LOCUS       sequence-363117-       10144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  U6-SKSKSO(crRNA array)-CAG-dCas12a(WT)-miniVPR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10144)
  AUTHORS   Guo LY, Bian J, Davis AE, Liu P, Kempton HR, Zhang X, Chemparathy A,
            Gu B, Lin X, Rane DA, Xu X, Jamiolkowski RM, Hu Y, Wang S, Qi LS
  TITLE     Multiplexed genome regulation in vivo with hyper-efficient Cas12a.
  JOURNAL   Nat Cell Biol. 2022 Apr;24(4):590-600. doi: 
            10.1038/s41556-022-00870-7. Epub 2022 Apr 12.
  PUBMED    35414015
REFERENCE   2  (bases 1 to 10144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41556-022-00870-7"; journalName: "Nat Cell Biol"; date: 
            "2022-04"; volume: "24"; issue: "4"; pages: "590-600"
COMMENT     Sequence Label: pSLQ10871
FEATURES             Location/Qualifiers
     source          1..10144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..304
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     56..76
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     227..246
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        614..993
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          1268..2284
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     2208..2230
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     2292..2311
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             2381..6064
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIARGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             6071..6097
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6077..6103
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             6116..6142
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             6152..6178
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             6215..6364
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             6371..6391
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6524..6880
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     primer_bind     complement(7192..7211)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7257..7312
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7311..7330)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7673..7689)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7673..7689)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7686..7708)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7697..7713
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7721..7727,7728..7745,7746..7751))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7766..7787
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7846..8041
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7892..8027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8047..8181
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8084..8103)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8138..8157
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8249..8266)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8420..9008)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8500..8519)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9179..9970,9971..10039))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9802..9821
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10040..10144)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgggtc gacattgatt attgactagc aattggaggg
       61 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
      121 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
      181 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      241 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      301 caccgaattt ctactaagtg tagatttccc tgacagcccc catcacataa tttctactaa
      361 gtgtagatat agcaggcgcg gaaccccttc taatttctac taagtgtaga tagcaacagg
      421 tcacggcgca cgaatttcta ctaagtgtag atgctaccat ggcaacgcgc agtggaattt
      481 ctactaagtg tagataacaa gttaatagac aaccatccaa tttctactaa gtgtagattc
      541 ccacccccac agctctgctc caatttctac taagtgtaga tttttttact cgagggatcc
      601 attaggcggc cgcgacattg attattgact agttattaat agtaatcaat tacggggtca
      661 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      721 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      781 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      841 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      901 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      961 tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc
     1021 actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta
     1081 ttttgtgcag cgatgggggc gggggggggg gcgcgcgcca ggcggggcgg ggsggggsgr
     1141 ggggsggggs ggggsgrggs ggrgrggkgs ggsggcrgcc aatcagagcg gcgcgctccg
     1201 aaagtttcct tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg
     1261 gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg
     1321 ccgcccgccc cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc
     1381 ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct
     1441 gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg
     1501 tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg
     1561 tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc
     1621 ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg
     1681 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc
     1741 cctgcacccc cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac
     1801 ggggcgtggc gcggggctcg ccgtgccggg cggggggkgg cggcaggkgg gggtgccggg
     1861 cggggcgggg ccgccycggg ccggggrggg ctcgggggrg gggcgcggcg gcccccggag
     1921 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1981 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg
     2041 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg
     2101 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg
     2161 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg
     2221 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct
     2281 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattctg
     2341 cagtcgacgg taccgcgggc ccgggatcca ccgggccacc atgagcaagc tggagaagtt
     2401 tacaaactgc tactccctgt ctaagaccct gaggttcaag gccatccctg tgggcaagac
     2461 ccaggagaac atcgacaata agcggctgct ggtggaggac gagaagagag ccgaggatta
     2521 taagggcgtg aagaagctgc tggatcgcta ctatctgtct tttatcaacg acgtgctgca
     2581 cagcatcaag ctgaagaatc tgaacaatta catcagcctg ttccggaaga aaaccagaac
     2641 cgagaaggag aataaggagc tggagaacct ggagatcaat ctgcggaagg agatcgccaa
     2701 ggccttcaag ggcaacgagg gctacaagtc cctgtttaag aaggatatca tcgagacaat
     2761 cctgccagag ttcctggacg ataaggacga gatcgccctg gtgaacagct tcaatggctt
     2821 taccacagcc ttcaccggct tctttgataa cagagagaat atgttttccg aggaggccaa
     2881 gagcacatcc atcgccttca ggtgtatcaa cgagaatctg acccgctaca tctctaatat
     2941 ggacatcttc gagaaggtgg acgccatctt tgataagcac gaggtgcagg agatcaagga
     3001 gaagatcctg aacagcgact atgatgtgga ggatttcttt gagggcgagt tctttaactt
     3061 tgtgctgaca caggagggca tcgacgtgta taacgccatc atcggcggct tcgtgaccga
     3121 gagcggcgag aagatcaagg gcctgaacga gtacatcaac ctgtataatc agaaaaccaa
     3181 gcagaagctg cctaagttta agccactgta taagcaggtg ctgagcgatc gggagtctct
     3241 gagcttctac ggcgagggct atacatccga tgaggaggtg ctggaggtgt ttagaaacac
     3301 cctgaacaag aacagcgaga tcttcagctc catcaagaag ctggagaagc tgttcaagaa
     3361 ttttgacgag tactctagcg ccggcatctt tgtgaagaac ggccccgcca tcagcacaat
     3421 ctccaaggat atcttcggcg agtggaacgt gatccgggac aagtggaatg ccgagtatga
     3481 cgatatccac ctgaagaaga aggccgtggt gaccgagaag tacgaggacg atcggagaaa
     3541 gtccttcaag aagatcggct ccttttctct ggagcagctg caggagtacg ccgacgccga
     3601 tctgtctgtg gtggagaagc tgaaggagat catcatccag aaggtggatg agatctacaa
     3661 ggtgtatggc tcctctgaga agctgttcga cgccgatttt gtgctggaga agagcctgaa
     3721 gaagaacgac gccgtggtgg ccatcatgaa ggacctgctg gattctgtga agagcttcga
     3781 gaattacatc aaggccttct ttggcgaggg caaggagaca aacagggacg agtccttcta
     3841 tggcgatttt gtgctggcct acgacatcct gctgaaggtg gaccacatct acgatgccat
     3901 ccgcaattat gtgacccaga agccctactc taaggataag ttcaagctgt attttcagaa
     3961 ccctcagttc atgggcggct gggacaagga taaggagaca gactatcggg ccaccatcct
     4021 gagatacggc tccaagtact atctggccat catggataag aagtacgcca agtgcctgca
     4081 gaagatcgac aaggacgatg tgaacggcaa ttacgagaag atcaactata agctgctgcc
     4141 cggccctaat aagatgctgc caaaggtgtt cttttctaag aagtggatgg cctactataa
     4201 ccccagcgag gacatccaga agatctacaa gaatggcaca ttcaagaagg gcgatatgtt
     4261 taacctgaat gactgtcaca agctgatcga cttctttaag gatagcatct cccggtatcc
     4321 aaagtggtcc aatgcctacg atttcaactt ttctgagaca gagaagtata aggacatcgc
     4381 cggcttttac agagaggtgg aggagcaggg ctataaggtg agcttcgagt ctgccagcaa
     4441 gaaggaggtg gataagctgg tggaggaggg caagctgtat atgttccaga tctataacaa
     4501 ggacttttcc gataagtctc acggcacacc caatctgcac accatgtact tcaagctgct
     4561 gtttgacgag aacaatcacg gacagatcag gctgagcgga ggagcagagc tgttcatgag
     4621 gcgcgcctcc ctgaagaagg aggagctggt ggtgcaccca gccaactccc ctatcgccaa
     4681 caagaatcca gataatccca agaaaaccac aaccctgtcc tacgacgtgt ataaggataa
     4741 gaggttttct gaggaccagt acgagctgca catcccaatc gccatcaata agtgccccaa
     4801 gaacatcttc aagatcaata cagaggtgcg cgtgctgctg aagcacgacg ataaccccta
     4861 tgtgatcggc atcgccaggg gcgagcgcaa tctgctgtat atcgtggtgg tggacggcaa
     4921 gggcaacatc gtggagcagt attccctgaa cgagatcatc aacaacttca acggcatcag
     4981 gatcaagaca gattaccact ctctgctgga caagaaggag aaggagaggt tcgaggcccg
     5041 ccagaactgg acctccatcg agaatatcaa ggagctgaag gccggctata tctctcaggt
     5101 ggtgcacaag atctgcgagc tggtggagaa gtacgatgcc gtgatcgccc tggaggacct
     5161 gaactctggc tttaagaata gccgcgtgaa ggtggagaag caggtgtatc agaagttcga
     5221 gaagatgctg atcgataagc tgaactacat ggtggacaag aagtctaatc cttgtgcaac
     5281 aggcggcgcc ctgaagggct atcagatcac caataagttc gagagcttta agtccatgtc
     5341 tacccagaac ggcttcatct tttacatccc tgcctggctg acatccaaga tcgatccatc
     5401 taccggcttt gtgaacctgc tgaaaaccaa gtataccagc atcgccgatt ccaagaagtt
     5461 catcagctcc tttgacagga tcatgtacgt gcccgaggag gatctgttcg agtttgccct
     5521 ggactataag aacttctctc gcacagacgc cgattacatc aagaagtgga agctgtactc
     5581 ctacggcaac cggatcagaa tcttccggaa tcctaagaag aacaacgtgt tcgactggga
     5641 ggaggtgtgc ctgaccagcg cctataagga gctgttcaac aagtacggca tcaattatca
     5701 gcagggcgat atcagagccc tgctgtgcga gcagtccgac aaggccttct actctagctt
     5761 tatggccctg atgagcctga tgctgcagat gcggaacagc atcacaggcc gcaccgacgt
     5821 ggattttctg atcagccctg tgaagaactc cgacggcatc ttctacgata gccggaacta
     5881 tgaggcccag gagaatgcca tcctgccaaa gaacgccgac gccaatggcg cctataacat
     5941 cgccagaaag gtgctgtggg ccatcggcca gttcaagaag gccgaggacg agaagctgga
     6001 taaggtgaag atcgccatct ctaacaagga gtggctggag tacgcccaga ccagcgtgaa
     6061 gcacggatcc tatccctatg acgtgcccga ttatgccagc ctgggcagcg gctcccctgc
     6121 tgctaaacgt gttaagcttg atgggggtag cccggcagcc aagagagtca aactcgacga
     6181 cggcattggt agtgggagca acggcagcag cctcgatgct ttagacgatt ttgacttaga
     6241 tatgcttggt tcagacgcgt tagacgactt cgacctagac atgttaggct cagatgcatt
     6301 ggacgacttc gatttagata tgttgggctc cgatgcccta gatgactttg atctagatat
     6361 gctaggtagt cccaaaaaga agaggaaagt gggatccgtc cttccgcaag cgcctgcccc
     6421 agcgcctgca ccggcaatgg tgtccgccct cgcacaggcc cctgcgcccg tccccgtgct
     6481 cgcgcctgga ccgccccagg cggtcgctcc accggctccg aagccgacgc aggccggaga
     6541 gggaacactc tccgaagcac ttcttcaact ccagtttgat gacgaggatc ttggagcact
     6601 ccttggaaac tcgacagacc ctgcggtgtt taccgacctc gcgtcagtag ataactccga
     6661 atttcagcag cttttgaacc agggtatccc ggtcgcgcca catacaacgg agcccatgtt
     6721 gatggaatac cccgaagcaa tcacgagact tgtgacggga gcgcagcggc ctcccgatcc
     6781 cgcacccgca cctttggggg cacctggcct ccctaacgga cttttgagcg gcgacgagga
     6841 tttctcctcc atcgccgata tggatttctc agccttgctg tcaggctctg gcagcggcag
     6901 cgacctttcc catccgcccc caaggggcca tctggatgag ctgacaacca cacttgagtc
     6961 catgaccgag gatctgaacc tggactcacc cctgaccccg gaattgaacg agattctgga
     7021 taccttcctg aacgacgagt gcctcttgca tgccatgcat atcagcacag gactgtccat
     7081 cttcgacaca tctctgtttt agtgtacagg ccgcactcct caggtgcagg ctgcctatca
     7141 gaaggtggtg gctggtgtgg ccaatgccct ggctcacaaa taccactgag atctttttcc
     7201 ctctgccaaa aattatgggg acatcatgaa gccccttgag catctgactt ctggctaata
     7261 aaggaaattt attttcattg caatagtgtg ttggaatttt ttgtgtctct cactcggaag
     7321 gacatatggg agggcaaatc atttaaaaca tcagaatgag tatttggttt agagtttggc
     7381 aacatatgcc catatgctgg ctgccatgaa caaaggttgg ctataaagag gtcatcagta
     7441 tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga cttgaggtta
     7501 gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa aattttcctt
     7561 acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca tagctgtccc
     7621 tcttctctta tggagatccc tcgacctgca gcccaagctt ggcgtaatca tggtcatagc
     7681 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7741 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7801 cactgcccgc tttccagtcg ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc
     7861 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     7921 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     7981 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     8041 aaagctaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     8101 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     8161 gtatcttatc atgtctggat ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     8221 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8281 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8341 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8401 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8461 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8521 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8581 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8641 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8701 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8761 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8821 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8881 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8941 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9001 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9061 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9121 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9181 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9241 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9301 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9361 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9421 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9481 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9541 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9601 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9661 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9721 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9781 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9841 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9901 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9961 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10021 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10081 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10141 cgcg
//
