcgtcgacg
     6901 gtaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6961 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7021 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     7081 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     7141 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     7201 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     7261 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     7321 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     7381 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     7441 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7501 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7561 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7621 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7681 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7741 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7801 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7861 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7921 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7981 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     8041 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     8101 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     8161 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     8221 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     8281 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     8341 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     8401 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     8461 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8521 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8581 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     8641 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8701 gaaaagtgcc acctgagtc
//
