LOCUS       sequence-363115-       10270 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To (stably) express a mutant of ATP6AP1 lacking the interacting 
            domain with PEN2; lentiviral expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Ma T, Tian X, Zhang B, Li M, Wang Y, Yang C, Wu J, Wei X, Qu Q, Yu 
            Y, Long S, Feng JW, Li C, Zhang C, Xie C, Wu Y, Xu Z, Chen J, Yu Y, 
            Huang X, He Y, Yao L, Zhang L, Zhu M, Wang W, Wang ZC, Zhang M, Bao 
            Y, Jia W, Lin SY, Ye Z, Piao HL, Deng X, Zhang CS, Lin SC
  TITLE     Low-dose metformin targets the lysosomal AMPK pathway through PEN2.
  JOURNAL   Nature. 2022 Mar;603(7899):159-165. doi: 10.1038/s41586-022-04431-8.
            Epub 2022 Feb 23.
  PUBMED    35197629
REFERENCE   2  (bases 1 to 10270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04431-8"; journalName: "Nature"; date: 
            "2022-03"; volume: "603"; issue: "7899"; pages: "159-165"
COMMENT     Sequence Label: pBoBi-hAP1 Delta-420-440
FEATURES             Location/Qualifiers
     source          1..10270
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      180..315
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     242..261
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        377..424
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             443..817
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    947..1068
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(984..1003)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1038..1057
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1117..1133)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1117..1133)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1130..1152)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1141..1157
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1165..1171,1172..1189,1190..1195))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1210..1231
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1348..1365)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1519..2107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1599..1618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2278..3069,3070..3138))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2901..2920
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3139..3243)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3318..3337)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3509..3888
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3890..4088
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4106..4286
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4333..4458
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4951..5184
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5369..5413
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5665..5781
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        5804..6107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6108..6311
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6308..6332
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             7784..7810
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7817..7843
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7847..7873
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          7945..8174
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     8157..8176
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    8351..8939
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8404..8424)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8822..8833)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9346..9526
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9552..9569)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9558..9782
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9828..10256
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9915..9934)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10125..10146
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(10265..10270,1..15))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(10270,1..329)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
       61 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
      121 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
      181 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
      241 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
      301 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
      361 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
      421 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
      481 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
      541 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
      601 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
      661 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
      721 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
      781 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
      841 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
      901 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
      961 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1021 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     1081 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1141 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1201 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1261 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1321 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1381 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1441 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1501 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1561 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1621 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1681 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1741 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1801 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1861 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1921 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1981 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2041 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2101 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2161 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2221 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2281 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2341 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2401 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2461 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2521 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2581 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2641 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2701 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2761 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2821 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     2881 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     2941 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3001 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3061 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3121 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3181 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3241 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     3301 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     3361 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     3421 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     3481 cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa
     3541 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     3601 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     3661 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     3721 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     3781 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     3841 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     3901 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     3961 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     4021 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     4081 ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
     4141 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4201 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4261 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     4321 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     4381 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     4441 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     4501 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     4561 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     4621 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     4681 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     4741 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     4801 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     4861 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     4921 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     4981 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     5041 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     5101 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     5161 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     5221 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     5281 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     5341 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     5401 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     5461 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     5521 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     5581 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     5641 cattcgatta gtgaacggat caacttttaa aagaaaaggg gggattgggg ggtacagtgc
     5701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     5761 aattacaaaa ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg
     5821 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     5881 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     5941 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     6001 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     6061 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     6121 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     6181 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     6241 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     6301 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     6361 acctccatag aagacaccga ctgagctcct taaggttaac tctagagaat tcggatccat
     6421 gatggcggcc atggcgacgg ctcgagtgcg gatggggccg cggtgcgccc aggcgctctg
     6481 gcgcatgccg tggctgccgg tgtttttgtc gttggcggcg gcggcggcgg cggcagcggc
     6541 ggagcagcag gtcccgctgg tgctgtggtc gagtgaccgg gacttgtggg ctcctgcggc
     6601 cgacactcat gaaggccaca tcaccagcga cttgcagctc tctacctact tagatcccgc
     6661 cctggagctg ggtcccagga atgtgctgct gttcctgcag gacaagctga gcattgagga
     6721 tttcacagca tatggcggtg tgtttggaaa caagcaggac agcgcctttt ctaacctaga
     6781 gaatgccctg gacctggccc cctcctcact ggtgcttcct gccgtcgact ggtatgcagt
     6841 cagcactctg accacttacc tgcaggagaa gctcggggcc agccccttgc atgtggacct
     6901 ggccaccctg cgggagctga agctcaatgc cagcctccct gctctgctgc tcattcgcct
     6961 gccctacaca gccagctctg gtctgatggc acccagggaa gtcctcacag gcaacgatga
     7021 ggtcatcggg caggtcctga gcacactcaa gtccgaagat gtcccataca cagcggccct
     7081 cacagcggtc cgcccttcca gggtggcccg tgatgtagcc gtggtggccg gagggctagg
     7141 tcgccagctg ctacaaaaac agccagtatc acctgtgatc catcctcctg tgagttacaa
     7201 tgacaccgct ccccggatcc tgttctgggc ccaaaacttc tctgtggcgt acaaggacca
     7261 gtgggaggac ctgactcccc tcacctttgg ggtgcaggaa ctcaacctga ctggctcctt
     7321 ctggaatgac tcctttgcca ggctctcact gacctatgaa cgactctttg gtaccacagt
     7381 gacattcaag ttcattctgg ccaaccgcct ctacccagtg tctgcccggc actggtttac
     7441 catggagcgc ctcgaagtcc acagcaatgg ctccgtcgcc tacttcaatg cttcccaggt
     7501 cacagggccc agcatctact ccttccactg cgagtatgtc agcagcctga gcaagaaggg
     7561 tagtctcctc gtggcccgca cgcagccctc tccctggcag atgatgcttc aggacttcca
     7621 gatccaggct ttcaacgtaa tgggggagca gttctcctac gccagcgact gtgccaccta
     7681 tggcctgcac atgatcctca gcctcaagac catggatcgc tttgatgacc acaagggccc
     7741 cactatttct ttgacccaga ttgtgctcga gccatggaag ctttaccctt atgacgtacc
     7801 tgactatgct ggcgcctacc cttatgacgt acctgactat gctggatacc cttatgacgt
     7861 acctgactat gctggagata tctaactgac tgaaccggtg gtaccgagga attaattcgc
     7921 tgtctgcgag ggccagctgt tggggtgagt actccctctc aaaagcgggc atgacttctg
     7981 cgctaagatt gtcagtttcc aaaaacgagg aggatttgat attcacctgg cccgcggtga
     8041 tgcctttgag ggtggccgcg tccatctggt cagaaaagac aatctttttg ttgtcaagct
     8101 tgaggtgtgg caggcttgag atctggccat acacttgagt gacaatgaca tccactttgc
     8161 ctttctctcc acaggtgtcc actcccaggt ccaactgcag gtcgagcatg catctagggc
     8221 ggccaattcc gccgatcctg gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     8281 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     8341 cacctcagac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     8401 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     8461 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     8521 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     8581 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     8641 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     8701 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc
     8761 atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     8821 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     8881 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     8941 tggaattcga gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     9001 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     9061 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     9121 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     9181 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     9241 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     9301 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     9361 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9421 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9481 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc cgctttaaac
     9541 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     9601 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     9661 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     9721 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     9781 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     9841 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     9901 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     9961 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    10021 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    10081 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    10141 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    10201 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
    10261 ctgtggaatg
//
