LOCUS       sequence-363113-       11095 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of a golgi-residential PEN2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11095)
  AUTHORS   Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu 
            Y, Long S, Feng JW, Li C, Zhang C, Xie C, Wu Y, Xu Z, Chen J, Yu Y, 
            Huang X, He Y, Yao L, Zhang L, Zhu M, Wang W, Wang ZC, Zhang M, Bao 
            Y, Jia W, Lin SY, Ye Z, Piao HL, Deng X, Zhang CS, Lin SC
  TITLE     Low-dose metformin targets the lysosomal AMPK pathway through PEN2.
  JOURNAL   Nature. 2022 Mar;603(7899):159-165. doi: 10.1038/s41586-022-04431-8.
            Epub 2022 Feb 23.
  PUBMED    35197629
REFERENCE   2  (bases 1 to 11095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04431-8"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7899"; pages: "159-165"
COMMENT     Sequence Label: pBoBi-hGOLGA2-C-PEN2
FEATURES             Location/Qualifiers
     source          1..11095
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(88..108)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        93..422
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      273..408
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     335..354
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        470..517
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             536..910
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1077..1096)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1131..1150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1210..1226)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1210..1226)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1223..1245)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1234..1250
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1258..1264,1265..1282,1283..1288))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1303..1324
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1441..1458)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1612..2200)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1692..1711)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2371..3162,3163..3231))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2994..3013
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3232..3336)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3411..3430)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3602..3981
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3983..4181
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4199..4379
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4426..4551
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5044..5277
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5462..5506
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5758..5874
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        5897..6200
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6201..6404
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6401..6425
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6503..6529
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6536..6562
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6566..6592
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      7671..7680
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          8863..9092
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     9075..9094
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    9269..9857
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9322..9342)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9740..9751)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10264..10444
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10470..10487)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10476..10700
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10746..11095,1..79)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10833..10852)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11043..11064
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
       61 caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc
      121 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
      181 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      241 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
      301 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
      361 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
      421 aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta
      481 atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc
      541 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt
      601 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt
      661 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac
      721 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt
      781 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg
      841 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga
      901 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg
      961 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     1021 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     1081 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     1141 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     1201 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1261 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1321 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1381 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     1441 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     1501 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     1561 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     1621 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     1681 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     1741 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     1801 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     1861 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     1921 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     1981 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2041 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2101 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2161 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2221 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2281 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2341 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     2401 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     2461 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     2521 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     2581 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     2641 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     2701 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     2761 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     2821 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     2881 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     2941 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3001 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3061 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3121 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3181 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3241 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3301 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3361 tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc
     3421 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
     3481 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
     3541 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
     3601 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
     3661 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     3721 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     3781 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     3841 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     3901 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     3961 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     4021 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     4081 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     4141 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg cctgtactgg
     4201 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4261 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4321 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4381 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     4441 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     4501 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     4561 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     4621 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     4681 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     4741 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     4801 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     4861 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     4921 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     4981 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     5041 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     5101 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     5161 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     5221 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     5281 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     5341 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     5401 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     5461 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     5521 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     5581 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     5641 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     5701 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcaacttt
     5761 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     5821 aacagacata caaactaaag aattacaaaa acaaattaca aaattcaaaa ttttatcgat
     5881 aagcttggga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     5941 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     6001 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     6061 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     6121 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     6181 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     6241 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     6301 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     6361 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     6421 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgactgagct
     6481 ccttaaggtt aacgccacca tgtaccctta tgacgtacct gactatgctg gcgcctaccc
     6541 ttatgacgta cctgactatg ctggataccc ttatgacgta cctgactatg ctggatctag
     6601 agaattcgga tccatggagt cggttagaca actacaaatg gagagagata aatatgcgga
     6661 gaatctcaaa ggagagagcg ccatgtggcg gcagaggatg cagcagatgt cagagcaggt
     6721 gcacacattg agagaggaga aggaatgtag catgagtcgg gtacaggagc tggagacgag
     6781 cttggctgaa ctgaggaacc agatggctga acccccgccc ccagagcccc cagcagggcc
     6841 ctccgaggtg gagcagcagc tacaagcgga ggctgagcac ctgcggaagg agctggaggg
     6901 tctggcagga cagcttcaag cccaggtgca agacaatgag ggcttgagtc gcctgaaccg
     6961 ggagcaggag gagaggctgc tggagctgga gcgggcggcc gagctctggg gggagcaggc
     7021 ggaggcgcgc aggcaaatcc tggagaccat gcagaacgac cgcactacca tcagccgcgc
     7081 actctcccag aaccgggagc tcaaggagca gctggctgag ctgcagagcg gatttgtaaa
     7141 gctgactaat gagaacatgg agatcaccag cgcactgcag tcggagcagc acgtcaagag
     7201 ggagctggga aagaagctgg gcgagctgca ggagaagctg agcgagctga aggaaacggt
     7261 ggagctgaag agccaagagg ctcaaagtct gcagcagcag cgagaccagt acctgggaca
     7321 cctgcagcag tatgtggccg cctatcagca gctgacctct gagaaggagg tgctgcataa
     7381 tcagctactg ctgcagaccc agctcgtgga ccagctgcag cagcaggaag ctcagggcaa
     7441 agcggtggcc gagatggccc gccaagagtt gcaggaaacc caggagcgcc tggaagctgc
     7501 cacccagcag aatcagcagc tacgggccca gttgagcctc atggctcacc ctggggaagg
     7561 agatggactg gaccgggagg aggaggagga tgaggaggag gaggaggagg aggcggtggc
     7621 agtacctcag cccatgccaa gcatcccgga ggacctggag agccgggaag ccatggtggc
     7681 atttttcaac tcagctgtag ccagtgccga ggaggagcag gcaaggctac gtgggcagct
     7741 gaaggagcaa agggtgcgct gccggcgcct ggctcacctg ctggcctcgg cccagaagga
     7801 gcctgaggca gcagccccag ccccagggac cgggggtgat tctgtgtgtg gggagaccca
     7861 ccgggccctg cagggggcca tggagaagct gcagagccgc tttatggagc tcatgcagga
     7921 gaaggcagac ctgaaggaga gggtagagga actggaacat cgctgcatcc agctttctgg
     7981 agagacagac accattggag agtacattgc actgtaccag agccagaggg cagtgctgaa
     8041 ggagcggcac cgggagaagg aggagtacat cagcaggctg gcccaagaca aggaggagat
     8101 gaaggtgaag ctgctggagc tgcaggagct ggtcttacgg cttgtgggcg accgcaacga
     8161 gtggcatggc agattcctgg cagctgccca gaaccctgct gatgagccca cttcaggggc
     8221 cccagccccc caggaacttg gggctgccaa ccagcagggt gatctttgcg aggtgagcct
     8281 cgccggcagt gtggagcctg cccaaggaga ggccagggag ggttctcccc gtgacaaccc
     8341 cactgcacag cagatcatgc agctgcttcg tgagatgcag aacccccggg agcgcccagg
     8401 cttgggcagc aacccctgca ttcctttttt ttaccgggct gacgagaatg atgaggtgaa
     8461 gatcactgtc atcatgaacc tggagcgagt gtccaatgag gagaaattga acctgtgccg
     8521 gaagtactac ctgggggggt ttgctttcct gccttttctc tggttggtca acatcttctg
     8581 gttcttccga gaggccttcc ttgtcccagc ctacacagaa cagagccaaa tcaaaggcta
     8641 tgtctggcgc tcagctgtgg gcttcctctt ctgggtgata gtgctcacct cctggatcac
     8701 catcttccag atctaccggc cccgctgggg tgcccttggg gactacctct ccttcaccat
     8761 acccctgggc acccccctcg agccatggaa gcttgatatc taactgactg aaccggtggt
     8821 accgaggaat taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa
     8881 aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat
     8941 tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa
     9001 tctttttgtt gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga
     9061 caatgacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt
     9121 cgagcatgca tctagggcgg ccaattccgc cgatcctgga aaaacatgga gcaatcacaa
     9181 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     9241 aggtgggttt tccagtcaca cctcagacaa tcaacctctg gattacaaaa tttgtgaaag
     9301 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     9361 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     9421 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     9481 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     9541 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     9601 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     9661 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     9721 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     9781 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     9841 ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag
     9901 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     9961 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    10021 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
    10081 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
    10141 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
    10201 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
    10261 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    10321 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    10381 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    10441 agcagggccg ctttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
    10501 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
    10561 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
    10621 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
    10681 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
    10741 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    10801 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    10861 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    10921 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    10981 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    11041 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attct
//
