LOCUS       sequence-363074-       14273 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A binary plasmid derived from pCambia1300, with an insert that is a 
            full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and 
            terminator..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14273)
  AUTHORS   Zaulda FA, Yang SH, Han J, Mlotshwa S, Dorrance A, Qu F
  TITLE     A cowpea severe mosaic virus-based vector simplifies virus-induced 
            gene silencing and foreign protein expression in soybean.
  JOURNAL   Plant Methods. 2022 Oct 28;18(1):116. doi: 
            10.1186/s13007-022-00950-7.
  PUBMED    36307846
REFERENCE   2  (bases 1 to 14273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2022-10-28"; volume: "18(1):116. doi"; pages: " 
            10.1186/s13007-022-00950-7"
COMMENT     Sequence Label: QUIN
FEATURES             Location/Qualifiers
     source          1..14273
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(6650..6666)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6650..6666)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6663..6685)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6674..6690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6698..6704,6705..6722,6723..6728))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6743..6764
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    6968..6992
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7417..8211
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      8298..8886
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8787..8806
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9040..9057
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9072..9212
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9138..9157)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9298..9320
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9556..9750
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9816..10889)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11318..11947)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13248..13272
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     13460..13482
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13474..13491
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13475..13491
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        13924..14268
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     14212..14232
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 tattaaaatt ttcaagagaa gattttgata ccaaaagcga tcagaggttt gtgccacctt
       61 aaaacgcaca acaaaaagct ttcgtaccct cttttcaaga gtcaatccgc attcgagata
      121 ccttgaaaga gtacttttct ttccctcttt tcttaattgc tacttacgtt ttcaaaatca
      181 ctttttctct cttaagctac acaagtttaa ctaaaagcct agaacgaact ttttattgga
      241 cgcattaaac tacaatatga agttctttgc tgggcaaact gttatgaatg tgctgcaaca
      301 tgtttcctct cccaccacca atttaaggtt gttatcttat tgcaatctta aaaaggaaga
      361 ggatggtaag atgatgctgg ctattaagga gcagaggcac cgacggttgt tgacactgtc
      421 atatggtgct atgtgttttc aattctcaaa ttcagtaggg gatgagggca tagaagtgga
      481 tgatgatgag ttgatgtttg aaatatttga tgcattgctg cgcacaaaga tttcaaactc
      541 aaagggcatg acgcacttat acagctggat gcgtggagtt tatctcagca cattcaaagt
      601 agaggtgcag tgtgatgatt acaactcaaa tctgctggag aaggatttgg ctggagaagc
      661 tcagggtctt tcacagttcg tttcaggact tgctgactgg attcccagtc gtgtaaagac
      721 cttggcagga tatgccgctg agggcataat tgaggccttt aagaagcact tcgacaaatt
      781 gcttgttgag tactgtccta tggccgtggc tgcgtgtagt tggataacta cagtatggac
      841 cactatcaaa gagtgggtcc aatctgcgat ggatgctatg tcgtggatta tggctggatg
      901 cactgaactc atttcttggg gaatgtgtgt cattgcaggt tcatgtgctt tatcgttatt
      961 ggaaaaggca ttggtagcta tgggtttgat ttcttcttcc tttgatttag ctggtatctt
     1021 tgtacgctct gcggttgttg gagccttttg tttgacggtt gtcaacaaga ggagccgcaa
     1081 ttgcgctgag ttgttacaac tagtttcatt ggcggtggga gcagtttcaa gtgcaacatc
     1141 atcttgtttc cagtctcctg tagggcaggc aacagatgtg agcgctgaga gtcaatcagg
     1201 tggagttgag atgctagagt cacttgctaa gaacttgacc aatttctgtg atggcactct
     1261 ggtatctatc ggaaagactt gcaacgctgt aaattcgatc aatacggctg caggtaccat
     1321 caaaaatctg gtaggtagac tcttgtctat gctaagtaat tttgcataca aactactagg
     1381 tttagaatcc acattcttgc gagacgcttc cgtggttttt tctgaaaacg ttgatgggtg
     1441 gctaaagcaa atatcttggt gccaagatca atttcttgcc aaggcataca tcaaccagga
     1501 tgaacttatg gtgttgcgat ctctcattac cagaggtgaa gttatgcaaa gggagatgat
     1561 catgggtggt atgaaagttt cacctacagt ttgtggtttg atcaataaag gatgcacgga
     1621 tctcgccaaa ttaatggcag gggctgtgat gcatggaacg agtggtactc ggaaaatacc
     1681 atttgttgtt tatgcacatg gggcttctag agttggtaaa acaatggtga tcaacagact
     1741 cattgaagat tttcgcaaag agttggaact tggagaggac tgtgtgtatc cacgaaatgt
     1801 ggtagatgac tactggagtg ggtacaaaag acaacctatt gttgtcattg atgattttgg
     1861 tgctgtgtct tcagatcctt ctgcagaagc tcaattaatt ccattgatct ctagtgctcc
     1921 ctatcccctt aacatggctg atctctctga gaagggaatg cactttgatt cagctatcgt
     1981 catgtgctca tccaatttca ttgagtgttc accagaaagc aaggtacttt ctcaaaatca
     2041 aaagaaactg tagtgtaaca aatttcttca cttgatgtga tgtatatata tctttagcta
     2101 tctagtgttc atatatgtaa ctcgttgttc aagaacttct ttgtgtaatt catccgcagg
     2161 tgcgtgacga aatggcattc agaaacagac gacatgtgct cttcactgtc tcacttgacc
     2221 ctaatatacc atatgatggt gatgatatca caaagaatca aatatatgaa atcaaaactt
     2281 ggtttcatga ttcgtatcat gttgaagcaa ctttcacatc atatggggac ttgctggcat
     2341 attgcaaaaa caagtgggtg gagcacaata ctgagcaaga ggccaacttg aagcaacttg
     2401 gagttaaaaa ggagagcgtt gcatttcagc agtttcgttc cattcttgat ttggcagtct
     2461 ttgtcaatca agatgcggag aatttcaagc aaaggctgga gacgccagat ggtaggtgcc
     2521 actttgtgtc atgttatgat aagagtggta tactcaggca ctatactatt gatgcaactg
     2581 gagatgtgca agaaatggaa aaggttgatt cctctctaga tgacatccta ttggaaaaaa
     2641 ccaacaaaat ggtcttagct gcatataaga tgattaagta ccacaaggac accaatctgg
     2701 tcatcaaaac ccagcttgca gatttggtgg atcccacaaa gtatactgca gatttccagt
     2761 ttgacggtgt tataggatca ccacttttca gcagccaagt aatgccaagt gtcaaggcat
     2821 taccactgtg gcaaaggatg gtactgtaca ctgttgggca gaatctggga agaactcatt
     2881 ctagttggta tgagggcatc aaggacaagt gtatgcttgc actatcaaaa gcatactcaa
     2941 ctgagatcaa ggattggcct gtagcactca aaattgttgt tggagtgata ctggctactg
     3001 tagcaggttt actaccatgc tgtcttgtgg gtttaacaat tcatctggta tcctgcttat
     3061 taatatgttg tttatgtttt tcttgtaggt aaggcatttt ggaggttcta tgcctcaatg
     3121 gcagatgcag gcaatggtgg acactttgtg ggagccgttg cttccgcatt tgcaggaagt
     3181 caagcggttg ttgcacagag taggaagccc aacaggtttg atgtggctca gtacagatac
     3241 cgaaacatac ctctaaggaa gagaaattgg gcagaagggc aaatgagtct ggatcagtcc
     3301 acaatgctca taatggaaaa gtgcaaggcc aatttcgtct ttagcaacat tagctgtcag
     3361 atagttatgt tgcctgggcg acaattcttg tgctacaaac atgtgtttgc tagtctcaat
     3421 agtccaatgt atgtggatat ttatactgcc aacaagaagt ataaactcta ttacaaacct
     3481 cagaataggg tatactttga gactgatagt gagatcatgc tatacaagga tgccagtttg
     3541 gaagacatac ctgccagctg ctgggatctt ttttgttttg atgcggaaaa aagtctgcca
     3601 cgaggtatgt ggatgagttc ttgtgtattt tatcaggaga ttgtaaattt agtgcaatct
     3661 tgaattatcg attctattac tccacaggta gtttcccagc agaaatcctc tcgtgcaaac
     3721 tagatcggac aacgaatcaa catatcccgg agtgggccga catctcagct cgtactgtca
     3781 atcaaaaact ggacgtggaa tttggggagt accaaaccat cttttattcc tatctccagt
     3841 atgatgtacc cacaaaagct gaagattgtg gttccctaat aatagcaacc attgatggta
     3901 ggaaaaagat aataggcatc cacactgctg gacgggcaaa taggagtggt tttgcaagtt
     3961 atatgccgca ggtagaaata ccagttcaag cacaagcagc ggaaaagttc tttgattttc
     4021 ttgagaaaga acaacatgtt actgagggca ttggaaaggt gggaaatctc aagaaaggag
     4081 tctgggttcc attacccact aagaccaatc ttgtggaaac accaaaagag tggcatctgg
     4141 gcactgagaa aacaaaagaa ccaagtattc tcagcagtac ggatttaagg ctcggtgata
     4201 agcagtatga tccctttgtt ggaggaatac agaagtacgc cgaaccaatg ggaatactag
     4261 atgatgaggt gctccggcat gtggcaacag acatagttga agaatggttt gactgtgtag
     4321 accctcaaga agatactttt gaggaagttg acctacaggt tgctatcaat ggtcttgaag
     4381 gaatggaata catggaaaga gttcctatgg caacatctga aggcttccca cacattttga
     4441 caaggaaaag tggggaaaaa ggcaaaggtt agtgaagttg taccatttat ttagcttcaa
     4501 atgagggacc aggctttaca ttgataaatt caacatcttt ctctattgtt gcatgatcag
     4561 gtaggtttgt atatggggat ggagaaattt ttgatctgat cccgggtaca tcagtacatg
     4621 aggcatatct gacactggaa gagacttgtg cggacactgt tccagccctg gttgggattg
     4681 aatgtccaaa agatgaaaaa cttcctttgc ggaagatcta tgagaagcct aaaacaagat
     4741 gtttcactgt acttcctatg gaatataatc ttgtagttag gaggaaattt ctcaagtttg
     4801 tggtgtttat tatgaagaat cggcacagat tatcctgcca ggtgggcatc aatccatatg
     4861 gcatggaatg gagtcgcctg gcaatgagct tgcttgagaa aggaaacaac attttatgtt
     4921 gtgattacag ttcatttgat gggttgttga caaagcaagt tatgcatctc atgagtgaaa
     4981 tgatcaatga actgtgtggg ggatcttcgc gcctaaagca acagcgcacc aatttgttaa
     5041 tggcatgttg ttccaggtat gcattatgca agggagaagt gtggcgcgtt gaatgtggaa
     5101 ttccctctgg atttccattg actgtcattt gcaacagcat tttcaatgag ctattggtta
     5161 gatacagcta cattaagatt tgccaacaag cgcgtgtgcc agccaccata acatacggtt
     5221 ttagtacctt tgtaaagatg gtgacttatg gtgatgataa cttactgagc gttcagtctg
     5281 caatcactca cgtgtttgat ggaaccaagc tcaaggagtt tctgaaactc aatggcatca
     5341 ccatcactga cggcaaagac aaaacttccc ctgtgcttaa ttttcggaat ttggaagatt
     5401 gtgactttct caaaaggggc tttaagaaag aaagtgatgt agtgtgggtt ggaccagagg
     5461 aaaaggaatc gctgtgggca caactccatt atgtcacgac caacaatctt gaaaagcatg
     5521 aagcttattt ggtgaacgtt gtaaacgtga tcagggaatt gtacctacat gatccaagag
     5581 aggctgctga gttgcgtcgc agggcaatac aaaatgtaga cttcttgaag gaaaatccta
     5641 aagatttgcc cactatggct gctatcaagg atttctataa catgcagcga cagcaacaat
     5701 ttgtagactc caatgacaat ctggatagtc ttctgaatcc agattttttg tttgttgccc
     5761 cacatagaaa gatgcatgag gccgagatgg aattagtgcc aaagtggtat ctgagagatc
     5821 ttggcaaagc tccaataaat gttctaactg gagaagctga tcgaatttgt gttctggtta
     5881 atgcaagtat cccagaccat cttcttccag aaaaggtggt caatatatca tggccttatg
     5941 gacccggaag gggaggactg ccgactcatg gttgggcaca ggctaatctc tacaatccaa
     6001 atagtgctgt ggtgaagaaa ctgcgtacac ttgtgaatca aaatcctgat gatcgagtgg
     6061 atatatgttt taggcatgat gcagttccag ttgcgattgc aactatcatt ttcctggttc
     6121 atttaggaaa ggttaaaggg agaagcgcta atgaatattt gactaaaata attgatagtg
     6181 caaaatctct gaagttccta cctaaggagt gtgatatcat tttctgagtg tatttgagtt
     6241 tgtgtgtctc ttttcttact ctcagtctga ataactggca ttttcgccaa tttataaaat
     6301 tataatgtgt gattgttgtg tgtgatttct acagtacatg ttattacttt taaaaaaaaa
     6361 aaaaaaaaaa aaaaactagt ccgcaaaaat caccagtctc tctctacaaa tctatctctc
     6421 tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta gggttcttat
     6481 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
     6541 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacc tgcagaaagg
     6601 cgcgccaaag aattctttgg ctgaccatga ttacgaattc gtaatcatgg tcatagctgt
     6661 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     6721 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     6781 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     6841 cggggagagg cggtttgcgt attggctaga gcagcttgcc aatccagatc ccccgaatta
     6901 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     6961 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     7021 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     7081 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     7141 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     7201 acgatgacag agcgttgctg cctgtgatca ccgcggtttc aaaatcggct ccgtcgatac
     7261 tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt atttaaggtt
     7321 ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt cttggggtat
     7381 ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag aatatcaccg
     7441 gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg aatgtctcct
     7501 gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat gacggacagc
     7561 cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct atggctggaa
     7621 ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg gagcaatctg
     7681 ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga acaaagccct
     7741 gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat cgacatatcg
     7801 gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta cttactgaat
     7861 aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt taaagatccg
     7921 cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt cttttcccac
     7981 ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg ctttattgat
     8041 cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt ccggtcgatc
     8101 agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag
     8161 cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta gtacctagaa
     8221 tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     8281 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     8341 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     8401 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     8461 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     8521 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     8581 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     8641 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     8701 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     8761 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     8821 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     8881 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8941 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     9001 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     9061 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     9121 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     9181 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     9241 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     9301 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagggtgcct
     9361 tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt gtccgcgccc tggtagattg
     9421 cctggccgta ggccagccat ttttgagcgg ccagcggccg cgataggccg acgcgaagcg
     9481 gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt tttgcagctc ttcggctgtg
     9541 cgctggccag acagttatgc acaggccagg cgggttttaa gagttttaat aagttttaaa
     9601 gagttttagg cggaaaaatc gccttttttc tcttttatat cagtcactta catgtgtgac
     9661 cggttcccaa tgtacggctt tgggttccca atgtacgggt tccggttccc aatgtacggc
     9721 tttgggttcc caatgtacgt gctatccaca ggaaagagac cttttcgacc tttttcccct
     9781 gctagggcaa tttgccctag catctgctcc gtacattagg aaccggcgga tgcttcgccc
     9841 tcgatcaggt tgcggtagcg catgactagg atcgggccag cctgccccgc ctcctccttc
     9901 aaatcgtact ccggcaggtc atttgacccg atcagcttgc gcacggtgaa acagaacttc
     9961 ttgaactctc cggcgctgcc actgcgttcg tagatcgtct tgaacaacca tctggcttct
    10021 gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac ggccgatgcc gggatcgatc
    10081 aaaaagtaat cggggtgaac cgtcagcacg tccgggttct tgccttctgt gatctcgcgg
    10141 tacatccaat cagctagctc gatctcgatg tactccggcc gcccggtttc gctctttacg
    10201 atcttgtagc ggctaatcaa ggcttcaccc tcggataccg tcaccaggcg gccgttcttg
    10261 gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta accgaatgca ggtttctacc
    10321 aggtcgtctt tctgctttcc gccatcggct cgccggcaga acttgagtac gtccgcaacg
    10381 tgtggacgga acacgcggcc gggcttgtct cccttccctt cccggtatcg gttcatggat
    10441 tcggttagat gggaaaccgc catcagtacc aggtcgtaat cccacacact ggccatgccg
    10501 gccggccctg cggaaacctc tacgtgcccg tctggaagct cgtagcggat cacctcgcca
    10561 gctcgtcggt cacgcttcga cagacggaaa acggccacgt ccatgatgct gcgactatcg
    10621 cgggtgccca cgtcatagag catcggaacg aaaaaatctg gttgctcgtc gcccttgggc
    10681 ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc gggattcttt gcggattcga
    10741 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
    10801 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
    10861 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
    10921 cattgcaggg ccggcaggca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
    10981 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
    11041 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
    11101 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
    11161 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
    11221 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
    11281 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
    11341 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
    11401 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
    11461 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
    11521 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
    11581 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
    11641 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
    11701 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
    11761 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
    11821 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
    11881 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
    11941 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
    12001 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
    12061 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
    12121 gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc
    12181 gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg
    12241 cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg
    12301 gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg
    12361 ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct
    12421 ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg
    12481 agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg
    12541 gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc
    12601 atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg
    12661 ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg
    12721 tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc
    12781 tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc
    12841 gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag
    12901 cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa
    12961 gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg
    13021 gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact
    13081 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt actttgatcc
    13141 cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac
    13201 gcccttttaa atatccgatt attctaataa acgctctttt ctcttaggtt tacccgccaa
    13261 tatatcctgt caaacactga tagtttaaac tgaaggcggg aaacgacaat ctgatccaag
    13321 ctcaagctgc tctagcattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
    13381 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
    13441 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
    13501 gttaggccta aacctgcagg tcaacatggt ggagcacgac acacttgtct actccaaaaa
    13561 tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac aaagggtaat
    13621 atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg tgaagatagt
    13681 ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ccatcgttga
    13741 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
    13801 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata acatggtgga
    13861 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
    13921 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
    13981 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
    14041 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
    14101 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
    14161 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
    14221 gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggaccg gtt
//
