LOCUS       sequence-363110-        9923 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  U6-crKlf4-CAG-hyperdCas12a-miniVPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9923)
  AUTHORS   Guo LY, Bian J, Davis AE, Liu P, Kempton HR, Zhang X, Chemparathy A,
            Gu B, Lin X, Rane DA, Xu X, Jamiolkowski RM, Hu Y, Wang S, Qi LS
  TITLE     Multiplexed genome regulation in vivo with hyper-efficient Cas12a.
  JOURNAL   Nat Cell Biol. 2022 Apr;24(4):590-600. doi: 
            10.1038/s41556-022-00870-7. Epub 2022 Apr 12.
  PUBMED    35414015
REFERENCE   2  (bases 1 to 9923)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-022-00870-7"; journalName: "Nat Cell Biol"; date: 
            "2022-04"; volume: "24"; issue: "4"; pages: "590-600"
COMMENT     Sequence Label: pSLQ10714
FEATURES             Location/Qualifiers
     source          1..9923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..304
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     56..76
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     227..246
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        393..772
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1047..2063
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1987..2009
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2071..2090
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2160..5843
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFRNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIRVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGRGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKRIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5850..5876
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5856..5882
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             5895..5921
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5931..5957
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             5994..6143
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6150..6170
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6303..6659
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     primer_bind     complement(6971..6990)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7036..7091
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7090..7109)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7452..7468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7452..7468)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7465..7487)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7476..7492
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7500..7506,7507..7524,7525..7530))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7545..7566
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7625..7820
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7671..7806
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7826..7960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7863..7882)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7917..7936
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8028..8045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8199..8787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8279..8298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8958..9749,9750..9818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9581..9600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9819..9923)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagc aattggaggg
       61 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
      121 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
      181 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      241 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      301 caccgaattt ctactaagtg tagatatagc aggcgcggaa ccccttcttt tttttaagct
      361 tgggccgctc gagggatcca ttaggcggcc gcgacattga ttattgacta gttattaata
      421 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      481 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
      541 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      601 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      661 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      721 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtcgaggt
      781 gagccccacg ttctgcttca ctctccccat ctcccccccc tccccacccc caattttgta
      841 tttatttatt ttttaattat tttgtgcagc gatgggggcg gggggggggg cgcgcgccag
      901 gcggggcggg gsggggsgrg gggsggggsg gggsgrggsg grgrggkgsg gsggcrgcca
      961 atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct
     1021 ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc
     1081 gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg
     1141 tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc
     1201 ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg
     1261 gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct
     1321 ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca
     1381 gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg
     1441 ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt
     1501 cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac ggcccggctt
     1561 cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc ggggggkggc
     1621 ggcaggkggg ggtgccgggc ggggcggggc cgccycgggc cggggrgggc tcgggggrgg
     1681 ggcgcggcgg cccccggagc gccggcggct gtcgaggcgc ggcgagccgc agccattgcc
     1741 ttttatggta atcgtgcgag agggcgcagg gacttccttt gtcccaaatc tgtgcggagc
     1801 cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cggggcgaag cggtgcggcg
     1861 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     1921 tccctctcca gcctcggggc tgtccgcggg gggacggctg ccttcggggg ggacggggca
     1981 gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct aaccatgttc
     2041 atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg ctgtctcatc
     2101 attttggcaa agaattctgc agtcgacggt accgcgggcc cgggatccac cgggccacca
     2161 tgagcaagct ggagaagttt acaaactgct actccctgtc taagaccctg aggttcaagg
     2221 ccatccctgt gggcaagacc caggagaaca tcgacaataa gcggctgctg gtggaggacg
     2281 agaagagagc cgaggattat aagggcgtga agaagctgct ggatcgctac tatctgtctt
     2341 ttatcaacga cgtgctgcac agcatcaagc tgaagaatct gaacaattac atcagcctgt
     2401 tccggaagaa aaccagaacc gagaaggaga ataaggagct ggagaacctg gagatcaatc
     2461 tgcggaagga gatcgccaag gccttcaagg gcaacgaggg ctacaagtcc ctgtttaaga
     2521 aggatatcat cgagacaatc ctgccagagt tcctggacga taaggacgag atcgccctgg
     2581 tgaacagctt caatggcttt accacagcct tcaccggctt ctttcgtaac agagagaata
     2641 tgttttccga ggaggccaag agcacatcca tcgccttcag gtgtatcaac gagaatctga
     2701 cccgctacat ctctaatatg gacatcttcg agaaggtgga cgccatcttt gataagcacg
     2761 aggtgcagga gatcaaggag aagatcctga acagcgacta tgatgtggag gatttctttg
     2821 agggcgagtt ctttaacttt gtgctgacac aggagggcat ccgcgtgtat aacgccatca
     2881 tcggcggctt cgtgaccgag agcggcgaga agatcaaggg cctgaacgag tacatcaacc
     2941 tgtataatca gaaaaccaag cagaagctgc ctaagtttaa gccactgtat aagcaggtgc
     3001 tgagcgatcg ggagtctctg agcttctacg gcaggggcta tacatccgat gaggaggtgc
     3061 tggaggtgtt tagaaacacc ctgaacaaga acagcgagat cttcagctcc atcaagaagc
     3121 tggagaagct gttcaagaat tttgacgagt actctagcgc cggcatcttt gtgaagaacg
     3181 gccccgccat cagcacaatc tccaagcgta tcttcggcga gtggaacgtg atccgggaca
     3241 agtggaatgc cgagtatgac gatatccacc tgaagaagaa ggccgtggtg accgagaagt
     3301 acgaggacga tcggagaaag tccttcaaga agatcggctc cttttctctg gagcagctgc
     3361 aggagtacgc cgacgccgat ctgtctgtgg tggagaagct gaaggagatc atcatccaga
     3421 aggtggatga gatctacaag gtgtatggat cctctgagaa gctgttcgac gccgattttg
     3481 tgctggagaa gagcctgaag aagaacgacg ccgtggtggc catcatgaag gacctgctgg
     3541 attctgtgaa gagcttcgag aattacatca aggccttctt tggcgagggc aaggagacaa
     3601 acagggacga gtccttctat ggcgattttg tgctggccta cgacatcctg ctgaaggtgg
     3661 accacatcta cgatgccatc cgcaattatg tgacccagaa gccctactct aaggataagt
     3721 tcaagctgta ttttcagaac cctcagttca tgggcggctg ggacaaggat aaggagacag
     3781 actatcgggc caccatcctg agatacggct ccaagtacta tctggccatc atggataaga
     3841 agtacgccaa gtgcctgcag aagatcgaca aggacgatgt gaacggcaat tacgagaaga
     3901 tcaactataa gctgctgccc ggccctaata agatgctgcc aaaggtgttc ttttctaaga
     3961 agtggatggc ctactataac cccagcgagg acatccagaa gatctacaag aatggcacat
     4021 tcaagaaggg cgatatgttt aacctgaatg actgtcacaa gctgatcgac ttctttaagg
     4081 atagcatctc ccggtatcca aagtggtcca atgcctacga tttcaacttt tctgagacag
     4141 agaagtataa ggacatcgcc ggcttttaca gagaggtgga ggagcagggc tataaggtga
     4201 gcttcgagtc tgccagcaag aaggaggtgg ataagctggt ggaggagggc aagctgtata
     4261 tgttccagat ctataacaag gacttttccg ataagtctca cggcacaccc aatctgcaca
     4321 ccatgtactt caagctgctg tttgacgaga acaatcacgg acagatcagg ctgagcggag
     4381 gagcagagct gttcatgagg cgcgcctccc tgaagaagga ggagctggtg gtgcacccag
     4441 ccaactcccc tatcgccaac aagaatccag ataatcccaa gaaaaccaca accctgtcct
     4501 acgacgtgta taaggataag aggttttctg aggaccagta cgagctgcac atcccaatcg
     4561 ccatcaataa gtgccccaag aacatcttca agatcaatac agaggtgcgc gtgctgctga
     4621 agcacgacga taacccctat gtgatcggca tcgccagggg cgagcgcaat ctgctgtata
     4681 tcgtggtggt ggacggcaag ggcaacatcg tggagcagta ttccctgaac gagatcatca
     4741 acaacttcaa cggcatcagg atcaagacag attaccactc tctgctggac aagaaggaga
     4801 aggagaggtt cgaggcccgc cagaactgga cctccatcga gaatatcaag gagctgaagg
     4861 ccggctatat ctctcaggtg gtgcacaaga tctgcgagct ggtggagaag tacgatgccg
     4921 tgatcgccct ggaggacctg aactctggct ttaagaatag ccgcgtgaag gtggagaagc
     4981 aggtgtatca gaagttcgag aagatgctga tcgataagct gaactacatg gtggacaaga
     5041 agtctaatcc ttgtgcaaca ggcggcgccc tgaagggcta tcagatcacc aataagttcg
     5101 agagctttaa gtccatgtct acccagaacg gcttcatctt ttacatccct gcctggctga
     5161 catccaagat cgatccatct accggctttg tgaacctgct gaaaaccaag tataccagca
     5221 tcgccgattc caagaagttc atcagctcct ttgacaggat catgtacgtg cccgaggagg
     5281 atctgttcga gtttgccctg gactataaga acttctctcg cacagacgcc gattacatca
     5341 agaagtggaa gctgtactcc tacggcaacc ggatcagaat cttccggaat cctaagaaga
     5401 acaacgtgtt cgactgggag gaggtgtgcc tgaccagcgc ctataaggag ctgttcaaca
     5461 agtacggcat caattatcag cagggcgata tcagagccct gctgtgcgag cagtccgaca
     5521 aggccttcta ctctagcttt atggccctga tgagcctgat gctgcagatg cggaacagca
     5581 tcacaggccg caccgacgtg gattttctga tcagccctgt gaagaactcc gacggcatct
     5641 tctacgatag ccggaactat gaggcccagg agaatgccat cctgccaaag aacgccgacg
     5701 ccaatggcgc ctataacatc gccagaaagg tgctgtgggc catcggccag ttcaagaagg
     5761 ccgaggacga gaagctggat aaggtgaaga tcgccatctc taacaaggag tggctggagt
     5821 acgcccagac cagcgtgaag cacggatcct atccctatga cgtgcccgat tatgccagcc
     5881 tgggcagcgg ctcccctgct gctaaacgtg ttaagcttga tgggggtagc ccggcagcca
     5941 agagagtcaa actcgacgac ggcattggta gtgggagcaa cggcagcagc ctcgatgctt
     6001 tagacgattt tgacttagat atgcttggtt cagacgcgtt agacgacttc gacctagaca
     6061 tgttaggctc agatgcattg gacgacttcg atttagatat gttgggctcc gatgccctag
     6121 atgactttga tctagatatg ctaggtagtc ccaaaaagaa gaggaaagtg ggatccgtcc
     6181 ttccgcaagc gcctgcccca gcgcctgcac cggcaatggt gtccgccctc gcacaggccc
     6241 ctgcgcccgt ccccgtgctc gcgcctggac cgccccaggc ggtcgctcca ccggctccga
     6301 agccgacgca ggccggagag ggaacactct ccgaagcact tcttcaactc cagtttgatg
     6361 acgaggatct tggagcactc cttggaaact cgacagaccc tgcggtgttt accgacctcg
     6421 cgtcagtaga taactccgaa tttcagcagc ttttgaacca gggtatcccg gtcgcgccac
     6481 atacaacgga gcccatgttg atggaatacc ccgaagcaat cacgagactt gtgacgggag
     6541 cgcagcggcc tcccgatccc gcacccgcac ctttgggggc acctggcctc cctaacggac
     6601 ttttgagcgg cgacgaggat ttctcctcca tcgccgatat ggatttctca gccttgctgt
     6661 caggctctgg cagcggcagc gacctttccc atccgccccc aaggggccat ctggatgagc
     6721 tgacaaccac acttgagtcc atgaccgagg atctgaacct ggactcaccc ctgaccccgg
     6781 aattgaacga gattctggat accttcctga acgacgagtg cctcttgcat gccatgcata
     6841 tcagcacagg actgtccatc ttcgacacat ctctgtttta gtgtacaggc cgcactcctc
     6901 aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat
     6961 accactgaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     7021 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     7081 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     7141 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     7201 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     7261 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     7321 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     7381 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
     7441 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     7501 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     7561 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     7621 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     7681 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     7741 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     7801 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     7861 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7921 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     7981 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     8041 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     8101 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     8161 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     8221 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     8281 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     8341 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     8401 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     8461 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     8521 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     8581 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     8641 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     8701 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     8761 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     8821 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     8881 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     8941 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     9001 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     9061 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     9121 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     9181 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     9241 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     9301 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     9361 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     9421 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     9481 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     9541 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     9601 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     9661 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     9721 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     9781 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     9841 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     9901 aataaacaaa taggggttcc gcg
//
